FastQCFastQC Report
Fri 27 May 2016
SRR2139519_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139519_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12478785
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA164200.050.654511
GTATCAA295200.037.293541
TCAACGC355450.030.326134
ATCAACG361700.029.8498043
CAACGCA367800.029.3450745
TATCAAC373750.029.0149862
AACGCAG376600.028.7054086
ACGCAGA428850.025.1776927
CGCAGAG443650.024.3285888
GTACATG396850.021.3955921
GCAGAGT508800.021.12889
TACATGG403400.020.500232
GAGTACT280500.020.38218912-13
CTTATAC72350.019.8292261
GTGGTAT85750.019.7632731
TGGTATC79400.019.6516152
ACATGGG410800.019.635063
CAGAGTA499500.019.00974310-11
AGAGTAC463300.018.69865210-11
GTACTTT313750.018.2018814-15
Produced by FastQC (version 0.11.5)