##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139516_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10136351
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.52683712314224	33.0	33.0	33.0	33.0	33.0
2	32.556562415804265	33.0	33.0	33.0	33.0	33.0
3	32.578895205976984	33.0	33.0	33.0	33.0	33.0
4	36.491343778446506	37.0	37.0	37.0	37.0	37.0
5	36.51609528912328	37.0	37.0	37.0	37.0	37.0
6	36.50667651504965	37.0	37.0	37.0	37.0	37.0
7	36.48402477380667	37.0	37.0	37.0	37.0	37.0
8	36.47760520526568	37.0	37.0	37.0	37.0	37.0
9	36.513712676287554	37.0	37.0	37.0	37.0	37.0
10-11	36.43925540857849	37.0	37.0	37.0	37.0	37.0
12-13	36.44120181907671	37.0	37.0	37.0	37.0	37.0
14-15	38.44328614902937	40.0	37.0	40.0	37.0	40.0
16-17	38.39902421492705	40.0	37.0	40.0	37.0	40.0
18-19	38.39580446651857	40.0	37.0	40.0	37.0	40.0
20-21	38.390724678430956	40.0	37.0	40.0	37.0	40.0
22-23	38.39421494796336	40.0	37.0	40.0	37.0	40.0
24-25	38.38164801911054	40.0	37.0	40.0	37.0	40.0
26-27	38.276219586471	40.0	37.0	40.0	37.0	40.0
28-29	38.21216971310126	40.0	37.0	40.0	37.0	40.0
30-31	38.077088656946366	40.0	37.0	40.0	33.0	40.0
32-33	38.08559978232641	40.0	37.0	40.0	33.0	40.0
34-35	38.04857157432585	40.0	37.0	40.0	33.0	40.0
36-37	37.96740208502541	40.0	37.0	40.0	33.0	40.0
38-39	37.915863012931815	40.0	37.0	40.0	33.0	40.0
40-41	37.920453329060166	40.0	37.0	40.0	33.0	40.0
42-43	37.85694043605736	40.0	37.0	40.0	33.0	40.0
44-45	37.79508645680807	40.0	37.0	40.0	33.0	40.0
46-47	37.756466807811606	40.0	37.0	40.0	33.0	40.0
48-49	37.72947740774062	40.0	37.0	40.0	33.0	40.0
50-51	37.64534156638484	40.0	37.0	40.0	33.0	40.0
52-53	37.54074574345057	40.0	37.0	40.0	33.0	40.0
54-55	37.38433929981855	40.0	37.0	40.0	33.0	40.0
56-57	37.278310502188376	40.0	37.0	40.0	33.0	40.0
58-59	37.111710865894494	37.0	37.0	40.0	33.0	40.0
60-61	36.992721532101896	37.0	37.0	40.0	33.0	40.0
62-63	36.82432719082715	37.0	37.0	40.0	33.0	40.0
64-65	36.64255762250238	37.0	37.0	40.0	33.0	40.0
66-67	36.41310629961667	37.0	37.0	40.0	33.0	40.0
68-69	36.181370684915905	37.0	37.0	40.0	33.0	40.0
70-71	35.9295007460948	37.0	37.0	37.0	33.0	40.0
72-73	35.72203194853415	37.0	33.0	37.0	33.0	40.0
74-75	35.500257856181705	37.0	33.0	37.0	33.0	40.0
76-77	33.79390807453231	35.0	33.0	37.0	27.0	37.0
78-79	34.99454196088512	37.0	33.0	37.0	33.0	37.0
80-81	35.06384334500679	37.0	33.0	37.0	33.0	37.0
82-83	34.91185170905541	37.0	33.0	37.0	33.0	37.0
84-85	34.776145455615506	37.0	33.0	37.0	33.0	37.0
86-87	34.660276112235934	37.0	33.0	37.0	33.0	37.0
88-89	34.49220459888042	37.0	33.0	37.0	33.0	37.0
90-91	34.50555352915062	37.0	33.0	37.0	33.0	37.0
92-93	34.4389661160453	37.0	33.0	37.0	33.0	37.0
94-95	34.41366188380818	37.0	33.0	37.0	33.0	37.0
96-97	34.42028863734042	37.0	33.0	37.0	33.0	37.0
98-99	34.41004958463297	37.0	33.0	37.0	33.0	37.0
100	34.43221801879223	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	2.0
18	1.0
19	5.0
20	60.0
21	372.0
22	1471.0
23	4291.0
24	10131.0
25	19576.0
26	35092.0
27	55176.0
28	81705.0
