##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139520_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	13015852
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.5369517877124	33.0	33.0	33.0	33.0	33.0
2	32.573012354473605	33.0	33.0	33.0	33.0	33.0
3	32.597211769156566	33.0	33.0	33.0	33.0	33.0
4	36.5115666650174	37.0	37.0	37.0	37.0	37.0
5	36.536960162116166	37.0	37.0	37.0	37.0	37.0
6	36.522864580820375	37.0	37.0	37.0	37.0	37.0
7	36.50271361413759	37.0	37.0	37.0	37.0	37.0
8	36.49354464079647	37.0	37.0	37.0	37.0	37.0
9	36.52916290074595	37.0	37.0	37.0	37.0	37.0
10-11	36.45484314050283	37.0	37.0	37.0	37.0	37.0
12-13	36.45800797366165	37.0	37.0	37.0	37.0	37.0
14-15	38.4731195468418	40.0	37.0	40.0	37.0	40.0
16-17	38.428924168775126	40.0	37.0	40.0	37.0	40.0
18-19	38.43348080479096	40.0	37.0	40.0	37.0	40.0
20-21	38.43131753779713	40.0	37.0	40.0	37.0	40.0
22-23	38.43416986257131	40.0	37.0	40.0	37.0	40.0
24-25	38.42286021744516	40.0	37.0	40.0	37.0	40.0
26-27	38.31673107345446	40.0	37.0	40.0	37.0	40.0
28-29	38.251947027271825	40.0	37.0	40.0	37.0	40.0
30-31	38.11296785650268	40.0	37.0	40.0	37.0	40.0
32-33	38.121721940181374	40.0	37.0	40.0	37.0	40.0
34-35	38.08522870658295	40.0	37.0	40.0	33.0	40.0
36-37	38.004344727500296	40.0	37.0	40.0	33.0	40.0
38-39	37.95263340505093	40.0	37.0	40.0	33.0	40.0
40-41	37.9640867134203	40.0	37.0	40.0	33.0	40.0
42-43	37.90143918124606	40.0	37.0	40.0	33.0	40.0
44-45	37.84439439514995	40.0	37.0	40.0	33.0	40.0
46-47	37.81010429030016	40.0	37.0	40.0	33.0	40.0
48-49	37.785514824510585	40.0	37.0	40.0	33.0	40.0
50-51	37.70600610695498	40.0	37.0	40.0	33.0	40.0
52-53	37.605147834625726	40.0	37.0	40.0	33.0	40.0
54-55	37.45959551464502	40.0	37.0	40.0	33.0	40.0
56-57	37.35863783747568	40.0	37.0	40.0	33.0	40.0
58-59	37.19707791121998	37.0	37.0	40.0	33.0	40.0
60-61	37.08328503485477	37.0	37.0	40.0	33.0	40.0
62-63	36.91805324854577	37.0	37.0	40.0	33.0	40.0
64-65	36.74313033509231	37.0	37.0	40.0	33.0	40.0
66-67	36.51837003168228	37.0	37.0	40.0	33.0	40.0
68-69	36.292492031504246	37.0	37.0	40.0	33.0	40.0
70-71	36.04109471792994	37.0	37.0	38.5	33.0	40.0
72-73	35.83398469281668	37.0	35.0	37.0	33.0	40.0
74-75	35.613637575985145	37.0	33.0	37.0	33.0	40.0
76-77	33.91653431678651	35.0	33.0	37.0	27.0	37.0
78-79	35.10068100438065	37.0	33.0	37.0	33.0	37.0
80-81	35.164226887210674	37.0	33.0	37.0	33.0	37.0
82-83	35.011099340052546	37.0	33.0	37.0	33.0	37.0
84-85	34.879406091321755	37.0	33.0	37.0	33.0	37.0
86-87	34.7651080135558	37.0	33.0	37.0	33.0	37.0
88-89	34.60123063607652	37.0	33.0	37.0	33.0	37.0
90-91	34.60513441604084	37.0	33.0	37.0	33.0	37.0
92-93	34.53608808500178	37.0	33.0	37.0	33.0	37.0
94-95	34.511159670361266	37.0	33.0	37.0	33.0	37.0
96-97	34.51457200693824	37.0	33.0	37.0	33.0	37.0
98-99	34.49794464934541	37.0	33.0	37.0	33.0	37.0
100	34.51512882906228	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	0.0
18	3.0
19	22.0
20	85.0
21	469.0
22	1726.0
23	4924.0
24	11799.0
25	23593.0
26	40954.0
27	66159.0
28	98618.0
