FastQCFastQC Report
Fri 27 May 2016
SRR2139524_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139524_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8731839
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA116700.052.6297531
GTATCAA217750.038.128331
TCAACGC259600.031.2603424
ATCAACG264950.030.6947043
CAACGCA267300.030.362625
TATCAAC272300.029.9621662
AACGCAG273000.029.6648456
ACGCAGA312950.025.9197067
CGCAGAG322950.025.0907088
GTACATG280350.022.6537481
TACATGG285400.021.8102862
GCAGAGT372250.021.7109749
GTGGTAT55650.021.0557251
ACATGGG288050.020.9140133
GAGTACT209950.020.63558412-13
AGAGTAC336050.019.25917410-11
CAGAGTA365750.019.20809410-11
CATGGGG212500.019.0433064
TGGTATC57150.018.9786872
GTACTTT233200.018.67194414-15
Produced by FastQC (version 0.11.5)