##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139521_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10567614
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.50586622486401	33.0	33.0	33.0	33.0	33.0
2	32.54276935171932	33.0	33.0	33.0	33.0	33.0
3	32.56233024786863	33.0	33.0	33.0	33.0	33.0
4	36.4694736200622	37.0	37.0	37.0	37.0	37.0
5	36.493963916547294	37.0	37.0	37.0	37.0	37.0
6	36.483038082200956	37.0	37.0	37.0	37.0	37.0
7	36.46096091322033	37.0	37.0	37.0	37.0	37.0
8	36.45342108445672	37.0	37.0	37.0	37.0	37.0
9	36.49395814419414	37.0	37.0	37.0	37.0	37.0
10-11	36.41494399776525	37.0	37.0	37.0	37.0	37.0
12-13	36.41716517087017	37.0	37.0	37.0	37.0	37.0
14-15	38.39495859708729	40.0	37.0	40.0	37.0	40.0
16-17	38.34756800352473	40.0	37.0	40.0	37.0	40.0
18-19	38.346502058080475	40.0	37.0	40.0	37.0	40.0
20-21	38.343977506387574	40.0	37.0	40.0	37.0	40.0
22-23	38.34473539461007	40.0	37.0	40.0	37.0	40.0
24-25	38.33025653710615	40.0	37.0	40.0	37.0	40.0
26-27	38.22017694237017	40.0	37.0	40.0	37.0	40.0
28-29	38.15321209897796	40.0	37.0	40.0	35.0	40.0
30-31	38.01512220025651	40.0	37.0	40.0	33.0	40.0
32-33	38.01751605319812	40.0	37.0	40.0	33.0	40.0
34-35	37.972804140781406	40.0	37.0	40.0	33.0	40.0
36-37	37.896869797289185	40.0	37.0	40.0	33.0	40.0
38-39	37.84231328285722	40.0	37.0	40.0	33.0	40.0
40-41	37.8455486695689	40.0	37.0	40.0	33.0	40.0
42-43	37.777523791849276	40.0	37.0	40.0	33.0	40.0
44-45	37.711773646668945	40.0	37.0	40.0	33.0	40.0
46-47	37.674381923022864	40.0	37.0	40.0	33.0	40.0
48-49	37.645786953642784	40.0	37.0	40.0	33.0	40.0
50-51	37.5592975913611	40.0	37.0	40.0	33.0	40.0
52-53	37.453748116051	40.0	37.0	40.0	33.0	40.0
54-55	37.2949850786098	40.0	37.0	40.0	33.0	40.0
56-57	37.188461682918266	38.5	37.0	40.0	33.0	40.0
58-59	37.023191401707024	37.0	37.0	40.0	33.0	40.0
60-61	36.9036221881005	37.0	37.0	40.0	33.0	40.0
62-63	36.735231055384105	37.0	37.0	40.0	33.0	40.0
64-65	36.55666395115662	37.0	37.0	40.0	33.0	40.0
66-67	36.32711450134263	37.0	37.0	40.0	33.0	40.0
68-69	36.10135151641519	37.0	37.0	40.0	33.0	40.0
70-71	35.8503787194949	37.0	35.0	37.0	33.0	40.0
72-73	35.643259412404504	37.0	33.0	37.0	33.0	40.0
74-75	35.42619241890485	37.0	33.0	37.0	33.0	40.0
76-77	33.7127031305958	35.0	33.0	37.0	27.0	37.0
78-79	34.93282552438209	37.0	33.0	37.0	33.0	37.0
80-81	35.01253569983621	37.0	33.0	37.0	33.0	37.0
82-83	34.86297997225702	37.0	33.0	37.0	33.0	37.0
84-85	34.72876250308708	37.0	33.0	37.0	33.0	37.0
86-87	34.612812858192015	37.0	33.0	37.0	33.0	37.0
88-89	34.44551340973521	37.0	33.0	37.0	30.0	37.0
90-91	34.45066618225335	37.0	33.0	37.0	33.0	37.0
92-93	34.38251786475738	37.0	33.0	37.0	33.0	37.0
94-95	34.35752581614297	37.0	33.0	37.0	33.0	37.0
96-97	34.36202525203057	37.0	33.0	37.0	33.0	37.0
98-99	34.36186375984144	37.0	33.0	37.0	33.0	37.0
100	34.37223890647578	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	3.0
18	0.0
19	21.0
20	93.0
21	448.0
22	1703.0
23	4929.0
24	11581.0
25	22436.0
26	38898.0
27	61523.0
28	90216.0
29	126506.0
30	170765.0
31	226786.0
32	300418.0
33	400285.0
