##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139530_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9790736
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.633076308052836	33.0	33.0	33.0	33.0	33.0
2	32.63647063918382	33.0	33.0	33.0	33.0	33.0
3	32.63651874588386	33.0	33.0	33.0	33.0	33.0
4	36.54640335517166	37.0	37.0	37.0	37.0	37.0
5	36.567859760491956	37.0	37.0	37.0	37.0	37.0
6	36.550803535096854	37.0	37.0	37.0	37.0	37.0
7	36.51354300636847	37.0	37.0	37.0	37.0	37.0
8	36.51514789082251	37.0	37.0	37.0	37.0	37.0
9	36.55486410827542	37.0	37.0	37.0	37.0	37.0
10-11	36.47942126107782	37.0	37.0	37.0	37.0	37.0
12-13	36.47758263525847	37.0	37.0	37.0	37.0	37.0
14-15	38.5685518432935	40.0	37.0	40.0	37.0	40.0
16-17	38.50823293570575	40.0	37.0	40.0	37.0	40.0
18-19	38.52260836161858	40.0	37.0	40.0	37.0	40.0
20-21	38.50279048704867	40.0	37.0	40.0	37.0	40.0
22-23	38.51279113595685	40.0	37.0	40.0	37.0	40.0
24-25	38.50060588251767	40.0	37.0	40.0	37.0	40.0
26-27	38.40023826933616	40.0	37.0	40.0	37.0	40.0
28-29	38.338901222542866	40.0	37.0	40.0	37.0	40.0
30-31	38.28544264098457	40.0	37.0	40.0	37.0	40.0
32-33	38.22465742717567	40.0	37.0	40.0	37.0	40.0
34-35	38.18832658633228	40.0	37.0	40.0	37.0	40.0
36-37	38.12976960625675	40.0	37.0	40.0	37.0	40.0
38-39	38.078865768772275	40.0	37.0	40.0	33.0	40.0
40-41	38.05092395786818	40.0	37.0	40.0	33.0	40.0
42-43	37.99445349427222	40.0	37.0	40.0	33.0	40.0
44-45	37.927716267719276	40.0	37.0	40.0	33.0	40.0
46-47	37.88530408096135	40.0	37.0	40.0	33.0	40.0
48-49	37.861953081763794	40.0	37.0	40.0	33.0	40.0
50-51	37.77787074272885	40.0	37.0	40.0	33.0	40.0
52-53	37.67310008250352	40.0	37.0	40.0	33.0	40.0
54-55	37.4764334597952	40.0	37.0	40.0	33.0	40.0
56-57	37.39120867643342	40.0	37.0	40.0	33.0	40.0
58-59	37.273540202000206	38.5	37.0	40.0	33.0	40.0
60-61	37.12968584447262	37.0	37.0	40.0	33.0	40.0
62-63	36.964999237061875	37.0	37.0	40.0	33.0	40.0
64-65	36.77684430674198	37.0	37.0	40.0	33.0	40.0
66-67	36.56644469896307	37.0	37.0	40.0	33.0	40.0
68-69	36.360612161976576	37.0	37.0	40.0	33.0	40.0
70-71	36.09020102095266	37.0	37.0	38.5	33.0	40.0
72-73	35.87008369239716	37.0	37.0	37.0	33.0	40.0
74-75	35.67635266349379	37.0	33.0	37.0	33.0	40.0
76-77	34.05002503070569	35.0	33.0	37.0	30.0	37.0
78-79	35.144497863479444	37.0	33.0	37.0	33.0	37.0
80-81	35.2029022991498	37.0	33.0	37.0	33.0	37.0
82-83	35.08364425066703	37.0	33.0	37.0	33.0	37.0
84-85	34.91791173403045	37.0	33.0	37.0	33.0	37.0
86-87	34.83874495436867	37.0	33.0	37.0	33.0	37.0
88-89	34.7384381422558	37.0	33.0	37.0	33.0	37.0
90-91	34.62760669972579	37.0	33.0	37.0	33.0	37.0
92-93	34.60060722040316	37.0	33.0	37.0	33.0	37.0
94-95	34.63062758029696	37.0	33.0	37.0	33.0	37.0
96-97	34.62368257821339	37.0	33.0	37.0	33.0	37.0
98-99	34.61812216733834	37.0	33.0	37.0	33.0	37.0
100	34.6291316090711	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	14.0
20	70.0
21	378.0
22	1420.0
23	4049.0
24	9426.0
25	18246.0
26	32068.0
27	50523.0
28	73715.0
29	103576.0
30	140032.0
