##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139526_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10522312
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.64322945375503	33.0	33.0	33.0	33.0	33.0
2	32.649282115945624	33.0	33.0	33.0	33.0	33.0
3	32.65090561846104	33.0	33.0	33.0	33.0	33.0
4	36.56321376898917	37.0	37.0	37.0	37.0	37.0
5	36.5837488947296	37.0	37.0	37.0	37.0	37.0
6	36.567769516813414	37.0	37.0	37.0	37.0	37.0
7	36.534617106962806	37.0	37.0	37.0	37.0	37.0
8	36.53280695345281	37.0	37.0	37.0	37.0	37.0
9	36.57097774709589	37.0	37.0	37.0	37.0	37.0
10-11	36.4965906257104	37.0	37.0	37.0	37.0	37.0
12-13	36.49575050616252	37.0	37.0	37.0	37.0	37.0
14-15	38.59712181125213	40.0	37.0	40.0	37.0	40.0
16-17	38.53635565073532	40.0	37.0	40.0	37.0	40.0
18-19	38.55113282137994	40.0	37.0	40.0	37.0	40.0
20-21	38.53706204747422	40.0	37.0	40.0	37.0	40.0
22-23	38.5447031008227	40.0	37.0	40.0	37.0	40.0
24-25	38.53168146569658	40.0	37.0	40.0	37.0	40.0
26-27	38.434067959443176	40.0	37.0	40.0	37.0	40.0
28-29	38.37671131894135	40.0	37.0	40.0	37.0	40.0
30-31	38.325357941647056	40.0	37.0	40.0	37.0	40.0
32-33	38.26186657346888	40.0	37.0	40.0	37.0	40.0
34-35	38.230045960405796	40.0	37.0	40.0	37.0	40.0
36-37	38.17215307046611	40.0	37.0	40.0	37.0	40.0
38-39	38.123680596060844	40.0	37.0	40.0	37.0	40.0
40-41	38.095105718681594	40.0	37.0	40.0	35.0	40.0
42-43	38.03810583074827	40.0	37.0	40.0	33.0	40.0
44-45	37.97431876138298	40.0	37.0	40.0	33.0	40.0
46-47	37.93157930665271	40.0	37.0	40.0	33.0	40.0
48-49	37.90679921005355	40.0	37.0	40.0	33.0	40.0
50-51	37.82499228664784	40.0	37.0	40.0	33.0	40.0
52-53	37.72059544871685	40.0	37.0	40.0	33.0	40.0
54-55	37.522857641717266	40.0	37.0	40.0	33.0	40.0
56-57	37.43859180881181	40.0	37.0	40.0	33.0	40.0
58-59	37.32010356406924	38.5	37.0	40.0	33.0	40.0
60-61	37.17820137047296	37.0	37.0	40.0	33.0	40.0
62-63	37.01778121563307	37.0	37.0	40.0	33.0	40.0
64-65	36.83527181364181	37.0	37.0	40.0	33.0	40.0
66-67	36.62336916892503	37.0	37.0	40.0	33.0	40.0
68-69	36.412435660100606	37.0	37.0	40.0	33.0	40.0
70-71	36.14339881478833	37.0	37.0	38.5	33.0	40.0
72-73	35.927392167546884	37.0	37.0	37.0	33.0	40.0
74-75	35.727498990699026	37.0	35.0	37.0	33.0	40.0
76-77	34.080528059491925	35.0	33.0	37.0	30.0	37.0
78-79	35.16504858556921	37.0	33.0	37.0	33.0	37.0
80-81	35.212448746119136	37.0	33.0	37.0	33.0	37.0
82-83	35.09133459782278	37.0	33.0	37.0	33.0	37.0
84-85	34.931267901776984	37.0	33.0	37.0	33.0	37.0
86-87	34.8519602073968	37.0	33.0	37.0	33.0	37.0
88-89	34.75215864176315	37.0	33.0	37.0	33.0	37.0
90-91	34.65067088367629	37.0	33.0	37.0	33.0	37.0
92-93	34.634798356036114	37.0	33.0	37.0	33.0	37.0
94-95	34.650658822109605	37.0	33.0	37.0	33.0	37.0
96-97	34.63832265881097	37.0	33.0	37.0	33.0	37.0
98-99	34.622009903797796	37.0	33.0	37.0	33.0	37.0
100	34.60078728990767	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	7.0
20	74.0
21	350.0
22	1433.0
23	4095.0
24	9367.0
25	18489.0
26	32734.0
27	51755.0
28	76563.0
29	107134.0
30	146261.0
31	195065.0
32	256738.0