34	548440.0
35	792147.0
36	1365409.0
37	3092186.0
38	3246015.0
39	66806.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.535392946790076	20.323963384733773	14.496545767095581	23.644097901380576
2	14.619127837182546	21.10668500950167	41.45024600633596	22.82394114697982
3	17.880346500165505	26.472730741300733	30.662503380611746	24.984419377922016
4	12.4729953232584	18.33703426336352	38.84317689877772	30.34679351460036
5	13.402911953445686	37.87041237501673	35.46041708185027	13.266258589687322
6	29.770801230478533	37.95348535277087	18.887786777617162	13.38792663913344
7	26.463286793026313	32.83178208439483	23.068925492547322	17.63600563003153
8	24.63581656180856	36.42715375485895	21.110933839937758	17.826095843394736
9	25.76155088192575	16.77591713473989	21.02002599830255	36.442505985031815
10-11	24.22297864769862	26.646405945920627	28.8457918785833	20.284823527797453
12-13	25.183359761565832	24.25521260099135	28.862814147338668	21.69861349010415
14-15	22.29888124822123	25.578916015664277	26.48866095426411	25.633541781850383
16-17	21.807581162597348	28.726172246639592	27.13991540569139	22.326331185071673
18-19	21.843544815319714	27.858161738307246	28.902011371724974	21.39628207464807
20-21	22.720837153874797	26.915165928645067	28.79590617439403	21.568090743086106
22-23	22.466594763286913	26.93332684742324	28.614797461920794	21.985280927369054
24-25	22.222838010232756	27.116330216872807	28.67134849607451	21.989483276819925
26-27	22.114764622171222	27.24039509427138	28.670845985382797	21.973994298174603
28-29	22.05324647184402	27.20306241215871	28.72071355245257	22.022977563544696
30-31	22.333392662927874	27.112441273774007	28.520074219663755	22.034091843634364
32-33	22.176565224111226	27.26044150467491	28.529133262927726	22.03386000828614
34-35	22.32526627181226	27.305947613005905	28.504882953781603	21.863903161400234
36-37	22.257269942055625	27.228568611002068	28.36239231040088	22.15176913654143
38-39	22.142025555458503	27.32880008519284	28.48416334530261	22.04501101404605
40-41	22.358793870090192	27.205516082445136	28.391592055237737	22.044097992226934
42-43	22.298404432614692	27.297056678147534	28.18042519479641	22.224113694441357
44-45	22.328831926680213	27.440517404136333	27.99858508331326	22.2320655858702
46-47	22.50133572850476	27.406935760468233	27.633201705972077	22.45852680505493
48-49	22.422463761864208	27.507232837340347	27.614094203747	22.456209197048445
50-51	22.404211869523742	27.649517231651338	27.52178352246063	22.424487376364294
52-53	22.573133762108675	27.56912646000238	27.44529703503527	22.412442742853678
54-55	22.528875462244333	27.497528470540093	27.44952952559421	22.52406654162136
56-57	22.56260387473382	27.638094044395846	27.358080711292597	22.44122136957774
58-59	22.44086831589849	27.698351552044652	27.444875448742245	22.415904683314608
60-61	22.583434927868986	27.726635924284604	27.33608031458393	22.35384883326248
62-63	22.527599620083556	27.830362333803933	27.25610654834871	22.385931497763803
64-65	22.545744567843098	27.69140609264665	27.272053777548443	22.49079556196181
