##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139517_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	13162308
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.52039064881326	33.0	33.0	33.0	33.0	33.0
2	32.551115275527664	33.0	33.0	33.0	33.0	33.0
3	32.56850029645257	33.0	33.0	33.0	33.0	33.0
4	36.47687183737077	37.0	37.0	37.0	37.0	37.0
5	36.50255502302484	37.0	37.0	37.0	37.0	37.0
6	36.49365863494457	37.0	37.0	37.0	37.0	37.0
7	36.470230676869136	37.0	37.0	37.0	37.0	37.0
8	36.46415491872702	37.0	37.0	37.0	37.0	37.0
9	36.50230179995788	37.0	37.0	37.0	37.0	37.0
10-11	36.42217348963419	37.0	37.0	37.0	37.0	37.0
12-13	36.42455950734476	37.0	37.0	37.0	37.0	37.0
14-15	38.40202774467821	40.0	37.0	40.0	37.0	40.0
16-17	38.354803769977124	40.0	37.0	40.0	37.0	40.0
18-19	38.36194055024393	40.0	37.0	40.0	37.0	40.0
20-21	38.35938490533884	40.0	37.0	40.0	37.0	40.0
22-23	38.360892199401576	40.0	37.0	40.0	37.0	40.0
24-25	38.345867554571754	40.0	37.0	40.0	37.0	40.0
26-27	38.2319128890002	40.0	37.0	40.0	37.0	40.0
28-29	38.16493700550102	40.0	37.0	40.0	37.0	40.0
30-31	38.01798824697863	40.0	37.0	40.0	33.0	40.0
32-33	38.02520764829596	40.0	37.0	40.0	33.0	40.0
34-35	37.98590537170317	40.0	37.0	40.0	33.0	40.0
36-37	37.900791971881596	40.0	37.0	40.0	33.0	40.0
38-39	37.84600518208144	40.0	37.0	40.0	33.0	40.0
40-41	37.85648044541044	40.0	37.0	40.0	33.0	40.0
42-43	37.79592181158434	40.0	37.0	40.0	33.0	40.0
44-45	37.73454086280245	40.0	37.0	40.0	33.0	40.0
46-47	37.700949746977585	40.0	37.0	40.0	33.0	40.0
48-49	37.68210945706848	40.0	37.0	40.0	33.0	40.0
50-51	37.58805520506802	40.0	37.0	40.0	33.0	40.0
52-53	37.488801841269336	40.0	37.0	40.0	33.0	40.0
54-55	37.34049604318234	40.0	37.0	40.0	33.0	40.0
56-57	37.231976045891045	38.5	37.0	40.0	33.0	40.0
58-59	37.0707029803296	37.0	37.0	40.0	33.0	40.0
60-61	36.95605096045807	37.0	37.0	40.0	33.0	40.0
62-63	36.797838327844445	37.0	37.0	40.0	33.0	40.0
64-65	36.62757798047281	37.0	37.0	40.0	33.0	40.0
66-67	36.406601803587975	37.0	37.0	40.0	33.0	40.0
68-69	36.18178667165651	37.0	37.0	40.0	33.0	40.0
70-71	35.934141901710774	37.0	37.0	38.5	33.0	40.0
72-73	35.729220617629764	37.0	33.0	37.0	33.0	40.0
74-75	35.51036213458224	37.0	33.0	37.0	33.0	40.0
76-77	33.812976768639345	35.0	33.0	37.0	27.0	37.0
78-79	35.00428832586296	37.0	33.0	37.0	33.0	37.0
80-81	35.072686288622755	37.0	33.0	37.0	33.0	37.0
82-83	34.91947607692775	37.0	33.0	37.0	33.0	37.0
84-85	34.78958180257902	37.0	33.0	37.0	33.0	37.0
86-87	34.675941850766804	37.0	33.0	37.0	33.0	37.0
88-89	34.511473209608575	37.0	33.0	37.0	33.0	37.0
90-91	34.519148799612694	37.0	33.0	37.0	33.0	37.0
92-93	34.45271403502181	37.0	33.0	37.0	33.0	37.0
94-95	34.42976449040602	37.0	33.0	37.0	33.0	37.0
96-97	34.436394534523146	37.0	33.0	37.0	33.0	37.0
98-99	34.43505004280235	37.0	33.0	37.0	33.0	37.0
100	34.453321649126885	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	4.0
19	12.0
20	116.0
21	609.0
22	2185.0
23	6057.0
24	13862.0
25	27320.0
26	47200.0
27	74207.0
28	109762.0
29	152257.0
30	206345.0
31	273858.0
32	362404.0
33	486228.0
34	665213.0
35	961032.0
