##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139528_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8953939
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.64099666079923	33.0	33.0	33.0	33.0	33.0
2	32.64291235399303	33.0	33.0	33.0	33.0	33.0
3	32.642170892609386	33.0	33.0	33.0	33.0	33.0
4	36.55571129086316	37.0	37.0	37.0	37.0	37.0
5	36.577960828189696	37.0	37.0	37.0	37.0	37.0
6	36.56249969985277	37.0	37.0	37.0	37.0	37.0
7	36.52925801705819	37.0	37.0	37.0	37.0	37.0
8	36.52808869928643	37.0	37.0	37.0	37.0	37.0
9	36.566486995276605	37.0	37.0	37.0	37.0	37.0
10-11	36.49191903138943	37.0	37.0	37.0	37.0	37.0
12-13	36.49204165898382	37.0	37.0	37.0	37.0	37.0
14-15	38.58255355547988	40.0	37.0	40.0	37.0	40.0
16-17	38.52498743848936	40.0	37.0	40.0	37.0	40.0
18-19	38.54396042903576	40.0	37.0	40.0	37.0	40.0
20-21	38.52991557410021	40.0	37.0	40.0	37.0	40.0
22-23	38.53889812656461	40.0	37.0	40.0	37.0	40.0
24-25	38.52817335105999	40.0	37.0	40.0	37.0	40.0
26-27	38.42980890421248	40.0	37.0	40.0	37.0	40.0
28-29	38.36899801220066	40.0	37.0	40.0	37.0	40.0
30-31	38.31509395351232	40.0	37.0	40.0	37.0	40.0
32-33	38.25384500438993	40.0	37.0	40.0	37.0	40.0
34-35	38.224535494106135	40.0	37.0	40.0	37.0	40.0
36-37	38.169611260272816	40.0	37.0	40.0	37.0	40.0
38-39	38.12530671863119	40.0	37.0	40.0	37.0	40.0
40-41	38.094147963663985	40.0	37.0	40.0	35.0	40.0
42-43	38.03897491934648	40.0	37.0	40.0	33.0	40.0
44-45	37.97822898263711	40.0	37.0	40.0	33.0	40.0
46-47	37.93863587918749	40.0	37.0	40.0	33.0	40.0
48-49	37.91612533759869	40.0	37.0	40.0	33.0	40.0
50-51	37.84660333354216	40.0	37.0	40.0	33.0	40.0
52-53	37.74016993705957	40.0	37.0	40.0	33.0	40.0
54-55	37.54575996572824	40.0	37.0	40.0	33.0	40.0
56-57	37.47000099220135	40.0	37.0	40.0	33.0	40.0
58-59	37.350769104463126	40.0	37.0	40.0	33.0	40.0
60-61	37.213732877812994	37.0	37.0	40.0	33.0	40.0
62-63	37.05212148009285	37.0	37.0	40.0	33.0	40.0
64-65	36.862166985495875	37.0	37.0	40.0	33.0	40.0
66-67	36.65137463644828	37.0	37.0	40.0	33.0	40.0
68-69	36.44846277571293	37.0	37.0	40.0	33.0	40.0
70-71	36.18024967614488	37.0	37.0	38.5	33.0	40.0
72-73	35.962399596007515	37.0	37.0	37.0	33.0	40.0
74-75	35.76967179542693	37.0	35.0	37.0	33.0	40.0
76-77	34.13311045269557	35.0	33.0	37.0	30.0	37.0
78-79	35.21823107148241	37.0	33.0	37.0	33.0	37.0
80-81	35.26989955084637	37.0	33.0	37.0	33.0	37.0
82-83	35.144739165266294	37.0	33.0	37.0	33.0	37.0
84-85	34.97896106855006	37.0	33.0	37.0	33.0	37.0
86-87	34.898383485298204	37.0	33.0	37.0	33.0	37.0
88-89	34.79875183846582	37.0	33.0	37.0	33.0	37.0
90-91	34.68867709482129	37.0	33.0	37.0	33.0	37.0
92-93	34.66350166734895	37.0	33.0	37.0	33.0	37.0
94-95	34.691835886539906	37.0	33.0	37.0	33.0	37.0
96-97	34.685340897488985	37.0	33.0	37.0	33.0	37.0
98-99	34.66168661655668	37.0	33.0	37.0	33.0	37.0
100	34.643993534835666	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	2.0
17	1.0
18	2.0
19	11.0
20	46.0
21	259.0
22	1150.0
23	3172.0
24	7465.0
25	15017.0
26	26590.0
27	42319.0
