##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139508_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8429410
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.51497186635838	33.0	33.0	33.0	33.0	33.0
2	32.55407246770533	33.0	33.0	33.0	33.0	33.0
3	32.57189435559547	33.0	33.0	33.0	33.0	33.0
4	36.483069514948255	37.0	37.0	37.0	37.0	37.0
5	36.51250834874564	37.0	37.0	37.0	37.0	37.0
6	36.50357510193477	37.0	37.0	37.0	37.0	37.0
7	36.48177369471885	37.0	37.0	37.0	37.0	37.0
8	36.46981022396585	37.0	37.0	37.0	37.0	37.0
9	36.50511376241042	37.0	37.0	37.0	37.0	37.0
10-11	36.43149384120596	37.0	37.0	37.0	37.0	37.0
12-13	36.435312376548296	37.0	37.0	37.0	37.0	37.0
14-15	38.397483038551925	40.0	37.0	40.0	37.0	40.0
16-17	38.350966971591134	40.0	37.0	40.0	37.0	40.0
18-19	38.373865193412115	40.0	37.0	40.0	37.0	40.0
20-21	38.37967899461012	40.0	37.0	40.0	37.0	40.0
22-23	38.38356675862122	40.0	37.0	40.0	37.0	40.0
24-25	38.367826674084945	40.0	37.0	40.0	37.0	40.0
26-27	38.25336853673022	40.0	37.0	40.0	37.0	40.0
28-29	38.188697645595255	40.0	37.0	40.0	37.0	40.0
30-31	38.03791282648683	40.0	37.0	40.0	33.0	40.0
32-33	38.04198073945908	40.0	37.0	40.0	33.0	40.0
34-35	38.00371417492125	40.0	37.0	40.0	33.0	40.0
36-37	37.925364472066065	40.0	37.0	40.0	33.0	40.0
38-39	37.8756715889983	40.0	37.0	40.0	33.0	40.0
40-41	37.89738226910002	40.0	37.0	40.0	33.0	40.0
42-43	37.842709957265086	40.0	37.0	40.0	33.0	40.0
44-45	37.785642757799685	40.0	37.0	40.0	33.0	40.0
46-47	37.7525818877609	40.0	37.0	40.0	33.0	40.0
48-49	37.73854596467378	40.0	37.0	40.0	33.0	40.0
50-51	37.657379776771194	40.0	37.0	40.0	33.0	40.0
52-53	37.56719594188171	40.0	37.0	40.0	33.0	40.0
54-55	37.43515954591228	40.0	37.0	40.0	33.0	40.0
56-57	37.337596994921725	40.0	37.0	40.0	33.0	40.0
58-59	37.18773551564246	37.0	37.0	40.0	33.0	40.0
60-61	37.084478554250815	37.0	37.0	40.0	33.0	40.0
62-63	36.93675059855485	37.0	37.0	40.0	33.0	40.0
64-65	36.775918782641924	37.0	37.0	40.0	33.0	40.0
66-67	36.56159930916427	37.0	37.0	40.0	33.0	40.0
68-69	36.34424282147469	37.0	37.0	40.0	33.0	40.0
70-71	36.098718168973086	37.0	37.0	38.5	33.0	40.0
72-73	35.89436310286307	37.0	37.0	37.0	33.0	40.0
74-75	35.67366690501609	37.0	33.0	37.0	33.0	40.0
76-77	33.984590367465024	35.0	33.0	37.0	27.0	37.0
78-79	35.1462059589039	37.0	33.0	37.0	33.0	37.0
80-81	35.20194635246388	37.0	33.0	37.0	33.0	37.0
82-83	35.042556821375356	37.0	33.0	37.0	33.0	37.0
84-85	34.90686551771313	37.0	33.0	37.0	33.0	37.0
86-87	34.788224426351604	37.0	33.0	37.0	33.0	37.0
88-89	34.623601558068	37.0	33.0	37.0	33.0	37.0
90-91	34.628249801253205	37.0	33.0	37.0	33.0	37.0
92-93	34.5619273714604	37.0	33.0	37.0	33.0	37.0
94-95	34.53689317998025	37.0	33.0	37.0	33.0	37.0
96-97	34.541202674173725	37.0	33.0	37.0	33.0	37.0
98-99	34.52584222615167	37.0	33.0	37.0	33.0	37.0
100	34.54303479476743	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	2.0
18	6.0
19	30.0
20	74.0
21	376.0
22	1246.0
23	3546.0
24	8028.0
