##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139514_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6164015
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.521575466639845	33.0	33.0	33.0	33.0	33.0
2	32.560136372153536	33.0	33.0	33.0	33.0	33.0
3	32.58174679977255	33.0	33.0	33.0	33.0	33.0
4	36.48939952287592	37.0	37.0	37.0	37.0	37.0
5	36.512382108090264	37.0	37.0	37.0	37.0	37.0
6	36.497652098510464	37.0	37.0	37.0	37.0	37.0
7	36.47639874335153	37.0	37.0	37.0	37.0	37.0
8	36.468808073958286	37.0	37.0	37.0	37.0	37.0
9	36.51087578469553	37.0	37.0	37.0	37.0	37.0
10-11	36.43041962097756	37.0	37.0	37.0	37.0	37.0
12-13	36.431131089071	37.0	37.0	37.0	37.0	37.0
14-15	38.42560149512939	40.0	37.0	40.0	37.0	40.0
16-17	38.36667894221542	40.0	37.0	40.0	37.0	40.0
18-19	38.363631172214866	40.0	37.0	40.0	37.0	40.0
20-21	38.36193406830037	40.0	37.0	40.0	37.0	40.0
22-23	38.35807009950924	40.0	37.0	40.0	37.0	40.0
24-25	38.343437627102915	40.0	37.0	40.0	37.0	40.0
26-27	38.231751457809054	40.0	37.0	40.0	37.0	40.0
28-29	38.16754893487388	40.0	37.0	40.0	37.0	40.0
30-31	38.037936786128135	40.0	37.0	40.0	33.0	40.0
32-33	38.029845000180295	40.0	37.0	40.0	33.0	40.0
34-35	37.979863139426655	40.0	37.0	40.0	33.0	40.0
36-37	37.88400204450643	40.0	37.0	40.0	33.0	40.0
38-39	37.81898954974672	40.0	37.0	40.0	33.0	40.0
40-41	37.83798739825784	40.0	37.0	40.0	33.0	40.0
42-43	37.77140263375214	40.0	37.0	40.0	33.0	40.0
44-45	37.70749076231383	40.0	37.0	40.0	33.0	40.0
46-47	37.66593689122311	40.0	37.0	40.0	33.0	40.0
48-49	37.6383798405718	40.0	37.0	40.0	33.0	40.0
50-51	37.51805625618202	40.0	37.0	40.0	33.0	40.0
52-53	37.43187357538312	40.0	37.0	40.0	33.0	40.0
54-55	37.286627257526675	40.0	37.0	40.0	33.0	40.0
56-57	37.15605720621279	38.5	37.0	40.0	33.0	40.0
58-59	37.00268055502943	37.0	37.0	40.0	33.0	40.0
60-61	36.88086549966839	37.0	37.0	40.0	33.0	40.0
62-63	36.73293013083387	37.0	37.0	40.0	33.0	40.0
64-65	36.57623013572359	37.0	37.0	40.0	33.0	40.0
66-67	36.35374132409503	37.0	37.0	40.0	33.0	40.0
68-69	36.12268652928499	37.0	37.0	40.0	33.0	40.0
70-71	35.87666053850697	37.0	35.0	37.0	33.0	40.0
72-73	35.674944398855466	37.0	33.0	37.0	33.0	40.0
74-75	35.44811763588472	37.0	33.0	37.0	33.0	40.0
76-77	33.73339069400923	35.0	33.0	37.0	27.0	37.0
78-79	34.925493822603	37.0	33.0	37.0	33.0	37.0
80-81	34.990468776642516	37.0	33.0	37.0	33.0	37.0
82-83	34.83954418069432	37.0	33.0	37.0	33.0	37.0
84-85	34.71708989097917	37.0	33.0	37.0	33.0	37.0
86-87	34.598122986739156	37.0	33.0	37.0	33.0	37.0
88-89	34.43078043340846	37.0	33.0	37.0	30.0	37.0
90-91	34.4499193731975	37.0	33.0	37.0	33.0	37.0
92-93	34.39582857797371	37.0	33.0	37.0	33.0	37.0
94-95	34.351442028106796	37.0	33.0	37.0	27.0	37.0
96-97	34.35804782202612	37.0	33.0	37.0	30.0	37.0
98-99	34.37322811906294	37.0	33.0	37.0	33.0	37.0
100	34.37914110458961	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	4.0
19	7.0
20	72.0
21	278.0
22	1159.0
23	3232.0
24	6945.0
25	13701.0
26	23391.0
27	36489.0
28	53834.0
29	75122.0
30	100517.0
31	132919.0
