FastQCFastQC Report
Fri 27 May 2016
SRR2139521_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139521_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10567614
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA148500.050.588131
GTATCAA260500.036.5650371
TCAACGC310300.030.1643244
ATCAACG315250.029.7044813
CAACGCA318250.029.3982625
TATCAAC325850.028.924972
AACGCAG327800.028.621366
ACGCAGA374450.025.0091927
CGCAGAG385800.024.2735528
GTACATG346450.021.7590481
TACATGG345200.021.3844592
GCAGAGT445750.021.009649
ACATGGG354400.020.3999863
GAGTACT247000.020.2505812-13
CTTATAC59950.019.3621431
CAGAGTA432650.019.18877210-11
AGAGTAC401600.018.82652310-11
GTGGTAT68650.018.7448731
CATGGGG249500.018.435144
GTACTTT273450.018.30022814-15
Produced by FastQC (version 0.11.5)