36	1644339.0
37	3761847.0
38	4260209.0
39	107242.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.36040578901512	20.456951774719144	14.623362407261705	23.559280029004032
2	14.506908666777893	21.021974261656844	41.81524243316598	22.655874638399283
3	17.744638706220826	26.608623654757206	30.901936043435548	24.744801595586427
4	12.257857816425508	18.558348581419004	38.93978168570436	30.244011916451125
5	13.170615670139311	38.029568978328115	35.656793626163434	13.143021725369136
6	29.468615183013775	38.15101903255726	18.96527412255377	13.4150916618752
7	26.285633188343567	33.00254788142019	23.00348844594732	17.708330484288926
8	24.586235180030734	36.533053321651494	21.147689295828663	17.733022202489106
9	25.68334520055297	16.81049402581979	20.914751425054025	36.59140934857321
10-11	24.132156237690495	26.72733857194104	28.91842057987299	20.22208461049548
12-13	25.24934844233958	24.27417722611482	28.80375654596642	21.67271778557918
14-15	22.331124169417198	25.6890844722376	26.45850822136131	25.52128313698389
16-17	21.76039718869973	28.944460196494415	27.06410228358127	22.231040331224587
18-19	21.848744156419983	27.867661963236234	28.886066182313925	21.397527698029858
20-21	22.733796699394347	26.965446148244926	28.777263821434335	21.523493330926392
22-23	22.45932118482076	27.120846286214338	28.56156326764175	21.858269261323155
24-25	22.298044719410928	27.26414574902416	28.69476471886679	21.743044812698123
26-27	22.229468021886305	27.45962610102599	28.628983119254926	21.68192275783278
28-29	22.126040281008645	27.348794099839214	28.69608200576686	21.829083613385283
30-31	22.25901588136258	27.38369561861799	28.668975719166795	21.688312780852637
32-33	22.197211419416256	27.52692998820805	28.523571776008755	21.752286816366944
34-35	22.365805947823624	27.37584386476638	28.61450909451289	21.6438410928971
36-37	22.106101029502494	27.478498296970557	28.46044995441063	21.954950719116315
38-39	22.039458427219415	27.35104414062241	28.609628717299486	21.99986871485869
40-41	22.208779036893024	27.24818555726823	28.537051811344433	22.005983594494317
42-43	22.260393788856078	27.28383697827683	28.333096194997214	22.122673037869873
44-45	22.20494443608162	27.521338770236035	28.12021973963079	22.153497054051545
46-47	22.39551818749815	27.376835456524212	27.71799320582614	22.509653150151497
48-49	22.285302309863013	27.50799266739554	27.75348394714403	22.453221075597416
50-51	22.23274199359997	27.69586353573501	27.676948856339433	22.394445614325587
52-53	22.396394607743055	27.623756890895912	27.69706633920296	22.28278216215807
54-55	22.369191842079406	27.594026600044614	27.577601387800076	22.459180170075904
56-57	22.484002432001407	27.765379510929968	27.36626742459556	22.384350632473062
58-59	22.24875290525324	27.869853096227814	27.6055871897077	22.275806808811247
60-61	22.47699248009865	28.032617117281017	27.283514501522372	22.20687590109796
62-63	22.368562369484028	28.12931528618191	27.266232798826334	22.235889545507725
64-65	22.341601167311538	27.952710702637372	27.28772884294541	22.41795928710568