28	62873.0
29	88444.0
30	120967.0
31	162012.0
32	216001.0
33	290115.0
34	402090.0
35	587963.0
36	1029879.0
37	2544358.0
38	3263832.0
39	89371.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.59685474739106	20.331778002954902	14.493140951708517	23.578226297945516
2	14.474869663507869	21.28748029219319	41.700373433412935	22.537276610886003
3	17.703247699141127	26.837897823516556	30.73994584953058	24.718908627811736
4	12.203031537293253	18.52411547588162	39.488408397689554	29.78444458913557
5	13.079640144968598	38.12163562874395	35.77240139786523	13.026322828422218
6	29.26464746234695	38.412559500455835	19.216403804231955	13.10638923296526
7	26.11450669923036	33.02315327365978	23.5808396729082	17.281500354201654
8	24.369810873181066	36.61781703002444	21.34859306055134	17.663779036243156
9	25.747048310246473	16.786634351652385	21.310453421672854	36.15586391642829
10-11	23.994345867308596	26.817625963441362	29.26727917537048	19.920748993879567
12-13	25.04865458051074	24.5361872142039	29.162968106986693	21.25219009829867
14-15	22.13750117690525	25.83405585729129	26.89453823263957	25.133904733163888
16-17	21.524543555635123	28.940938730987558	27.499349727533325	22.035167985843994
18-19	21.586421350424658	28.092049767147177	29.27607056514457	21.0454583172836
20-21	22.50961846293676	27.208530152779115	29.110634004648446	21.171217379635678
22-23	22.209213639554772	27.145870237600793	28.94009408064625	21.70482204219818
24-25	21.928325528957075	27.33875461548533	29.024029569492466	21.708890286065134
26-27	21.869111035581632	27.475817233978677	28.93013285260675	21.72493887783294
28-29	21.80073569391055	27.47219822807182	29.016527148437905	21.710538929579727
30-31	22.05554272846147	27.41322256393203	28.83019158419672	21.70104312340978
32-33	21.86887213218448	27.554508009368746	28.789492789477993	21.787127068968783
34-35	22.025560104350888	27.561117581848272	28.77573128782419	21.63759102597665
36-37	22.00628258026122	27.511388887527755	28.685538513598036	21.796790018612988
38-39	21.886423041475073	27.636481521113073	28.75188164173722	21.725213795674634
40-41	22.10754113475911	27.527352038929877	28.61775092967437	21.74735589663665
42-43	21.983926036017078	27.625482022062087	28.405820190506535	21.984771751414304
44-45	22.099788032708787	27.70252542571824	28.242391847199432	21.955294694373546
46-47	22.166500760858362	27.74072132400317	27.933973606215762	22.158804308922704
48-49	22.14764245546883	27.84818738202926	27.883267011092283	22.120903151409628
50-51	22.136708141554784	27.94282766167798	27.752240647791083	22.168223548976155
52-53	22.2940388223903	27.86947336700416	27.634071917193516	22.20241589341203
54-55	22.34303680212623	27.76561716237459	27.67681801960771	22.214528015891474
56-57	22.223722693436724	27.863914141054224	27.660835509482375	22.25152765602668
58-59	22.20347401302283	27.868748790936426	27.67823443230995	22.249542763730787
60-61	22.26442650007254	27.87013403243948	27.69308932602582	22.172350141462164