31	186063.0
32	245901.0
33	328366.0
34	451341.0
35	658827.0
36	1162239.0
37	2844892.0
38	3408817.0
39	70773.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.56534299362173	20.229204423446816	14.327697121033598	23.87775546189786
2	14.534269946610756	21.173740156000527	41.62082401159627	22.671165885792448
3	17.781604978420418	26.70037267882619	30.623029770182754	24.894992572570644
4	12.33736666988059	18.463443402007776	39.030395671990334	30.168794256121295
5	13.297703053171897	37.91559694797204	35.587539077756766	13.199160921099292
6	29.507736097963743	38.110470686335354	19.114459768245943	13.267333447454957
7	26.31308820909889	32.71104439952216	23.369233937060503	17.606633454318448
8	24.50339790593884	36.50314950786131	21.276888683343113	17.71656390285674
9	25.71718816644632	16.806274829594017	21.087066386020417	36.389470617939246
10-11	24.12253813139758	26.683209355943365	29.060667240980955	20.1335852716781
12-13	25.126996371313382	24.275936453171404	28.94800261369617	21.64906456181905
14-15	22.271233218117487	25.61510757564615	26.488122962272936	25.625536243963428
16-17	21.850732161504506	28.749774276418034	27.110857651559595	22.28863591051786
18-19	21.926109538649598	27.87502900701234	28.767847483580393	21.43101397075766
20-21	22.703042882919984	27.076887045200266	28.610718043723534	21.609352028156223
22-23	22.457079508707288	27.056666672795465	28.469778789165833	22.01647502933141
24-25	22.266771248186913	27.24252398355472	28.522757080132312	21.967947688126056
26-27	22.179126345077034	27.42616137240382	28.485397160536426	21.909315121982726
28-29	22.07727024907508	27.254546233367744	28.633349789284424	22.03483372827275
30-31	22.305861324913778	27.322180013034487	28.424494606647997	21.94746405540374
32-33	22.30654880218882	27.312083561130706	28.391561347095983	21.98980628958449
34-35	22.32340174803155	27.313190611811045	28.403305749493292	21.96010189066411
36-37	22.257871656068755	27.351328258177745	28.299423201674806	22.0913768840787
38-39	22.133687666655373	27.36058789942547	28.447158213429603	22.058566220489553
40-41	22.341601397502266	27.235493620424144	28.350727352387732	22.072177629685857
42-43	22.29558300567913	27.29538973163665	28.162648389923035	22.246378872761184
44-45	22.31612991823642	27.450757971298263	27.9951446831299	22.237967427335423
46-47	22.520485023412924	27.371721271507383	27.635009538852863	22.472784166226834
48-49	22.403656467874026	27.445936619893217	27.67237754050813	22.478029371724624
50-51	22.37480991827613	27.610668662403597	27.59104887227293	22.423472547047343
52-53	22.53408189950462	27.555757086277545	27.56020198611422	22.349959028103616
54-55	22.559018185678084	27.479259511905475	27.48825240003427	22.473469902382174
56-57	22.526102289777963	27.654055402452414	27.37805796829283	22.44178433947679
58-59	22.42463858427103	27.678974932705845	27.519141948093246	22.377244534929883
60-61	22.551105050432078	27.775904675200085	27.336266701498886	22.336723572868955
62-63	22.48255505844312	27.858277671908287	27.346309077985687	22.312858191662905
