##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139527_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9386097
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.61626211619164	33.0	33.0	33.0	33.0	33.0
2	32.624683507958636	33.0	33.0	33.0	33.0	33.0
3	32.62332724667133	33.0	33.0	33.0	33.0	33.0
4	36.53156194742074	37.0	37.0	37.0	37.0	37.0
5	36.55409399668467	37.0	37.0	37.0	37.0	37.0
6	36.539115033650305	37.0	37.0	37.0	37.0	37.0
7	36.50822221419617	37.0	37.0	37.0	37.0	37.0
8	36.50570647202986	37.0	37.0	37.0	37.0	37.0
9	36.5456161384226	37.0	37.0	37.0	37.0	37.0
10-11	36.468380840300284	37.0	37.0	37.0	37.0	37.0
12-13	36.46828026601472	37.0	37.0	37.0	37.0	37.0
14-15	38.52346028386453	40.0	37.0	40.0	37.0	40.0
16-17	38.45926677510364	40.0	37.0	40.0	37.0	40.0
18-19	38.48151579937859	40.0	37.0	40.0	37.0	40.0
20-21	38.47197800657967	40.0	37.0	40.0	37.0	40.0
22-23	38.47958301068807	40.0	37.0	40.0	37.0	40.0
24-25	38.46843735513309	40.0	37.0	40.0	37.0	40.0
26-27	38.36141030564407	40.0	37.0	40.0	37.0	40.0
28-29	38.29132052642257	40.0	37.0	40.0	37.0	40.0
30-31	38.237213507998135	40.0	37.0	40.0	37.0	40.0
32-33	38.17281661183205	40.0	37.0	40.0	37.0	40.0
34-35	38.13437037342651	40.0	37.0	40.0	37.0	40.0
36-37	38.07613770493016	40.0	37.0	40.0	33.0	40.0
38-39	38.02005278052248	40.0	37.0	40.0	33.0	40.0
40-41	38.00048768414502	40.0	37.0	40.0	33.0	40.0
42-43	37.94942693186228	40.0	37.0	40.0	33.0	40.0
44-45	37.885490154193	40.0	37.0	40.0	33.0	40.0
46-47	37.850368496902675	40.0	37.0	40.0	33.0	40.0
48-49	37.82929203354627	40.0	37.0	40.0	33.0	40.0
50-51	37.74936632828512	40.0	37.0	40.0	33.0	40.0
52-53	37.6527288273313	40.0	37.0	40.0	33.0	40.0
54-55	37.46171035459855	40.0	37.0	40.0	33.0	40.0
56-57	37.38502913900237	40.0	37.0	40.0	33.0	40.0
58-59	37.2716752608302	38.5	37.0	40.0	33.0	40.0
60-61	37.13687837080239	37.0	37.0	40.0	33.0	40.0
62-63	36.97849694172655	37.0	37.0	40.0	33.0	40.0
64-65	36.80402664925957	37.0	37.0	40.0	33.0	40.0
66-67	36.603148750535226	37.0	37.0	40.0	33.0	40.0
68-69	36.399474607259535	37.0	37.0	40.0	33.0	40.0
70-71	36.13232266891366	37.0	37.0	38.5	33.0	40.0
72-73	35.91405330374098	37.0	37.0	37.0	33.0	40.0
74-75	35.72306068676963	37.0	35.0	37.0	33.0	40.0
76-77	34.0872137188999	35.0	33.0	37.0	30.0	37.0
78-79	35.17630839643076	37.0	33.0	37.0	33.0	37.0
80-81	35.223561886332014	37.0	33.0	37.0	33.0	37.0
82-83	35.09796945037668	37.0	33.0	37.0	33.0	37.0
84-85	34.933255168572586	37.0	33.0	37.0	33.0	37.0
86-87	34.854632047619305	37.0	33.0	37.0	33.0	37.0
88-89	34.75137654065542	37.0	33.0	37.0	33.0	37.0
90-91	34.640291541331436	37.0	33.0	37.0	33.0	37.0
92-93	34.61283190501106	37.0	33.0	37.0	33.0	37.0
94-95	34.636178205303025	37.0	33.0	37.0	33.0	37.0
96-97	34.633451498632844	37.0	33.0	37.0	33.0	37.0
98-99	34.61922030569565	37.0	33.0	37.0	33.0	37.0
100	34.60440445520447	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	0.0
17	0.0
18	5.0
19	16.0
20	64.0
21	381.0
22	1293.0
23	3802.0
24	8924.0
25	17259.0
26	30061.0
27	47368.0
28	70323.0
29	98889.0
30	133638.0
31	178597.0
32	236098.0
33	317532.0
