##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139510_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8796391
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.50073956466919	33.0	33.0	33.0	33.0	33.0
2	32.547615152623386	33.0	33.0	33.0	33.0	33.0
3	32.56993487442748	33.0	33.0	33.0	33.0	33.0
4	36.47326124998309	37.0	37.0	37.0	37.0	37.0
5	36.496361860221995	37.0	37.0	37.0	37.0	37.0
6	36.48329627457443	37.0	37.0	37.0	37.0	37.0
7	36.45951674953967	37.0	37.0	37.0	37.0	37.0
8	36.45340617532804	37.0	37.0	37.0	37.0	37.0
9	36.49873101366231	37.0	37.0	37.0	37.0	37.0
10-11	36.4177555886272	37.0	37.0	37.0	37.0	37.0
12-13	36.41972594215059	37.0	37.0	37.0	37.0	37.0
14-15	38.38845055887124	40.0	37.0	40.0	37.0	40.0
16-17	38.33085591579547	40.0	37.0	40.0	37.0	40.0
18-19	38.32832914089426	40.0	37.0	40.0	37.0	40.0
20-21	38.311338973711074	40.0	37.0	40.0	37.0	40.0
22-23	38.31115934383436	40.0	37.0	40.0	37.0	40.0
24-25	38.29796968428228	40.0	37.0	40.0	37.0	40.0
26-27	38.170448711647495	40.0	37.0	40.0	37.0	40.0
28-29	38.09773294848567	40.0	37.0	40.0	33.0	40.0
30-31	37.96241172672738	40.0	37.0	40.0	33.0	40.0
32-33	37.977549812410004	40.0	37.0	40.0	33.0	40.0
34-35	37.93720047112265	40.0	37.0	40.0	33.0	40.0
36-37	37.84641750126177	40.0	37.0	40.0	33.0	40.0
38-39	37.76473436315812	40.0	37.0	40.0	33.0	40.0
40-41	37.78446703351874	40.0	37.0	40.0	33.0	40.0
42-43	37.73126933084693	40.0	37.0	40.0	33.0	40.0
44-45	37.669906569384736	40.0	37.0	40.0	33.0	40.0
46-47	37.63522267231801	40.0	37.0	40.0	33.0	40.0
48-49	37.6188731533658	40.0	37.0	40.0	33.0	40.0
50-51	37.48357242386783	40.0	37.0	40.0	33.0	40.0
52-53	37.403166920723905	40.0	37.0	40.0	33.0	40.0
54-55	37.25919414718621	40.0	37.0	40.0	33.0	40.0
56-57	37.14485577784197	38.5	37.0	40.0	33.0	40.0
58-59	36.99885159604888	37.0	37.0	40.0	33.0	40.0
60-61	36.86864396866997	37.0	37.0	40.0	33.0	40.0
62-63	36.71940308406661	37.0	37.0	40.0	33.0	40.0
64-65	36.56718062904632	37.0	37.0	40.0	33.0	40.0
66-67	36.351457456091836	37.0	37.0	40.0	33.0	40.0
68-69	36.129940520043526	37.0	37.0	40.0	33.0	40.0
70-71	35.88292184220023	37.0	35.0	37.0	33.0	40.0
72-73	35.67774773132275	37.0	33.0	37.0	33.0	40.0
74-75	35.456953152850076	37.0	33.0	37.0	33.0	40.0
76-77	33.757961873859415	35.0	33.0	37.0	27.0	37.0
78-79	34.96228738571479	37.0	33.0	37.0	33.0	37.0
80-81	35.03647178746073	37.0	33.0	37.0	33.0	37.0
82-83	34.88872245192604	37.0	33.0	37.0	33.0	37.0
84-85	34.759431616988124	37.0	33.0	37.0	33.0	37.0
86-87	34.64599112286686	37.0	33.0	37.0	33.0	37.0
88-89	34.48081493725577	37.0	33.0	37.0	33.0	37.0
90-91	34.488024417736725	37.0	33.0	37.0	33.0	37.0
92-93	34.4215383210374	37.0	33.0	37.0	33.0	37.0
94-95	34.39836368474964	37.0	33.0	37.0	33.0	37.0
96-97	34.4057784919	37.0	33.0	37.0	33.0	37.0
98-99	34.404469354991065	37.0	33.0	37.0	33.0	37.0
100	34.421637231569285	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	2.0
19	7.0
20	77.0
21	470.0
22	1752.0
23	4864.0
24	10477.0
25	19941.0
26	33417.0
27	52604.0
28	76737.0
29	105774.0
30	142811.0
31	189191.0
32	251029.0
33	332717.0