29	139519.0
30	190375.0
31	256321.0
32	341393.0
33	460705.0
34	634439.0
35	920313.0
36	1588009.0
37	3731165.0
38	4397074.0
39	108186.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.594680087020045	20.320844152192265	14.474726664070857	23.609749096716833
2	14.51521575383617	21.149771832070616	41.628738556646155	22.70627385744706
3	17.769555154745152	26.592312205148	30.725164975754183	24.912967664352667
4	12.33641869929068	18.4511624748038	39.11684767159307	30.095571154312452
5	13.218727440969674	37.983598768639965	35.63030679820268	13.167366992187679
6	29.550524246244763	38.15693659182703	19.05530419496874	13.237234966959463
7	26.380094057615285	32.91416497360296	23.209337352637384	17.496403616144377
8	24.53402205249414	36.53056288593324	21.230550255181143	17.70486480639147
9	25.776024940666574	16.822226990766872	21.092681531157666	36.30906653740888
10-11	24.083365580459997	26.779443060845402	28.9996025084647	20.1375888502299
12-13	25.13691482632998	24.378758279090164	28.964690927732523	21.519635966847336
14-15	22.226101776278302	25.732743996248175	26.65092034683495	25.390233880638576
16-17	21.697388691881255	28.86427642231949	27.274737758235112	22.16359712756414
18-19	21.734147714648262	28.019629448767546	29.027608027503693	21.218614809080496
20-21	22.62327399049395	27.10665184638714	28.927757591161125	21.342316571957788
22-23	22.3415700013047	27.100739898602217	28.749971378047505	21.807718722045575
24-25	22.090211072843594	27.275946352517455	28.799524066650957	21.834318507987994
26-27	22.001136413083003	27.46432199528983	28.74215246878093	21.792389122846238
28-29	21.916151531334737	27.438422287026665	28.79991167295643	21.845514508682164
30-31	22.308486156295654	27.31739488692866	28.64676633805847	21.727352618717223
32-33	21.93345866670718	27.55142085832874	28.618511485863674	21.896608989100407
34-35	22.146650733123824	27.541398537817663	28.61670400414507	21.695246724913442
36-37	22.122277174078448	27.488782448610174	28.504616608547533	21.884323768763846
38-39	21.98146654554275	27.5068428208453	28.65356545668945	21.8581251769225
40-41	22.2210771691181	27.371004840872963	28.503233089198176	21.904684900810754
42-43	22.144917110808045	27.47525959416668	28.29025203314859	22.08957126187669
44-45	22.199998151080013	27.62282492517131	28.09812829385988	22.079048629888792
46-47	22.32744653052418	27.597251167887922	27.758035326578867	22.31726697500903
48-49	22.251078832045064	27.671284629578878	27.776487443388092	22.301149094987963
50-51	22.259594430018847	27.80105889186068	27.6740277836011	22.265318894519368
52-53	22.39983337733637	27.738318174367855	27.593852431797742	22.26799601649803
54-55	22.40710018547995	27.674165911450565	27.559036024641788	22.3596978784277
56-57	22.407117386890583	27.81854216946471	27.469797279045803	22.304543164598904
58-59	22.31177078158209	27.84381991576808	27.581638462931508	22.262770839718325
60-61	22.45832494607196	27.89685056387458	27.43019239714185	22.214632092911607
