##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139524_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8731839
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.628617637132336	33.0	33.0	33.0	33.0	33.0
2	32.63205322498502	33.0	33.0	33.0	33.0	33.0
3	32.63031143840375	33.0	33.0	33.0	33.0	33.0
4	36.54031916987933	37.0	37.0	37.0	37.0	37.0
5	36.56193729637022	37.0	37.0	37.0	37.0	37.0
6	36.547072959086854	37.0	37.0	37.0	37.0	37.0
7	36.513312831352025	37.0	37.0	37.0	37.0	37.0
8	36.51050620608099	37.0	37.0	37.0	37.0	37.0
9	36.55174036076478	37.0	37.0	37.0	37.0	37.0
10-11	36.47500835734603	37.0	37.0	37.0	37.0	37.0
12-13	36.47451630750406	37.0	37.0	37.0	37.0	37.0
14-15	38.54715404166293	40.0	37.0	40.0	37.0	40.0
16-17	38.48624035555397	40.0	37.0	40.0	37.0	40.0
18-19	38.50444413828519	40.0	37.0	40.0	37.0	40.0
20-21	38.48928316583816	40.0	37.0	40.0	37.0	40.0
22-23	38.49819226722225	40.0	37.0	40.0	37.0	40.0
24-25	38.48585730719158	40.0	37.0	40.0	37.0	40.0
26-27	38.383342340334735	40.0	37.0	40.0	37.0	40.0
28-29	38.32064486216129	40.0	37.0	40.0	37.0	40.0
30-31	38.26796253603315	40.0	37.0	40.0	37.0	40.0
32-33	38.20265376120616	40.0	37.0	40.0	37.0	40.0
34-35	38.16437130754966	40.0	37.0	40.0	37.0	40.0
36-37	38.10721464397216	40.0	37.0	40.0	35.0	40.0
38-39	38.05597644403451	40.0	37.0	40.0	33.0	40.0
40-41	38.02598607602053	40.0	37.0	40.0	33.0	40.0
42-43	37.969234564563294	40.0	37.0	40.0	33.0	40.0
44-45	37.8996178153889	40.0	37.0	40.0	33.0	40.0
46-47	37.85748632174159	40.0	37.0	40.0	33.0	40.0
48-49	37.834223465035	40.0	37.0	40.0	33.0	40.0
50-51	37.751691755423565	40.0	37.0	40.0	33.0	40.0
52-53	37.643306535159724	40.0	37.0	40.0	33.0	40.0
54-55	37.44329731665205	40.0	37.0	40.0	33.0	40.0
56-57	37.357310152402945	40.0	37.0	40.0	33.0	40.0
58-59	37.23656688630044	37.0	37.0	40.0	33.0	40.0
60-61	37.09134653603755	37.0	37.0	40.0	33.0	40.0
62-63	36.923059782507394	37.0	37.0	40.0	33.0	40.0
64-65	36.734602117222195	37.0	37.0	40.0	33.0	40.0
66-67	36.52646202474448	37.0	37.0	40.0	33.0	40.0
68-69	36.32107358883255	37.0	37.0	40.0	33.0	40.0
70-71	36.05262907295888	37.0	37.0	38.5	33.0	40.0
72-73	35.835161993410466	37.0	37.0	37.0	33.0	40.0
74-75	35.647168918871685	37.0	33.0	37.0	33.0	40.0
76-77	34.00624814662653	35.0	33.0	37.0	27.0	37.0
78-79	35.1168113983209	37.0	33.0	37.0	33.0	37.0
80-81	35.18039337857887	37.0	33.0	37.0	33.0	37.0
82-83	35.05976775452365	37.0	33.0	37.0	33.0	37.0
84-85	34.89613089306166	37.0	33.0	37.0	33.0	37.0
86-87	34.819326756576245	37.0	33.0	37.0	33.0	37.0
88-89	34.71978173297446	37.0	33.0	37.0	33.0	37.0
90-91	34.60952439024448	37.0	33.0	37.0	33.0	37.0
92-93	34.584210751372105	37.0	33.0	37.0	33.0	37.0
94-95	34.61283748928223	37.0	33.0	37.0	33.0	37.0
96-97	34.60758037107751	37.0	33.0	37.0	33.0	37.0
98-99	34.58436316488424	37.0	33.0	37.0	33.0	37.0
100	34.56784682299041	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	6.0
20	58.0
21	351.0
22	1223.0
23	3633.0
24	8329.0
25	16204.0
26	28507.0
27	44972.0
28	66058.0
29	92380.0
30	125152.0
