##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139513_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5488636
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.497145738941335	33.0	33.0	33.0	33.0	33.0
2	32.539693832857566	33.0	33.0	33.0	33.0	33.0
3	32.55905365194558	33.0	33.0	33.0	33.0	33.0
4	36.459638059437715	37.0	37.0	37.0	37.0	37.0
5	36.480485497671914	37.0	37.0	37.0	37.0	37.0
6	36.46726891708614	37.0	37.0	37.0	37.0	37.0
7	36.44332417015812	37.0	37.0	37.0	37.0	37.0
8	36.43401493558691	37.0	37.0	37.0	37.0	37.0
9	36.48177689320261	37.0	37.0	37.0	37.0	37.0
10-11	36.39724979029398	37.0	37.0	37.0	37.0	37.0
12-13	36.39924564135789	37.0	37.0	37.0	37.0	37.0
14-15	38.35662867787188	40.0	37.0	40.0	37.0	40.0
16-17	38.2919426611639	40.0	37.0	40.0	37.0	40.0
18-19	38.286442660799516	40.0	37.0	40.0	37.0	40.0
20-21	38.289905066138	40.0	37.0	40.0	37.0	40.0
22-23	38.282376924269876	40.0	37.0	40.0	37.0	40.0
24-25	38.2657432650953	40.0	37.0	40.0	37.0	40.0
26-27	38.134589593678456	40.0	37.0	40.0	35.0	40.0
28-29	38.03995422104527	40.0	37.0	40.0	33.0	40.0
30-31	37.91736897793674	40.0	37.0	40.0	33.0	40.0
32-33	37.90907705906912	40.0	37.0	40.0	33.0	40.0
34-35	37.84231491953064	40.0	37.0	40.0	33.0	40.0
36-37	37.75985047011578	40.0	37.0	40.0	33.0	40.0
38-39	37.6692539638146	40.0	37.0	40.0	33.0	40.0
40-41	37.69983271319962	40.0	37.0	40.0	33.0	40.0
42-43	37.65647853737808	40.0	37.0	40.0	33.0	40.0
44-45	37.59523525921234	40.0	37.0	40.0	33.0	40.0
46-47	37.55925663182289	40.0	37.0	40.0	33.0	40.0
48-49	37.555491275940135	40.0	37.0	40.0	33.0	40.0
50-51	37.40858833852167	40.0	37.0	40.0	33.0	40.0
52-53	37.324826518489246	40.0	37.0	40.0	33.0	40.0
54-55	37.18332593275184	40.0	37.0	40.0	33.0	40.0
56-57	37.05817220640033	37.0	37.0	40.0	33.0	40.0
58-59	36.90848499584255	37.0	37.0	40.0	33.0	40.0
60-61	36.790191101666714	37.0	37.0	40.0	33.0	40.0
62-63	36.63689839733992	37.0	37.0	40.0	33.0	40.0
64-65	36.485413516780596	37.0	37.0	40.0	33.0	40.0
66-67	36.26780642174799	37.0	37.0	40.0	33.0	40.0
68-69	36.043352907449304	37.0	37.0	40.0	33.0	40.0
70-71	35.7978956794641	37.0	35.0	37.0	33.0	40.0
72-73	35.596172424803115	37.0	33.0	37.0	33.0	40.0
74-75	35.380386763537075	37.0	33.0	37.0	33.0	40.0
76-77	33.68322319139301	35.0	33.0	37.0	27.0	37.0
78-79	34.889777531784404	37.0	33.0	37.0	33.0	37.0
80-81	34.965744505014044	37.0	33.0	37.0	33.0	37.0
82-83	34.8146385743179	37.0	33.0	37.0	33.0	37.0
84-85	34.68259555490424	37.0	33.0	37.0	33.0	37.0
86-87	34.57285401356083	37.0	33.0	37.0	33.0	37.0
88-89	34.405812422449415	37.0	33.0	37.0	27.0	37.0
90-91	34.418975446474256	37.0	33.0	37.0	30.0	37.0
92-93	34.359686047863725	37.0	33.0	37.0	30.0	37.0
94-95	34.336425751666766	37.0	33.0	37.0	27.0	37.0
96-97	34.347773788482556	37.0	33.0	37.0	30.0	37.0
98-99	34.34081495629829	37.0	33.0	37.0	33.0	37.0
100	34.36480710409582	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	0.0
19	4.0
20	67.0
21	355.0
22	1227.0
23	3357.0
24	7115.0
25	13320.0
26	22944.0
27	35100.0
28	51288.0
