##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139511_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6999906
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.50693466455121	33.0	33.0	33.0	33.0	33.0
2	32.54251557092338	33.0	33.0	33.0	33.0	33.0
3	32.56113939244327	33.0	33.0	33.0	33.0	33.0
4	36.467125558543216	37.0	37.0	37.0	37.0	37.0
5	36.49148459993606	37.0	37.0	37.0	37.0	37.0
6	36.48201875853761	37.0	37.0	37.0	37.0	37.0
7	36.45766028858102	37.0	37.0	37.0	37.0	37.0
8	36.45074505286214	37.0	37.0	37.0	37.0	37.0
9	36.49163317335976	37.0	37.0	37.0	37.0	37.0
10-11	36.41178710114107	37.0	37.0	37.0	37.0	37.0
12-13	36.41307411842388	37.0	37.0	37.0	37.0	37.0
14-15	38.379699955970835	40.0	37.0	40.0	37.0	40.0
16-17	38.32937763735684	40.0	37.0	40.0	37.0	40.0
18-19	38.33022543445583	40.0	37.0	40.0	37.0	40.0
20-21	38.324293671468396	40.0	37.0	40.0	37.0	40.0
22-23	38.32331690022945	40.0	37.0	40.0	37.0	40.0
24-25	38.30591630890292	40.0	37.0	40.0	37.0	40.0
26-27	38.19046165264473	40.0	37.0	40.0	37.0	40.0
28-29	38.12087170105266	40.0	37.0	40.0	35.0	40.0
30-31	37.98449218819621	40.0	37.0	40.0	33.0	40.0
32-33	37.99033689458746	40.0	37.0	40.0	33.0	40.0
34-35	37.938051450899515	40.0	37.0	40.0	33.0	40.0
36-37	37.8598642452215	40.0	37.0	40.0	33.0	40.0
38-39	37.7957954890132	40.0	37.0	40.0	33.0	40.0
40-41	37.81337131698717	40.0	37.0	40.0	33.0	40.0
42-43	37.75082592147538	40.0	37.0	40.0	33.0	40.0
44-45	37.68274676848365	40.0	37.0	40.0	33.0	40.0
46-47	37.644620348156906	40.0	37.0	40.0	33.0	40.0
48-49	37.620755803862735	40.0	37.0	40.0	33.0	40.0
50-51	37.51802231220595	40.0	37.0	40.0	33.0	40.0
52-53	37.418235038872545	40.0	37.0	40.0	33.0	40.0
54-55	37.25034545188712	40.0	37.0	40.0	33.0	40.0
56-57	37.13137812847825	37.0	37.0	40.0	33.0	40.0
58-59	36.967060905735764	37.0	37.0	40.0	33.0	40.0
60-61	36.839856809545694	37.0	37.0	40.0	33.0	40.0
62-63	36.66888475259212	37.0	37.0	40.0	33.0	40.0
64-65	36.497374557009266	37.0	37.0	40.0	33.0	40.0
66-67	36.26987948326705	37.0	37.0	40.0	33.0	40.0
68-69	36.04296933948501	37.0	37.0	40.0	33.0	40.0
70-71	35.79588273192	37.0	35.0	37.0	33.0	40.0
72-73	35.586902747080956	37.0	33.0	37.0	33.0	40.0
74-75	35.37251858842928	37.0	33.0	37.0	33.0	40.0
76-77	33.66327065013272	35.0	33.0	37.0	27.0	37.0
78-79	34.88331026033253	37.0	33.0	37.0	33.0	37.0
80-81	34.96766803347632	37.0	33.0	37.0	33.0	37.0
82-83	34.822414568325456	37.0	33.0	37.0	33.0	37.0
84-85	34.69467489569935	37.0	33.0	37.0	33.0	37.0
86-87	34.58355416942453	37.0	33.0	37.0	33.0	37.0
88-89	34.42060902680011	37.0	33.0	37.0	30.0	37.0
90-91	34.429461432552614	37.0	33.0	37.0	30.0	37.0
92-93	34.363010284299904	37.0	33.0	37.0	30.0	37.0
94-95	34.338745384307586	37.0	33.0	37.0	27.0	37.0
96-97	34.3485510031081	37.0	33.0	37.0	33.0	37.0
98-99	34.341073002034506	37.0	33.0	37.0	33.0	37.0
100	34.360002666890615	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	3.0
19	10.0
20	78.0
21	342.0
22	1209.0
23	3388.0
24	7836.0
25	15525.0
26	26429.0
27	41394.0
28	61197.0
29	84939.0