33	344164.0
34	474754.0
35	694810.0
36	1226963.0
37	3049003.0
38	3744588.0
39	87963.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.440578838567035	20.241254963738005	14.492309294763356	23.8258569029316
2	14.482282981154713	21.107262358310606	41.60872629513362	22.801728365401065
3	17.677464800511522	26.609513194438637	30.73608727815712	24.976934726892722
4	12.272730555794203	18.451230109884595	39.12631558539606	30.14972374892514
5	13.221733018370868	38.01261547842337	35.675258441300734	13.090393061905026
6	29.604186309474763	38.14653052531581	19.046911706272553	13.202371458936874
7	26.43598669189813	32.781426743476146	23.250641113854066	17.531945450771655
8	24.612927273017565	36.457405938922925	21.243487172781037	17.686179615278466
9	25.81877442904183	16.773528479292384	20.974800975298965	36.43289611636682
10-11	24.143075540376024	26.65026765219789	29.10438884786548	20.102267959560606
12-13	25.177780566560877	24.285048964764062	28.926757205391613	21.61041326328345
14-15	22.287658998726556	25.637408629519054	26.471220252767075	25.603712118987314
16-17	21.804936975828127	28.822852810294926	27.117001472680148	22.255208741196803
18-19	21.934670821393627	27.936588460786943	28.759430436961004	21.36931028085843
20-21	22.715597692201612	27.063438034917674	28.663062557219053	21.557901715661654
22-23	22.45954974002626	27.092837308857163	28.450146869361113	21.997466081755455
24-25	22.26821445844122	27.263975815852547	28.52806808445914	21.93974164124709
26-27	22.14929110353563	27.423557631984323	28.522705288330492	21.904445976149553
28-29	22.080499854430773	27.32088742197682	28.59293083865902	22.005681884933388
30-31	22.264129209358316	27.216359702494607	28.532324354273314	21.98718673387376
32-33	22.044905346825217	27.4432193442993	28.443032112372713	22.068843196502765
34-35	22.25262031542155	27.39268468178685	28.437955259091908	21.916739743699694
36-37	22.18853109232368	27.360428068323213	28.443942019232605	22.0070988201205
38-39	22.083182775603476	27.42012197156878	28.425675541029456	22.071019711798293
40-41	22.337740366660263	27.262438434648136	28.42199171280223	21.977829485889373
42-43	22.219291887206385	27.335826286125087	28.219866990334463	22.225014836334065
44-45	22.236701597367784	27.50021989363869	28.036662966219566	22.226415542773964
46-47	22.38994563191812	27.386224450963976	27.814925289985336	22.408904627132568
48-49	22.289875123183847	27.557649152358383	27.754832166125798	22.397643558331968
50-51	22.240816219310823	27.67085043187924	27.64869322905776	22.439640119752184
52-53	22.3497043090099	27.536514017012248	27.62170965688297	22.492072017094877
54-55	22.42422517650664	27.379703408841593	27.699757347918307	22.496314066733458
56-57	22.34236110147562	27.31101085322606	27.700282383319607	22.64634566197871
58-59	22.132568603660633	27.235627782245963	27.86895577774539	22.76284783634801
60-61	22.35393338783312	27.449447227230433	27.885395253613478	22.311224131322973
62-63	21.990947171045363	27.675950450113245	27.86015728289405	22.472945095947345