29	70399.0
30	94353.0
31	123380.0
32	162025.0
33	214935.0
34	291990.0
35	418036.0
36	707318.0
37	1563827.0
38	1670700.0
39	36895.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.342810126231726	20.664004681673187	14.560703242116984	24.4324819499781
2	14.231131377631892	20.706692883259155	42.03279284689311	23.029382892215843
3	17.563744434865054	26.444183946612597	31.129282393658457	24.86278922486388
4	12.16426084732163	18.740302690868916	37.97192963789182	31.123506823917634
5	13.254914335729314	37.70747413382851	35.647745632976935	13.389865897465237
6	29.595617842007645	37.80400173449793	18.719295560632727	13.881084862861703
7	26.117071709619662	32.70038311886596	22.44672446852005	18.73582070299433
8	24.543383820679672	36.50119993382691	21.27034840714524	17.68506783834818
9	25.02530683397478	16.73550587067534	20.48519887272539	37.75398842262449
10-11	23.976670998492743	26.457281407902688	28.746717537154176	20.81933005645039
12-13	25.088851781909344	23.82831068198195	28.478255522547958	22.604582013560748
14-15	22.36724562863339	25.4335928881128	25.588070403922675	26.611091079331135
16-17	22.214526523529706	28.94509674170413	26.214044800930502	22.626331933835655
18-19	22.243203958141876	27.556391059636674	27.761851942814207	22.438553039407243
20-21	22.98620102784218	26.783339347436	27.674449896360354	22.556009728361463
22-23	22.68766645812282	27.50608617316027	27.466973952244828	22.339273416472086
24-25	22.780889771807658	27.757559156666172	27.49876141392266	21.96278965760351
26-27	22.719908803188606	27.95187556076222	27.489501674436095	21.838713961613077
28-29	22.263729665218825	27.173261355347467	27.794556670898984	22.76845230853472
30-31	23.196128096361214	27.277329251943506	27.71688507671093	21.809657574984353
32-33	23.00932590621885	27.338402774036584	27.969779457652105	21.682491862092455
34-35	22.61021678991319	27.50057854898082	28.233868752450352	21.655335908655644
36-37	22.736413253495364	27.075955865845714	27.822440259534737	22.365190621124185
38-39	22.291888431457192	26.864925352503732	28.248534237401717	22.594651978637355
40-41	22.2766494666412	26.72990847876208	28.530357510499638	22.463084544097082
42-43	22.56882946670543	26.651659470005395	28.47030360593395	22.309207457355225
44-45	22.150240412446514	27.074097410857444	28.207079069254338	22.568583107441707
46-47	22.846302870685385	26.62608606553489	27.468449784352305	23.059161279427418
48-49	22.484659145768468	26.70463442450407	27.80211134511019	23.008595084617276
50-51	22.266123927845957	27.13040038958886	27.87441651693466	22.72905916563053
52-53	22.367597274117664	27.292452203177405	28.126090563175786	22.213859959529145
54-55	22.331303987816124	27.325146494111163	27.569485443458557	22.774064074614152
56-57	22.74878954633576	27.613832073819534	27.083667498596633	22.55371088124808
58-59	22.153511047355696	27.898100216327855	27.773302570063958	22.1750861662525
60-61	22.62247034025799	28.347534685216868	26.882393845380076	22.147601129145063