34	436193.0
35	635949.0
36	1111599.0
37	2680535.0
38	3299482.0
39	78088.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.961882239231066	20.734475682490817	14.767448067071967	23.53619401120615
2	14.36934862275555	21.23347968809613	42.0530706213669	22.344101067781423
3	17.449713123569893	27.121539442858943	30.9637115405903	24.465035892980865
4	12.008058301549623	18.8009670047092	39.51590314909381	29.675071544647363
5	12.834354897461639	38.27678320392385	35.963819679255394	12.925042219359122
6	28.78841391272293	38.75095953211589	19.2217202379906	13.238906317170576
7	25.80104382045061	33.15707263626191	23.54780693189086	17.494076611396622
8	24.25561977465181	36.66480327232928	21.445079887838364	17.634497065180554
9	25.494036552147286	16.945701711797778	21.345986516014058	36.214275220040875
10-11	23.779529085934556	26.972216749902163	29.23450760146987	20.013746562693413
12-13	24.892002455121638	24.58335965774045	29.084060854713456	21.44057703242446
14-15	22.050568337095257	26.001689802002296	26.763144842987458	25.18459701791499
16-17	21.409127776966294	29.126851128855797	27.438353769410224	22.025667324767685
18-19	21.420362478674576	28.1169478644851	29.23424401005018	21.22844564679014
20-21	22.275884435670974	27.308400327641376	29.162804478901904	21.252910757785745
22-23	22.065803161421833	27.31851222168461	28.963559291603342	21.652125325290214
24-25	21.82951347647206	27.531184707144305	29.09058258878496	21.548719227598674
26-27	21.78256258970456	27.70949490181178	29.003969788372125	21.50397272011153
28-29	21.543490753673915	27.50734014858628	29.154207327947002	21.794961769792803
30-31	22.001422370935636	27.488172351560046	29.034696499306428	21.475708778197884
32-33	21.916345540794683	27.556446643649863	29.040888913839357	21.486318901716103
34-35	21.70508783840877	27.64929105639009	29.17991891097294	21.4657021942282
36-37	21.873545835584842	27.572601938101194	28.882267624190543	21.671584602123417
38-39	21.6301886890213	27.692571074455056	28.997327372837024	21.679912863686624
40-41	21.82635466764694	27.539388648732317	29.02812136865853	21.606135314962213
42-43	21.850199511033807	27.574440597937546	28.757741401151886	21.817618489876757
44-45	21.807527556754362	27.84047537838531	28.537818209180333	21.814178855679994
46-47	22.050356361777336	27.675238479917365	28.069134173728315	22.205270984576984
48-49	21.93367660107119	27.81293005904028	28.113478157061007	22.13991518282752
50-51	21.91123280699259	27.95563601756229	28.02003085084638	22.11310032459874
52-53	22.024795960543695	27.93542310848924	28.029186590346526	22.01059434062054
54-55	22.084219858962797	27.861809385917574	27.917742403711486	22.136228351408143
56-57	22.12624814191215	28.033641627674072	27.701874794460572	22.1382354359532
58-59	21.847814990356877	28.088224008915596	27.97347214326158	22.090488857465946
60-61	22.211072322267544	28.238570619678594	27.749048391216526	21.801308666837336
62-63	21.8939526128962	28.321021103964906	27.786233393104276	21.998792890034622
64-65	21.91259754898352	28.098209708660267	27.779744847337096	22.209447895019117