64-65	22.49442892123271	27.679263606469323	27.337985286483818	22.488322185814148
66-67	22.552829700553445	27.674898049807233	27.235314432449254	22.53695781719007
68-69	22.465608768345582	27.66819712680273	27.353951538507843	22.512242566343843
70-71	22.528469795279506	27.451315149570192	27.424467338812303	22.595747716338003
72-73	22.552522307865768	27.520180108202236	27.282975702118723	22.644321881813273
74-75	22.460823319306016	27.669515442307308	27.254213178984738	22.61544805940193
76-77	22.5749781925974	27.57845503592123	27.240089958491254	22.60647681299012
78-79	22.537024275291433	27.54830180945641	27.18627699713954	22.72839691811262
80-81	22.49223745910401	27.6440079476536	27.196076721745516	22.66767787149687
82-83	22.628501226301097	27.54013873886102	27.257776077985586	22.5735839568523
84-85	22.59982799394661	27.574822069405762	27.21934961865424	22.606000317993388
86-87	22.515315802865313	27.595420445449925	27.28475006229437	22.604513689390384
88-89	22.55685127256427	27.57027366874969	27.259816672770498	22.613058385915544
90-91	22.568358973147774	27.613016102668663	27.25601229522866	22.562612628954902
92-93	22.5947598483599	27.585815162669963	27.27635811471193	22.54306687425821
94-95	22.44741330460315	27.573237446720594	27.326099696792895	22.65324955188336
96-97	22.575337949600296	27.6964261538234	27.34964360391423	22.378592292662074
98-99	22.74828431076346	28.430767650410814	26.492686416512228	22.328261622313498
100	20.849888431125343	28.210308647510935	27.59791949105603	23.341883430307696
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2176.0
1	1881.0
2	2357.0
3	3258.5
4	4231.5
5	6842.5
6	9038.0
7	10509.0
8	11426.5
9	11163.5
10	10321.0
11	9477.5
12	10335.0
13	13779.0
14	21911.5
15	33159.5
16	42577.5
17	48551.5
18	50095.0
19	46895.0
20	41161.5
21	35512.5
22	31689.5
23	31731.5
24	36623.5
25	46218.0
26	59443.0
27	75403.5
28	93953.0
29	115168.5
30	137255.5
31	160371.5
32	186310.5
33	213164.0
34	238709.5
35	262871.0
36	288088.0
37	308901.5
38	323987.0
39	339845.5
40	355080.0
41	367070.0
42	372859.5
43	377888.0
44	384024.5
45	389899.0
46	397098.0
47	400483.5
48	399003.5
49	395654.0
50	391037.5
51	380395.5
52	364193.0
53	344095.5
54	321041.5
55	297043.0
56	272170.0
57	245551.0
58	218794.5
59	191532.0
60	160781.0
61	130446.0
62	103684.0
63	79774.0
64	59097.5
65	43414.5
66	32647.5
67	25290.0
68	19708.0
69	14699.5
70	10714.0
71	7877.0
72	5546.5
73	3852.5
74	2581.5
75	1661.0
76	1067.5
77	653.5
78	422.5
79	300.5
80	216.0
81	143.0
82	96.5
83	63.0
84	42.0
85	30.5
86	23.5
87	18.5
88	10.5
89	5.0
90	3.5
91	3.0
92	4.0
93	3.5
94	2.0
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	1.2256484088632357E-4
7	0.0
8	0.0
9	0.0
10-11	0.005209005737668751
12-13	3.5748078591844374E-5
14-15	0.004248914484059217
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	131.0
20-21	508.0
22-23	1179.0
24-25	2567.0
26-27	3449.0
28-29	5891.0
30-31	12993.0
32-33	17049.0
34-35	23233.0
36-37	32487.0
38-39	39036.0
40-41	42283.0