64-65	21.936837786185013	27.785426383843397	27.66542733416939	22.6123084958022
66-67	22.00322333537359	28.110063084896293	27.335056631904877	22.551656947825247
68-69	22.0197266605231	28.11660735260221	27.19026450440906	22.67340148246563
70-71	22.02291132066051	27.704430613914926	27.212930567849526	23.05972749757504
72-73	22.20198089605808	27.417760721342745	27.15357351067178	23.226684871927404
74-75	22.237874177907557	27.499165007957295	27.25115844368625	23.011802370448894
76-77	22.50352022178534	27.351833096155744	26.892943269135365	23.251703412923554
78-79	22.60943827118744	27.399984229312274	26.552444602223236	23.438132897277054
80-81	22.485651274918258	27.68727466551842	26.648260490048536	23.17881356951479
82-83	22.539795982203987	27.489201825123477	26.722109719969446	23.24889247270309
84-85	22.562567175473212	27.771207384539387	26.66895178519989	22.997273654787506
86-87	22.795500513339842	27.707852281215118	26.7752354789167	22.72141172652834
88-89	22.69400030747944	27.74551441762038	26.966155783552566	22.59432949134762
90-91	22.652610519342442	27.581127754596984	26.86364840643074	22.90261331962983
92-93	22.637801910756032	27.27464465655795	27.074087253946583	23.013466178739435
94-95	22.143254060036128	27.520243821747798	27.488653564077865	22.847848554138206
96-97	22.294861479167103	27.515341480235644	27.53860249988283	22.651194540714418
98-99	23.482115518396103	29.345000889420565	27.898948863663982	19.27393472851936
100	22.44060687985166	30.450777436194485	21.6181212507574	25.490494433196453
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2228.0
1	1962.0
2	2499.5
3	3511.0
4	4694.0
5	7446.0
6	9752.5
7	11254.0
8	12125.5
9	12054.5
10	11436.5
11	10655.5
12	11594.0
13	15274.0
14	24117.0
15	36470.0
16	46774.5
17	53126.5
18	54854.0
19	51673.0
20	45431.5
21	38911.5
22	34608.0
23	34782.5
24	39702.0
25	49155.5
26	62578.0
27	78358.0
28	96451.0
29	118025.5
30	141269.0
31	165094.5
32	192490.0
33	221073.0
34	248710.5
35	275218.0
36	303485.5
37	329115.0
38	348768.0
39	366450.0
40	382859.5
41	396015.5
42	404100.5
43	413347.0
44	422862.5
45	430448.0
46	434424.5
47	435144.0
48	439802.5
49	438795.5
50	429140.5
51	415607.0
52	397099.0
53	374442.0
54	349312.0
55	322031.5
56	292641.0
57	262865.5
58	231208.0
59	198320.5
60	164817.5
61	132636.5
62	103905.5
63	79163.0
64	58085.0
65	42384.5
66	31743.5
67	24167.0
68	18478.0
69	13532.0
70	9482.0
71	6675.0
72	4612.0
73	3110.5
74	2039.0
75	1289.0
76	833.5
77	520.5
78	337.0
79	217.0
80	144.5
81	103.5
82	75.0
83	56.5
84	40.5
85	28.0
86	21.5
87	18.0
88	14.5
89	13.5
90	12.0
91	8.5
92	6.5
93	3.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	1.2354699233400418E-4
7	0.0
8	0.0
9	0.0
10-11	0.005146207411451019
12-13	4.751807397461699E-6
14-15	0.004286130272510452
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	138.0
20-21	624.0
22-23	1348.0
24-25	3081.0
26-27	3666.0
28-29	6027.0
30-31	13758.0
32-33	17513.0
34-35	24808.0
36-37	35326.0
38-39	28279.0
40-41	47613.0
42-43	57073.0
44-45	69752.0
46-47	68932.0
48-49	61362.0
50-51	34671.0