62-63	22.356962156644947	27.98893463093235	27.416894478344155	22.237208734078546
64-65	22.373337245884727	27.85264639987294	27.39594673538791	22.378069618854425
66-67	22.43476440739594	27.842055688565253	27.30065502631003	22.42252487772878
68-69	22.380300892069087	27.84736026014436	27.398848108767375	22.37349073901917
70-71	22.376692356111487	27.647593431534574	27.45696636182296	22.518747850530982
72-73	22.450478030384	27.706392722473304	27.355059843976527	22.488069403166165
74-75	22.374832418423072	27.791669717261485	27.300986569069085	22.532511295246355
76-77	22.423838736052	27.73096639222019	27.310439593890774	22.53475527783703
78-79	22.42295219518465	27.713347695158298	27.270632470995764	22.59306763866129
80-81	22.415569199498382	27.824073501733903	27.24259716629267	22.51776013247504
82-83	22.46287465065778	27.748850789485914	27.339380792614897	22.448893767241408
84-85	22.51474771377622	27.75116584755758	27.25091083305363	22.48317560561257
86-87	22.408267047553377	27.758737125656623	27.32784324794323	22.50515257884677
88-89	22.46374584109955	27.6903958606731	27.323525874240516	22.52233242398683
90-91	22.459177409275206	27.786909513838133	27.288917001942448	22.46499607494422
92-93	22.536371713936525	27.714821623684927	27.308947002513506	22.439859659865046
94-95	22.356300449704175	27.74011092033114	27.392359456128844	22.51122917383584
96-97	22.484133521965056	27.856688782914567	27.388086642782376	22.271091052338
98-99	23.597668724195678	29.72424605990599	27.631424752943833	19.046660462954502
100	22.46987537201092	30.758946161133675	21.44277186763686	25.32840659921854
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2912.0
1	2647.5
2	3549.5
3	4961.0
4	6544.5
5	10368.0
6	13487.0
7	15350.5
8	16496.0
9	16321.0
10	15290.5
11	14211.5
12	15585.5
13	20761.0
14	33172.5
15	50666.5
16	65535.5
17	75084.5
18	77848.5
19	73656.5
20	65749.0
21	57168.0
22	51208.0
23	51162.0
24	57632.0
25	71014.0
26	89772.5
27	111787.0
28	137701.0
29	167116.0
30	198171.5
31	229921.0
32	265313.0
33	300709.5
34	334906.0
35	367665.0
36	400355.0
37	429262.0
38	450136.5
39	470031.5
40	489339.0
41	503133.5
42	510738.0
43	519531.5
44	527882.5
45	535060.5
46	540881.0
47	541965.0
48	538249.0
49	531349.0
50	522055.5
51	505724.0
52	483540.0
53	455723.0
54	424785.0
55	394194.0
56	361560.5
57	325513.0
58	287919.0
59	249786.0
60	209155.5
61	169439.5
62	134218.0
63	103513.5
64	77145.0
65	57409.5
66	43736.0
67	33595.0
68	25773.5
69	19044.5
70	13670.5
71	9684.5
72	6656.0
73	4565.5
74	3111.5
75	2025.5
76	1298.5
77	818.5
78	517.0
79	328.0
80	206.5
81	142.5
82	119.5
83	103.0
84	74.0
85	48.0
86	36.0
87	25.5
88	20.0
89	18.0
90	14.0
91	11.0
92	8.0
93	5.0
94	2.0
95	1.0
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	1.6902466315689513E-4
7	0.0
8	0.0
9	7.682939234404326E-6
10-11	0.003691652302131278
12-13	3.457322655481946E-5
14-15	0.002489272311947001
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	197.0
20-21	810.0
22-23	1683.0
24-25	4286.0
26-27	5324.0
28-29	9093.0
30-31	20875.0
32-33	26522.0
