FastQCFastQC Report
Fri 27 May 2016
SRR2139516_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139516_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10136351
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA128100.051.109011
GTATCAA236050.037.914911
TCAACGC281000.031.127224
ATCAACG285700.030.6301233
CAACGCA289600.030.2198685
TATCAAC297400.029.6575982
AACGCAG298450.029.3382326
ACGCAGA343000.025.5657377
CGCAGAG354400.024.70698
GTACATG323700.021.333791
GCAGAGT414750.021.0081279
TACATGG326600.020.6018582
GTGGTAT65150.020.4300881
GAGTACT226500.019.98650712-13
ACATGGG329900.019.7932033
TGGTATC61650.019.767422
CAGAGTA403900.018.66720410-11
AGAGTAC371150.018.38716710-11
GTACTTT248350.018.21938114-15
CATGGGG233000.017.2675154
Produced by FastQC (version 0.11.5)