FastQCFastQC Report
Fri 27 May 2016
SRR2139513_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139513_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5488636
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA66850.048.3502731
CTTATAC99750.047.276051
TATACAC122750.040.2289853
TTATACA122700.039.443952
GTATCAA119450.035.93571
TCAACGC139700.030.084014
ATCAACG140600.029.921843
CAACGCA142300.029.4466135
TACACAT173950.028.857445
AACGCAG146800.028.5730846
TATCAAC149550.028.5313742
ACACATC180700.026.6910156
ACATCTC190500.025.3628238
ACGCAGA167250.025.104967
ATACACA204650.024.9442884
CACATCT194350.024.8163977
CGCAGAG173150.024.2495218
CATCTCC219850.021.8214939
GCAGAGT203400.020.4120399
TGGTATC32450.020.1583632
Produced by FastQC (version 0.11.5)