42-43	51904.0
44-45	62644.0
46-47	60263.0
48-49	52638.0
50-51	46168.0
52-53	44710.0
54-55	49921.0
56-57	53400.0
58-59	53127.0
60-61	53276.0
62-63	53706.0
64-65	55961.0
66-67	62222.0
68-69	56881.0
70-71	56769.0
72-73	55025.0
74-75	57504.0
76-77	56327.0
78-79	61467.0
80-81	63102.0
82-83	62375.0
84-85	64193.0
86-87	68224.0
88-89	74412.0
90-91	79417.0
92-93	85218.0
94-95	109083.0
96-97	334130.0
98-99	43103.0
100-101	7582760.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.59708654470055
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.45170275786526	75.67353439777955
2	7.375521607799089	12.478952795345768
3	1.7521345113750846	4.446764246902638
4	0.6480296971687368	2.1928569749967886
5	0.30967732229729217	1.3098899617657573
6	0.14617672218680788	0.741967489059482
7	0.08931101452322568	0.5288816137511431
8	0.05716807887464175	0.3869002332921868
9	0.03868375589453146	0.29452797407553727
>10	0.12828223925922908	1.6663421526063174
>50	0.00264531355388984	0.15153666565725807
>100	6.058026827656291E-4	0.0844588039738322
>500	4.0784429939286905E-5	0.0248589947511751
>1k	2.0392089536813474E-5	0.018527696042627002
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	2.0427473481053928E-5	0.0	0.0	0.0
3	0.0	2.0427473481053928E-5	0.0	1.0213736740526964E-5	0.0
4	0.0	8.170989392421571E-5	0.0	2.0427473481053928E-5	0.0
5	0.0	1.0213736740526963E-4	0.0	3.064121022158089E-5	0.0
6	0.0	1.0213736740526963E-4	0.0	3.064121022158089E-5	0.0
7	0.0	1.0213736740526963E-4	0.0	3.064121022158089E-5	0.0
8	0.0	1.3277857762685052E-4	0.0	4.0854946962107855E-5	0.0
9	0.0	1.8384726132948534E-4	0.0	4.0854946962107855E-5	0.0
10-11	0.0	1.8895412969974882E-4	0.0	5.106868370263482E-5	0.0
12-13	0.0	1.991678664402758E-4	0.0	5.106868370263482E-5	0.0
14-15	0.0	2.75770891994228E-4	0.0	7.660302555395222E-5	5.106868370263482E-6
16-17	0.0	2.75770891994228E-4	0.0	9.703049903500615E-5	1.0213736740526964E-5
18-19	1.0213736740526964E-5	3.064121022158089E-4	0.0	1.123511041457966E-4	1.5320605110790447E-5
20-21	1.0213736740526964E-5	3.370533124373898E-4	0.0	1.123511041457966E-4	2.0427473481053928E-5
22-23	1.0213736740526964E-5	3.7280139102923414E-4	0.0	1.1745797251606008E-4	4.0854946962107855E-5
24-25	1.0213736740526964E-5	4.0344260125081504E-4	0.0	1.2256484088632357E-4	5.61755520728983E-5
26-27	1.0213736740526964E-5	4.0854946962107854E-4	0.0	1.429923143673775E-4	6.128242044316179E-5
28-29	1.0213736740526964E-5	4.0854946962107854E-4	0.0	3.472670491779168E-4	6.128242044316179E-5
30-31	1.0213736740526964E-5	4.289769431021325E-4	0.0	0.0010162668056824327	6.128242044316179E-5
32-33	1.5320605110790447E-5	4.289769431021325E-4	0.0	0.003064121022158089	6.128242044316179E-5
34-35	2.0427473481053928E-5	4.953662319155577E-4	0.0	0.005826936810470633	6.128242044316179E-5
36-37	2.0427473481053928E-5	5.770761258397734E-4	0.0	0.00890637843773951	7.149615718368875E-5
38-39	2.0427473481053928E-5	6.128242044316178E-4	0.0	0.013579162996530596	8.68167622944792E-5
40-41	2.0427473481053928E-5	6.843203616153066E-4	0.0	0.017884253032662712	9.192363066474267E-5