52-53	12946.0
54-55	13681.0
56-57	14302.0
58-59	40948.0
60-61	70818.0
62-63	70455.0
64-65	74361.0
66-67	80993.0
68-69	77184.0
70-71	76555.0
72-73	73759.0
74-75	76184.0
76-77	75447.0
78-79	82194.0
80-81	84497.0
82-83	82803.0
84-85	82959.0
86-87	88570.0
88-89	95728.0
90-91	99787.0
92-93	105680.0
94-95	128502.0
96-97	372797.0
98-99	719707.0
100-101	7347484.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	82.47186383615328
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.96335831639894	72.54502109640814
2	8.178583667575154	13.490060772096903
3	2.1204711992762357	5.24637636045583
4	0.8022452249737928	2.646506358289712
5	0.3584427405872485	1.4780720447384585
6	0.18562775837079679	0.918544032754003
7	0.1156399650684378	0.6675930417199206
8	0.07477270801126194	0.4933315675012194
9	0.04644348137401844	0.34472524247594727
>10	0.15047365726799863	1.8673668707126747
>50	0.003181707388746644	0.17493374669830847
>100	6.984118451333294E-4	0.09269843877046102
>500	6.116186220662949E-5	0.03477042737836322
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.9007229589846794E-5	0.0	0.0	0.0
3	0.0	1.9007229589846794E-5	0.0	2.851084438477019E-5	0.0
4	0.0	2.851084438477019E-5	0.0	4.751807397461699E-5	0.0
5	0.0	2.851084438477019E-5	0.0	4.751807397461699E-5	0.0
6	3.801445917969359E-5	2.851084438477019E-5	0.0	4.751807397461699E-5	9.503614794923397E-6
7	3.801445917969359E-5	3.801445917969359E-5	0.0	4.751807397461699E-5	9.503614794923397E-6
8	3.801445917969359E-5	3.801445917969359E-5	0.0	4.751807397461699E-5	9.503614794923397E-6
9	3.801445917969359E-5	5.702168876954038E-5	0.0	6.652530356446379E-5	9.503614794923397E-6
10-11	3.801445917969359E-5	6.652530356446379E-5	0.0	9.503614794923398E-5	9.503614794923397E-6
12-13	3.801445917969359E-5	6.652530356446379E-5	0.0	1.0453976274415737E-4	9.503614794923397E-6
14-15	4.751807397461699E-5	9.503614794923398E-5	0.0	1.2829879973146586E-4	2.3759036987308494E-5
16-17	4.751807397461699E-5	9.503614794923398E-5	0.0	1.4730602932131267E-4	2.851084438477019E-5
18-19	4.751807397461699E-5	9.503614794923398E-5	0.0	1.7106506630862114E-4	4.276626657715529E-5
20-21	4.751807397461699E-5	1.0929157014161906E-4	0.0	1.8056868110354453E-4	5.2269881372078684E-5
22-23	4.751807397461699E-5	1.5205783671877436E-4	0.0	1.9957591069339134E-4	5.702168876954038E-5
24-25	4.751807397461699E-5	1.8056868110354453E-4	0.0	2.375903698730849E-4	5.702168876954038E-5
26-27	4.751807397461699E-5	1.9957591069339134E-4	0.0	3.6113736220708906E-4	5.702168876954038E-5
28-29	4.751807397461699E-5	1.9957591069339134E-4	0.0	9.075952129151844E-4	5.702168876954038E-5
30-31	4.751807397461699E-5	2.470939846680083E-4	0.0	0.0030173976973881787	5.702168876954038E-5
32-33	4.751807397461699E-5	2.470939846680083E-4	0.0	0.007983036427735653	5.702168876954038E-5
34-35	4.751807397461699E-5	2.8986025124516364E-4	0.0	0.01502046318337643	5.702168876954038E-5
36-37	5.2269881372078684E-5	3.326265178223189E-4	0.0	0.02805942268201133	5.702168876954038E-5
38-39	5.702168876954038E-5	3.706409770020125E-4	0.0	0.11737439452470141	5.702168876954038E-5