34	455373.0
35	656776.0
36	1117616.0
37	2525853.0
38	2755191.0
39	63711.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.42479466863171	20.590069268180553	14.579877133701766	24.405258929485967
2	14.182395939425612	20.759229552210673	42.07058326534143	22.98779124302228
3	17.486569207758045	26.559324159192105	31.238834199161907	24.715272433887943
4	12.07963584156275	18.846740668985724	38.1483155989769	30.925307890474627
5	13.18274733353713	37.78997545698003	35.721854565127906	13.305422644354941
6	29.3952967015977	37.91885980369197	18.90134350721547	13.784499987494856
7	26.03845145128269	32.811274532930604	22.556193784473656	18.594080231313047
8	24.554945317915042	36.397859076523545	21.412599780978358	17.63459582458306
9	25.17747335242374	16.704714467558343	20.46601839322513	37.65179378679279
10-11	23.94245440855999	26.47857089627302	28.891558763093112	20.687415932073883
12-13	25.061593449130836	23.887287853312554	28.57700715861848	22.474111538938132
14-15	22.32680230011118	25.505891276597403	25.71372573116511	26.453580692126305
16-17	22.17135413830513	28.965242677366206	26.32618309031511	22.53722009401356
18-19	22.472716367428415	27.57447344030069	27.861642348549537	22.09116784372136
20-21	23.027741529554213	26.872837884333567	27.721350423787317	22.378070162324903
22-23	22.465471823915724	27.256261407328765	27.827876338033086	22.45039043072243
24-25	22.62203560562372	27.225325897249014	27.81930067216885	22.333337824958413
26-27	22.643557178630978	27.520921959012757	27.888155665096466	21.947365197259796
28-29	22.131704260373553	26.895771978945955	28.210002657729955	22.762521102950537
30-31	22.348265642873557	27.00348298106273	28.352513791961435	22.295737584102273
32-33	21.775118366292393	26.845956693725242	28.853376433510547	22.525548506471814
34-35	21.895591419338462	27.035023222713956	28.412393668614545	22.656991689333044
36-37	22.173214946725395	27.117250050123687	28.386875991675538	22.32265901147538
38-39	22.22919390048593	26.87391799018149	28.320389643810056	22.576498465522526
40-41	22.219934892901364	26.795082709866914	28.55542334289371	22.42955905433801
42-43	22.506565922279833	26.656500729884076	28.531138063629456	22.305795284206635
44-45	22.090131737565912	27.16108231531884	28.257682614584013	22.491103332531235
46-47	22.85420798774158	26.644119493970468	27.49911757503694	23.002554943251006
48-49	22.469834088607335	26.712268829558756	27.850779799374774	22.967117282459135
50-51	22.223998955224825	27.13306409509139	27.94277087496611	22.70016607471767
52-53	22.350409573283088	27.317796190050924	28.18228659487771	22.14950764178828
54-55	22.295060722375712	27.374320099751582	27.63924172041306	22.691377457459648
56-57	22.733596094785153	27.695000667002244	27.088997350625128	22.48240588758748
58-59	22.15849711593958	27.994298474687962	27.799219709882927	22.047984699489533
60-61	22.649301618161616	28.373587494451975	26.919428788023104	22.05768209936331
62-63	22.302514663945097	28.609017880174182	26.942666854946996	22.145800600933722
64-65	22.38487076994622	28.17445116345974	27.095364360644936	22.345313705949106
