##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139509_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9954263
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.503120421873525	33.0	33.0	33.0	33.0	33.0
2	32.54491769003893	33.0	33.0	33.0	33.0	33.0
3	32.56415125861151	33.0	33.0	33.0	33.0	33.0
4	36.467041809122385	37.0	37.0	37.0	37.0	37.0
5	36.490377740672514	37.0	37.0	37.0	37.0	37.0
6	36.478203660080105	37.0	37.0	37.0	37.0	37.0
7	36.45420359096399	37.0	37.0	37.0	37.0	37.0
8	36.44756874516978	37.0	37.0	37.0	37.0	37.0
9	36.49017129645861	37.0	37.0	37.0	37.0	37.0
10-11	36.406603331658005	37.0	37.0	37.0	37.0	37.0
12-13	36.40824925963881	37.0	37.0	37.0	37.0	37.0
14-15	38.37358421211093	40.0	37.0	40.0	37.0	40.0
16-17	38.319590762269385	40.0	37.0	40.0	37.0	40.0
18-19	38.32123734323676	40.0	37.0	40.0	37.0	40.0
20-21	38.32141145929051	40.0	37.0	40.0	37.0	40.0
22-23	38.317719858247855	40.0	37.0	40.0	37.0	40.0
24-25	38.300154019090726	40.0	37.0	40.0	37.0	40.0
26-27	38.18875782257845	40.0	37.0	40.0	37.0	40.0
28-29	38.120782345927275	40.0	37.0	40.0	33.0	40.0
30-31	37.992021852627076	40.0	37.0	40.0	33.0	40.0
32-33	37.98632186675272	40.0	37.0	40.0	33.0	40.0
34-35	37.92662714102917	40.0	37.0	40.0	33.0	40.0
36-37	37.855482780559	40.0	37.0	40.0	33.0	40.0
38-39	37.78720209639898	40.0	37.0	40.0	33.0	40.0
40-41	37.804536506797675	40.0	37.0	40.0	33.0	40.0
42-43	37.74211288818146	40.0	37.0	40.0	33.0	40.0
44-45	37.67875913160031	40.0	37.0	40.0	33.0	40.0
46-47	37.643905787045455	40.0	37.0	40.0	33.0	40.0
48-49	37.63032965836719	40.0	37.0	40.0	33.0	40.0
50-51	37.523497256645925	40.0	37.0	40.0	33.0	40.0
52-53	37.43195787543611	40.0	37.0	40.0	33.0	40.0
54-55	37.26439786734876	40.0	37.0	40.0	33.0	40.0
56-57	37.15158850631815	37.0	37.0	40.0	33.0	40.0
58-59	36.993464196424085	37.0	37.0	40.0	33.0	40.0
60-61	36.86748223575712	37.0	37.0	40.0	33.0	40.0
62-63	36.71779508692529	37.0	37.0	40.0	33.0	40.0
64-65	36.55678286178214	37.0	37.0	40.0	33.0	40.0
66-67	36.333586039950326	37.0	37.0	40.0	33.0	40.0
68-69	36.109474056510166	37.0	37.0	40.0	33.0	40.0
70-71	35.86221089824049	37.0	35.0	37.0	33.0	40.0
72-73	35.656886735317045	37.0	33.0	37.0	33.0	40.0
74-75	35.442974974993696	37.0	33.0	37.0	33.0	40.0
76-77	33.74467960906284	35.0	33.0	37.0	27.0	37.0
78-79	34.9476388089282	37.0	33.0	37.0	33.0	37.0
80-81	35.02180834531619	37.0	33.0	37.0	33.0	37.0
82-83	34.867649374955235	37.0	33.0	37.0	33.0	37.0
84-85	34.73311942426141	37.0	33.0	37.0	33.0	37.0
86-87	34.616657260547605	37.0	33.0	37.0	33.0	37.0
88-89	34.44961834822528	37.0	33.0	37.0	30.0	37.0
90-91	34.457055127902734	37.0	33.0	37.0	33.0	37.0
92-93	34.389251103042156	37.0	33.0	37.0	33.0	37.0
94-95	34.36473789465302	37.0	33.0	37.0	33.0	37.0
96-97	34.37039320714452	37.0	33.0	37.0	33.0	37.0
98-99	34.37372474422876	37.0	33.0	37.0	33.0	37.0
100	34.38820826598145	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	11.0