62-63	22.28618207386622	28.592895442794603	26.902526485183888	22.218395998155295
64-65	22.380097530899544	28.19487379365315	26.985697116877088	22.43933155857021
66-67	22.53405108355901	27.897908918655613	26.959925866316926	22.608114131468454
68-69	22.544903037672892	27.64682841917422	27.162854463182907	22.64541407996998
70-71	22.55661491684866	27.382174899438144	27.261047182593888	22.80016300111931
72-73	22.62781473820416	27.394064523738255	27.105157843704724	22.872962894352863
74-75	22.57605813373987	27.561125851132566	27.066793892924217	22.79602212220334
76-77	22.686126534761865	27.47937358254477	27.03324991527854	22.801249967414822
78-79	22.632270317044746	27.461552947290595	27.009279788544088	22.896896947120577
80-81	22.62239846862594	27.534878742631502	27.00704253384379	22.83568025489877
82-83	22.713958123419438	27.482011127883137	27.115055774489903	22.68897497420752
84-85	22.740357540524663	27.5349111196595	26.99432313318158	22.730408206634255
86-87	22.60719920277613	27.481946927362543	27.148276108052094	22.76257776180923
88-89	22.686966528728878	27.460658065704468	27.10559789196747	22.74677751359918
90-91	22.712976127135867	27.545267148717677	27.084684558958212	22.65707216518824
92-93	22.76109597247353	27.51734533079519	27.087938896209646	22.633619800521632
94-95	22.586343348879296	27.524539500149807	27.215012933746035	22.674104217224862
96-97	22.70700516759692	27.590706846913744	27.254198292075742	22.448089693413596
98-99	23.837153177294372	29.47258887812893	27.482218812523495	19.2080391320532
100	22.72357732606827	30.498777185683412	21.29870988091721	25.47893560733111
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	917.0
1	821.0
2	1085.0
3	1504.0
4	2012.5
5	3348.5
6	4445.0
7	5060.0
8	5381.5
9	5231.0
10	4896.5
11	4558.0
12	5014.5
13	6725.5
14	10873.0
15	16795.5
16	21933.5
17	25309.0
18	26294.0
19	24860.0
20	22164.0
21	19556.0
22	17924.5
23	18261.0
24	20881.5
25	25892.0
26	33139.0
27	41641.0
28	51037.5
29	62416.0
30	74569.5
31	86888.0
32	100743.0
33	115084.5
34	129557.5
35	143327.0
36	157580.0
37	170449.0
38	180273.0
39	188691.5
40	196305.5
41	202146.0
42	205630.0
43	210445.5
44	215351.0
45	219633.0
46	223909.0
47	228701.5
48	247860.0
49	256879.5
50	246358.0
51	242978.5
52	239556.0
53	225098.5
54	204014.0
55	184976.5
56	168163.5
57	151913.5
58	135156.0
59	116229.0
60	96758.5
61	79134.5
62	62821.5
63	48118.0
64	35297.5
65	25753.0
66	19078.5
67	14289.5
68	10904.0
69	8153.5
70	5807.0
71	4002.0
72	2764.5
73	1888.5
74	1248.5
75	807.5
76	508.0
77	313.0
78	214.5
79	156.5
80	107.5
81	67.0
82	44.5
83	29.0
84	20.5
85	16.5
86	12.0
87	9.5
88	7.5
89	5.5
90	4.5
91	3.5
92	3.0
93	2.5
94	1.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	2.9151140647694617E-4
7	0.0
8	0.0
9	0.0
10-11	0.0034343687575565224
12-13	1.8219462904809136E-5
14-15	0.0024596274921492335
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	82.0
20-21	346.0
22-23	715.0
24-25	1623.0
26-27	2570.0
28-29	4151.0
30-31	9809.0
32-33	12351.0
34-35	16811.0