30	114767.0
31	151913.0
32	200217.0
33	268274.0
34	365861.0
35	528612.0
36	907987.0
37	2047455.0
38	2126224.0
39	46246.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.47859985548377	20.165656510244567	14.451836924667274	23.903906709604385
2	14.648868141943622	20.835122643075493	41.52027184365047	22.995737371330417
3	17.903897566624465	26.360982561765827	30.663454623533514	25.071665248076187
4	12.427495454938967	18.372661004304916	38.17962698356235	31.02021655719377
5	13.407565758740189	37.81480779884758	35.34017456805849	13.437451874353743
6	29.943992017023113	37.57152071566788	18.706437739423233	13.778049527885775
7	26.699272818806424	32.64012402452261	22.495587797893286	18.165015358777676
8	24.92474898948643	36.30423037109355	20.913966558979507	17.85705408044051
9	25.80284649536722	16.69246701312846	20.509618272016798	36.99506821948752
10-11	24.42418904944325	26.486792987356623	28.51840261360837	20.570615349591765
12-13	25.467315370847608	23.965044660972826	28.4303276466597	22.13731232151986
14-15	22.60620698404281	25.33637021324257	25.9069066683734	26.150516134341217
16-17	22.16730481809327	28.69572105682562	26.50515592637958	22.631818198701524
18-19	22.261498940128625	27.526405354586192	28.35668078971346	21.855414915571725
20-21	23.170244405525423	26.605475162645813	28.189238739440825	22.03504169238794
22-23	22.8335447612617	26.8550996944951	27.980737317931837	22.33061822631137
24-25	22.61147224965097	27.099785222201422	28.205447896735176	22.083294631412432
26-27	22.599491695743595	27.236903232077488	28.209478244835122	21.954126827343796
28-29	22.387762802513684	26.813255204961134	28.466762991211027	22.33221900131416
30-31	22.780870617753035	26.973496241354887	28.113901060803077	22.131732080089
32-33	22.5352393410126	26.878892588678927	28.264038628733314	22.321829441575158
34-35	22.376060202194587	27.179816529848072	28.496218534920686	21.94790473303666
36-37	22.720602979060686	26.949736579521737	27.988774608268884	22.340885833148693
38-39	22.423064026003576	26.896048791982768	28.235352325169583	22.445534856844073
40-41	22.523140287444168	26.789207148985355	28.247561768424024	22.440090795146457
42-43	22.612474186351378	26.781648084404413	28.14025924217068	22.465618487073527
44-45	22.448104343394274	27.076267088008127	27.952115692227242	22.523512876370365
46-47	22.868179369057582	26.82489089021341	27.389496701283594	22.917433039445413
48-49	22.6835741080992	26.923342798777416	27.48554879057296	22.907534302550424
50-51	22.53897799663747	27.141270788434756	27.575740118148296	22.744011096779474
52-53	22.744179377371207	27.17196572431506	27.585795783402922	22.498059114910806
54-55	22.715219889316653	27.160383155014582	27.319532628166115	22.804864327502646
56-57	22.881221130797233	27.42578792823494	27.01092957647438	22.682061364493443
58-59	22.539831047649823	27.613899137135878	27.41502966440475	22.431240150809543
60-61	22.858726851490584	27.813573881477087	26.909455721369547	22.418243545662783
62-63	22.64152376545674	27.998179882287346	26.913649561510983	22.446646790744936