20	87.0
21	466.0
22	1717.0
23	4969.0
24	11208.0
25	22037.0
26	38066.0
27	60001.0
28	86810.0
29	121018.0
30	162172.0
31	215065.0
32	284456.0
33	379308.0
34	516548.0
35	748991.0
36	1273896.0
37	2865834.0
38	3096474.0
39	65127.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.82461956249298	20.619286430346477	14.522873265454209	24.033220741706344
2	14.195978145242897	20.783567804065452	42.18210830877183	22.83834574191982
3	17.630014396846857	26.57066625625624	30.925694850537905	24.873624496359
4	12.0440558984628	18.63537260367744	38.521003513770935	30.799567984088828
5	13.114793129335641	38.015792831674226	35.70790725541409	13.16150678357604
6	29.498910465269656	38.09368240637742	18.862839376431108	13.544567751921818
7	26.25650939702919	32.79215146314699	22.686219964250494	18.265119175573318
8	24.532082385205214	36.73243312940396	21.153479669966526	17.582004815424305
9	25.295252898180408	16.797416343128567	20.613349275581726	37.29398148310929
10-11	24.11878905523803	26.605756389398138	28.83282638953527	20.442628165828562
12-13	25.16293400197835	23.97659273634641	28.71378039584156	22.146692865833682
14-15	22.263154397068796	25.513993092525965	25.992122463727018	26.230730046678218
16-17	21.99399895301139	28.985651675066247	26.557204687077284	22.463144684845076
18-19	22.00340195954236	27.765184624918994	28.389932032135377	21.84148138340327
20-21	22.725940468313205	26.777279002246672	28.198791777431953	22.297988752008166
22-23	22.4770704648961	27.163857845397054	28.121065875698736	22.2380058140081
24-25	22.364260816863546	27.343313169377282	28.246408061388887	22.046017952370285
26-27	22.18168353425974	27.34902024598645	28.37585087815797	22.093445341595835
28-29	21.846200611142443	27.0366630162491	28.716480202425725	22.400656170182728
30-31	22.296209039857622	26.937657790613635	28.092145013357435	22.673988156171312
32-33	22.389742335612492	27.087583149899856	28.574412431567787	21.94826208291986
34-35	22.337771895432628	27.539845738618745	28.400593294355502	21.72178907159313
36-37	22.151286710811686	27.24237070741547	28.05991804109638	22.546424540676462
38-39	22.163788190274584	27.083301105253515	28.404389464471087	22.348521240000814
40-41	22.241632164633046	27.0089098591738	28.48845621891537	22.26100175727778
42-43	22.400837136661007	26.939943971459922	28.42061889772138	22.238599994157692
44-45	22.153608655301447	27.304176942134635	28.16994109680855	22.372273305755368
46-47	22.647002556493614	26.9635603536322	27.602373345104308	22.787063744769878
48-49	22.39530380644781	27.03965643842421	27.798604357296348	22.766435397831636
50-51	22.252929692666644	27.335627708147058	27.850624031151295	22.560818568035003
52-53	22.370596578877695	27.402661847741655	28.02409363129847	22.202647942082184
54-55	22.32587238650082	27.468211740447067	27.639051763380085	22.56686410967203
56-57	22.63315540099626	27.770495657072914	27.190952336560976	22.405396605369855