62-63	22.23579410228095	27.965105035961376	27.64157002279261	22.15753083896507
64-65	22.22956493542027	27.872636317118292	27.616165248471813	22.281633498989624
66-67	22.27882976768859	27.946766480652624	27.434885677845084	22.339518073813704
68-69	22.206972941650427	27.96552889912382	27.493750372876384	22.333747786349367
70-71	22.22851272720323	27.76627426908674	27.57144101487879	22.433771988831243
72-73	22.308212273200887	27.780987474634188	27.454211145951675	22.456589106213247
74-75	22.215622996647756	27.92088155676444	27.40574643601732	22.457749010570485
76-77	22.352731653143728	27.82296636634216	27.36739811478247	22.456903865731636
78-79	22.329394234886937	27.822395862360793	27.285952077427506	22.562257825324767
80-81	22.283310913675237	27.890961604707755	27.301721736193958	22.524005745423054
82-83	22.393172455167882	27.84005698710607	27.376431495086788	22.39033906263926
84-85	22.410138139931878	27.834045420680326	27.364874845769616	22.39094159361818
86-87	22.29928200535672	27.888668317718494	27.417070310100407	22.39497936682438
88-89	22.367778454401098	27.88641205775647	27.3519336840934	22.39387580374903
90-91	22.418639558126827	27.934500708054564	27.332160576450313	22.31469915736829
92-93	22.443112221943323	27.867557735700533	27.36472357762408	22.32460646473207
94-95	22.27340888614729	27.85811701972011	27.455491916267675	22.412982177864922
96-97	22.37569932748146	27.986188798076185	27.49917538795861	22.138936486483747
98-99	23.487376954201444	29.85201410691986	27.73109052588522	18.929518412993474
100	22.412788687637665	30.840085398507483	21.5810746290915	25.16605128476336
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2250.0
1	1970.5
2	2511.0
3	3479.0
4	4542.0
5	7254.5
6	9593.5
7	11072.0
8	11921.5
9	11744.0
10	10988.5
11	10191.0
12	11251.0
13	14987.0
14	23483.5
15	35353.0
16	45454.0
17	51658.0
18	52998.5
19	49899.5
20	44361.5
21	38978.0
22	35696.5
23	36445.0
24	41748.5
25	51552.5
26	65121.5
27	80856.0
28	99067.0
29	120424.5
30	142374.5
31	163574.5
32	187364.0
33	210640.0
34	232273.0
35	254350.5
36	276692.5
37	295523.0
38	310185.0
39	323778.5
40	336913.0
41	348000.5
42	352535.0
43	356942.5
44	361907.0
45	365162.5
46	366922.5
47	365006.0
48	361484.0
49	356138.5
50	348011.5
51	335484.5
52	320332.0
53	302391.5
54	282372.0
55	261954.5
56	239466.5
57	215913.0
58	191382.0
59	165444.0
60	138349.0
61	112461.5
62	89273.5
63	68589.0
64	50710.0
65	37272.5
66	28452.5
67	21964.5
68	16920.5
69	12361.5
70	8697.5
71	6349.0
72	4472.5
73	3011.0
74	2020.5
75	1361.0
76	938.0
77	589.0
78	357.0
79	238.5
80	166.0
81	120.0
82	88.0
83	68.0
84	51.0
85	33.0
86	21.5
87	15.0
88	10.0
89	5.5
90	3.0
91	2.5
92	3.0
93	2.5
94	1.5
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	1.7869230514078776E-4
7	0.0
8	0.0
9	0.0
10-11	0.005450115306794027
12-13	3.3504807213897704E-5
14-15	0.004154596094523315
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	107.0
20-21	514.0
22-23	1139.0
24-25	2706.0
26-27	3370.0
28-29	5816.0
30-31	13491.0
32-33	17232.0
34-35	24192.0
36-37	34960.0