64-65	22.721351900249402	27.712706345939065	26.97474425032313	22.591197503488402
66-67	22.843163957163586	27.530252504034436	26.900559090325093	22.726024448476885
68-69	22.74442707816527	27.461706703216436	27.04419696795673	22.74966925066156
70-71	22.783295924103715	27.223605976175726	27.098329510342587	22.894768589377968
72-73	22.82357363418698	27.305779383989442	26.981747467701865	22.888899514121707
74-75	22.709013305506218	27.448635603449272	26.99822920634778	22.84412188469673
76-77	22.859995247856553	27.347047422004206	26.921018686749278	22.87193864338996
78-79	22.745753803528142	27.365123023829348	26.883192086529263	23.00593108611325
80-81	22.776136713630954	27.412012293131433	26.917886006875086	22.89396498636253
82-83	22.840062504681466	27.330644863435833	27.00667883301123	22.822613798871476
84-85	22.896800117862714	27.347928712562304	26.904646138446196	22.850625031128782
86-87	22.74476014908027	27.36033216130536	27.049950397976264	22.844957291638103
88-89	22.82882498046138	27.317719124627114	26.970249451658024	22.883206443253485
90-91	22.821178381822996	27.415440401021403	26.97154345670037	22.791837760455234
92-93	22.847345251711673	27.376451344269253	26.977817631661942	22.798385772357133
94-95	22.705896462667297	27.34716200915851	27.064017814465906	22.882923713708287
96-97	22.81689691612847	27.4567804413116	27.08324675428232	22.64307588827761
98-99	23.9683332755523	29.38241933937927	27.23935826346927	19.40988912159916
100	22.860808610637605	30.42744828785836	21.048519672699012	25.663223428805026
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1498.0
1	1322.0
2	1674.5
3	2323.0
4	3122.5
5	5099.0
6	6635.5
7	7562.5
8	8266.0
9	8224.0
10	7581.0
11	6912.0
12	7624.5
13	10240.5
14	16582.0
15	25563.0
16	33014.5
17	37700.5
18	39124.0
19	36895.0
20	32298.5
21	27185.5
22	23639.0
23	23197.5
24	25787.5
25	31630.0
26	40344.0
27	50574.0
28	62090.5
29	76259.0
30	91144.5
31	105578.5
32	122630.0
33	140803.5
34	159359.0
35	176899.5
36	195555.0
37	212763.0
38	224813.5
39	236360.5
40	248475.0
41	258175.0
42	263293.5
43	269925.5
44	276861.5
45	283088.0
46	294122.5
47	306252.0
48	308700.5
49	308471.0
50	309171.0
51	299439.5
52	284597.0
53	267848.0
54	250128.5
55	233703.5
56	215279.0
57	194954.5
58	174981.0
59	152935.0
60	128119.0
61	104143.0
62	82424.0
63	63256.5
64	46773.5
65	34419.5
66	26033.5
67	19839.5
68	14820.0
69	10601.5
70	7328.5
71	4959.0
72	3162.0
73	1955.5
74	1217.5
75	769.5
76	507.0
77	331.5
78	211.5
79	145.5
80	110.0
81	75.0
82	53.0
83	36.0
84	21.0
85	14.5
86	11.0
87	8.0
88	5.5
89	4.5
90	3.5
91	2.5
92	1.5
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	1.5714496737527617E-4
7	0.0
8	0.0
9	0.0
10-11	0.0033500449863183876
12-13	3.571476531256277E-5
14-15	0.002542891290254469
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	111.0
20-21	385.0
22-23	889.0
24-25	2086.0
26-27	2716.0
28-29	4936.0
30-31	11808.0
32-33	14584.0
34-35	20794.0
36-37	30252.0
38-39	36454.0
40-41	38034.0
42-43	46450.0
