##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139522_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9484645
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.60854581273205	33.0	33.0	33.0	33.0	33.0
2	32.664900162314986	33.0	33.0	33.0	33.0	33.0
3	33.34770020385581	33.0	33.0	33.0	33.0	37.0
4	36.4730690500277	37.0	37.0	37.0	37.0	37.0
5	36.500198056964706	37.0	37.0	37.0	37.0	37.0
6	36.47852681887409	37.0	37.0	37.0	37.0	37.0
7	36.470841344088264	37.0	37.0	37.0	37.0	37.0
8	36.45096954076826	37.0	37.0	37.0	37.0	37.0
9	36.48641725652357	37.0	37.0	37.0	37.0	37.0
10-11	36.434979643413115	37.0	37.0	37.0	37.0	37.0
12-13	36.655664550439155	37.0	37.0	37.0	37.0	38.5
14-15	38.36342340699098	40.0	37.0	40.0	37.0	40.0
16-17	38.305274050847444	40.0	37.0	40.0	37.0	40.0
18-19	38.3340278945601	40.0	37.0	40.0	37.0	40.0
20-21	38.33652938088848	40.0	37.0	40.0	37.0	40.0
22-23	38.34124344450204	40.0	37.0	40.0	37.0	40.0
24-25	38.30307730316915	40.0	37.0	40.0	37.0	40.0
26-27	38.18382376260726	40.0	37.0	40.0	37.0	40.0
28-29	38.11142232540536	40.0	37.0	40.0	35.0	40.0
30-31	37.96771880709882	40.0	37.0	40.0	33.0	40.0
32-33	37.95166271345978	40.0	37.0	40.0	33.0	40.0
34-35	37.90921819445188	40.0	37.0	40.0	33.0	40.0
36-37	37.82591408016676	40.0	37.0	40.0	33.0	40.0
38-39	37.76064467512617	40.0	37.0	40.0	33.0	40.0
40-41	37.76662179147269	40.0	37.0	40.0	33.0	40.0
42-43	37.69855965765926	40.0	37.0	40.0	33.0	40.0
44-45	37.62777339123104	40.0	37.0	40.0	33.0	40.0
46-47	37.59174648142499	40.0	37.0	40.0	33.0	40.0
48-49	37.55393464064626	40.0	37.0	40.0	33.0	40.0
50-51	37.44354541577977	40.0	37.0	40.0	33.0	40.0
52-53	37.34617554849391	40.0	37.0	40.0	33.0	40.0
54-55	37.20450148733254	38.5	37.0	40.0	33.0	40.0
56-57	37.08179634994707	37.0	37.0	40.0	33.0	40.0
58-59	36.92982322704911	37.0	37.0	40.0	33.0	40.0
60-61	36.803167428612326	37.0	37.0	40.0	33.0	40.0
62-63	36.639778876618294	37.0	37.0	40.0	33.0	40.0
64-65	36.46559378268802	37.0	37.0	40.0	33.0	40.0
66-67	36.241980324538815	37.0	37.0	40.0	33.0	40.0
68-69	36.012747407512904	37.0	37.0	38.5	33.0	40.0
70-71	35.77273112630574	37.0	33.0	37.0	33.0	40.0
72-73	35.56401356772682	37.0	33.0	37.0	33.0	40.0
74-75	35.14944277135792	37.0	33.0	37.0	30.0	40.0
76-77	33.85760943873301	35.0	33.0	37.0	27.0	37.0
78-79	34.92329958583997	37.0	33.0	37.0	33.0	37.0
80-81	34.97870224599016	37.0	33.0	37.0	33.0	37.0
82-83	34.83535948524267	37.0	33.0	37.0	33.0	37.0
84-85	34.71555086370118	37.0	33.0	37.0	33.0	37.0
86-87	34.60245189836623	37.0	33.0	37.0	33.0	37.0
88-89	34.46376669180573	37.0	33.0	37.0	33.0	37.0
90-91	34.46522150005319	37.0	33.0	37.0	33.0	37.0
92-93	34.394495927840424	37.0	33.0	37.0	33.0	37.0
94-95	34.37867480463075	37.0	33.0	37.0	33.0	37.0
96-97	34.38748164978411	37.0	33.0	37.0	33.0	37.0
98-99	34.387040154222426	37.0	33.0	37.0	33.0	37.0
100	34.37336729102577	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	0.0
17	0.0
18	7.0
