FastQCFastQC Report
Fri 27 May 2016
SRR2139510_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139510_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8796391
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC123700.058.2178041
GGTATCA106550.048.1917151
TATACAC164150.046.1561623
TTATACA160000.045.8618932
GTATCAA189950.036.993051
TACACAT238500.031.6800825
TCAACGC226700.030.4513954
ATCAACG231800.029.7810753
CAACGCA233950.029.5092335
TATCAAC240300.028.9227092
AACGCAG241350.028.6221086
ACACATC255900.028.3935726
ATACACA282400.027.9460244
ACATCTC264600.027.78298
CACATCT281550.025.7008887
ACGCAGA274600.025.1253597
CGCAGAG284450.024.2568
CATCTCC306850.023.7221959
GTACATG277400.022.1204951
TACATGG284050.021.2125762
Produced by FastQC (version 0.11.5)