##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139529_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8564266
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.637188522635796	33.0	33.0	33.0	33.0	33.0
2	32.637771292951435	33.0	33.0	33.0	33.0	33.0
3	32.63565657582331	33.0	33.0	33.0	33.0	33.0
4	36.54447958529079	37.0	37.0	37.0	37.0	37.0
5	36.56698694318929	37.0	37.0	37.0	37.0	37.0
6	36.55193147900824	37.0	37.0	37.0	37.0	37.0
7	36.51497828301923	37.0	37.0	37.0	37.0	37.0
8	36.514890593075926	37.0	37.0	37.0	37.0	37.0
9	36.5551643304867	37.0	37.0	37.0	37.0	37.0
10-11	36.479546758589706	37.0	37.0	37.0	37.0	37.0
12-13	36.47941977748006	37.0	37.0	37.0	37.0	37.0
14-15	38.55323246615647	40.0	37.0	40.0	37.0	40.0
16-17	38.4918502064275	40.0	37.0	40.0	37.0	40.0
18-19	38.50871913600068	40.0	37.0	40.0	37.0	40.0
20-21	38.491334248679365	40.0	37.0	40.0	37.0	40.0
22-23	38.501529962466186	40.0	37.0	40.0	37.0	40.0
24-25	38.48889980263074	40.0	37.0	40.0	37.0	40.0
26-27	38.38424141619355	40.0	37.0	40.0	37.0	40.0
28-29	38.32081957685681	40.0	37.0	40.0	37.0	40.0
30-31	38.26027721691422	40.0	37.0	40.0	37.0	40.0
32-33	38.197285468377146	40.0	37.0	40.0	37.0	40.0
34-35	38.16182777061702	40.0	37.0	40.0	37.0	40.0
36-37	38.09898230036464	40.0	37.0	40.0	33.0	40.0
38-39	38.04739731231283	40.0	37.0	40.0	33.0	40.0
40-41	38.01741975502337	40.0	37.0	40.0	33.0	40.0
42-43	37.96436575086348	40.0	37.0	40.0	33.0	40.0
44-45	37.899850565579456	40.0	37.0	40.0	33.0	40.0
46-47	37.85574740461094	40.0	37.0	40.0	33.0	40.0
48-49	37.83373315156733	40.0	37.0	40.0	33.0	40.0
50-51	37.74588107118791	40.0	37.0	40.0	33.0	40.0
52-53	37.636108692710465	40.0	37.0	40.0	33.0	40.0
54-55	37.44018681556129	40.0	37.0	40.0	33.0	40.0
56-57	37.35590955593868	40.0	37.0	40.0	33.0	40.0
58-59	37.236760388294684	37.0	37.0	40.0	33.0	40.0
60-61	37.095747011640306	37.0	37.0	40.0	33.0	40.0
62-63	36.92981520957002	37.0	37.0	40.0	33.0	40.0
64-65	36.745184174773925	37.0	37.0	40.0	33.0	40.0
66-67	36.53670659358178	37.0	37.0	40.0	33.0	40.0
68-69	36.33318545637178	37.0	37.0	40.0	33.0	40.0
70-71	36.0638197439001	37.0	37.0	38.5	33.0	40.0
72-73	35.84954601033591	37.0	37.0	37.0	33.0	40.0
74-75	35.65925345181748	37.0	33.0	37.0	33.0	40.0
76-77	34.02518855569423	35.0	33.0	37.0	30.0	37.0
78-79	35.12788664785397	37.0	33.0	37.0	33.0	37.0
80-81	35.190653656600006	37.0	33.0	37.0	33.0	37.0
82-83	35.070570943589786	37.0	33.0	37.0	33.0	37.0
84-85	34.90870716561413	37.0	33.0	37.0	33.0	37.0
86-87	34.83167837722105	37.0	33.0	37.0	33.0	37.0
88-89	34.73266511122893	37.0	33.0	37.0	33.0	37.0
90-91	34.62205712944409	37.0	33.0	37.0	33.0	37.0
92-93	34.59807862551325	37.0	33.0	37.0	33.0	37.0
94-95	34.63151980444485	37.0	33.0	37.0	33.0	37.0
96-97	34.62536971185688	37.0	33.0	37.0	33.0	37.0
98-99	34.60350603755231	37.0	33.0	37.0	33.0	37.0
100	34.587637430367984	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	0.0
19	10.0
20	64.0