19	23.0
20	99.0
21	394.0
22	1693.0
23	4639.0
24	10298.0
25	20216.0
26	34636.0
27	55230.0
28	82046.0
29	115389.0
30	155073.0
31	206372.0
32	274181.0
33	364235.0
34	495405.0
35	709814.0
36	1224171.0
37	2778545.0
38	2867555.0
39	84623.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.961920556857955	0.0	24.446766325993224	29.59131311714882
2	15.290029305261294	23.985251951970792	38.44783858541885	22.276880157349062
3	16.445939726789984	25.18275591759101	33.12798739436215	25.243316961256852
4	12.913451162378772	23.802746439112905	37.06900996294537	26.214792435562956
5	17.14788843396636	36.33451464646587	32.2717259890541	14.245870930513673
6	27.51563792880947	37.529236769544674	20.183292298530926	14.771833003114935
7	26.016980711136867	32.76853617278624	22.437225899042705	18.777257217034187
8	24.469199338635523	31.644647542540916	21.98220827498457	21.903944843838993
9	24.522901487713167	20.13885843023403	22.718538974238726	32.61970110781408
10-11	24.859873981476795	25.919517688142115	28.024492003643907	21.196116326737183
12-13	25.634663928762187	24.05587737460602	27.882232783875715	22.427225912756075
14-15	22.97843170560258	25.97124698360924	26.3729632278415	24.677358082946675
16-17	21.673320403662974	28.539639596421374	27.26465249885473	22.522387501060926
18-19	21.782992405092653	27.15698373529004	29.389297121821638	21.67072673779567
20-21	22.94856753037212	26.629656116085304	29.07381277293656	21.34796358060602
22-23	22.708663649241664	26.331034156222525	28.922737304282638	22.037564890253176
24-25	22.275480940961966	26.705858384640855	29.237243463917252	21.781417210479926
26-27	22.064081348646493	27.08681513979319	29.158139560405466	21.690963951154853
28-29	21.807859068188705	26.711356825382783	29.31308455599285	22.167699550435664
30-31	22.15055663311235	26.827209499403722	29.138585998416154	21.883647869067783
32-33	21.824095898870713	27.021848218755153	29.312406936531826	21.84164894584231
34-35	21.8580205685675	26.82406296773102	29.08086223276812	22.237054230933364
36-37	21.96639133481076	27.090253316335648	29.166074789445574	21.77728055940802
38-39	21.89203687126883	27.17561174728188	29.083660849740628	21.848690531708662
40-41	22.15175385698452	26.93714101849117	28.940316548875472	21.970788575648832
42-43	22.44168918948676	26.813340976475374	28.707149982489753	22.037819851548115
44-45	22.229522768330508	27.630307660863874	28.165548951533363	21.974620619272255
46-47	22.463514232468814	27.07420904942638	27.687753543213905	22.774523174890902
48-49	22.366951092751727	27.12480622586224	27.956382897696212	22.551859783689817
50-51	22.182900232283277	27.60260797083056	27.57290467506944	22.641587121816723
52-53	22.401672298826515	27.512341693480806	27.613022394940906	22.472963612751773
54-55	22.46280133837316	27.48087501816198	27.476276467611356	22.580047175853505
56-57	22.74937618024742	27.965846306063145	26.868654809670396	22.416122704019042