66-67	22.429180546308178	27.901030333061232	27.181624966037372	22.48816415459322
68-69	22.395236105658114	27.829664048751724	27.30746207711816	22.467637768472002
70-71	22.41505285080465	27.63051960021725	27.349744716940265	22.604682832037827
72-73	22.4653073367636	27.623946992244402	27.29647316993575	22.61427250105624
74-75	22.402354491409607	27.771743220731164	27.224127185095316	22.60177510276391
76-77	22.53819975079151	27.674070093064202	27.19386543271189	22.5938647234324
78-79	22.468660337825675	27.670227034328093	27.159913761638084	22.70119886620815
80-81	22.45126178730231	27.729444730829726	27.146921507760368	22.672371974107598
82-83	22.53872133660989	27.721181402658985	27.237624859499533	22.502472401231593
84-85	22.639608954158504	27.686915893308505	27.16285772793538	22.51061742459761
86-87	22.466178212228805	27.701308271403036	27.252856689787176	22.579656826580987
88-89	22.543210330508558	27.660112878012043	27.23948385661049	22.557192934868905
90-91	22.54591969149543	27.763883752178707	27.231002203348563	22.4591943529773
92-93	22.61631908216555	27.689625570954256	27.241750709112345	22.45230463776785
94-95	22.423147722443325	27.71520537863484	27.31975112785559	22.54189577106624
96-97	22.51977031129387	27.833590886162323	27.376640084268455	22.269998718275353
98-99	23.659980389231226	29.733803318843815	27.53510179946105	19.071114492463913
100	22.579305511895047	30.718980591482925	21.370375870974208	25.33133802564782
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2668.0
1	2455.5
2	3421.5
3	4819.5
4	6382.0
5	10402.5
6	13767.0
7	15548.5
8	16329.0
9	15781.0
10	14563.5
11	13404.0
12	14850.5
13	20250.0
14	32971.5
15	51076.5
16	66930.0
17	77362.5
18	80745.5
19	77089.5
20	68989.0
21	59686.5
22	52822.0
23	52011.0
24	57743.5
25	69944.5
26	88496.5
27	109839.0
28	133772.0
29	162401.0
30	192799.0
31	224001.0
32	258951.5
33	294116.0
34	329015.5
35	362897.5
36	398108.5
37	430317.0
38	452500.5
39	471548.0
40	491898.5
41	507818.0
42	516193.5
43	525954.0
44	534886.5
45	544732.5
46	558872.5
47	569968.0
48	565922.5
49	556362.0
50	550363.0
51	533611.0
52	505284.0
53	471391.0
54	436863.5
55	402619.0
56	367905.0
57	330905.0
58	292417.0
59	252596.0
60	209542.0
61	168386.5
62	133492.5
63	102847.5
64	76212.0
65	56683.5
66	42804.0
67	32694.0
68	25183.5
69	18529.5
70	13282.0
71	9533.5
72	6597.5
73	4411.0
74	2903.0
75	1848.5
76	1187.0
77	741.5
78	453.5
79	292.0
80	204.0
81	155.5
82	123.5
83	81.5
84	50.5
85	37.0
86	28.5
87	23.5
88	18.0
89	14.0
90	11.0
91	7.5
92	5.0
93	2.5
94	0.5
95	0.0
96	0.0
97	0.0
98	0.5
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	1.5194903507804254E-4
7	0.0
8	0.0
9	0.0
10-11	0.0036277832124882656
12-13	3.038980701560851E-5
14-15	0.0023931973024791703
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	216.0
20-21	871.0
22-23	1833.0
24-25	4120.0
26-27	5521.0
28-29	9707.0
30-31	21373.0
32-33	27690.0
34-35	39576.0
36-37	56000.0
38-39	67084.0
40-41	73774.0
42-43	89981.0
44-45	108921.0
46-47	105749.0
48-49	93787.0
50-51	81819.0
52-53	79019.0
54-55	89709.0
56-57	97495.0
58-59	99942.0
60-61	102251.0
62-63	104339.0
64-65	108481.0