66-67	22.55937607538763	27.876206676658764	27.055097357747115	22.50931989020649
68-69	22.532392618394994	27.678426614756603	27.219871285005638	22.569309481842765
70-71	22.523296524609293	27.4311411840127	27.344807728727428	22.70075456265058
72-73	22.629076634008857	27.478526372299022	27.222809018923634	22.669587974768486
74-75	22.49218301590759	27.61480511380594	27.211589265448765	22.68142260483771
76-77	22.630192391637895	27.519244929881932	27.165220514814454	22.685342163665716
78-79	22.56131663470792	27.504286156892032	27.125950682918145	22.808446525481905
80-81	22.55383332219311	27.57912671878437	27.148660248324553	22.71837971069796
82-83	22.62711732425018	27.521542770626823	27.228059920870823	22.62327998425217
84-85	22.68391483426308	27.484074428134353	27.1635907289671	22.66842000863546
86-87	22.53656885792798	27.51743982808761	27.256450290554145	22.689541023430273
88-89	22.633580798937526	27.497504589524404	27.195995548264325	22.672919063273753
90-91	22.613550703007338	27.553745311328793	27.201293070581322	22.63141091508255
92-93	22.668167331423355	27.491734735545954	27.22601463444205	22.614083298588643
94-95	22.491236678701227	27.515703451608157	27.29820351913375	22.69485635055686
96-97	22.605312958422676	27.609474691453833	27.322402699553777	22.462809650569714
98-99	23.77284286892519	29.506384058283032	27.523544649006887	19.197228423784896
100	22.63469865419393	30.582706377171736	21.291872072890524	25.490722895743804
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1296.0
1	1198.0
2	1694.0
3	2425.5
4	3273.5
5	5415.0
6	7198.5
7	8269.0
8	8773.5
9	8419.5
10	7736.5
11	7107.0
12	7901.0
13	10742.5
14	17367.0
15	26783.0
16	35036.5
17	40424.5
18	41849.5
19	39699.5
20	35617.0
21	31154.0
22	28420.5
23	28964.5
24	32837.5
25	40683.0
26	52152.0
27	65707.0
28	81384.5
29	100087.5
30	119781.0
31	140005.0
32	163319.0
33	187346.5
34	211072.0
35	234526.5
36	259337.5
37	281240.0
38	296314.0
39	310246.0
40	324280.5
41	334275.0
42	339632.0
43	347125.0
44	354554.0
45	360370.0
46	365686.0
47	370933.0
48	399141.0
49	412639.0
50	395737.0
51	388337.0
52	382675.5
53	361102.0
54	326264.0
55	293103.5
56	264429.5
57	238091.0
58	211015.5
59	180388.0
60	148466.0
61	119614.5
62	93715.0
63	71272.5
64	52354.0
65	37989.5
66	28058.5
67	21250.0
68	16061.0
69	11633.0
70	8073.5
71	5561.0
72	3678.0
73	2307.5
74	1466.5
75	932.5
76	616.0
77	413.5
78	279.0
79	193.0
80	128.5
81	85.5
82	61.0
83	41.5
84	29.5
85	24.0
86	20.0
87	17.0
88	11.5
89	5.5
90	2.5
91	1.0
92	0.0
93	0.5
94	1.0
95	1.0
96	0.5
97	0.0
98	0.5
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	1.2505128523732063E-4
7	0.0
8	0.0
9	0.0
10-11	0.003461646941342194
12-13	1.1368298657938238E-5
14-15	0.0023589219715221843
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	96.0
20-21	459.0
22-23	1106.0
24-25	2435.0
26-27	3465.0
28-29	6222.0
30-31	15321.0
32-33	19597.0
34-35	27287.0
36-37	40963.0
38-39	47413.0
40-41	49914.0
42-43	58504.0
44-45	69607.0
46-47	67214.0
48-49	63877.0
50-51	58291.0
52-53	59575.0
54-55	78461.0