58-59	22.44415313692459	27.833333819927187	27.517308629936615	22.205204413211607
60-61	22.590897106461174	27.9623304147022	27.064502313963207	22.382270164873415
62-63	22.44802126571575	28.17215140633496	26.960379606167173	22.419447721782124
64-65	22.412179724499826	27.949895648969235	27.0142319574341	22.62369266909684
66-67	22.59756805034193	27.798055724456965	26.873382974264686	22.730993250936418
68-69	22.693381295369846	27.752197697964494	27.091242035502354	22.463178971163302
70-71	22.756352283434797	27.19334050645934	27.17595861326662	22.874348596839244
72-73	22.63484831019649	27.40629600855437	27.149839855477886	22.809015825771255
74-75	22.49013766880587	27.690318922971457	27.13805737878407	22.68148602943861
76-77	22.728733703126732	27.418861881471607	26.9496129819717	22.902791433429964
78-79	22.669748080717323	27.575148014070272	27.019203459221984	22.73590044599042
80-81	22.610481748023325	27.596691204103298	26.973828796826155	22.818998251047223
82-83	22.954927677842825	27.24118408717093	27.12433424250068	22.679553992485562
84-85	22.9860897260416	27.188177191566776	27.026749599098288	22.798983483293338
86-87	22.63834036708027	27.525158914093613	27.041701292815233	22.794799426010883
88-89	22.86973848704362	27.101169429906307	27.088442914190487	22.940649168859583
90-91	22.887117059151546	27.1859992278986	27.14252820314846	22.784355509801397
92-93	22.821687197720074	27.293428469926802	27.155618004219807	22.72926632813332
94-95	22.79839228398038	27.192809006544106	27.12079451887378	22.888004190601734
96-97	22.75809314385417	27.423417518403774	27.375684710903702	22.44280462683835
98-99	23.896299266908468	29.555687373490453	26.812186371391633	19.73582698820945
100	23.218442228483827	29.936072796974184	20.99166175839827	25.853823216143724
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2256.0
1	2140.5
2	3573.5
3	5349.0
4	7570.5
5	12974.5
6	16955.5
7	18771.5
8	19278.0
9	17957.5
10	15980.0
11	14241.0
12	15856.0
13	21566.5
14	35818.0
15	57908.0
16	77677.5
17	89739.0
18	91704.5
19	84405.5
20	72007.5
21	58537.5
22	48444.0
23	44656.5
24	47152.5
25	55823.5
26	70676.5
27	88824.5
28	104066.5
29	120570.0
30	136009.0
31	150287.0
32	171112.5
33	193500.5
34	217464.0
35	241420.0
36	270414.5
37	296781.0
38	302554.5
39	305866.0
40	320337.5
41	330959.5
42	334216.0
43	341011.0
44	352591.0
45	363345.5
46	367423.5
47	374216.5
48	395165.0
49	408240.5
50	408118.5
51	406403.0
52	396870.5
53	376767.5
54	350133.0
55	320901.0
56	292465.0
57	268717.0
58	245091.5
59	215083.5
60	178003.5
61	140633.5
62	106518.5
63	78193.5
64	57702.0
65	41593.0
66	30393.5
67	22626.0
68	16655.5
69	12237.5
70	8698.5
71	6150.0
72	4375.5
73	3074.0
74	2129.0
75	1482.0
76	989.0
77	613.0
78	405.0
79	282.5
80	208.5
81	143.0
82	101.5
83	74.5
84	55.5
85	38.0
86	26.5
87	19.0
88	13.0
89	9.0
90	6.5
91	5.0
92	3.0
93	1.5
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	2.108671436832902E-5