31	166444.0
32	220044.0
33	295342.0
34	406294.0
35	593401.0
36	1046428.0
37	2544134.0
38	3004973.0
39	67906.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.499448168936695	20.279370703009985	14.688074299125303	23.53310682892802
2	14.633687130511683	21.01902016287749	41.52558241167754	22.82171029493329
3	17.834547796861578	26.416428429337735	30.67873789244167	25.070285881359013
4	12.388146414518179	18.321020348634462	38.82203966426774	30.46879357257961
5	13.333674613102692	37.91043330047656	35.50712513137267	13.248766955048072
6	29.899120825961838	37.892666739350915	18.859765052740364	13.348447381946881
7	26.540251142972288	32.82735744440547	23.02733708214272	17.605054330479526
8	24.7750216191572	36.42643892082756	21.005964493848317	17.79257496616692
9	25.834168495319254	16.734332824963904	20.936105212201003	36.49539346751583
10-11	24.25772108197652	26.66302385632286	28.849763736529294	20.22949132517133
12-13	25.248170817634797	24.23799409482894	28.839991715375863	21.6738433721604
14-15	22.34578138817695	25.61186181736596	26.443625679576844	25.598731114880245
16-17	21.83218792742285	28.79666585698614	27.100997853945774	22.27014836164524
18-19	21.88632887069952	27.8104303114155	28.868598012400366	21.434642805484618
20-21	22.69318707858593	26.920166191496786	28.845153942902197	21.541492787015095
22-23	22.409182997307695	26.919871943729735	28.69871631623643	21.972228742726138
24-25	22.171669114198238	27.12744372028159	28.76406996539968	21.936817200120487
26-27	22.059029784628304	27.23303928978537	28.744832733894558	21.963098191691767
28-29	21.94702411425435	27.157251505521717	28.824645322307855	22.071079057916073
30-31	22.205767659005314	27.083337276807264	28.716893389171776	21.994001675015646
32-33	22.04398638283676	27.260645890455887	28.671244397935208	22.024123328772145
34-35	22.174676646058405	27.339581013098286	28.680738016451734	21.80500432439158
36-37	22.203551729056542	27.220667341551042	28.504574857464142	22.071206071928273
38-39	22.11846125040533	27.299944303934996	28.597366677073843	21.984227768585832
40-41	22.31948240848169	27.20899901425985	28.47847389649798	21.99304468076048
42-43	22.24134886618669	27.2752586699838	28.31131216980018	22.172080294029335
44-45	22.27333698094274	27.439626976947544	28.10289552080975	22.184140521299963
46-47	22.456058034378675	27.373233449745697	27.746647590441746	22.42406092543388
48-49	22.37274870667607	27.498913592987346	27.731567244642157	22.39677045569443
50-51	22.31822963454577	27.65321339304902	27.653735502242938	22.37482147016227
52-53	22.50293994253125	27.577798012451062	27.593306793672586	22.325955251345107
54-55	22.50270071144759	27.533819153145423	27.52039973258181	22.44308040282517
56-57	22.483105742339635	27.664072423759904	27.459250289256826	22.39357154464364
58-59	22.370219843277646	27.707488267981002	27.581257470133735	22.34103441860762
60-61	22.52722853869055	27.740594318175148	27.461035115431088	22.271142027703213
62-63	22.44038671385772	27.8645650625988	27.42704394629455	22.268004277248927