29	114232.0
30	154426.0
31	207615.0
32	275363.0
33	369078.0
34	508023.0
35	735805.0
36	1267683.0
37	2916683.0
38	3297299.0
39	82262.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.7137587283629	20.27384410820028	14.502339155382446	23.510058008054376
2	14.635375195669525	21.05197422622796	41.514781798696596	22.79786877940592
3	17.863598054171565	26.36422120741478	30.61462650612632	25.15755423228734
4	12.410215471030945	18.282752836795016	39.11495369487501	30.192077997299027
5	13.359057909498201	37.92344996735018	35.602308957138526	13.115183166013095
6	29.869702450727274	38.06055007242261	18.91360568284128	13.15614179400884
7	26.643986578602103	32.76328927441443	23.184477333115243	17.408246813868224
8	24.66071863533534	36.60699989572184	21.01294637488382	17.719335094058998
9	25.804700330523282	16.885001318521823	21.03048720392575	36.27981114702914
10-11	24.215292227416516	26.671991571443954	29.00377993587916	20.10893626526037
12-13	25.20893006173378	24.28961953895321	28.934414386879926	21.56703601243308
14-15	22.334126948696504	25.660074964711534	26.518314115312076	25.48748397127989
16-17	21.846337996780104	28.782729603582197	27.177329395953237	22.193603003684462
18-19	21.85380616752518	27.97785416073299	28.87454272252411	21.29379694921772
20-21	22.74801092253771	27.072191233990644	28.75052312988834	21.4292747135833
22-23	22.510689697955446	26.986211862105304	28.56352412362976	21.93957431630949
24-25	22.222011155256453	27.177995633972905	28.62649449391525	21.973498716855385
26-27	22.129036734933194	27.342926793781697	28.56797239423946	21.96006407704565
28-29	22.10423356914606	27.28097955104733	28.631234521086874	21.983552358719738
30-31	22.330146312015977	27.249182983886698	28.497138559597612	21.923532144499713
32-33	22.13228755514753	27.397150371248387	28.422750050767974	22.047812022836112
34-35	22.302822099471957	27.402059320691883	28.37593997118818	21.91917860864798
36-37	22.316664554142278	27.32270564430037	28.307855028958826	22.05277477259852
38-39	22.156405431475402	27.449681225763495	28.389459544919372	22.004453797841737
40-41	22.353591823573325	27.35881011018435	28.257042076777683	22.03055598946464
42-43	22.25045792098071	27.452376672217802	28.04838302189391	22.248782384907578
44-45	22.34865762067873	27.499307082845455	27.902863049846594	22.24917224662922
46-47	22.407780263109448	27.550844674497654	27.640846125274955	22.400528937117944
48-49	22.34295751288076	27.624658390736656	27.6313297261342	22.40105437024838
50-51	22.366447075147132	27.749479711150215	27.497503351114176	22.386569862588477
52-53	22.51984582049471	27.61993408219069	27.410219820419268	22.45000027689534
54-55	22.532193893042006	27.55262934703341	27.448489403295675	22.466687356628913
56-57	22.479662693953355	27.641697466344223	27.399215117216812	22.479424722485607
58-59	22.447503587723403	27.61837783359864	27.432964202848115	22.50115437582984
60-61	22.51954628539467	27.633303400660843	27.43784456872149	22.409305745222994
