FastQCFastQC Report
Fri 27 May 2016
SRR2139527_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139527_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9386097
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA140050.050.328681
GTATCAA245050.036.0734561
TCAACGC298550.029.0455724
ATCAACG301700.028.7420083
CAACGCA303100.028.5550275
TATCAAC310900.028.099742
AACGCAG311550.027.9468126
ACGCAGA351950.024.7633537
CGCAGAG361150.024.144748
GTACATG314300.022.1926631
TACATGG318700.021.4664232
GCAGAGT414750.020.9941889
ACATGGG321200.020.6408793
GAGTACT237300.020.09120212-13
CTTATAC120350.019.2951721
CAGAGTA406500.019.15813610-11
CATGGGG215900.018.6728824
AGAGTAC383700.018.42327110-11
GTGGTAT70800.018.3504071
GTACTTT260400.018.20960414-15
Produced by FastQC (version 0.11.5)