25	15593.0
26	27193.0
27	43613.0
28	64200.0
29	91480.0
30	125317.0
31	167987.0
32	224284.0
33	302162.0
34	414735.0
35	598523.0
36	1025229.0
37	2374269.0
38	2863760.0
39	77757.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.63977075501133	20.77411111809723	14.631581569765856	22.95453655712559
2	14.269551486996123	21.370226385951092	42.37786511748746	21.98235700956532
3	17.66540006951851	27.75760106579227	30.94834632554354	23.62865253914568
4	11.922423989342077	18.958029091003997	39.38204453217959	29.73750238747433
5	12.652059871331446	38.87555594045135	35.473324942077795	12.99905924613941
6	28.263660010349508	38.642209527479224	19.54328583363011	13.550844628541153
7	25.768268479051322	33.66851298014926	23.122270716455837	17.440947824343578
8	24.505012806352994	36.3951213667386	21.56540018815077	17.534465638757634
9	26.073011041104895	16.674796931220573	21.084678524356985	36.16751350331755
10-11	23.882599587179367	27.103726389265642	28.886132295862023	20.127541727692964
12-13	25.18216287337888	24.859512656109736	28.919056820960243	21.03926764955115
14-15	22.256749005392564	26.043897378222503	26.91034326487492	24.78901035151002
16-17	21.249761252566906	29.38939973260287	27.428005044243903	21.932833970586316
18-19	21.342021564973113	28.16979480177142	29.519402900084344	20.968780733171126
20-21	22.654432746598218	27.00132290338321	29.33934791101443	21.004896439004142
22-23	22.176924856116777	27.369753791944795	29.121914153598155	21.331407198340273
24-25	21.781925496164472	27.64532201852613	29.296536595550805	21.276215889758593
26-27	21.698740938074877	27.814582232231594	29.215334992615137	21.27134183707839
28-29	21.37945679122018	27.73801049966987	29.429039403541697	21.453493305568255
30-31	22.068231646053153	27.56679790789194	29.205149449622642	21.15982099643227
32-33	21.347558848839416	28.00295614104471	29.224990970523407	21.424494039592467
34-35	21.63381393944233	27.938942256502024	29.332102999529848	21.0951408045258
36-37	21.50089764841772	27.918136670358614	29.108980536603603	21.471985144620067
38-39	21.449967860355702	27.61381693044002	29.362891156169376	21.573324053034902
40-41	21.62063072087152	27.518597098361226	29.35406897413667	21.506703206630583
42-43	21.731266388261606	27.504089103631703	29.13282653763035	21.631817970476337
44-45	21.63430276985415	27.863039720776445	28.780746030717726	21.721911478651688
46-47	21.937213476459366	27.704524086551448	28.27918257811074	22.079079858878448
48-49	21.858659584079977	27.80106862609959	28.251632721027313	22.08863906879312
50-51	21.776554623890224	27.94632968309093	28.28249543606054	21.994620256958306
52-53	21.86054018466377	27.980913603052798	28.360142285062306	21.798403927221127
54-55	21.87153920995601	28.006284090925927	28.06400485885179	22.058171840266276
56-57	22.08960616534976	28.1555865161317	27.773021446266284	21.98178587225226
58-59	21.745828277789236	28.351066870483276	28.125169616553542	21.777935235173945