62-63	22.46685495592182	27.752185762601727	27.400844622911663	22.380114658564786
64-65	22.479529237050144	27.668447757905774	27.337298063284273	22.514724941759813
66-67	22.523123450440977	27.70588506205912	27.201368930197063	22.569622557302843
68-69	22.45054293595025	27.7222386881456	27.269747373666792	22.557471002237357
70-71	22.460590180534766	27.536434967420213	27.330949920164116	22.67202493188091
72-73	22.52109313993866	27.586174316717237	27.20854630339647	22.68418623994763
74-75	22.475365442146007	27.70799223036679	27.173311026357204	22.643331301129997
76-77	22.590671198002717	27.575930283370965	27.163955799394508	22.66944271923181
78-79	22.52889565647045	27.57901197323644	27.066631814250087	22.825460556043026
80-81	22.518267259572518	27.687686994750717	27.083645804599087	22.71039994107768
82-83	22.63922290911511	27.607988507853953	27.157462065956032	22.595326517074902
84-85	22.640513524946645	27.62925461882513	27.11700112648475	22.613230729743474
86-87	22.51508381638333	27.651631969943207	27.22488204530303	22.608402168370432
88-89	22.58411457355437	27.630700831277167	27.17098051686368	22.61420407830478
90-91	22.605367794460832	27.676329089527485	27.162487309706982	22.555815806304697
92-93	22.650849316022136	27.621380586067833	27.17439570767219	22.55337439023784
94-95	22.490237238076777	27.635592795206488	27.26637750421681	22.60779246249993
96-97	22.591461327830135	27.74850259761836	27.300369646860794	22.359666427690705
98-99	23.7192879256663	29.578984170021748	27.535517536047337	19.166210368264615
100	22.578611164787333	30.603024623687375	21.359710877982955	25.45865333354234
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2256.0
1	1984.5
2	2448.0
3	3334.0
4	4379.0
5	7006.5
6	9261.5
7	10624.5
8	11409.0
9	11297.0
10	10591.5
11	9856.0
12	10972.0
13	14655.0
14	23118.5
15	35318.0
16	45917.0
17	52758.0
18	54907.5
19	52302.5
20	46942.0
21	41376.5
22	37748.5
23	37985.0
24	43109.0
25	53244.5
26	67164.0
27	83406.0
28	102555.5
29	125136.5
30	148861.0
31	173087.5
32	199465.0
33	226005.0
34	252218.0
35	277105.5
36	302887.5
37	326104.0
38	344367.5
39	361380.5
40	376483.0
41	387412.0
42	393102.0
43	399979.5
44	408108.5
45	415073.0
46	417764.5
47	416436.5
48	414220.5
49	409235.0
50	402930.0
51	392165.0
52	377005.0
53	358379.5
54	336497.5
55	314091.5
56	289852.5
57	261676.5
58	232176.0
59	202114.0
60	169214.0
61	137670.0
62	109789.5
63	85024.5
64	63563.0
65	46862.0
66	35138.5
67	27218.5
68	21068.0
69	15701.5
70	11447.5
71	8138.0
72	5540.5
73	3749.5
74	2535.0
75	1647.0
76	1074.0
77	682.0
78	447.5
79	319.5
80	225.0
81	160.0
82	123.5
83	87.0
84	57.5
85	41.0
86	33.0
87	29.0
88	20.5
89	10.0
90	3.5
91	2.0
92	1.5
93	0.5
94	0.5
95	1.0
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	1.28251280958996E-4
7	0.0
8	0.0
9	0.0
10-11	0.0032802731476050894
12-13	4.4394674178114E-5
14-15	0.002451572562946962
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	142.0
20-21	584.0
22-23	1285.0
24-25	2558.0
26-27	3747.0
28-29	6594.0
30-31	14721.0
32-33	19119.0
34-35	27496.0