32	174856.0
33	231901.0
34	317298.0
35	457332.0
36	786160.0
37	1780307.0
38	1925417.0
39	43074.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.850435957732095	20.42188411287124	14.398975343181352	24.328704586215316
2	14.38138940284863	20.79198379627564	41.83083590808913	22.9957908927866
3	17.5996975996976	26.384085697390418	31.03118665350425	24.98503004940773
4	12.256702814642729	18.719519663725674	38.19893364957743	30.824843872054174
5	13.27655756840306	37.794051442120114	35.757992152841936	13.171398836634888
6	29.686912668220742	37.864233916975	18.758406677223665	13.690446737580587
7	26.31166536745936	32.62756498808001	22.62155429537404	18.439215349086595
8	24.563145936536497	36.47972952693983	21.24740124740125	17.70972328912243
9	25.380458253339466	16.709111952049426	20.520037754618986	37.39039203999212
10-11	24.12875818133368	26.412802811251655	28.962008435539587	20.49643057187507
12-13	25.19910529058791	23.808785267713198	28.669774761164096	22.322334680534798
14-15	22.319185225239433	25.367054718769705	25.84343616981753	26.47032388617333
16-17	22.19713936452134	28.814855252623495	26.430621924184155	22.557383458671012
18-19	22.383024700621267	27.610623919636794	27.984536053205584	22.021815326536355
20-21	22.91740298002595	26.731539304227987	27.923598354768046	22.427459360978023
22-23	22.553393471194603	27.228845016236185	27.730743807713736	22.487017704855475
24-25	22.644820953839126	27.257070504839298	27.753345642034255	22.34476289928732
26-27	22.597288484785324	27.399286450199313	27.894403135346717	22.109021929668643
28-29	22.456940339306577	26.92928578220229	28.144799951345977	22.468973927145154
30-31	22.333014788675502	26.664187230786013	28.318755539921813	22.68404244061667
32-33	22.024896418269726	27.273558523209424	28.136450558664723	22.565094499856126
34-35	22.50094715348751	27.05061382564965	28.13959140753456	22.308847613328282
36-37	22.312474833721378	27.166977813384197	28.247855405178214	22.272691947716215
38-39	22.27493368072315	26.92126155901922	28.165528903630886	22.638275856626734
40-41	22.477521190944497	26.77813849910118	28.306643853852137	22.437696456102188
42-43	22.612617152447427	26.718565893734958	28.216275872786927	22.452541081030684
44-45	22.230527549683067	27.168112512375114	28.01172633492864	22.589633603013183
46-47	22.81260152517016	26.702191318430597	27.541575530852956	22.94363162554629
48-49	22.53450572196914	26.91788189584235	27.56487584726711	22.982736534921404
50-51	22.28586899336563	27.154513900750892	27.698151463182995	22.86146564270048
52-53	22.379007495830177	27.08854686003016	27.956119955894188	22.576325688245475
54-55	22.438819413517415	26.992186692032146	27.699492882094205	22.86950101235623
56-57	22.67671542459549	27.045206497636652	27.345400313804163	22.932677763963692
58-59	22.066167449900508	27.17257692238278	27.945848535375312	22.8154070923414
60-61	22.57163929259641	27.624401501291363	27.481125526495266	22.32283367961696
62-63	21.978816601749195	27.980873136605673	27.48985040492093	22.550459856724206
