FastQCFastQC Report
Fri 27 May 2016
SRR2139526_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139526_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10522312
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA137300.050.8455921
GTATCAA243600.037.90491
TCAACGC284650.031.6228834
ATCAACG289900.031.1557253
CAACGCA292600.030.7817335
TATCAAC301050.030.3657342
AACGCAG300850.029.9667636
ACGCAGA341000.026.4641257
CGCAGAG350100.025.751468
GCAGAGT414150.021.7180429
GTACATG330550.021.4510941
ATCTCGT116950.021.05535594
GAGTACT223950.020.96981412-13
TACATGG333100.020.5764372
ACATGGG335300.020.036263
CAGAGTA402250.019.63075810-11
GTGGTAT68800.019.0454311
AGAGTAC372150.018.77453410-11
GTACTTT253700.018.58889414-15
TATCTCG130150.018.33923194
Produced by FastQC (version 0.11.5)