58-59	22.18492582707582	28.04716354863606	27.72255189117201	22.045358733116107
60-61	22.53544976665367	28.334446182960622	27.081383187456233	22.04872086292948
62-63	22.288853029749816	28.509080079786646	27.065643588915044	22.13642330154849
64-65	22.3420506262822	28.17189501552383	27.16436073621339	22.321693621980586
66-67	22.481120826990757	27.912656080937303	27.134038145208393	22.47218494686355
68-69	22.477416868829266	27.69719861392861	27.331374126228564	22.494010391013557
70-71	22.46982837331552	27.521779680990672	27.381513890848165	22.626878054845648
72-73	22.515797567157506	27.566948756008276	27.310112728164388	22.607140948669834
74-75	22.44081873271245	27.69554825116835	27.277468656626873	22.586164359492326
76-77	22.52790791964437	27.63193576801932	27.24795075639468	22.59220555594163
78-79	22.445218215746273	27.60501092925019	27.219876185350987	22.729894669652545
80-81	22.462751657891186	27.68711446650631	27.21421759078658	22.635916284815924
82-83	22.55464204800801	27.603268909118732	27.318968021570196	22.523121021303066
84-85	22.597883530651686	27.56776956259422	27.276031715273145	22.55831519148095
86-87	22.442792244390173	27.59868328597147	27.355395923970526	22.60312854566783
88-89	22.507630990159246	27.590398170653387	27.28318578343173	22.61878505575564
90-91	22.5199912150855	27.661952364228792	27.268178383072588	22.54987803761312
92-93	22.586509045183032	27.577921064894284	27.311857826107204	22.523712063815477
94-95	22.376091612935323	27.61497016877702	27.398216764230938	22.61072145405672
96-97	22.490445665435647	27.72256766156041	27.43022783678311	22.356758836220834
98-99	23.644758231293824	29.58031721399458	27.62730757844404	19.147616976267553
100	22.572797886423636	30.632554552225873	21.404529181784916	25.39011837956558
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1220.0
1	1172.0
2	1922.5
3	2849.5
4	3905.0
5	6581.0
6	8709.5
7	9805.5
8	10340.5
9	9920.0
10	9021.5
11	8198.5
12	9048.5
13	12407.0
14	20558.5
15	32026.5
16	41682.5
17	48046.5
18	49970.5
19	47471.0
20	42611.0
21	37402.0
22	34022.5
23	34464.0
24	39186.5
25	48624.0
26	61940.0
27	77402.0
28	95911.5
29	117850.5
30	139708.0
31	162005.5
32	188252.5
33	215110.0
34	241693.0
35	268661.5
36	295730.0
37	318657.0
38	335313.5
39	350139.0
40	364959.5
41	376942.0
42	383715.5
43	391577.0
44	398993.0
45	403812.5
46	407480.5
47	411913.5
48	437367.5
49	450321.5
50	432766.0
51	422767.5
52	411947.5
53	382208.0
54	346645.0
55	317423.0
56	289819.5
57	260607.0
58	229227.5
59	196536.0
60	162931.0
61	131150.0
62	103309.0
63	79678.5
64	59115.5
65	42771.0
66	31496.5
67	23884.0
68	18154.0
69	13290.5
70	9488.5
71	6623.5
72	4572.5
73	3042.0
74	1961.5
75	1258.0
76	823.0
77	497.5
78	308.5
79	215.0
80	157.5
81	115.5
82	87.5
83	63.0
84	38.5
85	23.0
86	20.0
87	19.0
88	14.5
89	8.5
90	5.0
91	3.5
92	3.0
93	3.0
94	2.0
95	0.5
96	0.0
97	0.5