36-37	39589.0
38-39	46682.0
40-41	51926.0
42-43	63002.0
44-45	76125.0
46-47	74753.0
48-49	66791.0
50-51	57591.0
52-53	54284.0
54-55	58691.0
56-57	63987.0
58-59	65909.0
60-61	66421.0
62-63	65903.0
64-65	70052.0
66-67	75597.0
68-69	76577.0
70-71	76948.0
72-73	74185.0
74-75	76313.0
76-77	75367.0
78-79	81629.0
80-81	83784.0
82-83	81776.0
84-85	83062.0
86-87	87964.0
88-89	94293.0
90-91	100877.0
92-93	106509.0
94-95	129764.0
96-97	369212.0
98-99	638635.0
100-101	6825907.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.74352572301189
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.31962174463887	75.69259662886498
2	7.531567368775788	12.765031461008961
3	1.8036901614809377	4.585531907874101
4	0.6285387422398059	2.130583562836341
5	0.28437904737688413	1.20496415582343
6	0.14409474136336348	0.7326657852766164
7	0.08354457467597175	0.49559032691499094
8	0.052133690509256994	0.3534394194165409
9	0.036152343431994846	0.27573093410187743
>10	0.11265962558305552	1.4918218555481695
>50	0.0029090623476473627	0.16295865261979353
>100	6.701280427618475E-4	0.09047328588584878
>500	3.876953366083947E-5	0.018612023828388266
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	9.865483150691999E-6	0.0
4	0.0	2.9596449452076E-5	0.0	2.9596449452076E-5	0.0
5	0.0	2.9596449452076E-5	0.0	4.932741575346E-5	0.0
6	9.865483150691999E-6	2.9596449452076E-5	0.0	5.9192898904152E-5	9.865483150691999E-6
7	9.865483150691999E-6	2.9596449452076E-5	0.0	5.9192898904152E-5	1.9730966301383997E-5
8	9.865483150691999E-6	2.9596449452076E-5	0.0	5.9192898904152E-5	2.9596449452076E-5
9	9.865483150691999E-6	5.9192898904152E-5	0.0	6.9058382054844E-5	3.9461932602767995E-5
10-11	1.4798224726037998E-5	5.9192898904152E-5	0.0	8.878934835622798E-5	3.9461932602767995E-5
12-13	1.9730966301383997E-5	5.9192898904152E-5	0.0	1.18385797808304E-4	4.439467417811399E-5
14-15	1.9730966301383997E-5	1.4304950568503399E-4	0.0	1.4798224726037998E-4	4.932741575346E-5
16-17	5.9192898904152E-5	1.4798224726037998E-4	0.0	1.5784773041107198E-4	4.932741575346E-5
18-19	5.9192898904152E-5	1.5784773041107198E-4	0.0	1.87444179863148E-4	5.4260157328806E-5
20-21	5.9192898904152E-5	1.67713213561764E-4	0.0	2.31838854041262E-4	6.9058382054844E-5
22-23	5.9192898904152E-5	1.9730966301384E-4	0.0	2.51569820342646E-4	7.892386520553599E-5
24-25	5.9192898904152E-5	2.17040629315224E-4	0.0	3.25560943972836E-4	8.8789348356228E-5
26-27	5.9192898904152E-5	2.36771595616608E-4	0.0	4.5874496650717797E-4	8.8789348356228E-5
28-29	6.9058382054844E-5	2.41704337191954E-4	0.0	9.174899330143559E-4	9.865483150692E-5
30-31	6.9058382054844E-5	2.66368045068684E-4	0.0	0.002619285776508726	1.18385797808304E-4
32-33	7.399112363018999E-5	2.66368045068684E-4	0.0	0.006210321643360614	1.38116764109688E-4
34-35	7.892386520553599E-5	3.1569546082214396E-4	0.0	0.011167726926583344	1.5291498883572598E-4
36-37	7.892386520553599E-5	3.6995561815094996E-4	0.0	0.018887467491999835	1.5784773041107198E-4
38-39	8.385660678088199E-5	3.84753842876988E-4	0.0	0.038120226894273884	1.5784773041107198E-4
