##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139523_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10043403
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.624531844435595	33.0	33.0	33.0	33.0	33.0
2	32.679139530694925	33.0	33.0	33.0	33.0	33.0
3	33.37461465999124	33.0	33.0	33.0	33.0	37.0
4	36.493651404807714	37.0	37.0	37.0	37.0	37.0
5	36.51667109245741	37.0	37.0	37.0	37.0	37.0
6	36.49674507734082	37.0	37.0	37.0	37.0	37.0
7	36.486130248880784	37.0	37.0	37.0	37.0	37.0
8	36.47062743574065	37.0	37.0	37.0	37.0	37.0
9	36.49656207164046	37.0	37.0	37.0	37.0	37.0
10-11	36.45088920558102	37.0	37.0	37.0	37.0	37.0
12-13	36.680087516153634	37.0	37.0	37.0	37.0	38.5
14-15	38.42316209953937	40.0	37.0	40.0	37.0	40.0
16-17	38.37481787796427	40.0	37.0	40.0	37.0	40.0
18-19	38.38769359349615	40.0	37.0	40.0	37.0	40.0
20-21	38.387624568279605	40.0	37.0	40.0	37.0	40.0
22-23	38.39123017966537	40.0	37.0	40.0	37.0	40.0
24-25	38.359689690810356	40.0	37.0	40.0	37.0	40.0
26-27	38.25486209495253	40.0	37.0	40.0	37.0	40.0
28-29	38.180256678395594	40.0	37.0	40.0	37.0	40.0
30-31	38.059916845454936	40.0	37.0	40.0	33.0	40.0
32-33	38.05406582283935	40.0	37.0	40.0	33.0	40.0
34-35	38.01188985378583	40.0	37.0	40.0	33.0	40.0
36-37	37.93715003602158	40.0	37.0	40.0	33.0	40.0
38-39	37.88619904948327	40.0	37.0	40.0	33.0	40.0
40-41	37.89065052112463	40.0	37.0	40.0	33.0	40.0
42-43	37.82309838754024	40.0	37.0	40.0	33.0	40.0
44-45	37.7638497233925	40.0	37.0	40.0	33.0	40.0
46-47	37.73098172053219	40.0	37.0	40.0	33.0	40.0
48-49	37.69583709964129	40.0	37.0	40.0	33.0	40.0
50-51	37.60655450104647	40.0	37.0	40.0	33.0	40.0
52-53	37.49992545331474	40.0	37.0	40.0	33.0	40.0
54-55	37.35023099762995	40.0	37.0	40.0	33.0	40.0
56-57	37.23303450776487	38.5	37.0	40.0	33.0	40.0
58-59	37.08068929494957	37.0	37.0	40.0	33.0	40.0
60-61	36.953421661626166	37.0	37.0	40.0	33.0	40.0
62-63	36.782245611937626	37.0	37.0	40.0	33.0	40.0
64-65	36.6018240965793	37.0	37.0	40.0	33.0	40.0
66-67	36.36849958034262	37.0	37.0	40.0	33.0	40.0
68-69	36.14012413839649	37.0	37.0	40.0	33.0	40.0
70-71	35.89517741186296	37.0	35.0	37.0	33.0	40.0
72-73	35.68142636521832	37.0	33.0	37.0	33.0	40.0
74-75	35.25408946447244	37.0	33.0	37.0	33.0	40.0
76-77	33.967928685393744	35.0	33.0	37.0	27.0	37.0
78-79	35.01331824150547	37.0	33.0	37.0	33.0	37.0
80-81	35.05633025016067	37.0	33.0	37.0	33.0	37.0
82-83	34.909943164805014	37.0	33.0	37.0	33.0	37.0
84-85	34.78112703550826	37.0	33.0	37.0	33.0	37.0
86-87	34.66816623167002	37.0	33.0	37.0	33.0	37.0
88-89	34.52751021590716	37.0	33.0	37.0	33.0	37.0
90-91	34.52512580762797	37.0	33.0	37.0	33.0	37.0
92-93	34.45671328077085	37.0	33.0	37.0	33.0	37.0
94-95	34.43719776930905	37.0	33.0	37.0	33.0	37.0
96-97	34.44009026313283	37.0	33.0	37.0	33.0	37.0
98-99	34.42873487186711	37.0	33.0	37.0	33.0	37.0
100	34.42076609524885	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	1.0
17	0.0
18	3.0
19	18.0
20	98.0
21	438.0
22	1568.0
23	4478.0
24	10129.0
25	19841.0
26	34800.0
27	54747.0
28	81721.0
29	113761.0
30	154392.0