66-67	22.040938624654814	28.117863110656042	27.741201880713078	22.099996383976066
68-69	21.973748372391047	28.187471990665724	27.74024666154536	22.098532975397866
70-71	21.9884201659518	27.953553585778877	27.654877859860772	22.403148388408546
72-73	22.061481529980195	27.98755019489737	27.541569267814264	22.40939900730817
74-75	21.99286983421162	28.145047747184176	27.66554892698405	22.19653349162015
76-77	22.076909729190735	27.887043042208663	27.632153371047114	22.403893857553488
78-79	22.07676711342234	27.830279862852443	27.403666567644436	22.689286456080783
80-81	22.167203490417712	28.14461962368223	27.38078438382972	22.307392502070336
82-83	22.322532704077673	27.88495062382111	27.489587888476013	22.3029287836252
84-85	22.18812576308253	27.873937960995836	27.387129447508613	22.55080682841302
86-87	22.01394067526978	28.122279217663955	27.51084803033802	22.352932076728248
88-89	22.302268552943872	28.013897377556635	27.507604992505673	22.176229076993824
90-91	22.361144259924842	28.104411223815973	27.42519064654989	22.109253869709296
92-93	22.234980335549437	27.89108297065707	27.46219075599396	22.411745937799537
94-95	22.180582168004335	28.01778358586385	27.589525776038077	22.212108470093735
96-97	22.437679951967716	27.997021221541967	27.643854916701383	21.921443909788938
98-99	23.26224031933505	29.992704783463616	27.90645969839256	18.83859519880878
100	22.22533619018854	30.97447103836209	21.791063900831904	25.00912887061747
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2330.0
1	2076.0
2	2788.0
3	3969.0
4	5307.5
5	8485.0
6	10976.5
7	12411.0
8	13168.0
9	12873.5
10	12030.5
11	11041.5
12	11970.0
13	15973.0
14	25643.0
15	39412.0
16	51168.0
17	58302.0
18	60018.5
19	56579.0
20	50029.5
21	42954.5
22	38269.0
23	38082.5
24	42980.5
25	52862.5
26	66967.5
27	83227.0
28	100789.0
29	122520.0
30	146141.5
31	169893.5
32	197251.0
33	225847.0
34	253443.0
35	279146.5
36	307579.0
37	332017.5
38	345323.0
39	355629.0
40	364891.0
41	369475.0
42	369875.5
43	375127.0
44	380618.0
45	381658.0
46	379712.5
47	374290.0
48	371491.0
49	371340.5
50	369370.5
51	356912.0
52	335966.0
53	316494.0
54	295823.0
55	270824.5
56	244111.0
57	217729.0
58	192527.0
59	166634.5
60	137578.0
61	109184.5
62	85258.5
63	64891.0
64	47453.0
65	34739.0
66	26239.0
67	20011.5
68	15119.0
69	10868.0
70	7591.5
71	5305.0
72	3601.0
73	2400.0
74	1552.0
75	1002.0
76	646.5
77	396.5
78	256.5
79	177.0
80	116.5
81	72.5
82	49.0
83	34.5
84	28.0
85	25.0
86	20.0
87	16.5
88	13.0
89	8.5
90	6.0
91	6.0
92	5.5
93	3.5
94	1.5
95	1.0
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	1.2784866808855696E-4
7	0.0
8	0.0
9	0.0
10-11	0.005092638612194185
12-13	4.261622269618564E-5
14-15	0.004069849267485729
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	122.0
20-21	608.0
22-23	1301.0
24-25	3071.0
26-27	3804.0
28-29	6456.0
30-31	14545.0
32-33	18814.0
34-35	25958.0
36-37	36953.0
38-39	43359.0
40-41	49150.0
42-43	60639.0
44-45	73567.0
46-47	72513.0
48-49	64207.0
50-51	54345.0
52-53	51787.0
54-55	58672.0
56-57	63681.0
58-59	42174.0
60-61	69988.0
62-63	70178.0