40-41	8.8789348356228E-5	4.3408125863044796E-4	0.0	0.05921756261202873	1.62780471986418E-4
42-43	8.8789348356228E-5	4.4394674178113995E-4	0.0	0.07316735578710722	1.7264595513711E-4
44-45	1.03587573082266E-4	4.88341415959254E-4	0.0	0.09093015819992815	1.87444179863148E-4
46-47	1.28251280958996E-4	5.22870606986676E-4	0.0	0.1082095519383652	1.87444179863148E-4
48-49	1.28251280958996E-4	5.22870606986676E-4	0.0	0.12474410169892498	1.92376921438494E-4
50-51	1.4798224726037998E-4	6.01794472192212E-4	0.0	0.1407015206951693	2.0717514616453198E-4
52-53	1.5784773041107198E-4	6.01794472192212E-4	0.0	0.18071592035437606	2.2197337089056998E-4
54-55	1.5784773041107198E-4	6.06727213767558E-4	0.0	0.22931822309626018	2.466370787673E-4
56-57	1.5784773041107198E-4	6.116599553429039E-4	0.0	0.2763025866014308	2.56502561917992E-4
58-59	1.5784773041107198E-4	6.116599553429039E-4	0.0	0.30734432933508316	2.66368045068684E-4
60-61	1.6771321356176397E-4	6.165926969182499E-4	0.0	0.3290335940418796	2.66368045068684E-4
62-63	1.9730966301384E-4	6.313909216442879E-4	0.0	0.34285513593599903	2.76233528219376E-4
64-65	1.9730966301384E-4	7.49776719452592E-4	0.0	0.3475856351067559	2.76233528219376E-4
66-67	2.1704062931522398E-4	7.49776719452592E-4	0.0	0.3521336228392249	2.8116626979472195E-4
68-69	2.1704062931522398E-4	7.744404273293219E-4	0.0	0.35672107250429663	2.9596449452075997E-4
70-71	2.2197337089056998E-4	7.941713936307059E-4	0.0	0.3606278038319707	3.0582997767145196E-4
72-73	2.76233528219376E-4	7.99104135206052E-4	0.0	0.36383901859752094	3.0582997767145196E-4
74-75	2.76233528219376E-4	8.188351015074359E-4	0.0	0.3666210848460161	3.0582997767145196E-4
76-77	2.76233528219376E-4	8.632297756855499E-4	0.0	0.36921077417307274	3.0582997767145196E-4
78-79	2.76233528219376E-4	8.78028000411588E-4	0.0	0.37087804082553966	3.10762719246798E-4
80-81	2.76233528219376E-4	8.78028000411588E-4	0.0	0.37147490255615656	3.25560943972836E-4
82-83	2.76233528219376E-4	8.78028000411588E-4	0.0	0.37191391655636236	3.35426427123528E-4
84-85	2.76233528219376E-4	8.78028000411588E-4	0.0	0.3721605536351297	3.35426427123528E-4
86-87	2.8116626979472195E-4	8.878934835622799E-4	0.0	0.3722592084666365	3.35426427123528E-4
88	3.0582997767145196E-4	9.66817348767816E-4	0.0	0.3722641412082119	3.35426427123528E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	12810	0.0	51.10901	1
GTATCAA	23605	0.0	37.91491	1
TCAACGC	28100	0.0	31.12722	4
ATCAACG	28570	0.0	30.630123	3
CAACGCA	28960	0.0	30.219868	5
TATCAAC	29740	0.0	29.657598	2
AACGCAG	29845	0.0	29.338232	6
ACGCAGA	34300	0.0	25.565737	7
CGCAGAG	35440	0.0	24.7069	8
GTACATG	32370	0.0	21.33379	1
GCAGAGT	41475	0.0	21.008127	9
TACATGG	32660	0.0	20.601858	2
GTGGTAT	6515	0.0	20.430088	1
GAGTACT	22650	0.0	19.986507	12-13
ACATGGG	32990	0.0	19.793203	3
TGGTATC	6165	0.0	19.76742	2
CAGAGTA	40390	0.0	18.667204	10-11
AGAGTAC	37115	0.0	18.387167	10-11
GTACTTT	24835	0.0	18.219381	14-15
CATGGGG	23300	0.0	17.267515	4
>>END_MODULE