44-45	55224.0
46-47	54054.0
48-49	48129.0
50-51	42792.0
52-53	41642.0
54-55	49225.0
56-57	55616.0
58-59	54355.0
60-61	56710.0
62-63	56574.0
64-65	59037.0
66-67	61828.0
68-69	50426.0
70-71	48647.0
72-73	47224.0
74-75	48506.0
76-77	47574.0
78-79	51935.0
80-81	53608.0
82-83	52163.0
84-85	52119.0
86-87	55890.0
88-89	60284.0
90-91	65352.0
92-93	69618.0
94-95	85878.0
96-97	250057.0
98-99	436333.0
100-101	4679607.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.09644577462664
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.24712800537586	77.69956962630633
2	7.023845441503844	12.094562563675886
3	1.5667426208661086	4.0467291330058615
4	0.541528171995239	1.8649460358248315
5	0.2537858707757662	1.092503073080608
6	0.12228043601770122	0.631674655733743
7	0.07236673016793338	0.43613627818533607
8	0.04362419383409678	0.3004710431119293
9	0.029689145839749898	0.2300516941398309
>10	0.09533775799196266	1.2963855497283916
>50	0.0028748081141249707	0.16605330983472286
>100	7.443130752973537E-4	0.10434663893958737
>500	2.6252242962803666E-5	0.013041497169332951
>1k	2.62521994079494E-5	0.02352890126365641
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	2.8571812250050214E-5	0.0	0.0	0.0
3	0.0	2.8571812250050214E-5	0.0	0.0	0.0
4	0.0	1.0000134287517575E-4	0.0	0.0	0.0
5	0.0	1.0000134287517575E-4	0.0	0.0	0.0
6	8.571543675015064E-5	1.0000134287517575E-4	0.0	0.0	0.0
7	8.571543675015064E-5	1.0000134287517575E-4	0.0	0.0	0.0
8	8.571543675015064E-5	1.1428724900020086E-4	0.0	0.0	0.0
9	8.571543675015064E-5	2.5714631025045194E-4	0.0	0.0	0.0
10-11	9.28583898126632E-5	2.5714631025045194E-4	0.0	7.1429530625125534E-6	0.0
12-13	1.0000134287517575E-4	2.5714631025045194E-4	0.0	1.4285906125025107E-5	7.1429530625125534E-6
14-15	1.1428724900020086E-4	3.7857651231316535E-4	0.0	2.8571812250050214E-5	3.571476531256277E-5
16-17	2.000026857503515E-4	3.857194653756779E-4	0.0	4.285771837507532E-5	5.714362450010043E-5
18-19	2.000026857503515E-4	4.285771837507532E-4	0.0	5.714362450010043E-5	5.714362450010043E-5
20-21	2.000026857503515E-4	4.7143490212582853E-4	0.0	5.714362450010043E-5	5.714362450010043E-5
22-23	2.000026857503515E-4	5.571503388759792E-4	0.0	5.714362450010043E-5	7.142953062512554E-5
24-25	2.000026857503515E-4	6.357228225636173E-4	0.0	5.714362450010043E-5	1.0000134287517575E-4
26-27	2.000026857503515E-4	6.7143758787618E-4	0.0	9.28583898126632E-5	1.1428724900020086E-4
28-29	2.000026857503515E-4	6.7143758787618E-4	0.0	2.642892633129645E-4	1.1428724900020086E-4
30-31	2.000026857503515E-4	8.142966491264312E-4	0.0	6.57151681751155E-4	1.1428724900020086E-4
32-33	2.000026857503515E-4	8.285825552514563E-4	0.0	0.001728594641128038	1.2857315512522597E-4
34-35	2.1428859187537662E-4	9.428698042516571E-4	0.0	0.003364330892443413	1.2857315512522597E-4
36-37	2.1428859187537662E-4	0.0010643000063143705	0.0	0.005614361107134867	1.2857315512522597E-4
38-39	2.1428859187537662E-4	0.0010857288655019082	0.0	0.008621544346452653	1.4285906125025108E-4
