FastQCFastQC Report
Fri 27 May 2016
SRR2139523_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139523_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10043403
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA140700.052.630961
GTATCAA269700.039.256171
ATCAACG323200.032.1484343
TCAACGC333550.031.1511844
CAACGCA339900.030.545085
AACGCAG343750.030.2528136
TATCAAC348900.030.0627162
ACGCAGA350700.029.6654937
CGCAGAG409550.025.4136948
CTTATAC63850.023.07481
GCAGAGT468100.022.0618639
GTACATG360550.021.2750451
TACATGG359050.020.7794282
GAGTACT260650.020.51610812-13
ACATGGG349600.020.5080533
GTGGTAT70300.020.165691
TGGTATC66550.019.757442
AGAGTAC427700.019.24844410-11
CAGAGTA459300.019.17379810-11
TATACAC89250.019.147193
Produced by FastQC (version 0.11.5)