6	1.3706364339413862E-4
7	1.054335718416451E-5
8	1.1597692902580961E-4
9	2.214105008674547E-4
10-11	0.0034265910848534655
12-13	2.003237864991257E-4
14-15	0.002256278437411205
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	233.0
20-21	961.0
22-23	1909.0
24-25	3662.0
26-27	5561.0
28-29	10703.0
30-31	18708.0
32-33	25332.0
34-35	36586.0
36-37	47602.0
38-39	57129.0
40-41	61866.0
42-43	78530.0
44-45	95915.0
46-47	91317.0
48-49	79074.0
50-51	66619.0
52-53	65188.0
54-55	74578.0
56-57	79929.0
58-59	80993.0
60-61	80773.0
62-63	77597.0
64-65	80870.0
66-67	89019.0
68-69	77357.0
70-71	75698.0
72-73	72493.0
74-75	73114.0
76-77	73167.0
78-79	78312.0
80-81	80825.0
82-83	78150.0
84-85	80109.0
86-87	84095.0
88-89	91231.0
90-91	96762.0
92-93	104186.0
94-95	138616.0
96-97	460819.0
98-99	1636327.0
100-101	4872730.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	77.33139960600276
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.06008911926945	66.55147141810436
2	8.597059232213043	13.296452458454844
3	2.6636662156748687	6.179551096240872
4	1.1042828635388633	3.415829575935394
5	0.56912754459028	2.200571478874705
6	0.31451780677646013	1.4593261319420403
7	0.20173510807987957	1.0920320780239727
8	0.1208669164049008	0.7477446249322172
9	0.0852780329855414	0.5935202681776908
>10	0.276156114163699	3.2149762837834626
>50	0.004228137803113562	0.22192813967627328
>100	0.0022532223054531784	0.36388910279202874
>500	3.550495911163274E-4	0.1818305118708939
>1k	3.8463660326418616E-4	0.48087683119142366
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.054335718416451E-5	0.0	0.0	0.0
3	0.0	1.054335718416451E-5	0.0	0.0	0.0
4	0.0	1.054335718416451E-5	0.0	1.054335718416451E-5	0.0
5	0.0	1.054335718416451E-5	0.0	1.054335718416451E-5	0.0
6	1.054335718416451E-5	1.054335718416451E-5	0.0	2.108671436832902E-5	0.0
7	1.054335718416451E-5	1.054335718416451E-5	0.0	2.108671436832902E-5	0.0
8	1.054335718416451E-5	3.163007155249353E-5	0.0	3.163007155249353E-5	0.0
9	1.054335718416451E-5	4.217342873665804E-5	0.0	3.163007155249353E-5	0.0
10-11	1.054335718416451E-5	4.217342873665804E-5	0.0	6.853182169706931E-5	0.0
12-13	1.054335718416451E-5	4.217342873665804E-5	0.0	7.380350028915158E-5	0.0
14-15	1.5815035776246762E-5	6.853182169706931E-5	0.0	8.434685747331607E-5	0.0
16-17	2.6358392960411275E-5	8.434685747331607E-5	0.0	1.054335718416451E-4	0.0
18-19	3.163007155249353E-5	8.434685747331607E-5	0.0	1.423353219862209E-4	0.0
20-21	3.163007155249353E-5	8.434685747331607E-5	0.0	1.6869371494663215E-4	0.0
22-23	3.163007155249353E-5	8.434685747331607E-5	0.0	2.1613882227537246E-4	0.0
24-25	4.217342873665804E-5	1.0016189324956284E-4	0.0	2.47768893827866E-4	0.0
26-27	4.217342873665804E-5	1.1597692902580961E-4	0.0	3.479307870774288E-4	0.0
28-29	4.217342873665804E-5	1.2124860761789186E-4	0.0	6.484164668261174E-4	0.0
30-31	4.217342873665804E-5	1.3706364339413862E-4	0.0	0.0013126479694284816	0.0