64-65	21.95904616324089	27.948578729492446	27.31774295061381	22.77463215665285
66-67	22.084709356683646	28.0937905051944	27.02428963640326	22.797210501718695
68-69	22.15247941657054	27.959823502616416	26.966316376319256	22.921380704493792
70-71	22.21699279289558	27.49925581198044	26.951747360783813	23.332004034340162
72-73	22.36874074139618	27.158291616617667	26.915060858844498	23.557906783141654
74-75	22.44746404334173	27.22713383259392	27.02753181009142	23.297870313972933
76-77	22.709887525208842	27.091360503926644	26.652019466758343	23.54673250410617
78-79	22.81435755275327	27.128859873194088	26.28315954911102	23.77362302494162
80-81	22.725480794538296	27.441259109901672	26.394559730362932	23.438700365197104
82-83	22.722094652339393	27.242624953721556	26.46899763058222	23.56628276335683
84-85	22.782344607161022	27.519647147090254	26.4222089955495	23.275799250199224
86-87	23.057084732940197	27.445370943151094	26.520261951862743	22.977282372045966
88-89	22.910422936828486	27.509141831506422	26.759359003281663	22.82107622838343
90-91	22.874288759862235	27.31030814667232	26.632064499204645	23.183338594260803
92-93	22.871022503990396	26.94969256380751	26.87433394067358	23.30495099152852
94-95	22.300172433688726	27.271828761807637	27.308740637558294	23.119258166945347
96-97	22.457580563590785	27.197702544369417	27.416226278370132	22.928490613669663
98-99	23.700437978649486	29.016994980965556	27.780505500071957	19.502061540313004
100	22.62189099959928	30.18605295553173	21.46358194753322	25.728474097335774
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1036.0
1	942.5
2	1268.0
3	1773.0
4	2344.0
5	3779.5
6	5000.5
7	5679.0
8	5994.0
9	5832.5
10	5452.0
11	5093.0
12	5582.5
13	7372.0
14	11770.0
15	18162.0
16	23795.0
17	27486.0
18	28616.0
19	27343.0
20	24490.5
21	21426.0
22	19443.5
23	19482.0
24	22158.5
25	27331.5
26	34710.0
27	43817.5
28	54115.5
29	66527.0
30	79890.0
31	93667.5
32	108819.0
33	124413.5
34	140160.0
35	154870.0
36	170332.0
37	184458.5
38	195294.0
39	205924.5
40	216852.5
41	225490.0
42	229992.5
43	235197.5
44	241595.0
45	247424.5
46	251958.0
47	256135.0
48	274553.0
49	283285.5
50	272660.5
51	265975.0
52	258358.5
53	243352.5
54	223601.5
55	204740.0
56	186634.5
57	168592.0
58	148912.0
59	127351.5
60	105293.5
61	85447.5
62	67850.5
63	52165.5
64	38793.0
65	28480.0
66	21514.5
67	16503.0
68	12746.5
69	9413.0
70	6664.0
71	4841.5
72	3430.0
73	2354.0
74	1540.5
75	970.5
76	641.0
77	409.5
78	270.5
79	187.5
80	125.5
81	78.0
82	55.0
83	34.0
84	22.0
85	19.5
86	19.5
87	19.0
88	16.0
89	10.5
90	6.5
91	4.0
92	3.0
93	3.0
94	3.0
95	2.5
96	2.0
97	2.0
98	1.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	2.595710750217188E-4
7	0.0
8	0.0
9	1.6223192188857426E-5
10-11	0.0034474283401322026
12-13	4.055798047214356E-5
14-15	0.0024659252127063286
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	86.0
20-21	366.0
22-23	768.0
24-25	1800.0
26-27	2325.0
28-29	4345.0
30-31	10438.0
32-33	13513.0
34-35	18760.0
36-37	26673.0
38-39	20189.0
40-41	33003.0
42-43	38255.0
44-45	44787.0