66-67	22.6289436801358	27.6483959305517	27.162067466476238	22.560592922836264
68-69	22.512489983317202	27.64478075667936	27.263812438200397	22.57891682180304
70-71	22.552485251289724	27.462086061020546	27.320815184650186	22.664613503039543
72-73	22.619610507004133	27.512320751386653	27.236300618845643	22.63176812276357
74-75	22.501322145884274	27.660714836255494	27.19266549137797	22.645297526482263
76-77	22.61878113237071	27.546970217242006	27.16827997183417	22.66596867855311
78-79	22.552608010985608	27.52280903418141	27.147008376865784	22.7775745779672
80-81	22.55985218179093	27.591184849252574	27.159213855790586	22.68974911316591
82-83	22.60173950835112	27.578432541650184	27.228445978001865	22.591381971996835
84-85	22.67415802558719	27.549261737396325	27.139964183720167	22.63661605329632
86-87	22.561696326670322	27.5346424804649	27.237739402361882	22.665921790502896
88-89	22.61184817923678	27.536719357364316	27.193416343772387	22.658016119626517
90-91	22.622121534484986	27.628392587357176	27.15302907382601	22.59645680433183
92-93	22.655555842139776	27.548643248610095	27.203140184904008	22.59266072434612
94-95	22.512512108395956	27.550914807624473	27.27116828663726	22.66540479734231
96-97	22.606518961926593	27.676782219105995	27.28660276022606	22.430096058741356
98-99	23.762077744540992	29.546585769736723	27.474852329832373	19.21648415588991
100	22.630691311129038	30.61740979484885	21.27576443808114	25.476134455940976
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2401.0
1	2091.5
2	2698.5
3	3819.0
4	5086.0
5	8207.0
6	10831.0
7	12539.0
8	13537.5
9	13198.0
10	12081.0
11	11062.5
12	12308.0
13	16495.5
14	26342.0
15	40467.0
16	52365.5
17	59666.5
18	61743.0
19	58545.5
20	52115.0
21	45201.5
22	40529.0
23	40558.5
24	45771.0
25	56133.0
26	70510.0
27	87782.0
28	107701.0
29	131488.5
30	155976.0
31	179467.0
32	205716.0
33	232099.5
34	257523.5
35	281062.0
36	304641.0
37	326441.0
38	343895.0
39	360803.0
40	377323.0
41	390780.0
42	397708.0
43	405829.5
44	415267.0
45	422993.5
46	427295.0
47	426888.5
48	430384.5
49	430353.5
50	422709.0
51	411891.0
52	398251.0
53	378228.5
54	352793.5
55	327151.0
56	300742.5
57	272638.0
58	242886.0
59	210861.0
60	177038.0
61	144923.0
62	115198.5
63	88996.5
64	66355.5
65	48969.5
66	36962.0
67	28351.5
68	22064.0
69	16396.0
70	11796.5
71	8704.0
72	6110.0
73	3990.0
74	2629.5
75	1697.5
76	1114.0
77	692.5
78	453.0
79	334.0
80	249.0
81	173.0
82	116.5
83	78.5
84	54.0
85	38.0
86	25.5
87	18.0
88	11.0
89	5.0
90	3.0
91	2.0
92	2.0
93	1.5
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	1.7033173240430622E-4
7	0.0
8	0.0
9	9.462874022461456E-6
10-11	0.0034918005142882775
12-13	9.462874022461456E-6
14-15	0.0024272271867613634
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	156.0
20-21	662.0
22-23	1413.0
24-25	2962.0
26-27	3921.0
28-29	6776.0
30-31	14345.0
32-33	18240.0
34-35	25707.0
36-37	35765.0
38-39	44093.0
40-41	48861.0
42-43	60770.0
44-45	74050.0
46-47	71427.0
48-49	63108.0
50-51	55496.0
52-53	54337.0
54-55	60047.0
56-57	64740.0
58-59	65629.0