34-35	37800.0
36-37	55005.0
38-39	66931.0
40-41	72250.0
42-43	87385.0
44-45	106037.0
46-47	102305.0
48-49	91819.0
50-51	81468.0
52-53	79919.0
54-55	87344.0
56-57	92570.0
58-59	92158.0
60-61	93191.0
62-63	91840.0
64-65	97057.0
66-67	103954.0
68-69	100600.0
70-71	100322.0
72-73	96374.0
74-75	99497.0
76-77	98961.0
78-79	105424.0
80-81	108412.0
82-83	105903.0
84-85	106842.0
86-87	113498.0
88-89	120386.0
90-91	127853.0
92-93	134546.0
94-95	163427.0
96-97	463403.0
98-99	802889.0
100-101	8659692.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.07725517194538
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.97604661146433	74.80861775142992
2	7.744283953568705	13.022362761763961
3	1.8407040121778067	4.642840227836914
4	0.648929279538993	2.182407704973864
5	0.3070651442193156	1.2908597242468802
6	0.15577949193128152	0.7858507256197418
7	0.09663807625142824	0.5687544937422074
8	0.06453668498173529	0.4340853864928651
9	0.0364203584045012	0.2755911390326058
>10	0.12510830405069664	1.643008756046068
>50	0.0034407688272045144	0.19351448871217655
>100	0.0010156672689921847	0.13679383844641513
>500	3.164731507854447E-5	0.015313001656357534
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	7.682939234404326E-6	0.0	2.3048817703212975E-5	0.0
3	0.0	1.536587846880865E-5	0.0	3.07317569376173E-5	0.0
4	0.0	6.914645310963893E-5	0.0	3.07317569376173E-5	0.0
5	0.0	6.914645310963893E-5	0.0	3.07317569376173E-5	0.0
6	7.682939234404325E-5	6.914645310963893E-5	0.0	3.07317569376173E-5	0.0
7	7.682939234404325E-5	6.914645310963893E-5	0.0	3.07317569376173E-5	0.0
8	7.682939234404325E-5	7.682939234404325E-5	0.0	3.841469617202162E-5	0.0
9	7.682939234404325E-5	1.1524408851606488E-4	0.0	6.914645310963893E-5	0.0
10-11	7.682939234404325E-5	1.1524408851606488E-4	0.0	1.1908555813326704E-4	0.0
12-13	7.682939234404325E-5	1.1908555813326704E-4	0.0	1.3445143660207568E-4	0.0
14-15	8.451233157844758E-5	1.4213437583648003E-4	0.0	1.5750025430528867E-4	0.0
16-17	1.4213437583648003E-4	1.4597584545368217E-4	0.0	1.6902466315689513E-4	0.0
18-19	1.5750025430528867E-4	1.65183193539693E-4	0.0	1.728661327740973E-4	0.0
20-21	1.613417239224908E-4	1.843905416257038E-4	0.0	1.9975642009451244E-4	0.0
22-23	1.9207348086010812E-4	2.1512229856332108E-4	0.0	2.458540555009384E-4	0.0
24-25	2.3432964664933193E-4	2.4201258588373625E-4	0.0	2.765858124385557E-4	0.0
26-27	2.6121993396974706E-4	2.650614035869492E-4	0.0	3.2652491746218383E-4	0.0
28-29	2.6121993396974706E-4	2.8426875167296005E-4	0.0	4.5329341482985516E-4	0.0
30-31	2.650614035869492E-4	3.1500050861057733E-4	0.0	9.411600562145299E-4	7.682939234404326E-6
32-33	2.881102212901622E-4	3.188419782277795E-4	0.0	0.0021896376818052324	7.682939234404326E-6
34-35	3.380493263137903E-4	3.803054921030141E-4	0.0	0.003714701119834491	7.682939234404326E-6
36-37	3.687810832514076E-4	4.379275363610465E-4	0.0	0.006046473177476204	7.682939234404326E-6
38-39	3.687810832514076E-4	4.5329341482985516E-4	0.0	0.009707393722669863	7.682939234404326E-6