46-47	44190.0
48-49	40794.0
50-51	23429.0
52-53	10863.0
54-55	16032.0
56-57	18837.0
58-59	34680.0
60-61	54977.0
62-63	56243.0
64-65	57167.0
66-67	64106.0
68-69	46849.0
70-71	45005.0
72-73	43574.0
74-75	45102.0
76-77	43834.0
78-79	47435.0
80-81	49509.0
82-83	47813.0
84-85	48687.0
86-87	51611.0
88-89	55286.0
90-91	59476.0
92-93	63759.0
94-95	77653.0
96-97	228525.0
98-99	415745.0
100-101	4157526.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.621597581556
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.21249414837038	78.14350364948614
2	7.167879137113417	12.417862842565384
3	1.5810940264216982	4.108706714859072
4	0.5143275954713236	1.7820751200002525
5	0.21857131776257324	0.9466498365050011
6	0.10991900051331958	0.5712815657418969
7	0.05735190207145242	0.3477539367239111
8	0.036297425956080445	0.251531281953114
9	0.024382395295687767	0.19008378300397408
>10	0.07535434635754046	1.0094485146773433
>50	0.0015870543867050827	0.08960548346639151
>100	7.416502798602237E-4	0.1414972710174917
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	3.244638437771485E-5	0.0	0.0	0.0
3	0.0	3.244638437771485E-5	0.0	0.0	0.0
4	0.0	3.244638437771485E-5	0.0	0.0	1.6223192188857426E-5
5	0.0	3.244638437771485E-5	0.0	0.0	1.6223192188857426E-5
6	1.1356234532200197E-4	3.244638437771485E-5	0.0	1.6223192188857426E-5	1.6223192188857426E-5
7	1.1356234532200197E-4	3.244638437771485E-5	0.0	1.6223192188857426E-5	1.6223192188857426E-5
8	1.1356234532200197E-4	3.244638437771485E-5	0.0	1.6223192188857426E-5	4.8669576566572275E-5
9	1.1356234532200197E-4	8.111596094428713E-5	0.0	1.6223192188857426E-5	4.8669576566572275E-5
10-11	1.1356234532200197E-4	8.111596094428713E-5	0.0	5.6781172661000986E-5	4.8669576566572275E-5
12-13	1.1356234532200197E-4	8.111596094428713E-5	0.0	6.48927687554297E-5	4.8669576566572275E-5
14-15	1.1356234532200197E-4	1.297855375108594E-4	0.0	6.48927687554297E-5	7.300436484985841E-5
16-17	1.4600872969971682E-4	1.297855375108594E-4	0.0	7.300436484985841E-5	8.111596094428713E-5
18-19	1.4600872969971682E-4	1.6223192188857425E-4	0.0	8.111596094428713E-5	8.111596094428713E-5
20-21	1.4600872969971682E-4	1.946783062662891E-4	0.0	1.0545074922757325E-4	8.111596094428713E-5
22-23	1.6223192188857425E-4	2.2712469064400394E-4	0.0	1.5412032579414554E-4	8.111596094428713E-5
24-25	1.8656671017186038E-4	2.2712469064400394E-4	0.0	2.7579426721057625E-4	8.922755703871584E-5
26-27	1.946783062662891E-4	2.2712469064400394E-4	0.0	5.840349187988673E-4	9.733915313314455E-5
28-29	1.946783062662891E-4	2.2712469064400394E-4	0.0	0.0016385424110746	1.1356234532200197E-4
30-31	1.946783062662891E-4	2.4334788283286138E-4	0.0	0.005840349187988673	1.297855375108594E-4
32-33	1.946783062662891E-4	2.4334788283286138E-4	0.0	0.014795551276237972	1.4600872969971682E-4
34-35	2.1090149845514654E-4	3.001290554938623E-4	0.0	0.02612745102015488	1.4600872969971682E-4
36-37	2.2712469064400394E-4	3.6502182424929207E-4	0.0	0.04548983089755622	1.4600872969971682E-4
38-39	2.2712469064400394E-4	3.893566125325782E-4	0.0	0.16582535895840617	1.6223192188857425E-4
