FastQCFastQC Report
Fri 27 May 2016
SRR2139530_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139530_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9790736
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA119300.052.591881
GTATCAA221300.038.5423361
TCAACGC262450.031.5966154
ATCAACG266550.031.2421343
CAACGCA269650.030.7717325
TATCAAC279250.030.1040862
AACGCAG278450.029.8307536
CTTATAC66550.026.2396561
ACGCAGA320000.025.9574177
CGCAGAG330850.025.1064198
GTACATG309300.022.7818371
TACATGG312150.022.0256522
GCAGAGT386100.021.4466539
TATACAC98300.021.3798733
ACATGGG314750.021.2303563
GAGTACT212350.020.02263612-13
GTGGTAT60900.019.452241
CAGAGTA377500.019.16858510-11
TTATACA100150.018.9696832
AGAGTAC346200.018.82286510-11
Produced by FastQC (version 0.11.5)