64-65	22.427060046481998	27.753136457616257	27.411295161942604	22.408508333959134
66-67	22.493552401278503	27.736999819632587	27.289829991403202	22.479617787685715
68-69	22.40593393094243	27.70097125363468	27.378824785773077	22.514270029649815
70-71	22.435070469286874	27.547493236306963	27.413212622826233	22.60422367157993
72-73	22.497780464198215	27.5926376556072	27.274745766224562	22.634836113970024
74-75	22.368273080118435	27.70313422693988	27.305663795913187	22.622928897028498
76-77	22.51449403450517	27.583996386379738	27.2479721721878	22.653537406927292
78-79	22.47161158798269	27.614848887223925	27.142888379021162	22.770651145772224
80-81	22.502828377058222	27.671957696633413	27.185305617625545	22.63990830868282
82-83	22.513355783778678	27.623033057450787	27.260886392722313	22.602724766048226
84-85	22.54833364221059	27.660304247037455	27.210186252309292	22.58117585844266
86-87	22.488584404623587	27.643094417090147	27.29864157680576	22.5696796014805
88-89	22.468572746440387	27.628321694377018	27.25653567072255	22.64656988846005
90-91	22.510831965875852	27.66267626803711	27.267371966763456	22.55911979932358
92-93	22.55494103812862	27.599604888565477	27.29030839466433	22.555145678641573
94-95	22.372195931578556	27.64616739423442	27.35366865387785	22.627968020309176
96-97	22.508003411200594	27.738966784434304	27.393366342214698	22.359663462150404
98-99	23.645619710364173	29.636838031814623	27.534793922116336	19.18274833570487
100	22.545220245644995	30.64599219783434	21.427549869576254	25.381237686944413
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2286.0
1	1927.0
2	2255.5
3	3131.5
4	4172.0
5	6616.0
6	8665.0
7	10164.0
8	11247.0
9	11327.0
10	10593.5
11	9708.0
12	10665.5
13	14221.5
14	22514.0
15	34174.0
16	43856.5
17	49582.0
18	50887.5
19	48092.5
20	42963.5
21	37425.0
22	33764.0
23	34278.0
24	39162.0
25	47891.5
26	59650.0
27	73609.0
28	89296.0
29	107601.0
30	126456.0
31	145001.0
32	166061.0
33	187680.0
34	209446.0
35	230175.0
36	251093.0
37	270168.5
38	284954.5
39	298578.0
40	312117.0
41	324014.5
42	330546.0
43	336065.5
44	341722.5
45	347181.0
46	352880.0
47	357096.0
48	356934.5
49	352859.5
50	348697.0
51	339481.0
52	325464.0
53	308723.0
54	290034.0
55	271002.5
56	249531.5
57	225768.0
58	201253.0
59	174302.5
60	144967.5
61	117506.5
62	93672.0
63	72440.0
64	53721.5
65	39691.5
66	29926.0
67	22777.0
68	17371.0
69	12566.5
70	8961.0
71	6551.0
72	4531.5
73	2988.0
74	1950.0
75	1239.0
76	780.0
77	505.0
78	342.0
79	234.0
80	155.0
81	101.0
82	76.0
83	56.5
84	40.0
85	29.0
86	20.0
87	17.0
88	13.0
89	8.0
90	4.5
91	4.5
92	4.0
93	2.5
94	1.5
95	1.0
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	8.016638877560614E-5
7	0.0
8	0.0
9	0.0
10-11	0.00555438550802414
12-13	5.72617062682901E-6
14-15	0.00451794862456809
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	131.0
20-21	492.0
22-23	1019.0
24-25	2417.0
26-27	3127.0
28-29	5142.0
30-31	11540.0
32-33	14792.0
34-35	21165.0
36-37	30104.0
38-39	35633.0
40-41	40284.0
