FastQCFastQC Report
Fri 27 May 2016
SRR2139525_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139525_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10165201
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA139150.050.5350461
GTATCAA244700.037.4311831
TCAACGC293150.030.6500874
ATCAACG296150.030.4424973
CAACGCA300300.029.9087455
TATCAAC308900.029.3976672
AACGCAG309250.029.0854576
ACGCAGA350300.025.6646217
CGCAGAG359000.025.00668
GTACATG303800.021.9555911
GCAGAGT414100.021.5120379
GAGTACT242100.021.19606412-13
TACATGG309500.020.9032962
ACATGGG311300.020.138233
CAGAGTA406000.019.45492710-11
AGAGTAC375700.018.91169710-11
GTACTTT271850.018.86056514-15
GTGGTAT65450.018.7836911
CATGGGG226600.017.4498214
TGGTATC65800.017.4249422
Produced by FastQC (version 0.11.5)