40-41	5.702168876954038E-5	4.276626657715529E-4	0.0	0.2134369328717871	5.702168876954038E-5
42-43	5.702168876954038E-5	4.371662805664763E-4	0.0	0.24270331463275369	5.702168876954038E-5
44-45	5.702168876954038E-5	4.751807397461699E-4	0.0	0.28246168712731573	5.702168876954038E-5
46-47	5.702168876954038E-5	5.0369158413094E-4	0.0	0.32375014160386045	6.177349616700209E-5
48-49	5.702168876954038E-5	5.084433915284017E-4	0.0	0.36549952139795894	8.553253315431057E-5
50-51	6.652530356446379E-5	5.98727732080174E-4	0.0	0.4043122842204261	8.553253315431057E-5
52-53	6.652530356446379E-5	6.129831542725591E-4	0.0	0.7273306474850775	9.028434055177227E-5
54-55	6.652530356446379E-5	6.177349616700208E-4	0.0	1.1670961666979651	9.978795534669567E-5
56-57	6.652530356446379E-5	6.367421912598676E-4	0.0	1.665698565106224	1.0453976274415737E-4
58-59	6.652530356446379E-5	6.46245806054791E-4	0.0	2.1891956824697845	1.0929157014161906E-4
60-61	6.652530356446379E-5	6.605012282471761E-4	0.0	2.3381268299210287	1.1404337753908076E-4
62-63	8.078072575684887E-5	6.652530356446378E-4	0.0	2.344570280751987	1.1404337753908076E-4
64-65	8.553253315431057E-5	7.127711096192548E-4	0.0	2.349958830340708	1.1404337753908076E-4
66-67	8.553253315431057E-5	7.127711096192548E-4	0.0	2.354083399161705	1.1879518493654246E-4
68-69	8.553253315431057E-5	7.270265318116398E-4	0.0	2.35845506196737	1.2354699233400415E-4
70-71	8.553253315431057E-5	7.317783392091015E-4	0.0	2.3624133175294553	1.2354699233400415E-4
72-73	9.503614794923398E-5	7.460337614014867E-4	0.0	2.3653451826936895	1.4255422192385096E-4
74-75	9.503614794923398E-5	7.697927983887952E-4	0.0	2.3677876116959844	1.5205783671877436E-4
76-77	9.503614794923398E-5	8.220626797608738E-4	0.0	2.370101741898549	1.6156145151369775E-4
78-79	9.503614794923398E-5	8.363181019532589E-4	0.0	2.3717363636432753	1.6156145151369775E-4
80-81	9.503614794923398E-5	8.410699093507207E-4	0.0	2.3723636022197403	1.7581687370608285E-4
82-83	9.978795534669567E-5	8.458217167481824E-4	0.0	2.372886301033461	1.9007229589846795E-4
84-85	1.0453976274415737E-4	8.790843685304142E-4	0.0	2.373109635981142	1.9007229589846795E-4
86-87	1.0929157014161906E-4	8.933397907227994E-4	0.0	2.373171409477309	1.9007229589846795E-4
88	1.1404337753908076E-4	9.218506351075695E-4	0.0	2.3731761612847064	1.9007229589846795E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	13730	0.0	50.845592	1
GTATCAA	24360	0.0	37.9049	1
TCAACGC	28465	0.0	31.622883	4
ATCAACG	28990	0.0	31.155725	3
CAACGCA	29260	0.0	30.781733	5
TATCAAC	30105	0.0	30.365734	2
AACGCAG	30085	0.0	29.966763	6
ACGCAGA	34100	0.0	26.464125	7
CGCAGAG	35010	0.0	25.75146	8
GCAGAGT	41415	0.0	21.718042	9
GTACATG	33055	0.0	21.451094	1
ATCTCGT	11695	0.0	21.055355	94
GAGTACT	22395	0.0	20.969814	12-13
TACATGG	33310	0.0	20.576437	2
ACATGGG	33530	0.0	20.03626	3
CAGAGTA	40225	0.0	19.630758	10-11
GTGGTAT	6880	0.0	19.045431	1
AGAGTAC	37215	0.0	18.774534	10-11
GTACTTT	25370	0.0	18.588894	14-15
TATCTCG	13015	0.0	18.339231	94
>>END_MODULE