FastQCFastQC Report
Fri 27 May 2016
SRR2139529_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139529_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8564266
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA124200.049.778341
GTATCAA217750.036.4592441
TCAACGC262300.029.8052084
ATCAACG264500.029.5244143
CAACGCA268800.029.0226335
TATCAAC273500.028.805962
AACGCAG273900.028.6717996
ACGCAGA314300.024.9450367
CGCAGAG326550.024.0624058
GTACATG273800.021.142311
GCAGAGT373950.020.9911989
CTTATAC86900.020.960041
TACATGG277650.020.6309912
GAGTACT212550.020.44851112-13
GTGGTAT61350.019.8162521
ACATGGG280650.019.8092423
TGGTATC57650.019.6621842
CAGAGTA362350.019.19109710-11
GTACTTT236450.018.4641714-15
AGAGTAC339650.018.33969910-11
Produced by FastQC (version 0.11.5)