21	317.0
22	1165.0
23	3563.0
24	7959.0
25	15659.0
26	27552.0
27	43565.0
28	64347.0
29	89849.0
30	121640.0
31	162705.0
32	215665.0
33	288691.0
34	399190.0
35	580064.0
36	1022050.0
37	2477125.0
38	2969810.0
39	73275.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.58711324473107	20.151207353905168	14.50339118378621	23.758288217577547
2	14.577116124137198	20.9422850714819	41.703293662294	22.7773051420869
3	17.969549287703117	26.4221475605732	30.58020383766688	25.0280993140568
4	12.36620861612659	18.334834532229614	38.7425145365639	30.55644231507989
5	13.324819663471452	37.81980849263673	35.49149454255625	13.363877301335572
6	29.77201516909704	37.83584653140834	18.88694566975711	13.505192629737511
7	26.386347644970392	32.78111632683992	23.0099812406574	17.822554787532287
8	24.580553663326196	36.47301473354517	21.073446340877318	17.872985262251312
9	25.657587001618122	16.858362409574852	20.924361760832745	36.559688827974284
10-11	24.25982797396489	26.613468055143613	28.769205798347	20.357498172544496
12-13	25.31066884439807	24.182200595404346	28.752400673309463	21.754729886888118
14-15	22.35192837867661	25.57275690016149	26.389642372135224	25.68567234902667
16-17	21.878635016707797	28.742755070895743	27.01480780723065	22.363802105165814
18-19	21.95561184110816	27.689827709695148	28.85473781407537	21.49982263512133
20-21	22.716758635446617	26.868224399736224	28.788210247549422	21.62680671726774
22-23	22.468854557364736	26.966087906324777	28.600615309590665	21.96444222671982
24-25	22.345507173114846	27.109653091935566	28.67318165614928	21.871658078800312
26-27	22.245794866084964	27.29711380409273	28.61310758429516	21.84398374552714
28-29	22.172373866679635	27.173372067580303	28.704277957105884	21.949976108634182
30-31	22.277864959002596	27.204420114419435	28.657816821864458	21.859898104713512
32-33	22.192777769706524	27.33063099964242	28.568127912388057	21.908463318263003
34-35	22.40134556957634	27.20213368621624	28.585699143880532	21.810821600326882
36-37	22.203696208567774	27.304634757665358	28.42316392745341	22.06850510631346
38-39	22.107546070637547	27.222938997425754	28.560526641813205	22.10898829012349
40-41	22.31618930981053	27.09718262830108	28.51708281034916	22.069545251539232
42-43	22.271279527107822	27.157059571883202	28.34562356017742	22.226037340831553
44-45	22.26189142034244	27.370380641425978	28.082966003996628	22.284761934234954
46-47	22.526822923558324	27.28220959689932	27.662624543383068	22.52834293615929
48-49	22.38010678865879	27.420859459519146	27.702921298987686	22.496112452834378
50-51	22.375604802507127	27.56253627832473	27.5999599111462	22.461899008021945
52-53	22.50042255686022	27.540215154775616	27.595812954009325	22.36354933435484
54-55	22.496809633308267	27.488779454417333	27.492660867979712	22.52175004429469
56-57	22.57404469921563	27.62155226707633	27.336434971096008	22.467968062612034