60-61	66830.0
62-63	66586.0
64-65	71441.0
66-67	76322.0
68-69	74366.0
70-71	73707.0
72-73	71143.0
74-75	74212.0
76-77	73167.0
78-79	80090.0
80-81	82330.0
82-83	81275.0
84-85	82236.0
86-87	88598.0
88-89	94622.0
90-91	101593.0
92-93	108883.0
94-95	133542.0
96-97	381560.0
98-99	674577.0
100-101	7238029.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	82.860815893851
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.2547638459531	73.12861738794817
2	8.012187349773818	13.277927617933008
3	2.0157730332817416	5.01085794583644
4	0.7813617713979575	2.5897709554519786
5	0.37005188175492654	1.5331400422634043
6	0.19212310735523516	0.9551686456510028
7	0.10974823480827461	0.6365679795386502
8	0.06968933987499443	0.46196124489167384
9	0.045958661610901744	0.3427354978621855
>10	0.14506989650118748	1.8220719924513387
>50	0.0025023155900707104	0.13810278223326236
>100	7.503068797502965E-4	0.09174895167140537
>500	2.025521802281392E-5	0.01132895626747783
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	9.462874022461456E-6
2	0.0	1.8925748044922913E-5	0.0	0.0	9.462874022461456E-6
3	0.0	1.8925748044922913E-5	0.0	0.0	9.462874022461456E-6
4	0.0	7.570299217969165E-5	0.0	9.462874022461456E-6	9.462874022461456E-6
5	0.0	7.570299217969165E-5	0.0	9.462874022461456E-6	9.462874022461456E-6
6	0.0	7.570299217969165E-5	0.0	9.462874022461456E-6	9.462874022461456E-6
7	0.0	7.570299217969165E-5	0.0	3.7851496089845826E-5	9.462874022461456E-6
8	0.0	1.0409161424707602E-4	0.0	3.7851496089845826E-5	9.462874022461456E-6
9	0.0	1.7979460642676768E-4	0.0	5.677724413476874E-5	9.462874022461456E-6
10-11	4.731437011230728E-6	1.7979460642676768E-4	0.0	1.0882305125830675E-4	9.462874022461456E-6
12-13	9.462874022461456E-6	1.8452604343799838E-4	0.0	1.3248023631446038E-4	3.7851496089845826E-5
14-15	9.462874022461456E-6	3.170062797524588E-4	0.0	1.3248023631446038E-4	4.7314370112307285E-5
16-17	2.838862206738437E-5	3.501263388310739E-4	0.0	1.3248023631446038E-4	5.677724413476874E-5
18-19	3.7851496089845826E-5	3.6432064986476607E-4	0.0	1.372116733256911E-4	5.677724413476874E-5
20-21	3.7851496089845826E-5	3.879778349209197E-4	0.0	1.4194311033692184E-4	7.570299217969165E-5
22-23	5.204580712353801E-5	4.3056076802199623E-4	0.0	1.656002953930755E-4	7.570299217969165E-5
24-25	7.570299217969165E-5	4.7314370112307283E-4	0.0	1.7033173240430622E-4	7.570299217969165E-5
26-27	7.570299217969165E-5	4.826065751455343E-4	0.0	1.9398891746045984E-4	9.462874022461456E-5
28-29	7.570299217969165E-5	4.87338012156765E-4	0.0	2.6496047262892077E-4	9.462874022461456E-5
30-31	9.462874022461456E-5	6.387439965161482E-4	0.0	6.292811224936868E-4	9.936017723584529E-5
32-33	9.462874022461456E-5	6.482068705386098E-4	0.0	0.0014714769104927565	1.0409161424707602E-4
34-35	9.462874022461456E-5	7.99612854897993E-4	0.0	0.00246981011986244	1.3248023631446038E-4
36-37	1.1355448826953748E-4	9.084359061562997E-4	0.0	0.004102155888737041	1.3248023631446038E-4
38-39	1.1355448826953748E-4	9.510188392573764E-4	0.0	0.006723371992958865	1.4194311033692184E-4
