FastQCFastQC Report
Fri 27 May 2016
SRR2139512_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139512_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5758436
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA71550.049.5177651
GTATCAA128150.037.391231
TCAACGC154000.030.6945274
ATCAACG157800.030.0372523
CAACGCA158650.029.877365
AACGCAG163450.029.1056986
TATCAAC164150.028.9803242
CTTATAC54650.027.5925641
ACGCAGA186000.025.5073347
CGCAGAG193550.024.534888
TATACAC74850.022.8016283
TTATACA74250.022.1710032
GTACATG185100.021.4985921
GCAGAGT223400.021.1414749
TACATGG188850.020.7742632
ACATGGG191650.019.8622133
GAGTACT118850.019.77785312-13
GTGGTAT38100.019.6190471
CAGAGTA221150.018.6208110-11
AGAGTAC201600.018.51892910-11
Produced by FastQC (version 0.11.5)