FastQCFastQC Report
Fri 27 May 2016
SRR2139514_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139514_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6164015
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA72550.047.498311
GTATCAA127800.036.766041
TCAACGC154150.029.750734
ATCAACG155950.029.4624523
CAACGCA158150.029.082615
TATCAAC163800.028.4474282
AACGCAG161850.028.3909766
ACGCAGA185200.024.8114457
CGCAGAG190500.024.0760338
GTACATG187250.022.8940641
TACATGG188150.022.5541732
ACATGGG191400.021.4012093
GCAGAGT224000.020.4762069
GAGTACT117550.019.5445812-13
CATGGGG139350.018.7571054
AGAGTAC200650.018.57013510-11
CTTATAC239050.018.2776431
CAGAGTA218400.018.24194710-11
TATACAC263500.017.7660773
GTGGTAT40600.017.615961
Produced by FastQC (version 0.11.5)