42-43	49877.0
44-45	61119.0
46-47	59554.0
48-49	52229.0
50-51	44103.0
52-53	42155.0
54-55	47362.0
56-57	50640.0
58-59	52775.0
60-61	53893.0
62-63	55291.0
64-65	57919.0
66-67	62162.0
68-69	59256.0
70-71	59141.0
72-73	56827.0
74-75	58733.0
76-77	57974.0
78-79	64004.0
80-81	65010.0
82-83	63920.0
84-85	64330.0
86-87	68270.0
88-89	74371.0
90-91	78944.0
92-93	83758.0
94-95	103124.0
96-97	298623.0
98-99	558466.0
100-101	6062163.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.1109862115218
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.6096220668432	76.2676330815077
2	7.280841029539882	12.393591209469022
3	1.78654753510134	4.561644678787152
4	0.6144346629886605	2.091805605180357
5	0.27868958914964964	1.1859772889705251
6	0.14723511251454613	0.7518795378646439
7	0.08508825030090954	0.5069361428686259
8	0.051025557222372495	0.347426839775085
9	0.030980256189042786	0.23730841416015303
>10	0.11250855631228367	1.42779328327317
>50	0.0025828811262420663	0.14640610262324666
>100	4.010882008115708E-4	0.05719207332405738
>500	4.341451115829565E-5	0.024405742196238853
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	5.726170626829011E-5	0.0	1.145234125365802E-5	0.0
3	0.0	5.726170626829011E-5	0.0	1.145234125365802E-5	0.0
4	0.0	9.161873002926416E-5	0.0	2.290468250731604E-5	0.0
5	0.0	1.030710712829222E-4	0.0	2.290468250731604E-5	0.0
6	0.0	1.030710712829222E-4	0.0	2.290468250731604E-5	1.145234125365802E-5
7	0.0	1.030710712829222E-4	0.0	2.290468250731604E-5	1.145234125365802E-5
8	0.0	1.3742809504389624E-4	0.0	2.290468250731604E-5	1.145234125365802E-5
9	0.0	1.8323746005852832E-4	0.0	3.435702376097406E-5	3.435702376097406E-5
10-11	0.0	1.9468980131218637E-4	0.0	4.008319438780307E-5	4.580936501463208E-5
12-13	0.0	2.004159719390154E-4	0.0	4.580936501463208E-5	5.726170626829011E-5
14-15	0.0	3.0348704322193755E-4	0.0	4.580936501463208E-5	9.161873002926416E-5
16-17	0.0	3.0921321384876657E-4	0.0	5.726170626829011E-5	1.030710712829222E-4
18-19	0.0	3.0921321384876657E-4	0.0	5.726170626829011E-5	1.087972419097512E-4
20-21	0.0	3.1493938447559554E-4	0.0	5.726170626829011E-5	1.2024958316340923E-4
22-23	5.72617062682901E-6	3.321178963560826E-4	0.0	8.016638877560614E-5	1.3170192441706722E-4
24-25	1.145234125365802E-5	3.779272613707147E-4	0.0	9.734490065609318E-5	1.3742809504389624E-4
26-27	1.145234125365802E-5	4.294627970121758E-4	0.0	1.3170192441706725E-4	1.4888043629755426E-4
28-29	1.145234125365802E-5	4.4664130889266287E-4	0.0	2.8630853134145053E-4	1.4888043629755426E-4
30-31	1.145234125365802E-5	4.6954599139997885E-4	0.0	8.131162290097195E-4	1.4888043629755426E-4
32-33	1.145234125365802E-5	4.809983326536369E-4	0.0	0.00219884952070234	1.717851188048703E-4
34-35	1.145234125365802E-5	5.66890892056072E-4	0.0	0.004036950291914452	1.717851188048703E-4
36-37	1.145234125365802E-5	6.642357927121652E-4	0.0	0.00723215350168504	1.775112894316993E-4
38-39	1.145234125365802E-5	6.985928164731393E-4	0.0	0.017510629776843114	1.8896363068535735E-4
