FastQCFastQC Report
Fri 27 May 2016
SRR2139522_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139522_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9484645
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA203950.048.1332471
CTTATAC83050.041.48821
GTATCAA356650.036.4510081
TATACAC106500.033.9410633
ATCAACG431900.029.738123
TATCAAC453850.028.448912
TCAACGC455000.028.2004624
ACGCAGA457550.028.0639977
TTATACA124200.027.9126572
CAACGCA460000.027.8868035
AACGCAG465850.027.6001426
ACACATC140050.024.3328466
CGCAGAG535900.023.953588
ATACACA164100.022.9808034
TACACAT155350.022.888945
ACATCTC159700.021.57778
GCAGAGT600900.021.2294779
GTGGTAT95450.020.9060841
ACATGGG416800.020.7431413
GAGTACT333800.020.68397312-13
Produced by FastQC (version 0.11.5)