32-33	4.217342873665804E-5	1.3706364339413862E-4	0.0	0.0024091571165815906	0.0
34-35	4.217342873665804E-5	1.5815035776246765E-4	0.0	0.0037745218719308945	0.0
36-37	4.217342873665804E-5	1.7923707213079668E-4	0.0	0.005935910094684619	0.0
38-39	4.217342873665804E-5	1.7923707213079668E-4	0.0	0.009320327750801428	0.0
40-41	5.271678592082255E-5	1.7923707213079668E-4	0.0	0.012941970943561937	0.0
42-43	5.271678592082255E-5	1.9505210790704344E-4	0.0	0.016147151527547948	0.0
44-45	5.271678592082255E-5	2.108671436832902E-4	0.0	0.01982678318482136	0.0
46-47	5.271678592082255E-5	2.108671436832902E-4	0.0	0.02363293512830475	0.0
48-49	5.271678592082255E-5	2.108671436832902E-4	0.0	0.02760778078673477	0.0
50-51	5.271678592082255E-5	2.4249721523578372E-4	0.0	0.031403389373033996	0.0
52-53	5.271678592082255E-5	2.4249721523578372E-4	0.0	0.03527807313821445	0.0
54-55	5.271678592082255E-5	2.4249721523578372E-4	0.0	0.03882064115209373	0.0
56-57	5.271678592082255E-5	2.5304057241994825E-4	0.0	0.04280603016770791	0.0
58-59	5.271678592082255E-5	2.5304057241994825E-4	0.0	0.04659636707541505	0.0
60-61	5.271678592082255E-5	2.5304057241994825E-4	0.0	0.05046577916200343	0.0
62-63	5.271678592082255E-5	2.5304057241994825E-4	0.0	0.05404524892602728	0.0
64-65	5.271678592082255E-5	2.635839296041128E-4	0.0	0.057888302619655246	0.0
66-67	5.271678592082255E-5	2.7412728678827725E-4	0.0	0.061910593385414006	0.0
68-69	5.271678592082255E-5	2.793989653803595E-4	0.0	0.06585908065088361	0.0
70-71	5.271678592082255E-5	2.846706439724418E-4	0.0	0.06943855041490746	0.0
72-73	5.271678592082255E-5	2.8994232256452404E-4	0.0	0.0726964477848143	0.0
74-75	5.271678592082255E-5	3.1102903693285304E-4	0.0	0.0752479402233821	0.0
76-77	5.271678592082255E-5	3.321157513011821E-4	0.0	0.0776887274115162	0.0
78-79	5.271678592082255E-5	3.373874298932643E-4	0.0	0.07927550266773295	0.0
80-81	5.271678592082255E-5	3.373874298932643E-4	0.0	0.07973413870524411	0.0
82-83	5.271678592082255E-5	3.4265910848534656E-4	0.0	0.08022440481430776	0.0
84-85	5.271678592082255E-5	3.637458228536756E-4	0.0	0.0804510869937673	0.0
86-87	5.271678592082255E-5	3.690175014457579E-4	0.0	0.0805407055298327	0.0
88	5.271678592082255E-5	4.1119093018241587E-4	0.0	0.0805407055298327	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	20395	0.0	48.133247	1
CTTATAC	8305	0.0	41.4882	1
GTATCAA	35665	0.0	36.451008	1
TATACAC	10650	0.0	33.941063	3
ATCAACG	43190	0.0	29.73812	3
TATCAAC	45385	0.0	28.44891	2
TCAACGC	45500	0.0	28.200462	4
ACGCAGA	45755	0.0	28.063997	7
TTATACA	12420	0.0	27.912657	2
CAACGCA	46000	0.0	27.886803	5
AACGCAG	46585	0.0	27.600142	6
ACACATC	14005	0.0	24.332846	6
CGCAGAG	53590	0.0	23.95358	8
ATACACA	16410	0.0	22.980803	4
TACACAT	15535	0.0	22.88894	5
ACATCTC	15970	0.0	21.5777	8
GCAGAGT	60090	0.0	21.229477	9
GTGGTAT	9545	0.0	20.906084	1
ACATGGG	41680	0.0	20.743141	3
GAGTACT	33380	0.0	20.683973	12-13
>>END_MODULE