42-43	2.0427473481053928E-5	7.404959136882048E-4	0.0	0.022271052962719043	9.192363066474267E-5
44-45	2.0427473481053928E-5	8.681676229447919E-4	0.0	0.026908089442918285	9.703049903500615E-5
46-47	2.0427473481053928E-5	9.498775168690076E-4	0.0	0.03169322510585516	1.3277857762685055E-4
48-49	2.0427473481053928E-5	9.549843852392711E-4	0.0	0.03622301735027888	1.6341978784843142E-4
50-51	2.0427473481053928E-5	0.001092869831236385	0.0	0.040446397492486776	1.7363352458895837E-4
52-53	2.0427473481053928E-5	0.001103083567976912	0.0	0.044920014184837584	1.7874039295922184E-4
54-55	2.0427473481053928E-5	0.001103083567976912	0.0	0.04904125695964022	1.8895412969974882E-4
56-57	2.0427473481053928E-5	0.001113297304717439	0.0	0.05255988926675176	1.940609980700123E-4
58-59	2.0427473481053928E-5	0.0011184041730877025	0.0	0.05613469712593619	1.940609980700123E-4
60-61	2.0427473481053928E-5	0.0011541522516795469	0.0	0.05988824537807985	1.940609980700123E-4
62-63	2.0427473481053928E-5	0.0011899003302713912	0.0	0.06358051120978034	1.940609980700123E-4
64-65	2.0427473481053928E-5	0.0013073583027874514	0.0	0.06694593746578398	1.940609980700123E-4
66-67	2.0427473481053928E-5	0.0013328926446387686	0.0	0.07067905824444659	1.940609980700123E-4
68-69	2.0427473481053928E-5	0.001389068196711667	0.0	0.07455517133747658	1.940609980700123E-4
70-71	2.0427473481053928E-5	0.0014554574855250922	0.0	0.07792570446185046	2.0938160318080274E-4
72-73	2.0427473481053928E-5	0.0014707780906358828	0.0	0.08051999359394432	2.3491594503212017E-4
74-75	2.0427473481053928E-5	0.001526953642708781	0.0	0.08293554233307895	2.3491594503212017E-4
76-77	2.0427473481053928E-5	0.0016086635366329967	0.0	0.08497828968118434	2.4512968177264714E-4
78-79	2.0427473481053928E-5	0.0016546253519653682	0.0	0.08649502958715258	2.553434185131741E-4
80-81	2.0427473481053928E-5	0.0016546253519653682	0.0	0.08714360187017606	2.6045028688343754E-4
82-83	2.0427473481053928E-5	0.0016699459570761585	0.0	0.08753172386631608	2.6555715525370104E-4
84-85	3.064121022158089E-5	0.0017005871672977394	0.0	0.08765939557557266	2.6555715525370104E-4
86-87	4.0854946962107855E-5	0.0017669764561111646	0.0	0.08777174667971846	2.6555715525370104E-4
88	4.0854946962107855E-5	0.0018486863500353804	0.0	0.08777685354808873	2.85984628734755E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11930	0.0	52.59188	1
GTATCAA	22130	0.0	38.542336	1
TCAACGC	26245	0.0	31.596615	4
ATCAACG	26655	0.0	31.242134	3
CAACGCA	26965	0.0	30.771732	5
TATCAAC	27925	0.0	30.104086	2
AACGCAG	27845	0.0	29.830753	6
CTTATAC	6655	0.0	26.239656	1
ACGCAGA	32000	0.0	25.957417	7
CGCAGAG	33085	0.0	25.106419	8
GTACATG	30930	0.0	22.781837	1
TACATGG	31215	0.0	22.025652	2
GCAGAGT	38610	0.0	21.446653	9
TATACAC	9830	0.0	21.379873	3
ACATGGG	31475	0.0	21.230356	3
GAGTACT	21235	0.0	20.022636	12-13
GTGGTAT	6090	0.0	19.45224	1
CAGAGTA	37750	0.0	19.168585	10-11
TTATACA	10015	0.0	18.969683	2
AGAGTAC	34620	0.0	18.822865	10-11
>>END_MODULE