60-61	22.052620900403195	28.541298202092836	27.69968937786024	21.706391519643738
62-63	21.86673430269529	28.6153900131913	27.73775763665646	21.780118047456952
64-65	21.886354044217153	28.39699202831898	27.78952180669879	21.927132120765073
66-67	22.004601430639553	28.26984880867028	27.68255861050558	22.04299115018459
68-69	22.025503505087496	28.193518207842068	27.778044071881702	22.002934215188738
70-71	22.002379750108766	27.986001058831544	27.869613456595772	22.14200573446392
72-73	22.041508034839303	27.983371027920118	27.825192863045263	22.14992807419532
74-75	21.989136627904905	28.091058647519446	27.755830130918106	22.163974593657542
76-77	22.000943460034872	28.037085225673724	27.733511220713247	22.228460093578153
78-79	22.053907691805964	27.949069644332813	27.744375964904417	22.25264669895681
80-81	22.002648849427654	28.059228561809054	27.71525656431021	22.22286602445308
82-83	22.150495877777175	27.95079638809655	27.792460191022318	22.106247543103954
84-85	22.207507470952322	27.87261054568836	27.738294604101295	22.181587379258023
86-87	22.068302236922076	27.963838871498435	27.783071333446372	22.18478755813311
88-89	22.201159400919344	27.830421969552393	27.738706737284435	22.229711892243824
90-91	22.191820303537263	27.795967294232366	27.810151205342976	22.202061196887392
92-93	22.183380309574048	27.760612559488973	27.866125532972685	22.189881597964295
94-95	22.091190297944692	27.749268880394485	27.882655535070565	22.276885286590257
96-97	22.150019690602566	27.929918891825388	27.913978880224878	22.00608253734717
98-99	23.287742656950403	29.78092614550107	28.177746042892537	18.75358515465599
100	22.15532863474408	30.797548565388116	21.946612143685577	25.10051065618223
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1506.0
1	1445.0
2	2225.5
3	3290.0
4	4687.5
5	7915.5
6	10388.0
7	11883.5
8	12792.5
9	12562.5
10	11789.5
11	11102.5
12	12485.5
13	17046.0
14	27680.5
15	42503.5
16	54945.0
17	62522.0
18	63724.0
19	58911.5
20	50920.5
21	42449.0
22	36488.5
23	35726.5
24	40064.5
25	49161.5
26	62759.5
27	78176.5
28	94886.5
29	114992.5
30	136677.0
31	159009.0
32	183482.5
33	207827.5
34	231862.0
35	255040.0
36	279310.0
37	300283.5
38	313639.0
39	325742.0
40	336600.0
41	341817.5
42	342446.0
43	346953.5
44	352274.0
45	356442.0
46	363561.5
47	371039.0
48	368335.0
49	358591.0
50	351853.5
51	336565.5
52	313160.0
53	289180.5
54	264344.0
55	239937.0
56	215725.0
57	189914.5
58	163935.0
59	139357.0
60	115242.5
61	92310.5
62	72319.0
63	55531.0
64	41084.5
65	29693.0
66	21521.5
67	15793.5
68	11597.0
69	8024.0
70	5330.5
71	3643.5
72	2434.0
73	1612.5
74	1111.5
75	744.5
76	476.5
77	289.5
78	202.0
79	149.0
80	111.5
81	79.5
82	55.5
83	37.5
84	23.5
85	16.5
86	13.0
87	10.0
88	8.5
89	6.5
90	4.0
91	3.0
92	2.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	2.847174357398679E-4
7	0.0
8	0.0
9	0.0
10-11	0.0038614802222219588
12-13	3.5589679467483486E-5
14-15	0.0024319614302780387
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	135.0