58-59	22.35441608410734	27.7197423266675	27.5422128255434	22.38362876368176
60-61	22.54519385969884	27.82705165851254	27.307872125371517	22.319882356417097
62-63	22.422567051408965	27.926603479351957	27.31708005672576	22.333749412513313
64-65	22.44786715809106	27.76071699365889	27.311891095815593	22.479524752434457
66-67	22.48158634121567	27.750546366432783	27.165576616840887	22.60229067551066
68-69	22.465959091079878	27.680217134961683	27.273743670617197	22.580080103341242
70-71	22.47227456500895	27.512484010362982	27.312844115830075	22.702397308797995
72-73	22.53794877911882	27.51258129267706	27.21121703063331	22.73825289757081
74-75	22.47931038366908	27.657735360644857	27.179375983256282	22.683578272429774
76-77	22.581041094705313	27.556533775627585	27.14068661046069	22.72173851920641
78-79	22.57608846178171	27.53884228535538	27.066633935756546	22.81843531710636
80-81	22.524934539870095	27.647005750918947	27.07262216542302	22.755437543787938
82-83	22.622188362703735	27.558152439040896	27.16133951827085	22.65831967998452
84-85	22.673318613381056	27.565123042057483	27.123580503491844	22.63797784106962
86-87	22.581874274576595	27.588508989329814	27.171968653579526	22.657648082514065
88-89	22.622045304439407	27.555173952624905	27.162421234894058	22.660359508041626
90-91	22.662549226987398	27.655448989831537	27.104677060133632	22.577324723047436
92-93	22.697210968840842	27.545984051806027	27.200473205671134	22.556331773681997
94-95	22.51314954132879	27.585985224695854	27.27488803415781	22.625977199817545
96-97	22.640904561798727	27.720577349413926	27.299427962230816	22.33909012655653
98-99	23.74435279496455	29.60695061211312	27.500921409709523	19.14777518321281
100	22.674450611920545	30.57716907717985	21.32451877786178	25.42386153303783
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2057.0
1	1814.0
2	2335.0
3	3307.5
4	4474.0
5	7110.0
6	9253.5
7	10636.5
8	11496.5
9	11298.5
10	10466.5
11	9668.0
12	10633.0
13	14134.0
14	22514.5
15	34393.0
16	44510.5
17	50729.5
18	52092.5
19	48984.0
20	43527.5
21	37722.0
22	33879.5
23	33909.5
24	37935.0
25	46206.5
26	57788.0
27	71109.5
28	86404.5
29	104754.0
30	124215.0
31	142951.0
32	163302.0
33	184167.5
34	205014.0
35	224470.5
36	244704.0
37	263618.5
38	278663.0
39	292545.5
40	305103.5
41	315668.0
42	321482.5
43	327642.5
44	335066.0
45	342285.5
46	350439.0
47	357075.5
48	357744.5
49	354261.0
50	350874.0
51	342021.5
52	326190.0
53	307853.0
54	289227.5
55	269438.5
56	247340.5
57	223221.0
58	198242.5
59	172042.0
60	143807.5
61	116993.5
62	92960.5
63	71527.5
64	53221.5
65	39442.5
66	29927.0
67	22793.0
68	17476.5
69	13085.5
70	9484.0
71	6915.0
72	4921.5
73	3257.0
74	2196.0
75	1499.0
76	988.0
77	635.5
78	395.0
79	245.0
80	176.5
81	117.0
82	81.0
83	55.0
84	38.0
85	29.5
86	22.5
87	17.0
88	11.5
89	6.0
90	4.0
91	3.5
92	2.5
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	1.4011708650805567E-4
7	0.0
8	0.0
9	0.0
10-11	0.005207685048549403
