FastQCFastQC Report
Fri 27 May 2016
SRR2139508_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139508_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8429410
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA157400.053.7764971
CTTATAC78550.037.8203351
GTATCAA287100.037.3277931
TATACAC108550.030.248753
ATCAACG358850.029.5934623
TCAACGC360000.029.4645884
TATCAAC366950.029.0208382
CAACGCA367950.028.8181935
TTATACA108900.028.4053042
AACGCAG376650.028.1749846
ACGCAGA427050.024.8399057
TGGTATC61850.024.80182
GTGGTAT64600.024.5309871
CGCAGAG433050.024.5056488
GTACATG446700.022.1084881
TACATGG446700.021.8057612
GCAGAGT489850.021.5616159
ACATCTC138300.021.3911118
ACACATC140400.021.161346
ACATGGG454200.020.9432773
Produced by FastQC (version 0.11.5)