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	1.406432600786216E-4
7	0.0
8	0.0
9	0.0
10-11	0.003340277426867263
12-13	2.5114867871182425E-5
14-15	0.002637061126474155
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	128.0
20-21	523.0
22-23	1182.0
24-25	2419.0
26-27	3638.0
28-29	6584.0
30-31	15406.0
32-33	19114.0
34-35	26978.0
36-37	40096.0
38-39	49962.0
40-41	51757.0
42-43	61219.0
44-45	73133.0
46-47	70883.0
48-49	65326.0
50-51	59630.0
52-53	59616.0
54-55	72433.0
56-57	82391.0
58-59	81827.0
60-61	86957.0
62-63	88801.0
64-65	88538.0
66-67	92886.0
68-69	69426.0
70-71	68329.0
72-73	64580.0
74-75	66340.0
76-77	64660.0
78-79	71112.0
80-81	73921.0
82-83	72152.0
84-85	71705.0
86-87	76020.0
88-89	82540.0
90-91	90032.0
92-93	95970.0
94-95	118944.0
96-97	346848.0
98-99	622226.0
100-101	6698031.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	80.73007811779888
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.28778419069087	70.46749636444044
2	8.358501986751191	13.495650366764014
3	2.2971743306802814	5.563531894980643
4	0.8845927315195696	2.856529612720477
5	0.44133724189567936	1.7814595007266045
6	0.23783754568565285	1.1520386185528986
7	0.14090827823902008	0.796287541677644
8	0.08415850963618553	0.5435298445765429
9	0.058904878094815524	0.42798558691024885
>10	0.20327148575580695	2.526438608797923
>50	0.0043998751590793445	0.23509575125907275
>100	0.001104454178934395	0.1380594766737682
>500	2.4491712959480973E-5	0.01589683191967915
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	3.0137841445418913E-5	0.0	2.0091894296945943E-5	0.0
3	0.0	3.0137841445418913E-5	0.0	3.0137841445418913E-5	0.0
4	0.0	7.03216300393108E-5	0.0	3.0137841445418913E-5	0.0
5	0.0	7.03216300393108E-5	0.0	3.0137841445418913E-5	0.0
6	0.0	7.03216300393108E-5	0.0	3.0137841445418913E-5	0.0
7	0.0	7.03216300393108E-5	0.0	3.0137841445418913E-5	0.0
8	0.0	9.041352433625674E-5	0.0	3.0137841445418913E-5	1.0045947148472972E-5
9	0.0	1.6073515437556754E-4	0.0	3.0137841445418913E-5	3.0137841445418913E-5
10-11	0.0	1.6073515437556754E-4	0.0	5.0229735742364856E-5	3.0137841445418913E-5
12-13	0.0	1.707811015240405E-4	0.0	6.0275682890837826E-5	3.0137841445418913E-5
14-15	0.0	2.9133246730571615E-4	0.0	6.0275682890837826E-5	3.0137841445418913E-5
16-17	0.0	3.1142436160266207E-4	0.0	7.03216300393108E-5	3.0137841445418913E-5
18-19	0.0	3.365392294738445E-4	0.0	1.356202865043851E-4	3.0137841445418913E-5
20-21	0.0	3.717000444934999E-4	0.0	1.4566623365285807E-4	3.0137841445418913E-5
22-23	0.0	4.118838330873918E-4	0.0	1.6073515437556754E-4	4.0183788593891886E-5
24-25	0.0	4.671365424039932E-4	0.0	1.6073515437556754E-4	5.525270931660134E-5
26-27	0.0	5.022973574236486E-4	0.0	1.707811015240405E-4	6.0275682890837826E-5
28-29	0.0	5.07320330997885E-4	0.0	2.7626354658300673E-4	6.0275682890837826E-5
30-31	0.0	5.927108817599053E-4	0.0	6.881473796703985E-4	6.0275682890837826E-5