64-65	72267.0
66-67	77377.0
68-69	70368.0
70-71	69317.0
72-73	66261.0
74-75	68551.0
76-77	68044.0
78-79	73636.0
80-81	75670.0
82-83	73519.0
84-85	73463.0
86-87	77945.0
88-89	84657.0
90-91	88934.0
92-93	92722.0
94-95	112595.0
96-97	333212.0
98-99	584629.0
100-101	6337038.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.34057688910454
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.50453000881963	73.76018588234092
2	7.866859787112839	13.112572659273642
3	2.064205953359347	5.1609634491267595
4	0.7211207513875278	2.403944777093644
5	0.32906903712624735	1.3712401695221808
6	0.17590522217941382	0.8796025616543074
7	0.10675143375883961	0.6227708251240505
8	0.06098652957578302	0.40661220458481545
9	0.03679735055186434	0.27600411806846853
>10	0.13039024644598254	1.6797813133050228
>50	0.0025665426670247187	0.14678723663138768
>100	7.085970619147304E-4	0.10031230196180657
>500	6.512465668302597E-5	0.033361108026800826
>1k	4.341529682986145E-5	0.04586139328617177
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.065405567404641E-5
2	0.0	1.065405567404641E-5	0.0	1.065405567404641E-5	1.065405567404641E-5
3	0.0	1.065405567404641E-5	0.0	1.065405567404641E-5	1.065405567404641E-5
4	0.0	4.261622269618564E-5	0.0	2.130811134809282E-5	1.065405567404641E-5
5	0.0	4.261622269618564E-5	0.0	3.196216702213923E-5	1.065405567404641E-5
6	0.0	4.261622269618564E-5	0.0	3.196216702213923E-5	2.130811134809282E-5
7	0.0	4.261622269618564E-5	0.0	4.261622269618564E-5	2.130811134809282E-5
8	0.0	4.261622269618564E-5	0.0	5.3270278370232054E-5	2.130811134809282E-5
9	2.130811134809282E-5	1.1719461241451053E-4	0.0	1.1719461241451053E-4	2.130811134809282E-5
10-11	2.130811134809282E-5	1.1719461241451053E-4	0.0	1.2784866808855693E-4	2.130811134809282E-5
12-13	2.130811134809282E-5	1.1719461241451053E-4	0.0	1.2784866808855693E-4	2.130811134809282E-5
14-15	2.130811134809282E-5	1.4915677943664978E-4	0.0	1.3850272376260335E-4	3.196216702213923E-5
16-17	2.130811134809282E-5	1.6513786294771937E-4	0.0	1.3850272376260335E-4	4.794325053320885E-5
18-19	2.130811134809282E-5	1.917730021328354E-4	0.0	1.3850272376260335E-4	6.925136188130166E-5
20-21	2.6635139185116027E-5	2.1308111348092822E-4	0.0	1.5448380727367295E-4	9.58865010664177E-5
22-23	3.196216702213923E-5	2.5037030834009063E-4	0.0	1.5981083511069617E-4	1.1719461241451052E-4
24-25	3.196216702213923E-5	2.716784196881835E-4	0.0	1.7046489078474257E-4	1.4915677943664975E-4
26-27	3.196216702213923E-5	2.876595031992531E-4	0.0	2.2906219699199784E-4	1.4915677943664975E-4
28-29	3.196216702213923E-5	2.876595031992531E-4	0.0	4.368162826359029E-4	1.5448380727367295E-4
30-31	4.261622269618564E-5	3.782189764286476E-4	0.0	0.0014382975159962655	1.5981083511069617E-4
32-33	4.261622269618564E-5	3.9420005993971724E-4	0.0	0.0038407870704937315	1.5981083511069617E-4
34-35	1.1719461241451053E-4	4.634514218210189E-4	0.0	0.006845230770574819	1.5981083511069617E-4
36-37	1.1719461241451053E-4	5.327027837023206E-4	0.0	0.011943196410606028	1.5981083511069617E-4