38-39	41081.0
40-41	46225.0
42-43	56415.0
44-45	68599.0
46-47	67754.0
48-49	59603.0
50-51	50388.0
52-53	47047.0
54-55	50584.0
56-57	54599.0
58-59	56217.0
60-61	56954.0
62-63	57091.0
64-65	61039.0
66-67	64731.0
68-69	67851.0
70-71	67309.0
72-73	65066.0
74-75	66606.0
76-77	66168.0
78-79	71159.0
80-81	72582.0
82-83	70835.0
84-85	71887.0
86-87	75089.0
88-89	81281.0
90-91	84934.0
92-93	89960.0
94-95	109040.0
96-97	302796.0
98-99	560891.0
100-101	6088631.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.99946908880848
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.62574443379656	76.1814069356194
2	7.284248657606464	12.383145372148308
3	1.7384226478420854	4.432950063556138
4	0.625870270837208	2.1279456295852586
5	0.27703452933603917	1.1773893956415635
6	0.14718050696431423	0.7506158971313034
7	0.0904643766612059	0.5382596791356749
8	0.05777279380412599	0.39285254409023307
9	0.03478281446559886	0.2660868686691388
>10	0.11513952298268858	1.5051739029834335
>50	0.002698734423356448	0.15510991348724937
>100	6.189928644998873E-4	0.07948277864866504
>500	2.1718415838408864E-5	0.009581019303559412
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	1.1168269071299235E-5	0.0	0.0	0.0
4	0.0	5.584134535649618E-5	0.0	0.0	0.0
5	0.0	5.584134535649618E-5	0.0	0.0	0.0
6	0.0	5.584134535649618E-5	0.0	0.0	1.1168269071299235E-5
7	2.233653814259847E-5	5.584134535649618E-5	0.0	0.0	1.1168269071299235E-5
8	2.233653814259847E-5	5.584134535649618E-5	0.0	0.0	1.1168269071299235E-5
9	2.233653814259847E-5	1.1168269071299235E-4	0.0	0.0	1.1168269071299235E-5
10-11	3.3504807213897704E-5	1.1168269071299235E-4	0.0	0.0	1.1168269071299235E-5
12-13	4.467307628519694E-5	1.1726682524864196E-4	0.0	1.1168269071299235E-5	1.1168269071299235E-5
14-15	5.0257210820846556E-5	1.89860574212087E-4	0.0	1.1168269071299235E-5	1.6752403606948852E-5
16-17	5.584134535649618E-5	1.89860574212087E-4	0.0	1.1168269071299235E-5	2.233653814259847E-5
18-19	5.584134535649618E-5	1.89860574212087E-4	0.0	1.1168269071299235E-5	2.233653814259847E-5
20-21	5.584134535649618E-5	1.89860574212087E-4	0.0	1.6752403606948852E-5	2.233653814259847E-5
22-23	8.934615257039387E-5	1.9544470874773663E-4	0.0	4.467307628519694E-5	2.233653814259847E-5
24-25	1.2285095978429157E-4	2.959591303894297E-4	0.0	6.142547989214579E-5	2.233653814259847E-5
26-27	1.2285095978429157E-4	3.238798030676778E-4	0.0	1.3960336339124044E-4	2.233653814259847E-5
28-29	1.3401922885559082E-4	3.2946393760332744E-4	0.0	4.132259556380717E-4	2.233653814259847E-5
30-31	1.4518749792689006E-4	3.79721148424174E-4	0.0	0.0014518749792689005	2.233653814259847E-5
32-33	1.4518749792689006E-4	3.79721148424174E-4	0.0	0.004160180229058965	2.233653814259847E-5
34-35	1.5635576699818928E-4	4.5231489738761907E-4	0.0	0.008197509498333639	2.233653814259847E-5
36-37	1.89860574212087E-4	5.360769154223633E-4	0.0	0.014423819505582962	2.233653814259847E-5
38-39	1.89860574212087E-4	5.528293190293122E-4	0.0	0.032019427427414905	2.233653814259847E-5
