FastQCFastQC Report
Fri 27 May 2016
SRR2139511_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139511_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6999906
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA96700.052.539121
CTTATAC68750.044.3642271
GTATCAA174650.038.0310251
TATACAC91650.035.8608253
TTATACA94450.033.2946782
TCAACGC211500.030.8556424
ATCAACG216100.030.2784633
TATCAAC219500.030.0838362
CAACGCA217500.029.987035
AACGCAG223250.029.2339576
TACACAT124250.026.4539475
ACGCAGA255200.025.523417
ACACATC123550.024.897566
CGCAGAG265250.024.573288
ACATCTC127950.024.4455628
GTGGTAT42600.022.5192871
ATACACA159700.022.1963544
GTACATG252700.022.04571
CACATCT142800.021.6617837
TACATGG255650.021.4547652
Produced by FastQC (version 0.11.5)