36-37	25356.0
38-39	28260.0
40-41	29834.0
42-43	34930.0
44-45	41440.0
46-47	40408.0
48-49	37979.0
50-51	33229.0
52-53	33370.0
54-55	43848.0
56-57	50542.0
58-59	48970.0
60-61	53378.0
62-63	53716.0
64-65	53575.0
66-67	60357.0
68-69	42108.0
70-71	39196.0
72-73	37865.0
74-75	39252.0
76-77	38157.0
78-79	41701.0
80-81	43292.0
82-83	41429.0
84-85	42147.0
86-87	45224.0
88-89	48543.0
90-91	51909.0
92-93	55743.0
94-95	68653.0
96-97	194846.0
98-99	339393.0
100-101	3601528.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.72927558793856
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.07926338883208	79.90317798185292
2	6.586821779858178	11.557142063476283
3	1.4161842584372077	3.7272245727521462
4	0.4475489241956586	1.5705257163938544
5	0.19201924213127672	0.8422854505560939
6	0.10292235526212694	0.5417582201370512
7	0.050901411713814966	0.3125880783239478
8	0.03256361653234642	0.22854259911249372
9	0.021364579001456774	0.1686869135135178
>10	0.0680868341444875	0.9163995791415755
>50	0.0016518759238076286	0.09494110817267082
>100	6.415943175812996E-4	0.12094226248296992
>500	3.013965001605615E-5	0.015785454084424166
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	1.8219462904809136E-5	0.0	0.0	0.0
4	0.0	7.287785161923654E-5	0.0	0.0	0.0
5	1.8219462904809136E-5	7.287785161923654E-5	0.0	1.8219462904809136E-5	0.0
6	1.8219462904809136E-5	7.287785161923654E-5	0.0	1.8219462904809136E-5	0.0
7	1.8219462904809136E-5	7.287785161923654E-5	0.0	1.8219462904809136E-5	0.0
8	1.8219462904809136E-5	7.287785161923654E-5	0.0	1.8219462904809136E-5	0.0
9	1.8219462904809136E-5	1.2753624033366395E-4	0.0	5.465838871442741E-5	0.0
10-11	1.8219462904809136E-5	1.2753624033366395E-4	0.0	1.0931677742885481E-4	0.0
12-13	1.8219462904809136E-5	1.2753624033366395E-4	0.0	1.6397516614328223E-4	0.0
14-15	1.8219462904809136E-5	1.9130436050049593E-4	0.0	1.9130436050049593E-4	0.0
16-17	1.8219462904809136E-5	2.1863355485770964E-4	0.0	2.277432863101142E-4	0.0
18-19	1.8219462904809136E-5	2.277432863101142E-4	0.0	2.3685301776251878E-4	0.0
20-21	1.8219462904809136E-5	2.3685301776251878E-4	0.0	2.9151140647694617E-4	0.0
22-23	1.8219462904809136E-5	2.550724806673279E-4	0.0	3.2795033228656445E-4	1.8219462904809136E-5
24-25	1.8219462904809136E-5	2.6418221211973244E-4	0.0	3.8260872100099187E-4	3.643892580961827E-5
26-27	2.7329194357213702E-5	2.7329194357213703E-4	0.0	5.101449613346558E-4	3.643892580961827E-5
28-29	3.643892580961827E-5	3.006211379293507E-4	0.0	0.0013755694493130899	3.643892580961827E-5
30-31	3.643892580961827E-5	3.461697951913736E-4	0.0	0.0043635613657017885	3.643892580961827E-5
32-33	3.643892580961827E-5	3.461697951913736E-4	0.0	0.01108654317757636	5.465838871442741E-5
34-35	5.4658388714427404E-5	4.00828183905801E-4	0.0	0.02005962865819486	5.465838871442741E-5
36-37	7.287785161923654E-5	4.372671097154193E-4	0.0	0.03411594428925511	5.465838871442741E-5
38-39	7.287785161923654E-5	4.7370603552503756E-4	0.0	0.06498882418145419	5.465838871442741E-5