40-41	2.3523628673843266E-4	4.2180299691029307E-4	0.0	0.2937371177714525	1.6223192188857425E-4
42-43	2.5145947892729007E-4	4.380261890991505E-4	0.0	0.3307827771347085	1.6223192188857425E-4
44-45	2.595710750217188E-4	4.704725734768653E-4	0.0	0.37997149585132417	1.6223192188857425E-4
46-47	2.595710750217188E-4	4.8669576566572276E-4	0.0	0.4289330898772959	1.7034351798300294E-4
48-49	2.676826711161475E-4	4.8669576566572276E-4	0.0	0.4743093584295301	1.7845511407743166E-4
50-51	2.9201745939943363E-4	5.840349187988673E-4	0.0	0.51676545238777	1.946783062662891E-4
52-53	2.9201745939943363E-4	5.840349187988673E-4	0.0	0.8834501538364199	2.109014984551465E-4
54-55	2.9201745939943363E-4	6.002581109877247E-4	0.0	1.3777627082348114	2.2712469064400394E-4
56-57	2.9201745939943363E-4	6.164813031765821E-4	0.0	1.9205177145091308	2.5145947892729007E-4
58-59	2.9201745939943363E-4	6.164813031765821E-4	0.0	2.4813940264584042	2.595710750217188E-4
60-61	3.163522476827198E-4	6.327044953654396E-4	0.0	2.639399806781781	2.595710750217188E-4
62-63	4.0557980472143563E-4	6.570392836487257E-4	0.0	2.645832302484663	2.595710750217188E-4
64-65	4.136914008158643E-4	7.462668406874415E-4	0.0	2.650926384831964	2.595710750217188E-4
66-67	4.2991459300472176E-4	7.543784367818702E-4	0.0	2.6547956161690065	2.595710750217188E-4
68-69	4.3802618909915045E-4	7.706016289707277E-4	0.0	2.658891972196693	2.676826711161475E-4
70-71	4.461377851935792E-4	7.787132250651564E-4	0.0	2.662866654282963	2.757942672105762E-4
72-73	5.029189578545801E-4	7.787132250651564E-4	0.0	2.66575438249258	2.9201745939943363E-4
74-75	5.029189578545801E-4	8.030480133484426E-4	0.0	2.668041852591209	3.0824065158829107E-4
76-77	5.029189578545801E-4	8.192712055373E-4	0.0	2.6707754604750313	3.0824065158829107E-4
78-79	5.029189578545801E-4	8.273828016317286E-4	0.0	2.6721706550032733	3.0824065158829107E-4
80-81	5.029189578545801E-4	8.273828016317286E-4	0.0	2.67264112757675	3.163522476827198E-4
82-83	5.029189578545801E-4	8.354943977261573E-4	0.0	2.6731197117463212	3.244638437771485E-4
84-85	5.029189578545801E-4	8.598291860094435E-4	0.0	2.6733468364369655	3.244638437771485E-4
86-87	5.110305539490088E-4	9.084987625760158E-4	0.0	2.6734198408018153	3.4879863206043463E-4
88	5.191421500434376E-4	9.733915313314455E-4	0.0	2.6734198408018153	3.7313342034372076E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7255	0.0	47.49831	1
GTATCAA	12780	0.0	36.76604	1
TCAACGC	15415	0.0	29.75073	4
ATCAACG	15595	0.0	29.462452	3
CAACGCA	15815	0.0	29.08261	5
TATCAAC	16380	0.0	28.447428	2
AACGCAG	16185	0.0	28.390976	6
ACGCAGA	18520	0.0	24.811445	7
CGCAGAG	19050	0.0	24.076033	8
GTACATG	18725	0.0	22.894064	1
TACATGG	18815	0.0	22.554173	2
ACATGGG	19140	0.0	21.401209	3
GCAGAGT	22400	0.0	20.476206	9
GAGTACT	11755	0.0	19.54458	12-13
CATGGGG	13935	0.0	18.757105	4
AGAGTAC	20065	0.0	18.570135	10-11
CTTATAC	23905	0.0	18.277643	1
CAGAGTA	21840	0.0	18.241947	10-11
TATACAC	26350	0.0	17.766077	3
GTGGTAT	4060	0.0	17.61596	1
>>END_MODULE