40-41	2.285744980004017E-4	0.0012143020206271341	0.0	0.012221592689958979	1.5714496737527617E-4
42-43	2.285744980004017E-4	0.0012571597390022096	0.0	0.015564494723214854	1.5714496737527617E-4
44-45	2.285744980004017E-4	0.0014214476594399982	0.0	0.019364545752471533	1.5714496737527617E-4
46-47	2.285744980004017E-4	0.0015428778615027116	0.0	0.02393603571247957	1.5714496737527617E-4
48-49	2.285744980004017E-4	0.0015428778615027116	0.0	0.028243236409174637	1.5714496737527617E-4
50-51	2.285744980004017E-4	0.0018214530309407012	0.0	0.03245043576299453	1.5714496737527617E-4
52-53	2.285744980004017E-4	0.0018285959840032137	0.0	0.036536204914751715	1.7857382656281384E-4
54-55	2.285744980004017E-4	0.0018285959840032137	0.0	0.04104340829719713	2.000026857503515E-4
56-57	2.285744980004017E-4	0.0018714537023782891	0.0	0.045522039867392505	2.000026857503515E-4
58-59	2.285744980004017E-4	0.0018714537023782891	0.0	0.05022210298252577	2.000026857503515E-4
60-61	2.5714631025045194E-4	0.001921454373815877	0.0	0.05469359159965862	2.000026857503515E-4
62-63	2.8571812250050216E-4	0.001978597998315977	0.0	0.05907936478004133	2.000026857503515E-4
64-65	2.8571812250050216E-4	0.002214315449378892	0.0	0.06330799299304876	2.000026857503515E-4
66-67	2.8571812250050216E-4	0.002242887261628942	0.0	0.06774376684486906	2.0714563881286407E-4
68-69	2.8571812250050216E-4	0.00232145974531658	0.0	0.07216525479056433	2.1428859187537662E-4
70-71	2.928610755630147E-4	0.0023500315575666303	0.0	0.07613673669332131	2.1428859187537662E-4
72-73	3.2857584087557745E-4	0.0023786033698166803	0.0	0.0795439253041398	2.5714631025045194E-4
74-75	3.2857584087557745E-4	0.0024214610881917557	0.0	0.08217967498420693	2.5714631025045194E-4
76-77	3.2857584087557745E-4	0.0025071765249419066	0.0	0.08482971057039909	2.5714631025045194E-4
78-79	3.2857584087557745E-4	0.002542891290254469	0.0	0.08661544883602723	2.5714631025045194E-4
80-81	3.2857584087557745E-4	0.002542891290254469	0.0	0.08720117098715326	2.7143221637547705E-4
82-83	3.2857584087557745E-4	0.002542891290254469	0.0	0.08764403407702903	3.1428993475055234E-4
84-85	3.2857584087557745E-4	0.002542891290254469	0.0	0.08786546562196693	3.1428993475055234E-4
86-87	3.2857584087557745E-4	0.0025857490086295446	0.0	0.08794403810565456	3.1428993475055234E-4
88	3.2857584087557745E-4	0.0027143221637547705	0.0	0.08794403810565456	3.1428993475055234E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9670	0.0	52.53912	1
CTTATAC	6875	0.0	44.364227	1
GTATCAA	17465	0.0	38.031025	1
TATACAC	9165	0.0	35.860825	3
TTATACA	9445	0.0	33.294678	2
TCAACGC	21150	0.0	30.855642	4
ATCAACG	21610	0.0	30.278463	3
TATCAAC	21950	0.0	30.083836	2
CAACGCA	21750	0.0	29.98703	5
AACGCAG	22325	0.0	29.233957	6
TACACAT	12425	0.0	26.453947	5
ACGCAGA	25520	0.0	25.52341	7
ACACATC	12355	0.0	24.89756	6
CGCAGAG	26525	0.0	24.57328	8
ACATCTC	12795	0.0	24.445562	8
GTGGTAT	4260	0.0	22.519287	1
ATACACA	15970	0.0	22.196354	4
GTACATG	25270	0.0	22.0457	1
CACATCT	14280	0.0	21.661783	7
TACATGG	25565	0.0	21.454765	2
>>END_MODULE