66-67	117053.0
68-69	105919.0
70-71	104379.0
72-73	99810.0
74-75	103106.0
76-77	101322.0
78-79	109677.0
80-81	112879.0
82-83	109656.0
84-85	110727.0
86-87	117614.0
88-89	124669.0
90-91	132343.0
92-93	139579.0
94-95	169902.0
96-97	464983.0
98-99	810450.0
100-101	8658982.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	81.41857147264298
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.59865083544376	71.32157013952676
2	8.321954142173015	13.551232362331419
3	2.1693031120395596	5.298646814202592
4	0.8401711457919233	2.7362213793164827
5	0.40008104991330634	1.6287013778608288
6	0.2188472895875326	1.0690940213326035
7	0.12736353450446264	0.7258829925942016
8	0.08151002372040549	0.5309143753613327
9	0.05505821122434798	0.40344848151529056
>10	0.18147605876744471	2.2863028845728497
>50	0.00434576324841025	0.2390257237881766
>100	0.0011851288595194091	0.15702816376335496
>500	1.7901882418585732E-5	0.010392300792462335
>1k	3.580284386110469E-5	0.041538983041648596
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	7.597451753902127E-6	3.7987258769510635E-5	0.0	7.597451753902127E-6	0.0
3	7.597451753902127E-6	3.7987258769510635E-5	0.0	7.597451753902127E-6	0.0
4	7.597451753902127E-6	7.597451753902127E-5	0.0	2.2792355261706383E-5	0.0
5	1.5194903507804254E-5	7.597451753902127E-5	0.0	3.0389807015608507E-5	0.0
6	2.2792355261706383E-5	7.597451753902127E-5	0.0	4.5584710523412766E-5	7.597451753902127E-6
7	2.2792355261706383E-5	8.35719692929234E-5	0.0	4.5584710523412766E-5	7.597451753902127E-6
8	3.0389807015608507E-5	1.0636432455462978E-4	0.0	4.5584710523412766E-5	7.597451753902127E-6
9	3.0389807015608507E-5	1.5194903507804254E-4	0.0	8.35719692929234E-5	7.597451753902127E-6
10-11	3.7987258769510635E-5	1.5194903507804254E-4	0.0	1.7094266446279787E-4	7.597451753902127E-6
12-13	3.7987258769510635E-5	1.557477609549936E-4	0.0	1.8233884209365106E-4	7.597451753902127E-6
14-15	4.558471052341276E-5	2.39319730247917E-4	0.0	1.975337456014553E-4	2.6591081138657445E-5
16-17	6.837706578511915E-5	2.507159078787702E-4	0.0	2.203261008631617E-4	4.5584710523412766E-5
18-19	7.597451753902127E-5	2.507159078787702E-4	0.0	2.583133596326723E-4	5.318216227731489E-5
20-21	8.357196929292341E-5	2.583133596326723E-4	0.0	2.8870316664828086E-4	5.698088815426595E-5
22-23	1.0636432455462978E-4	2.8870316664828086E-4	0.0	3.228916995408404E-4	8.357196929292341E-5
24-25	1.5194903507804254E-4	3.114955219099872E-4	0.0	3.798725876951064E-4	9.876687280072766E-5
26-27	1.8993629384755317E-4	3.2669042541779147E-4	0.0	5.394190745270511E-4	9.876687280072766E-5
28-29	1.8993629384755317E-4	3.3428787717169363E-4	0.0	0.0010674419714232488	9.876687280072766E-5
30-31	2.0892992323230848E-4	4.17859846464617E-4	0.0	0.0032061246401466977	9.876687280072766E-5
32-33	2.3172227849401487E-4	4.254572982185191E-4	0.0	0.0076772249973180995	1.139617763085319E-4
34-35	2.6211208550962336E-4	4.8623691224973617E-4	0.0	0.013409502345637255	1.139617763085319E-4
36-37	2.811057148943787E-4	5.736076074196106E-4	0.0	0.022150370588501653	1.139617763085319E-4