31	206240.0
32	273070.0
33	366141.0
34	503527.0
35	725111.0
36	1249530.0
37	2881519.0
38	3261625.0
39	100644.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.537642968225015	0.0	24.776432848507625	29.685924183267364
2	15.012122883050697	24.16353301764352	38.146044722092704	22.678299377213083
3	16.336425014509523	25.132188761120112	33.134755221910346	25.396631002460023
4	13.035472140269588	23.384643631247297	37.35674053903841	26.223143689444704
5	17.286493532665645	36.63471996922157	32.24559700722743	13.833189490885353
6	27.873811108363032	37.3837294745558	20.41628781044803	14.326171606633139
7	26.267715488010484	32.832563391624014	22.551534086909406	18.3481870334561
8	24.521822646262017	31.942339827849082	22.055894036649153	21.479943489239748
9	24.20741580947691	20.250073157634866	23.34210959436867	32.20040143851956
10-11	24.81884672172645	25.913226749533646	28.555283842855633	20.71264268588427
12-13	25.305150176367512	24.029911460535498	28.30881692204149	22.356121441055492
14-15	22.68111721481606	26.329387086539345	26.3605326265712	24.628963072073397
16-17	21.687186106143503	28.642687144984624	27.326863215585394	22.343263533286475
18-19	21.853359862190135	27.63075423738349	29.071615467386902	21.444270433039478
20-21	22.719908059692695	26.924756234119595	28.914024819979772	21.441310886207937
22-23	22.593824874706154	26.716893411628316	28.71603344010616	21.97324827355937
24-25	22.184499461175744	27.06825570394492	28.941672865673024	21.805571969206312
26-27	22.12559262991899	27.32228749830424	28.72554307875163	21.826576793025133
28-29	22.02464326424555	27.061841641166907	28.900097240363543	22.013417854223995
30-31	22.361156262498064	27.05593468859847	28.77570811859223	21.807200930311236
32-33	22.086462171949965	27.329838985180032	28.664789021906778	21.91890982096323
34-35	22.265497458604997	27.141345016194013	28.693736597270618	21.89942092793037
36-37	22.275268344241475	27.182406708086887	28.61197204212938	21.930352905542254
38-39	22.06817116043491	27.415501357291568	28.627917828305204	21.888409653968317
40-41	22.39003770553723	27.07086519075652	28.420323315547876	22.118773788158375
42-43	22.29917518161461	27.131836814466322	28.381667239368504	22.187320764550567
44-45	22.297172094936155	27.54515342968049	28.007691653815236	22.14998282156812
46-47	22.516755407843167	27.215665495460073	27.699291360367774	22.568287736328987
48-49	22.344464382150154	27.46294478162016	27.747690268838554	22.444900567391134
50-51	22.37614139283998	27.71449598969798	27.47233041394992	22.437032203512118
52-53	22.599781727760828	27.44130821502114	27.45834298362618	22.500567073591846
54-55	22.534362877004373	27.444109474917234	27.529316958747447	22.49221068933095
56-57	22.55554593758425	27.802527904318698	27.24167995238674	22.40024620571032
58-59	22.508915566287705	27.59237641984404	27.451156214999067	22.447551798869185
60-61	22.5925123201406	27.741311930672595	27.32849727855732	22.33767847062948
62-63	22.498034445952374	27.96719857174056	27.20804585710473	22.326721125202333