40-41	3.764640224858119E-4	5.147569287050897E-4	0.0	0.013618009792981665	7.682939234404326E-6
42-43	3.764640224858119E-4	5.570130944943135E-4	0.0	0.017624662603723522	7.682939234404326E-6
44-45	3.918299009546206E-4	6.338424868383569E-4	0.0	0.022172962630490883	7.682939234404326E-6
46-47	4.225616578922379E-4	6.760986526275805E-4	0.0	0.027274434282135353	7.682939234404326E-6
48-49	4.225616578922379E-4	6.837815918619848E-4	0.0	0.03229907654143578	7.682939234404326E-6
50-51	4.225616578922379E-4	7.990256803780498E-4	0.0	0.037481219055041495	7.682939234404326E-6
52-53	4.225616578922379E-4	8.143915588468585E-4	0.0	0.04268256891673323	7.682939234404326E-6
54-55	4.225616578922379E-4	8.143915588468585E-4	0.0	0.04772257705450246	7.682939234404326E-6
56-57	4.2640312750944E-4	8.143915588468585E-4	0.0	0.05215947446237096	7.682939234404326E-6
58-59	4.3408606674384437E-4	8.182330284640606E-4	0.0	0.05660021333985666	7.682939234404326E-6
60-61	4.379275363610465E-4	8.297574373156671E-4	0.0	0.061148513366624024	7.682939234404326E-6
62-63	4.4561047559545086E-4	8.374403765500715E-4	0.0	0.06591961863118911	7.682939234404326E-6
64-65	4.4561047559545086E-4	9.065868296597103E-4	0.0	0.0705216992325973	7.682939234404326E-6
66-67	4.609763540642595E-4	9.411600562145298E-4	0.0	0.07519292628711513	7.682939234404326E-6
68-69	4.7250076291586595E-4	9.603674043005406E-4	0.0	0.08004854388325866	7.682939234404326E-6
70-71	4.801837021502703E-4	9.834162220037535E-4	0.0	0.08409745285978974	7.682939234404326E-6
72-73	5.032325198534833E-4	9.910991612381579E-4	0.0	0.08787361749349946	7.682939234404326E-6
74-75	5.070739894706854E-4	0.001017989448558573	0.0	0.09082386615951073	7.682939234404326E-6
76-77	5.070739894706854E-4	0.0010756114928166054	0.0	0.09365887073700593	7.682939234404326E-6
78-79	5.147569287050897E-4	0.0010986603105198184	0.0	0.0955757640759898	7.682939234404326E-6
80-81	5.147569287050897E-4	0.001114026188988627	0.0	0.09625570419823459	7.682939234404326E-6
82-83	5.224398679394941E-4	0.0011255505978402336	0.0	0.09665905850804081	7.682939234404326E-6
84-85	5.378057464083027E-4	0.0011293920674574358	0.0	0.0968204002319633	7.682939234404326E-6
86-87	5.454886856427071E-4	0.0011447579459262444	0.0	0.09687802227622133	7.682939234404326E-6
88	5.608545641115157E-4	0.001183172642098266	0.0	0.09688186374583854	7.682939234404326E-6
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	17825	0.0	50.172283	1
GTATCAA	32440	0.0	37.26578	1
TCAACGC	38760	0.0	30.524515	4
ATCAACG	39280	0.0	30.18572	3
CAACGCA	39980	0.0	29.626665	5
TATCAAC	40500	0.0	29.53109	2
AACGCAG	40990	0.0	28.949083	6
ACGCAGA	46675	0.0	25.386263	7
CGCAGAG	47630	0.0	24.85038	8
GTGGTAT	8735	0.0	21.598469	1
GCAGAGT	55040	0.0	21.357082	9
GTACATG	42735	0.0	21.269045	1
TACATGG	43230	0.0	20.558273	2
GAGTACT	30135	0.0	20.32271	12-13
TGGTATC	8840	0.0	19.932093	2
ACATGGG	44110	0.0	19.505106	3
CTTATAC	7865	0.0	19.069883	1
CAGAGTA	54015	0.0	18.965368	10-11
AGAGTAC	50095	0.0	18.390335	10-11
GTACTTT	33940	0.0	17.94982	14-15
>>END_MODULE