12-13	2.3352847751342615E-5
14-15	0.003923278422225559
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	131.0
20-21	530.0
22-23	1188.0
24-25	2521.0
26-27	3327.0
28-29	5545.0
30-31	12318.0
32-33	15721.0
34-35	22515.0
36-37	32237.0
38-39	38059.0
40-41	42093.0
42-43	52066.0
44-45	63381.0
46-47	62632.0
48-49	55675.0
50-51	46974.0
52-53	44183.0
54-55	50600.0
56-57	55354.0
58-59	56458.0
60-61	58433.0
62-63	60029.0
64-65	61770.0
66-67	66797.0
68-69	61734.0
70-71	61441.0
72-73	58545.0
74-75	59748.0
76-77	59567.0
78-79	64986.0
80-81	67015.0
82-83	65580.0
84-85	65123.0
86-87	69080.0
88-89	75188.0
90-91	79877.0
92-93	83671.0
94-95	101881.0
96-97	290024.0
98-99	541307.0
100-101	5848962.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.81112425032012
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.46820846530534	75.87899344604554
2	7.445835911162319	12.629794292181659
3	1.723880369769669	4.386126966996696
4	0.618755043816956	2.099092436066885
5	0.2826290623041725	1.198504425991532
6	0.15226571391243401	0.7748295829014683
7	0.08979994517745794	0.5331224015682123
8	0.057113755474898935	0.3875105449587244
9	0.035809081161934805	0.27333075885431857
>10	0.12282322248574502	1.611298815230475
>50	0.002318292543845241	0.13354335963056
>100	5.155130046451492E-4	0.06775390582921262
>500	4.5623880619618635E-5	0.026099063744635432
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	1.1676423875671308E-5	0.0	1.1676423875671308E-5	0.0
2	0.0	3.502927162701392E-5	0.0	1.1676423875671308E-5	0.0
3	0.0	3.502927162701392E-5	0.0	1.1676423875671308E-5	0.0
4	0.0	1.0508781488104176E-4	0.0	2.3352847751342615E-5	0.0
5	0.0	1.0508781488104176E-4	0.0	2.3352847751342615E-5	0.0
6	0.0	1.0508781488104176E-4	0.0	2.3352847751342615E-5	0.0
7	0.0	1.0508781488104176E-4	0.0	2.3352847751342615E-5	0.0
8	0.0	1.1676423875671307E-4	0.0	2.3352847751342615E-5	0.0
9	0.0	2.4520490138909743E-4	0.0	3.502927162701392E-5	0.0
10-11	0.0	2.4520490138909743E-4	0.0	3.502927162701392E-5	0.0
12-13	0.0	2.4520490138909743E-4	0.0	4.0867483564849574E-5	0.0
14-15	0.0	3.736455640214818E-4	0.0	5.8382119378356536E-5	0.0
16-17	0.0	3.853219878971531E-4	0.0	1.1676423875671307E-4	0.0
18-19	1.1676423875671308E-5	4.0867483564849575E-4	0.0	1.2844066263238438E-4	0.0
20-21	2.3352847751342615E-5	4.4370410727550966E-4	0.0	1.2844066263238438E-4	0.0
22-23	2.3352847751342615E-5	4.845715908403592E-4	0.0	1.3427887457022003E-4	0.0
24-25	2.3352847751342615E-5	5.487919221565514E-4	0.0	1.751463581350696E-4	0.0
26-27	2.3352847751342615E-5	5.954976176592366E-4	0.0	2.5104311332693307E-4	0.0
28-29	2.3352847751342615E-5	6.071740415349079E-4	0.0	5.954976176592366E-4	0.0
30-31	2.3352847751342615E-5	6.713943728511001E-4	0.0	0.0018507131842939021	0.0
32-33	2.3352847751342615E-5	6.772325847889358E-4	0.0	0.004979994782973812	0.0
34-35	2.3352847751342615E-5	7.648057638564706E-4	0.0	0.009393683007977566	0.0