64-65	22.58406604045114	27.596796931922363	27.231943945642133	22.587193081984363
66-67	22.584344098417567	27.629772779276962	27.162011219509242	22.62387190279623
68-69	22.557912441639584	27.738941020755803	27.19926809063399	22.503878446970628
70-71	22.634062542075384	27.308291510058087	27.32642616052211	22.73121978734442
72-73	22.576604506689876	27.418399462810232	27.234866528902398	22.770129501597495
74-75	22.488880584586767	27.702962334122695	27.17377970762538	22.634377373665163
76-77	22.730014120490168	27.371358353686663	27.10442723029981	22.79420029552336
78-79	22.55284784744807	27.42396342549902	27.21733857455675	22.80585015249616
80-81	22.576195877403133	27.659783023980665	27.087865002456795	22.676156096159403
82-83	22.78947521589921	27.31018586832879	27.19632622027362	22.704012695498378
84-85	22.759707331428768	27.352093552487084	27.21561030491117	22.672588811172982
86-87	22.598259162536273	27.52502386981125	27.200104192717667	22.676612774934814
88-89	22.75030450579018	27.257375768913654	27.216095069736273	22.77622465555989
90-91	22.733118541902964	27.307475930399118	27.253924971745036	22.70548055595288
92-93	22.732662470788547	27.385784318665618	27.25700452980834	22.62454868073749
94-95	22.713348618031056	27.144262662415013	27.350200264577197	22.79218845497673
96-97	22.750076421219173	27.490492935098658	27.41444304679631	22.34498759688586
98-99	23.88977394972283	29.605799546482984	26.866287947264738	19.638138556529448
100	23.02783661404351	29.774614740131984	21.272218927391343	25.925329718433165
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1641.0
1	1545.0
2	2498.0
3	3785.0
4	5269.0
5	8847.0
6	11745.5
7	13471.0
8	14479.5
9	14178.5
10	13039.5
11	11672.5
12	12689.0
13	16739.5
14	26875.0
15	42993.5
16	57966.5
17	67598.5
18	69791.0
19	64533.0
20	55576.0
21	46400.0
22	39959.5
23	38542.0
24	42023.5
25	50888.0
26	64319.0
27	80497.5
28	98645.0
29	120934.0
30	144016.0
31	166348.0
32	192522.5
33	220524.0
34	247765.5
35	273551.0
36	301421.5
37	326116.0
38	342990.5
39	357054.5
40	369366.5
41	378089.0
42	382477.5
43	388738.0
44	396268.5
45	402573.0
46	407517.5
47	411485.0
48	414191.0
49	411461.0
50	406538.5
51	397067.0
52	381697.0
53	363088.0
54	341642.5
55	317454.5
56	291230.0
57	264164.5
58	237276.0
59	208221.0
60	175212.5
61	142378.5
62	113231.0
63	88155.5
64	65998.5
65	49241.0
66	37182.0
67	28205.0
68	21585.0
69	15701.5
70	11135.0
71	8138.0
72	5741.5
73	4014.0
74	2755.5
75	1756.0
76	1129.0
77	730.5
78	488.0
79	334.5
80	225.5
81	148.0
82	104.5
83	78.0
84	63.0
85	52.0
86	42.5
87	34.5
88	21.0
89	10.0
90	7.0
91	5.5
92	5.0
93	3.5
94	2.0
95	1.5
96	2.0
97	2.0
98	1.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	6.96974919755784E-5
6	1.4935176851909655E-4
7	1.9913569135879542E-5
8	5.974070740763863E-5
9	2.1904926049467498E-4
10-11	0.0031811926694567567
12-13	2.8376836018628347E-4
14-15	0.0023049956274780567
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	228.0
20-21	690.0
22-23	1421.0
24-25	3841.0
26-27	4716.0
28-29	8553.0
30-31	16726.0
32-33	21958.0