56-57	87069.0
58-59	85745.0
60-61	87579.0
62-63	87267.0
64-65	86414.0
66-67	94638.0
68-69	67786.0
70-71	63809.0
72-73	61116.0
74-75	62653.0
76-77	62160.0
78-79	66964.0
80-81	69176.0
82-83	66948.0
84-85	68228.0
86-87	71827.0
88-89	77557.0
90-91	82927.0
92-93	87950.0
94-95	107491.0
96-97	304474.0
98-99	537569.0
100-101	5739237.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.01557376652147
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.28082276026991	75.90260373311456
2	7.653182843648419	13.012794611857373
3	1.765027781420726	4.501645486540004
4	0.6220601788530151	2.1153921208997617
5	0.2708494401665868	1.1513210280051749
6	0.14037015412703138	0.7160189515682774
7	0.08599374466521818	0.5117565280131688
8	0.05118902743018164	0.3481491630021678
9	0.03279516541931797	0.2509289824402171
>10	0.0951183904884785	1.2497212679687084
>50	0.0019624391139058932	0.11407849027368094
>100	5.852496190580289E-4	0.09012851336851808
>500	2.141240528722391E-5	0.013179608549593984
>1k	2.141237284711187E-5	0.02228151439879078
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	3.410489597381472E-5	0.0	0.0	0.0
3	0.0	3.410489597381472E-5	0.0	0.0	0.0
4	0.0	5.684149328969119E-5	0.0	1.1368298657938238E-5	0.0
5	0.0	5.684149328969119E-5	0.0	1.1368298657938238E-5	0.0
6	0.0	5.684149328969119E-5	0.0	2.2736597315876476E-5	0.0
7	0.0	5.684149328969119E-5	0.0	2.2736597315876476E-5	0.0
8	0.0	5.684149328969119E-5	0.0	2.2736597315876476E-5	1.1368298657938238E-5
9	0.0	1.3641958389525887E-4	0.0	3.410489597381472E-5	3.410489597381472E-5
10-11	0.0	1.3641958389525887E-4	0.0	3.978904530278383E-5	3.410489597381472E-5
12-13	5.684149328969119E-6	1.3641958389525887E-4	0.0	6.820979194762942E-5	3.410489597381472E-5
14-15	1.1368298657938238E-5	1.9894522651391918E-4	0.0	1.0231468792144414E-4	3.410489597381472E-5
16-17	1.1368298657938238E-5	2.046293758428883E-4	0.0	1.1368298657938238E-4	3.410489597381472E-5
18-19	2.2736597315876476E-5	2.2736597315876477E-4	0.0	1.9894522651391918E-4	3.410489597381472E-5
20-21	4.547319463175295E-5	2.5578671980361036E-4	0.0	2.2736597315876477E-4	3.410489597381472E-5
22-23	5.684149328969119E-5	2.955757651063942E-4	0.0	2.5578671980361036E-4	4.547319463175295E-5
24-25	6.820979194762943E-5	3.524172583960854E-4	0.0	2.7283916779051773E-4	5.684149328969119E-5
26-27	7.957809060556767E-5	3.524172583960854E-4	0.0	3.126282130933016E-4	6.252564261866031E-5
28-29	8.52622399345368E-5	3.524172583960854E-4	0.0	4.6041609564649865E-4	6.820979194762943E-5
30-31	9.663053859247502E-5	3.524172583960854E-4	0.0	0.0010401993272013488	6.820979194762943E-5
32-33	1.0231468792144414E-4	3.524172583960854E-4	0.0	0.002245238984942802	6.820979194762943E-5
34-35	1.1368298657938238E-4	4.2631119967268395E-4	0.0	0.0038368007970541558	6.820979194762943E-5
36-37	1.2505128523732063E-4	5.002051409492825E-4	0.0	0.006349194800458506	6.820979194762943E-5
38-39	1.2505128523732063E-4	5.058892902782516E-4	0.0	0.009822210040458638	6.820979194762943E-5
40-41	1.2505128523732063E-4	5.684149328969119E-4	0.0	0.013630590090867948	7.957809060556767E-5
