FastQCFastQC Report
Fri 27 May 2016
SRR2139517_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139517_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13162308
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA191850.049.03631
GTATCAA345350.036.888091
TCAACGC416050.030.0633344
ATCAACG419050.029.8274153
CAACGCA425950.029.3559765
TATCAAC432100.029.1349772
AACGCAG437350.028.688836
ACGCAGA499200.025.1427357
CTTATAC151300.024.8544161
CGCAGAG512250.024.5023888
TATACAC198200.021.6124883
GCAGAGT585950.021.2674759
GTACATG462700.020.5544051
GAGTACT311500.020.4558212-13
GTGGTAT90450.019.9105661
TACATGG469650.019.8211292
TTATACA202900.019.7804742
ACATGGG464750.019.5134413
CAGAGTA572300.018.95727510-11
AGAGTAC535150.018.60655210-11
Produced by FastQC (version 0.11.5)