40-41	1.145234125365802E-5	7.329498402341133E-4	0.0	0.028447615674086525	2.061421425658444E-4
42-43	1.145234125365802E-5	7.673068639950874E-4	0.0	0.03483229592300087	2.2904682507316043E-4
44-45	1.717851188048703E-5	8.245685702633776E-4	0.0	0.04310661247876879	2.2904682507316043E-4
46-47	3.435702376097406E-5	8.703779352780096E-4	0.0	0.05162715437149036	2.2904682507316043E-4
48-49	4.580936501463208E-5	8.761041059048387E-4	0.0	0.06063442076749239	2.4049916632681845E-4
50-51	4.580936501463208E-5	0.0010078060303219058	0.0	0.06943554502092858	2.4049916632681845E-4
52-53	4.580936501463208E-5	0.0010192583715755638	0.0	0.09406953105754698	2.5767767820730547E-4
54-55	4.580936501463208E-5	0.00104216305408288	0.0	0.1241949147252944	2.6913001946096346E-4
56-57	4.580936501463208E-5	0.001076520077843854	0.0	0.15752695394406607	2.748561900877925E-4
58-59	4.580936501463208E-5	0.001082246248470683	0.0	0.18970803286684512	2.748561900877925E-4
60-61	4.580936501463208E-5	0.001122329442858486	0.0	0.20227697739273479	2.748561900877925E-4
62-63	4.580936501463208E-5	0.001133781784112144	0.0	0.21820145790594628	2.8630853134145053E-4
64-65	4.580936501463208E-5	0.0012826622204096983	0.0	0.22396771172716307	2.8630853134145053E-4
66-67	5.72617062682901E-5	0.0012941145616633563	0.0	0.22826233969728482	2.8630853134145053E-4
68-69	6.871404752194812E-5	0.0013055669029170144	0.0	0.23249970596113834	2.8630853134145053E-4
70-71	6.871404752194812E-5	0.0013284715854243304	0.0	0.23631333559860646	2.977608725951085E-4
72-73	6.871404752194812E-5	0.0013399239266779885	0.0	0.2393310275189453	3.2066555510242456E-4
74-75	6.871404752194812E-5	0.0013800071210657914	0.0	0.24191925664227204	3.263917257292536E-4
76-77	6.871404752194812E-5	0.0014315426567072527	0.0	0.2442612604286451	3.321178963560826E-4
78-79	6.871404752194812E-5	0.0014544473392145687	0.0	0.24608790885860354	3.321178963560826E-4
80-81	8.016638877560614E-5	0.0014544473392145687	0.0	0.24662044272689865	3.4929640823656963E-4
82-83	8.016638877560614E-5	0.0014716258510950556	0.0	0.24703272701203033	3.6647492011705664E-4
84-85	8.016638877560614E-5	0.0014773520217218846	0.0	0.2472388691545962	3.6647492011705664E-4
86-87	8.016638877560614E-5	0.0015002567042292007	0.0	0.24731330937274495	3.6647492011705664E-4
88	8.016638877560614E-5	0.001557518410497491	0.0	0.24731330937274495	3.8937960262437274E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11670	0.0	52.629753	1
GTATCAA	21775	0.0	38.12833	1
TCAACGC	25960	0.0	31.260342	4
ATCAACG	26495	0.0	30.694704	3
CAACGCA	26730	0.0	30.36262	5
TATCAAC	27230	0.0	29.962166	2
AACGCAG	27300	0.0	29.664845	6
ACGCAGA	31295	0.0	25.919706	7
CGCAGAG	32295	0.0	25.090708	8
GTACATG	28035	0.0	22.653748	1
TACATGG	28540	0.0	21.810286	2
GCAGAGT	37225	0.0	21.710974	9
GTGGTAT	5565	0.0	21.055725	1
ACATGGG	28805	0.0	20.914013	3
GAGTACT	20995	0.0	20.635584	12-13
AGAGTAC	33605	0.0	19.259174	10-11
CAGAGTA	36575	0.0	19.208094	10-11
CATGGGG	21250	0.0	19.043306	4
TGGTATC	5715	0.0	18.978687	2
GTACTTT	23320	0.0	18.671944	14-15
>>END_MODULE