FastQCFastQC Report
Fri 27 May 2016
SRR2139518_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139518_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13959300
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA200800.049.9578861
GTATCAA357950.036.518141
TCAACGC430950.029.6510284
ATCAACG432850.029.5805233
CAACGCA442800.028.908465
TATCAAC452350.028.496292
AACGCAG452750.028.2922236
CTTATAC112450.026.0368711
ACGCAGA511850.024.9833167
CGCAGAG523850.024.4358338
TATACAC153050.022.572273
GCAGAGT606750.020.9984289
GTGGTAT99000.020.7630121
TGGTATC95350.020.7426092
GTACATG447900.020.3817981
GAGTACT346150.020.1424612-13
TACATGG447900.019.9286542
TTATACA159650.019.7727472
CAGAGTA587800.019.22857910-11
ACATGGG458400.019.019993
Produced by FastQC (version 0.11.5)