20-21	450.0
22-23	1026.0
24-25	2656.0
26-27	3595.0
28-29	6740.0
30-31	15542.0
32-33	20056.0
34-35	28787.0
36-37	42989.0
38-39	52801.0
40-41	55152.0
42-43	67985.0
44-45	82468.0
46-47	79646.0
48-49	70782.0
50-51	61874.0
52-53	59239.0
54-55	67198.0
56-57	73783.0
58-59	73755.0
60-61	77144.0
62-63	75685.0
64-65	77777.0
66-67	84576.0
68-69	74611.0
70-71	73955.0
72-73	70036.0
74-75	70291.0
76-77	71174.0
78-79	76686.0
80-81	78325.0
82-83	76039.0
84-85	75951.0
86-87	80126.0
88-89	85983.0
90-91	92537.0
92-93	94435.0
94-95	105357.0
96-97	286956.0
98-99	500598.0
100-101	5334509.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.49980972185543
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.85804377318843	75.08487791090722
2	7.834744013029085	13.240687566408074
3	1.8981548174528164	4.811811626921586
4	0.7043235237559005	2.3806081496000124
5	0.29499321432331826	1.2463435239779455
6	0.1455135652163278	0.7377521143637101
7	0.08061317532087077	0.4768258582981707
8	0.047985091595187615	0.3243784887423326
9	0.03714068741206787	0.2824542917332782
>10	0.09645347506824299	1.229653216064859
>50	0.001465406799344721	0.08247974698335321
>100	5.471501855738824E-4	0.08723948257081504
>500	2.2106652838205812E-5	0.014888023428571585
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.1863226489161162E-5	0.0	0.0	0.0
3	0.0	1.1863226489161162E-5	0.0	0.0	0.0
4	0.0	3.558967946748349E-5	0.0	0.0	0.0
5	0.0	3.558967946748349E-5	0.0	0.0	0.0
6	2.3726452978322325E-5	3.558967946748349E-5	0.0	0.0	0.0
7	2.3726452978322325E-5	4.745290595664465E-5	0.0	0.0	0.0
8	2.3726452978322325E-5	4.745290595664465E-5	0.0	0.0	0.0
9	2.3726452978322325E-5	1.0676903840245047E-4	0.0	0.0	0.0
10-11	2.3726452978322325E-5	1.0676903840245047E-4	0.0	0.0	0.0
12-13	2.9658066222902907E-5	1.0676903840245047E-4	0.0	0.0	0.0
14-15	5.9316132445805814E-5	1.1863226489161163E-4	0.0	0.0	0.0
16-17	1.0676903840245047E-4	1.304954913807728E-4	0.0	0.0	0.0
18-19	1.0676903840245047E-4	1.304954913807728E-4	0.0	0.0	0.0
20-21	1.1270065164703104E-4	1.5422194435909511E-4	0.0	0.0	0.0
22-23	1.304954913807728E-4	1.7794839733741746E-4	0.0	1.1863226489161162E-5	0.0
24-25	1.304954913807728E-4	2.3726452978322326E-4	0.0	1.7794839733741743E-5	0.0
26-27	1.304954913807728E-4	2.491277562723844E-4	0.0	5.9316132445805814E-5	5.931613244580581E-6
28-29	1.304954913807728E-4	2.491277562723844E-4	0.0	2.609909827615456E-4	1.1863226489161162E-5
30-31	1.304954913807728E-4	3.0844388871819023E-4	0.0	9.016052131762483E-4	1.1863226489161162E-5
32-33	1.3642710462535338E-4	3.0844388871819023E-4	0.0	0.0023311240051201686	1.1863226489161162E-5
34-35	1.4235871786993397E-4	3.558967946748349E-4	0.0	0.004247035083119697	1.1863226489161162E-5
36-37	1.4235871786993397E-4	4.152129271206407E-4	0.0	0.007254362998122051	1.1863226489161162E-5
38-39	1.4235871786993397E-4	4.508026065881242E-4	0.0	0.011969995527563613	1.1863226489161162E-5
40-41	1.5422194435909511E-4	4.863922860556077E-4	0.0	0.016922892586788398	1.7794839733741743E-5