40-41	7.287785161923654E-5	5.28364424239465E-4	0.0	0.09717350540279954	5.465838871442741E-5
42-43	7.287785161923654E-5	5.739130815014877E-4	0.0	0.11835363102964015	5.465838871442741E-5
44-45	8.198758307164111E-5	6.650103960255335E-4	0.0	0.14364224554151522	5.465838871442741E-5
46-47	9.109731452404568E-5	7.287785161923655E-4	0.0	0.1677192657702205	6.376812016683197E-5
48-49	9.109731452404568E-5	7.469979790971747E-4	0.0	0.19001077863425447	9.109731452404568E-5
50-51	9.109731452404568E-5	8.01656367811602E-4	0.0	0.20964224991418634	9.109731452404568E-5
52-53	9.109731452404568E-5	8.107660992640066E-4	0.0	0.2523760001574162	1.0020704597645025E-4
54-55	9.109731452404568E-5	8.198758307164111E-4	0.0	0.30157765973185324	1.0931677742885482E-4
56-57	9.109731452404568E-5	8.380952936212203E-4	0.0	0.34909401898759546	1.0931677742885482E-4
58-59	9.109731452404568E-5	8.380952936212203E-4	0.0	0.37999422807415173	1.0931677742885482E-4
60-61	9.109731452404568E-5	8.380952936212203E-4	0.0	0.40220375335511405	1.0931677742885482E-4
62-63	9.109731452404568E-5	8.563147565260294E-4	0.0	0.4182277709798937	1.2753624033366395E-4
64-65	9.109731452404568E-5	9.838509968596934E-4	0.0	0.4237300487771461	1.2753624033366395E-4
66-67	9.109731452404568E-5	9.92960728312098E-4	0.0	0.42806628094849064	1.3664597178606852E-4
68-69	1.0931677742885482E-4	0.001011180191216907	0.0	0.43256648828597855	1.4575570323847309E-4
70-71	1.0931677742885482E-4	0.0010202899226693116	0.0	0.43659298958794135	1.4575570323847309E-4
72-73	1.0931677742885482E-4	0.0010202899226693116	0.0	0.43940789660673435	1.6397516614328223E-4
74-75	1.0931677742885482E-4	0.00105672884847893	0.0	0.4418493046359788	1.6397516614328223E-4
76-77	1.0931677742885482E-4	0.001138716431550571	0.0	0.4442087250821516	1.6397516614328223E-4
78-79	1.0931677742885482E-4	0.0011660456259077847	0.0	0.4458029280863224	1.6397516614328223E-4
80-81	1.0931677742885482E-4	0.0011660456259077847	0.0	0.44640417036218105	1.730848975956868E-4
82-83	1.0931677742885482E-4	0.0011660456259077847	0.0	0.44683232774044407	1.8219462904809136E-4
84-85	1.0931677742885482E-4	0.0011842650888125939	0.0	0.4470145223694922	1.8219462904809136E-4
86-87	1.0931677742885482E-4	0.001202484551717403	0.0	0.4471056196840162	1.8219462904809136E-4
88	1.0931677742885482E-4	0.0012389234775270212	0.0	0.447123839146921	1.8219462904809136E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6685	0.0	48.350273	1
CTTATAC	9975	0.0	47.27605	1
TATACAC	12275	0.0	40.228985	3
TTATACA	12270	0.0	39.44395	2
GTATCAA	11945	0.0	35.9357	1
TCAACGC	13970	0.0	30.08401	4
ATCAACG	14060	0.0	29.92184	3
CAACGCA	14230	0.0	29.446613	5
TACACAT	17395	0.0	28.85744	5
AACGCAG	14680	0.0	28.573084	6
TATCAAC	14955	0.0	28.531374	2
ACACATC	18070	0.0	26.691015	6
ACATCTC	19050	0.0	25.362823	8
ACGCAGA	16725	0.0	25.10496	7
ATACACA	20465	0.0	24.944288	4
CACATCT	19435	0.0	24.816397	7
CGCAGAG	17315	0.0	24.249521	8
CATCTCC	21985	0.0	21.821493	9
GCAGAGT	20340	0.0	20.412039	9
TGGTATC	3245	0.0	20.158363	2
>>END_MODULE