40-41	1.89860574212087E-4	6.142547989214579E-4	0.0	0.0510389896558375	2.233653814259847E-5
42-43	1.9544470874773663E-4	6.310072025284067E-4	0.0	0.0619783092111751	2.233653814259847E-5
44-45	2.0102884328338622E-4	6.756802788136036E-4	0.0	0.07641329698582937	2.233653814259847E-5
46-47	2.1219711235468547E-4	7.091850860275014E-4	0.0	0.09144578715579814	2.233653814259847E-5
48-49	2.177812468903351E-4	7.14769220563151E-4	0.0	0.1067239792453355	2.233653814259847E-5
50-51	2.3453365049728393E-4	7.87362969526596E-4	0.0	0.12118130355813234	2.233653814259847E-5
52-53	2.4570191956858314E-4	8.041153731335449E-4	0.0	0.16143732942563044	2.792067267824809E-5
54-55	2.568701886398824E-4	8.152836422048442E-4	0.0	0.21209659793304378	3.3504807213897704E-5
56-57	2.792067267824809E-4	8.376201803474426E-4	0.0	0.2640402173836565	3.3504807213897704E-5
58-59	3.0154326492507936E-4	8.655408530256907E-4	0.0	0.302855536540957	3.3504807213897704E-5
60-61	3.0154326492507936E-4	8.767091220969899E-4	0.0	0.32983249048268026	3.3504807213897704E-5
62-63	3.0154326492507936E-4	8.822932566326395E-4	0.0	0.3474113460009053	3.3504807213897704E-5
64-65	3.0154326492507936E-4	9.325504674534862E-4	0.0	0.3524705718902038	3.3504807213897704E-5
66-67	3.0712739946072896E-4	9.437187365247854E-4	0.0	0.3569322953841879	3.3504807213897704E-5
68-69	3.1271153399637855E-4	9.772235437386831E-4	0.0	0.3616341366632049	3.3504807213897704E-5
70-71	3.1271153399637855E-4	0.0010163124854882305	0.0	0.36590599958297687	3.3504807213897704E-5
72-73	3.1271153399637855E-4	0.0010498172927021281	0.0	0.36934582645693703	3.3504807213897704E-5
74-75	3.1271153399637855E-4	0.001066569696309077	0.0	0.37236684324072344	3.3504807213897704E-5
76-77	3.1271153399637855E-4	0.0011112427725942738	0.0	0.37535993935183165	3.3504807213897704E-5
78-79	3.1829566853202814E-4	0.0011279951762012228	0.0	0.3774595739372359	3.3504807213897704E-5
80-81	3.238798030676778E-4	0.0011391634452725219	0.0	0.3781296700815139	3.3504807213897704E-5
82-83	3.238798030676778E-4	0.0011559158488794708	0.0	0.3785819849789015	3.3504807213897704E-5
84-85	3.238798030676778E-4	0.0011950047906290181	0.0	0.3787439248804353	3.3504807213897704E-5
86-87	3.238798030676778E-4	0.0012285095978429158	0.0	0.3788165186293988	4.467307628519694E-5
88	3.238798030676778E-4	0.0012955192122707112	0.0	0.3788276868984701	4.467307628519694E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	12530	0.0	51.08595	1
GTATCAA	22565	0.0	37.33743	1
TCAACGC	26995	0.0	30.537281	4
ATCAACG	27255	0.0	30.35705	3
CAACGCA	27605	0.0	29.866154	5
TATCAAC	28455	0.0	29.289633	2
AACGCAG	28300	0.0	29.270254	6
ACGCAGA	32240	0.0	25.639515	7
CGCAGAG	33205	0.0	24.89494	8
GTACATG	29095	0.0	21.584354	1
GCAGAGT	38570	0.0	21.332363	9
TACATGG	29335	0.0	21.06866	2
GAGTACT	21880	0.0	20.836178	12-13
ACATGGG	29120	0.0	20.64497	3
GTGGTAT	6075	0.0	19.43601	1
TGGTATC	5700	0.0	19.42477	2
CAGAGTA	38005	0.0	19.088438	10-11
AGAGTAC	34770	0.0	18.886227	10-11
GTACTTT	24445	0.0	18.570276	14-15
CATGGGG	20300	0.0	18.429386	4
>>END_MODULE