FastQCFastQC Report
Fri 27 May 2016
SRR2139509_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139509_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9954263
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC114300.050.305181
GGTATCA128800.046.9809651
TATACAC156250.039.057883
TTATACA155200.037.963322
GTATCAA230650.036.4046021
TCAACGC273250.029.8470554
ATCAACG277150.029.473643
CAACGCA279200.029.1974835
TATCAAC287250.028.7070062
AACGCAG286400.028.5386096
TACACAT224250.027.2352145
ACACATC228900.025.2340836
ACGCAGA328550.024.916667
ACATCTC239650.024.4258028
CGCAGAG336850.024.2647488
ATACACA283750.022.8727284
CACATCT253900.022.7155327
GTACATG326150.022.6967431
TACATGG324800.022.0622942
ACATGGG332850.021.011513
Produced by FastQC (version 0.11.5)