32-33	0.0	6.077798024826148E-4	0.0	0.0017530177774085333	7.03216300393108E-5
34-35	0.0	7.383771154127634E-4	0.0	0.003074059827432729	9.041352433625674E-5
36-37	0.0	8.338136133232565E-4	0.0	0.005218869543631708	9.041352433625674E-5
38-39	0.0	8.438595604717295E-4	0.0	0.008182423952431234	1.0045947148472971E-4
40-41	0.0	8.940892962140944E-4	0.0	0.011683436533674065	1.0045947148472971E-4
42-43	0.0	9.292501112337498E-4	0.0	0.015013668013392854	1.0045947148472971E-4
44-45	0.0	0.001054824450589662	0.0	0.018645277907565833	1.1050541863320267E-4
46-47	0.0	0.0011251460806289727	0.0	0.022849506789201772	1.2557433935591215E-4
48-49	0.0	0.0011452379749259185	0.0	0.027119034327302782	1.3059731293014863E-4
50-51	0.0	0.001240674472836412	0.0	0.031192665896008574	1.3059731293014863E-4
52-53	0.0	0.0012557433935591214	0.0	0.035386848830496045	1.456662336528581E-4
54-55	0.0	0.0012657893407075943	0.0	0.03921937766763848	1.6575812794980403E-4
56-57	0.0	0.0012758352878560672	0.0	0.043564249809353035	1.75804075098277E-4
58-59	0.0	0.0012959271821530133	0.0	0.047964374660384196	1.8082704867251347E-4
60-61	0.0	0.0013260650235984322	0.0	0.05203298325551575	1.9087299582098643E-4
62-63	0.0	0.0013612258386180875	0.0	0.056217120242854746	1.9087299582098643E-4
64-65	0.0	0.0015269839665678915	0.0	0.060325912626580186	1.9087299582098643E-4
66-67	0.0	0.001552098834439074	0.0	0.06435936040669209	2.0091894296945942E-4
68-69	0.0	0.0015872596494587296	0.0	0.0687795771520202	2.109648901179324E-4
70-71	0.0	0.0016123745173299117	0.0	0.07258196814771722	2.1598786369216887E-4
72-73	0.0	0.0016324664116268578	0.0	0.07564598202800146	2.511486787118243E-4
74-75	0.0	0.0016726502002207496	0.0	0.0781273309736743	2.511486787118243E-4
76-77	0.0	0.0017881785924281888	0.0	0.08069909344368337	2.511486787118243E-4
78-79	0.0	0.0018183164338736078	0.0	0.0823667206703299	2.511486787118243E-4
80-81	0.0	0.0018233394074478443	0.0	0.08305486805000029	2.511486787118243E-4
82-83	0.0	0.0018283623810220807	0.0	0.08352702756597852	2.6119462586029726E-4
84-85	0.0	0.001833385354596317	0.0	0.08373799245609645	2.6119462586029726E-4
86-87	0.0	0.001893661037487155	0.0	0.08381333705971	2.6119462586029726E-4
88	0.0	0.0019991434825461212	0.0	0.08381333705971	2.6119462586029726E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	11430	0.0	50.30518	1
GGTATCA	12880	0.0	46.980965	1
TATACAC	15625	0.0	39.05788	3
TTATACA	15520	0.0	37.96332	2
GTATCAA	23065	0.0	36.404602	1
TCAACGC	27325	0.0	29.847055	4
ATCAACG	27715	0.0	29.47364	3
CAACGCA	27920	0.0	29.197483	5
TATCAAC	28725	0.0	28.707006	2
AACGCAG	28640	0.0	28.538609	6
TACACAT	22425	0.0	27.235214	5
ACACATC	22890	0.0	25.234083	6
ACGCAGA	32855	0.0	24.91666	7
ACATCTC	23965	0.0	24.425802	8
CGCAGAG	33685	0.0	24.264748	8
ATACACA	28375	0.0	22.872728	4
CACATCT	25390	0.0	22.715532	7
GTACATG	32615	0.0	22.696743	1
TACATGG	32480	0.0	22.062294	2
ACATGGG	33285	0.0	21.01151	3
>>END_MODULE