FastQCFastQC Report
Fri 27 May 2016
SRR2139515_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139515_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9416376
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA118450.048.663761
GTATCAA211850.036.7270281
TCAACGC251850.030.156834
ATCAACG256100.029.7067953
CAACGCA257750.029.5015965
TATCAAC265750.028.9205272
AACGCAG264600.028.8194526
ACGCAGA307200.024.8509837
CGCAGAG315100.024.2009148
GTACATG302050.020.7847631
GCAGAGT371100.020.3751499
TACATGG303250.020.2611262
GAGTACT191450.019.62203812-13
ACATGGG305250.019.5342833
GTGGTAT59250.018.7285541
AGAGTAC331700.018.48618510-11
CAGAGTA357800.018.41095510-11
CTTATAC115300.018.2746891
CATGGGG216050.017.6668784
GTACTTT218050.017.29769314-15
Produced by FastQC (version 0.11.5)