36-37	3.5029271627013924E-5	8.582171548618411E-4	0.0	0.01598502428579402	0.0
38-39	4.670569550268523E-5	8.75731790675348E-4	0.0	0.03232034128785818	0.0
40-41	4.670569550268523E-5	9.691431816807184E-4	0.0	0.050547238957781085	0.0
42-43	4.670569550268523E-5	0.001010010665245568	0.0	0.06170406197098502	0.0
44-45	5.8382119378356536E-5	0.0011618041756292952	0.0	0.07555230068753119	0.0
46-47	5.8382119378356536E-5	0.0012844066263238438	0.0	0.08990262563073123	0.0
48-49	7.005854325402783E-5	0.001296083050199515	0.0	0.10389681964572328	0.0
50-51	7.005854325402783E-5	0.001465391196396749	0.0	0.11745898597731551	0.0
52-53	8.173496712969915E-5	0.0014829058322102559	0.0	0.1515833347539649	0.0
54-55	8.173496712969915E-5	0.0015062586799615986	0.0	0.1915984393758905	0.0
56-57	8.173496712969915E-5	0.0015296115277129412	0.0	0.2328512449286372	0.0
58-59	8.173496712969915E-5	0.0015471261635264483	0.0	0.2655860992640817	0.0
60-61	8.173496712969915E-5	0.0016113464948426404	0.0	0.29421085239528993	0.0
62-63	8.75731790675348E-5	0.0016463757664696543	0.0	0.31590564795628717	0.0
64-65	9.341139100537046E-5	0.0017631400052263674	0.0	0.3202843069096639	0.0
66-67	9.341139100537046E-5	0.0017631400052263674	0.0	0.32454620162428394	0.0
68-69	9.341139100537046E-5	0.0017981692768533812	0.0	0.32885480203440665	1.1676423875671308E-5
70-71	9.341139100537046E-5	0.0018390367604182308	0.0	0.3331458878087159	1.1676423875671308E-5
72-73	9.341139100537046E-5	0.0018507131842939021	0.0	0.3366780060311064	1.1676423875671308E-5
74-75	9.341139100537046E-5	0.0019090953036722588	0.0	0.3392643339195677	1.1676423875671308E-5
76-77	9.341139100537046E-5	0.0019733156349884505	0.0	0.34206667564972876	1.1676423875671308E-5
78-79	9.341139100537046E-5	0.0020316977543668076	0.0	0.34370721320426056	1.1676423875671308E-5
80-81	9.341139100537046E-5	0.0020433741782424786	0.0	0.34426184333835497	1.1676423875671308E-5
82-83	9.341139100537046E-5	0.002049212390180314	0.0	0.3446938710217548	1.1676423875671308E-5
84-85	9.341139100537046E-5	0.0020667270259938212	0.0	0.3448515027440764	1.1676423875671308E-5
86-87	9.341139100537046E-5	0.002090079873745164	0.0	0.3448982084395791	1.1676423875671308E-5
88	9.341139100537046E-5	0.0021951676886262056	0.0	0.3448982084395791	1.1676423875671308E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	12420	0.0	49.77834	1
GTATCAA	21775	0.0	36.459244	1
TCAACGC	26230	0.0	29.805208	4
ATCAACG	26450	0.0	29.524414	3
CAACGCA	26880	0.0	29.022633	5
TATCAAC	27350	0.0	28.80596	2
AACGCAG	27390	0.0	28.671799	6
ACGCAGA	31430	0.0	24.945036	7
CGCAGAG	32655	0.0	24.062405	8
GTACATG	27380	0.0	21.14231	1
GCAGAGT	37395	0.0	20.991198	9
CTTATAC	8690	0.0	20.96004	1
TACATGG	27765	0.0	20.630991	2
GAGTACT	21255	0.0	20.448511	12-13
GTGGTAT	6135	0.0	19.816252	1
ACATGGG	28065	0.0	19.809242	3
TGGTATC	5765	0.0	19.662184	2
CAGAGTA	36235	0.0	19.191097	10-11
GTACTTT	23645	0.0	18.46417	14-15
AGAGTAC	33965	0.0	18.339699	10-11
>>END_MODULE