38-39	1.3850272376260333E-4	5.593379228874366E-4	0.0	0.02779110422575006	1.6513786294771937E-4
40-41	1.4915677943664975E-4	6.285892847687382E-4	0.0	0.04535431500441557	1.7046489078474257E-4
42-43	1.4915677943664975E-4	6.445703682798078E-4	0.0	0.05570473009175166	1.757919186217658E-4
44-45	1.4915677943664975E-4	7.138217301611096E-4	0.0	0.06861744556869592	1.81118946458789E-4
46-47	1.7046489078474257E-4	7.564379528572952E-4	0.0	0.08233986927686769	1.971000299698586E-4
48-49	1.81118946458789E-4	7.564379528572952E-4	0.0	0.09576397942616617	2.0242705780688182E-4
50-51	1.81118946458789E-4	8.363433704126433E-4	0.0	0.1083304380937039	2.0242705780688182E-4
52-53	1.81118946458789E-4	8.416703982496665E-4	0.0	0.14507627611349	2.1308111348092822E-4
54-55	1.9177300213283542E-4	8.469974260866897E-4	0.0	0.18978069372178874	2.2373516915497464E-4
56-57	2.3971625266604426E-4	8.523244539237129E-4	0.0	0.23854430654189915	2.3971625266604426E-4
58-59	2.770054475252067E-4	8.523244539237129E-4	0.0	0.4054454157036732	2.4504328050306746E-4
60-61	2.770054475252067E-4	8.736325652718058E-4	0.0	0.5480606049564585	2.4504328050306746E-4
62-63	2.770054475252067E-4	8.842866209458521E-4	0.0	0.5544477113330493	2.4504328050306746E-4
64-65	2.823324753622299E-4	9.908271776863163E-4	0.0	0.5593911931658069	2.4504328050306746E-4
66-67	2.9298653103627633E-4	0.0010014812333603627	0.0	0.5643400020264013	2.4504328050306746E-4
68-69	2.983135588732995E-4	0.001012135289034409	0.0	0.5689745162446116	2.4504328050306746E-4
70-71	2.983135588732995E-4	0.0010227893447084554	0.0	0.5730656736234454	2.4504328050306746E-4
72-73	2.983135588732995E-4	0.0010547515117305946	0.0	0.5763045065483555	2.5569733617711386E-4
74-75	3.0896761454734595E-4	0.0010973677344267804	0.0	0.578952039383356	2.5569733617711386E-4
76-77	3.0896761454734595E-4	0.0011453109849599893	0.0	0.58145041543892	2.610243640141371E-4
78-79	3.0896761454734595E-4	0.0011719461241451053	0.0	0.5831710454302784	2.663513918511603E-4
80-81	3.249486980584156E-4	0.0011719461241451053	0.0	0.5836824401026326	2.823324753622299E-4
82-83	3.4092978156948514E-4	0.0011772731519821287	0.0	0.5841405644966167	2.983135588732995E-4
84-85	3.515838372435316E-4	0.0011826001798191518	0.0	0.5843216834430753	2.983135588732995E-4
86-87	3.62237892917578E-4	0.001193254235493198	0.0	0.584428223999816	3.036405867103227E-4
88	3.62237892917578E-4	0.001267832625211523	0.0	0.58443887805549	3.0896761454734595E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	14005	0.0	50.32868	1
GTATCAA	24505	0.0	36.073456	1
TCAACGC	29855	0.0	29.045572	4
ATCAACG	30170	0.0	28.742008	3
CAACGCA	30310	0.0	28.555027	5
TATCAAC	31090	0.0	28.09974	2
AACGCAG	31155	0.0	27.946812	6
ACGCAGA	35195	0.0	24.763353	7
CGCAGAG	36115	0.0	24.14474	8
GTACATG	31430	0.0	22.192663	1
TACATGG	31870	0.0	21.466423	2
GCAGAGT	41475	0.0	20.994188	9
ACATGGG	32120	0.0	20.640879	3
GAGTACT	23730	0.0	20.091202	12-13
CTTATAC	12035	0.0	19.295172	1
CAGAGTA	40650	0.0	19.158136	10-11
CATGGGG	21590	0.0	18.672882	4
AGAGTAC	38370	0.0	18.423271	10-11
GTGGTAT	7080	0.0	18.350407	1
GTACTTT	26040	0.0	18.209604	14-15
>>END_MODULE