40-41	1.1355448826953748E-4	0.0011355448826953747	0.0	0.009566965636708533	1.4194311033692184E-4
42-43	1.1355448826953748E-4	0.0012112478748750664	0.0	0.012325393414256047	1.5613742137061403E-4
44-45	1.1355448826953748E-4	0.0013153394891221425	0.0	0.015433947530634635	1.7033173240430622E-4
46-47	1.1355448826953748E-4	0.0013863110442906032	0.0	0.018807462119642147	1.7506316941553695E-4
48-49	1.2301736229199892E-4	0.0014383568514141415	0.0	0.02237023418909888	1.9872035447169057E-4
50-51	1.4667454734815257E-4	0.0017127801980655237	0.0	0.025847840392353466	1.9872035447169057E-4
52-53	1.5613742137061403E-4	0.001741168820132908	0.0	0.029273400788484515	2.0818322849415203E-4
54-55	1.7506316941553692E-4	0.0017695574422002922	0.0	0.03282670998391879	2.176461025166135E-4
56-57	1.8925748044922911E-4	0.0017979460642676766	0.0	0.03646518504555522	2.365718505615364E-4
58-59	2.0818322849415203E-4	0.0017979460642676766	0.0	0.04033550052074196	2.6022903561769006E-4
60-61	2.176461025166135E-4	0.0018405289973687533	0.0	0.04420108455891746	2.6496047262892077E-4
62-63	2.2237753952784422E-4	0.0018689176194361377	0.0	0.0479483826718122	2.6496047262892077E-4
64-65	2.2710897653907495E-4	0.002105489469997674	0.0	0.051799772398954017	2.6496047262892077E-4
66-67	2.3184041355030568E-4	0.0021622667141324428	0.0	0.05584988248056751	2.6496047262892077E-4
68-69	2.3657185056153641E-4	0.0022143125212559806	0.0	0.05988106681413609	2.6969190964015147E-4
70-71	2.4130328757276714E-4	0.0022521640173458262	0.0	0.06368987360817682	2.744233466513822E-4
72-73	2.4603472458399785E-4	0.0022758212024019805	0.0	0.06667067892525219	3.028119687187666E-4
74-75	2.4603472458399785E-4	0.002346792757570441	0.0	0.06943383813981094	3.1227484274122806E-4
76-77	2.4603472458399785E-4	0.002436690060783825	0.0	0.07196042550380814	3.1227484274122806E-4
78-79	2.4603472458399785E-4	0.002507661615952286	0.0	0.07364954851681751	3.1227484274122806E-4
80-81	2.4603472458399785E-4	0.0025218559269859784	0.0	0.07418893233609783	3.170062797524588E-4
82-83	2.4603472458399785E-4	0.0025455131120421318	0.0	0.07461476166710859	3.217377167636895E-4
84-85	2.4603472458399785E-4	0.002592827482154439	0.0	0.07480875058456904	3.217377167636895E-4
86-87	2.4603472458399785E-4	0.002611753230199362	0.0	0.07491284219881611	3.217377167636895E-4
88	2.4603472458399785E-4	0.002696919096401515	0.0	0.07491757363582735	3.31200590786151E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	14850	0.0	50.58813	1
GTATCAA	26050	0.0	36.565037	1
TCAACGC	31030	0.0	30.164324	4
ATCAACG	31525	0.0	29.704481	3
CAACGCA	31825	0.0	29.398262	5
TATCAAC	32585	0.0	28.92497	2
AACGCAG	32780	0.0	28.62136	6
ACGCAGA	37445	0.0	25.009192	7
CGCAGAG	38580	0.0	24.273552	8
GTACATG	34645	0.0	21.759048	1
TACATGG	34520	0.0	21.384459	2
GCAGAGT	44575	0.0	21.00964	9
ACATGGG	35440	0.0	20.399986	3
GAGTACT	24700	0.0	20.25058	12-13
CTTATAC	5995	0.0	19.362143	1
CAGAGTA	43265	0.0	19.188772	10-11
AGAGTAC	40160	0.0	18.826523	10-11
GTGGTAT	6865	0.0	18.744873	1
CATGGGG	24950	0.0	18.43514	4
GTACTTT	27345	0.0	18.300228	14-15
>>END_MODULE