FastQCFastQC Report
Fri 27 May 2016
SRR2139520_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139520_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13015852
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA178250.050.1722831
GTATCAA324400.037.265781
TCAACGC387600.030.5245154
ATCAACG392800.030.185723
CAACGCA399800.029.6266655
TATCAAC405000.029.531092
AACGCAG409900.028.9490836
ACGCAGA466750.025.3862637
CGCAGAG476300.024.850388
GTGGTAT87350.021.5984691
GCAGAGT550400.021.3570829
GTACATG427350.021.2690451
TACATGG432300.020.5582732
GAGTACT301350.020.3227112-13
TGGTATC88400.019.9320932
ACATGGG441100.019.5051063
CTTATAC78650.019.0698831
CAGAGTA540150.018.96536810-11
AGAGTAC500950.018.39033510-11
GTACTTT339400.017.9498214-15
Produced by FastQC (version 0.11.5)