42-43	1.2505128523732063E-4	5.740990822258811E-4	0.0	0.017365076200000662	7.957809060556767E-5
44-45	1.3641958389525887E-4	6.082039781996958E-4	0.0	0.021730502884648943	8.52622399345368E-5
46-47	1.3641958389525887E-4	6.423088741735106E-4	0.0	0.02607319297198135	9.663053859247502E-5
48-49	1.3641958389525887E-4	6.479930235024796E-4	0.0	0.03000662430762798	1.2505128523732063E-4
50-51	1.3641958389525887E-4	7.218869647790782E-4	0.0	0.033905950747300795	1.2505128523732063E-4
52-53	1.3641958389525887E-4	7.275711141080472E-4	0.0	0.03795874921885578	1.5347203188216622E-4
54-55	1.3641958389525887E-4	7.275711141080472E-4	0.0	0.042068389183700455	1.818927785270118E-4
56-57	1.5347203188216622E-4	7.275711141080472E-4	0.0	0.0458881375327677	1.818927785270118E-4
58-59	1.705244798690736E-4	7.275711141080472E-4	0.0	0.05010577633486279	1.818927785270118E-4
60-61	1.705244798690736E-4	7.332552634370164E-4	0.0	0.054096049163799106	1.818927785270118E-4
62-63	1.705244798690736E-4	7.446235620949546E-4	0.0	0.05812611103803822	1.818927785270118E-4
64-65	1.762086291980427E-4	9.435687886088738E-4	0.0	0.062070910672342786	1.818927785270118E-4
66-67	1.818927785270118E-4	9.54937087266812E-4	0.0	0.06627718117577994	1.818927785270118E-4
68-69	1.818927785270118E-4	0.001000410281898565	0.0	0.07061987126311234	1.818927785270118E-4
70-71	1.818927785270118E-4	0.0010174627298854724	0.0	0.07470109048131217	1.9326107718495005E-4
72-73	1.818927785270118E-4	0.0010345151778723798	0.0	0.07781600431358723	2.1599767450082653E-4
74-75	1.818927785270118E-4	0.001091356671162071	0.0	0.0804761861995448	2.1599767450082653E-4
76-77	1.818927785270118E-4	0.0011766189110966078	0.0	0.08323868277342378	2.2168182382979566E-4
78-79	1.818927785270118E-4	0.0012050396577414533	1.1368298657938238E-5	0.0849496117214435	2.2736597315876477E-4
80-81	1.818927785270118E-4	0.0012107238070704226	1.1368298657938238E-5	0.08558055229695906	2.2736597315876477E-4
82-83	1.818927785270118E-4	0.0012164079563993916	1.1368298657938238E-5	0.08615465137918493	2.2736597315876477E-4
84-85	1.9326107718495005E-4	0.0012164079563993916	1.1368298657938238E-5	0.08635359660569886	2.2736597315876477E-4
86-87	1.9326107718495005E-4	0.0012732494496890828	1.1368298657938238E-5	0.08645022714429135	2.38734271816703E-4
88	1.9326107718495005E-4	0.001284617748347021	1.1368298657938238E-5	0.08646727959227825	2.5010257047464125E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	12370	0.0	58.217804	1
GGTATCA	10655	0.0	48.191715	1
TATACAC	16415	0.0	46.156162	3
TTATACA	16000	0.0	45.861893	2
GTATCAA	18995	0.0	36.99305	1
TACACAT	23850	0.0	31.680082	5
TCAACGC	22670	0.0	30.451395	4
ATCAACG	23180	0.0	29.781075	3
CAACGCA	23395	0.0	29.509233	5
TATCAAC	24030	0.0	28.922709	2
AACGCAG	24135	0.0	28.622108	6
ACACATC	25590	0.0	28.393572	6
ATACACA	28240	0.0	27.946024	4
ACATCTC	26460	0.0	27.7829	8
CACATCT	28155	0.0	25.700888	7
ACGCAGA	27460	0.0	25.125359	7
CGCAGAG	28445	0.0	24.256	8
CATCTCC	30685	0.0	23.722195	9
GTACATG	27740	0.0	22.120495	1
TACATGG	28405	0.0	21.212576	2
>>END_MODULE