42-43	1.5422194435909511E-4	4.923238993001883E-4	0.0	0.021585140597028735	2.3726452978322325E-5
44-45	1.5422194435909511E-4	5.51640031745994E-4	0.0	0.026567695722476425	2.3726452978322325E-5
46-47	1.5422194435909511E-4	6.050245509472193E-4	0.0	0.032321360569719584	2.3726452978322325E-5
48-49	1.5422194435909511E-4	6.109561641917999E-4	0.0	0.03803943573749527	2.3726452978322325E-5
50-51	1.8388001058199804E-4	6.52477456903864E-4	0.0	0.043852416717184234	2.3726452978322325E-5
52-53	1.898116238265786E-4	6.643406833930251E-4	0.0	0.0495764234982045	3.558967946748349E-5
54-55	1.898116238265786E-4	6.702722966376057E-4	0.0	0.055063165749441534	3.558967946748349E-5
56-57	1.898116238265786E-4	6.880671363713475E-4	0.0	0.06033043831062909	3.558967946748349E-5
58-59	1.898116238265786E-4	7.177252025942504E-4	0.0	0.06523588246389724	3.558967946748349E-5
60-61	2.1353807680490094E-4	7.355200423279922E-4	0.0	0.07036672792045943	3.558967946748349E-5
62-63	2.491277562723844E-4	7.473832688171532E-4	0.0	0.07508829206314557	3.558967946748349E-5
64-65	2.491277562723844E-4	7.829729482846367E-4	0.0	0.08002932589588121	3.558967946748349E-5
66-67	2.491277562723844E-4	7.889045615292173E-4	0.0	0.0850711971537747	4.745290595664465E-5
68-69	2.491277562723844E-4	8.126310145075397E-4	0.0	0.09049269165932136	4.745290595664465E-5
70-71	2.491277562723844E-4	8.185626277521203E-4	0.0	0.09545152033179072	4.745290595664465E-5
72-73	3.26238728451932E-4	8.244942409967008E-4	0.0	0.09937824829970307	4.745290595664465E-5
74-75	3.3217034169651257E-4	8.304258542412814E-4	0.0	0.10213051684518845	4.745290595664465E-5
76-77	3.3217034169651257E-4	8.541523072196038E-4	0.0	0.10501921249529919	4.745290595664465E-5
78-79	3.3217034169651257E-4	8.660155337087649E-4	0.0	0.1069469867997879	4.745290595664465E-5
80-81	3.3217034169651257E-4	8.660155337087649E-4	0.0	0.1076172590964255	5.9316132445805814E-5
82-83	3.3810195494109315E-4	8.660155337087649E-4	0.0	0.10810958299572568	5.9316132445805814E-5
84-85	3.4403356818567374E-4	8.660155337087649E-4	0.0	0.10834091591226433	5.9316132445805814E-5
86-87	3.499651814302543E-4	8.660155337087649E-4	0.0	0.10844175333742219	5.9316132445805814E-5
88	3.558967946748349E-4	9.016052131762484E-4	0.0	0.10844175333742219	7.117935893496698E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	15740	0.0	53.776497	1
CTTATAC	7855	0.0	37.820335	1
GTATCAA	28710	0.0	37.327793	1
TATACAC	10855	0.0	30.24875	3
ATCAACG	35885	0.0	29.593462	3
TCAACGC	36000	0.0	29.464588	4
TATCAAC	36695	0.0	29.020838	2
CAACGCA	36795	0.0	28.818193	5
TTATACA	10890	0.0	28.405304	2
AACGCAG	37665	0.0	28.174984	6
ACGCAGA	42705	0.0	24.839905	7
TGGTATC	6185	0.0	24.8018	2
GTGGTAT	6460	0.0	24.530987	1
CGCAGAG	43305	0.0	24.505648	8
GTACATG	44670	0.0	22.108488	1
TACATGG	44670	0.0	21.805761	2
GCAGAGT	48985	0.0	21.561615	9
ACATCTC	13830	0.0	21.391111	8
ACACATC	14040	0.0	21.16134	6
ACATGGG	45420	0.0	20.943277	3
>>END_MODULE