38-39	2.811057148943787E-4	6.191923179430233E-4	0.0	0.04340424187004285	1.1776050218548296E-4
40-41	3.038980701560851E-4	6.647770284664361E-4	0.0	0.06579773091466937	1.367541315702383E-4
42-43	3.114955219099872E-4	6.913681096050936E-4	0.0	0.0807457172404718	1.367541315702383E-4
44-45	3.304891512947425E-4	7.597451753902127E-4	0.0	0.1000204523401215	1.4055285744718937E-4
46-47	3.6467768418730213E-4	8.053298859136255E-4	0.0	0.11899128936961512	1.557477609549936E-4
48-49	3.6467768418730213E-4	8.129273376675276E-4	0.0	0.13695926276759365	1.7474139033974893E-4
50-51	3.6467768418730213E-4	8.737069516987446E-4	0.0	0.15273537133457143	1.7474139033974893E-4
52-53	3.7987258769510635E-4	8.737069516987446E-4	0.0	0.18948804419407295	1.8993629384755317E-4
54-55	3.7987258769510635E-4	8.737069516987446E-4	0.0	0.2321477357922334	2.0892992323230848E-4
56-57	3.7987258769510635E-4	8.813044034526468E-4	0.0	0.2753316515614131	2.1652737498621064E-4
58-59	3.950674912029106E-4	8.927005810835E-4	0.0	0.3064280215901345	2.3172227849401487E-4
60-61	4.1026239471071485E-4	9.116942104682552E-4	0.0	0.3311843181302246	2.4311845612486806E-4
62-63	4.3685347584937234E-4	9.230903880991085E-4	0.0	0.35053122902153633	2.583133596326723E-4
64-65	4.5204837935717656E-4	9.952661797611787E-4	0.0	0.3554961637427114	2.583133596326723E-4
66-67	4.634445569880298E-4	9.952661797611787E-4	0.0	0.36020278510425374	2.6211208550962336E-4
68-69	4.710420087419319E-4	0.001006662357392032	0.0	0.365000575886843	2.6591081138657447E-4
70-71	4.862369122497361E-4	0.001018058535022885	0.0	0.3693463182900749	2.735082631404766E-4
72-73	4.862369122497361E-4	0.0010256559867767871	0.0	0.3728867308073934	3.304891512947425E-4
74-75	4.862369122497361E-4	0.0010256559867767871	0.0	0.3758079510067687	3.342878771716936E-4
76-77	4.862369122497361E-4	0.0010522470679154446	0.0	0.37867978776974376	3.342878771716936E-4
78-79	4.862369122497361E-4	0.0010864356008080042	0.0	0.38059814433760397	3.4188532892559574E-4
80-81	4.862369122497361E-4	0.0010902343266849554	0.0	0.38120214175203926	3.4948278067949785E-4
82-83	4.862369122497361E-4	0.0011054292301927596	0.0	0.3816693850349042	3.8747003944900846E-4
84-85	4.862369122497361E-4	0.0011168254078236127	0.0	0.38186691878050566	3.8747003944900846E-4
86-87	4.862369122497361E-4	0.0011244228595775148	0.0	0.3819504907497986	3.950674912029106E-4
88	4.862369122497361E-4	0.0011776050218548298	0.0	0.3819694843791833	3.950674912029106E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	19185	0.0	49.0363	1
GTATCAA	34535	0.0	36.88809	1
TCAACGC	41605	0.0	30.063334	4
ATCAACG	41905	0.0	29.827415	3
CAACGCA	42595	0.0	29.355976	5
TATCAAC	43210	0.0	29.134977	2
AACGCAG	43735	0.0	28.68883	6
ACGCAGA	49920	0.0	25.142735	7
CTTATAC	15130	0.0	24.854416	1
CGCAGAG	51225	0.0	24.502388	8
TATACAC	19820	0.0	21.612488	3
GCAGAGT	58595	0.0	21.267475	9
GTACATG	46270	0.0	20.554405	1
GAGTACT	31150	0.0	20.45582	12-13
GTGGTAT	9045	0.0	19.910566	1
TACATGG	46965	0.0	19.821129	2
TTATACA	20290	0.0	19.780474	2
ACATGGG	46475	0.0	19.513441	3
CAGAGTA	57230	0.0	18.957275	10-11
AGAGTAC	53515	0.0	18.606552	10-11
>>END_MODULE