34-35	31048.0
36-37	43420.0
38-39	52312.0
40-41	56649.0
42-43	70162.0
44-45	84196.0
46-47	80671.0
48-49	70929.0
50-51	62080.0
52-53	61126.0
54-55	66472.0
56-57	71120.0
58-59	71781.0
60-61	72216.0
62-63	70784.0
64-65	75402.0
66-67	80395.0
68-69	77425.0
70-71	76681.0
72-73	74784.0
74-75	76370.0
76-77	75913.0
78-79	81446.0
80-81	83098.0
82-83	81629.0
84-85	84298.0
86-87	89370.0
88-89	95931.0
90-91	102146.0
92-93	109054.0
94-95	145019.0
96-97	505942.0
98-99	1794051.0
100-101	5311350.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	80.70122164928495
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.3175415249985	70.46632272479548
2	8.339705498615109	13.460488438669968
3	2.3024529801199565	5.574323048571518
4	0.9017887075894278	2.911018014879865
5	0.4279362625767097	1.7267489588984826
6	0.22109632614768895	1.0705646173372347
7	0.14204991699645028	0.8024521285755145
8	0.08962935056693536	0.5786558469106972
9	0.055447234657528745	0.40271936165434274
>10	0.1959969496093067	2.483420895051043
>50	0.004898981711561608	0.2670054777277326
>100	0.0013106341680820355	0.16120131702908844
>500	1.21360342137468E-4	0.06873368599577098
>1k	2.4271900689904504E-5	0.026345483903371842
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	5.9740707407638625E-5	0.0	0.0	0.0
3	0.0	5.9740707407638625E-5	0.0	0.0	0.0
4	0.0	5.9740707407638625E-5	0.0	0.0	0.0
5	0.0	6.969749197557839E-5	0.0	0.0	0.0
6	9.956784567939772E-5	6.969749197557839E-5	0.0	0.0	0.0
7	9.956784567939772E-5	6.969749197557839E-5	0.0	0.0	0.0
8	9.956784567939772E-5	1.0952463024733748E-4	0.0	0.0	0.0
9	9.956784567939772E-5	2.1904926049467495E-4	0.0	0.0	9.956784567939771E-6
10-11	1.045462379633676E-4	2.1904926049467495E-4	0.0	0.0	9.956784567939771E-6
12-13	1.0952463024733748E-4	2.2402765277864485E-4	0.0	0.0	9.956784567939771E-6
14-15	1.0952463024733748E-4	3.3853067530995224E-4	0.0	0.0	4.480553055572897E-5
16-17	1.5930855308703633E-4	3.48487459877892E-4	0.0	0.0	5.9740707407638625E-5
18-19	1.6428694537100623E-4	3.48487459877892E-4	0.0	9.956784567939771E-6	5.9740707407638625E-5
20-21	1.74243729938946E-4	3.5844424444583177E-4	0.0	2.489196141984943E-5	5.9740707407638625E-5
22-23	1.8420051450688575E-4	4.082281672855306E-4	0.0	2.9870353703819313E-5	6.969749197557839E-5
24-25	1.8917890679085564E-4	4.281417364214101E-4	0.0	4.480553055572897E-5	7.965427654351817E-5
26-27	1.8917890679085564E-4	4.430769132733198E-4	0.0	8.463266882748806E-5	7.965427654351817E-5
28-29	2.090924759267352E-4	4.480553055572897E-4	0.0	2.2402765277864485E-4	7.965427654351817E-5
30-31	2.090924759267352E-4	5.575799358046271E-4	0.0	6.621261737679948E-4	7.965427654351817E-5
32-33	2.1407086821070506E-4	5.62558328088597E-4	0.0	0.0015881071385863935	8.961106111145794E-5
34-35	2.2900604506261474E-4	7.019533120397538E-4	0.0	0.0027978564635910757	8.961106111145794E-5
36-37	2.2900604506261474E-4	8.21434726855031E-4	0.0	0.0048539324768706385	8.961106111145794E-5
38-39	2.2900604506261474E-4	8.712186496947299E-4	0.0	0.007860881416388448	8.961106111145794E-5
