FastQCFastQC Report
Fri 27 May 2016
SRR2139528_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139528_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8953939
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA125300.051.085951
GTATCAA225650.037.337431
TCAACGC269950.030.5372814
ATCAACG272550.030.357053
CAACGCA276050.029.8661545
TATCAAC284550.029.2896332
AACGCAG283000.029.2702546
ACGCAGA322400.025.6395157
CGCAGAG332050.024.894948
GTACATG290950.021.5843541
GCAGAGT385700.021.3323639
TACATGG293350.021.068662
GAGTACT218800.020.83617812-13
ACATGGG291200.020.644973
GTGGTAT60750.019.436011
TGGTATC57000.019.424772
CAGAGTA380050.019.08843810-11
AGAGTAC347700.018.88622710-11
GTACTTT244450.018.57027614-15
CATGGGG203000.018.4293864
Produced by FastQC (version 0.11.5)