40-41	2.4891961419849426E-4	9.458945339542782E-4	0.0	0.01083796000220244	8.961106111145794E-5
42-43	2.4891961419849426E-4	0.0010255488104977963	0.0	0.014168504440178295	8.961106111145794E-5
44-45	2.8874675247025336E-4	0.0011002246947573447	0.0	0.01785251473031601	9.458945339542782E-5
46-47	2.9372514475422325E-4	0.0011052030870413146	0.0	0.021860120518911768	9.956784567939772E-5
48-49	3.0368192932216304E-4	0.0011052030870413146	0.0	0.025872704699791495	1.2943819938321703E-4
50-51	3.3853067530995224E-4	0.0012744684246962907	0.0	0.029850440134683433	1.2943819938321703E-4
52-53	3.6342263672980166E-4	0.0012844252092642304	0.0	0.03368878058562422	1.4935176851909655E-4
54-55	3.6840102901377155E-4	0.0013192739552520196	0.0	0.037810889396751285	1.692653376549761E-4
56-57	3.6840102901377155E-4	0.0013541227012398088	0.0	0.04256525402794252	1.692653376549761E-4
58-59	3.6840102901377155E-4	0.0013640794858077487	0.0	0.047324597051417734	1.692653376549761E-4
60-61	3.783578135817113E-4	0.0013690578780917186	0.0	0.05195948026779369	1.692653376549761E-4
62-63	3.9827138271759086E-4	0.001388971447227598	0.0	0.0564051845773788	1.692653376549761E-4
64-65	4.082281672855306E-4	0.0016229558845741827	0.0	0.061015175832334914	1.692653376549761E-4
66-67	4.530336978412596E-4	0.0016229558845741827	0.0	0.06571477814840249	1.692653376549761E-4
68-69	4.580120901252295E-4	0.0016727398074138815	0.0	0.07081763023947162	1.692653376549761E-4
70-71	4.77925659261109E-4	0.0017324805148215203	0.0	0.07518368027251321	1.8917890679085564E-4
72-73	5.277095821008079E-4	0.0017723076530932794	0.0	0.07824041313487072	1.9913569135879543E-4
74-75	5.277095821008079E-4	0.0018121347913650384	0.0	0.08091380979136255	1.9913569135879543E-4
76-77	5.476231512366874E-4	0.001916681029328406	0.0	0.08347270342532306	1.9913569135879543E-4
78-79	5.476231512366874E-4	0.001961486559884135	0.0	0.08499609146421785	1.9913569135879543E-4
80-81	5.526015435206573E-4	0.0019814001290200146	0.0	0.08556362818459043	2.0411408364276533E-4
82-83	5.575799358046271E-4	0.001991356913587954	0.0	0.08607142419755534	2.090924759267352E-4
84-85	5.774935049405067E-4	0.0019963353058719243	0.0	0.08633527898860575	2.090924759267352E-4
86-87	5.824718972244766E-4	0.002011270482723834	0.0	0.08638008451916149	2.090924759267352E-4
88	5.874502895084465E-4	0.0021904926049467494	0.0	0.08638506291144546	2.090924759267352E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	14070	0.0	52.63096	1
GTATCAA	26970	0.0	39.25617	1
ATCAACG	32320	0.0	32.148434	3
TCAACGC	33355	0.0	31.151184	4
CAACGCA	33990	0.0	30.54508	5
AACGCAG	34375	0.0	30.252813	6
TATCAAC	34890	0.0	30.062716	2
ACGCAGA	35070	0.0	29.665493	7
CGCAGAG	40955	0.0	25.413694	8
CTTATAC	6385	0.0	23.0748	1
GCAGAGT	46810	0.0	22.061863	9
GTACATG	36055	0.0	21.275045	1
TACATGG	35905	0.0	20.779428	2
GAGTACT	26065	0.0	20.516108	12-13
ACATGGG	34960	0.0	20.508053	3
GTGGTAT	7030	0.0	20.16569	1
TGGTATC	6655	0.0	19.75744	2
AGAGTAC	42770	0.0	19.248444	10-11
CAGAGTA	45930	0.0	19.173798	10-11
TATACAC	8925	0.0	19.14719	3
>>END_MODULE