FastQCFastQC Report
Fri 27 May 2016
SRR2139507_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139507_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9377788
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA122850.049.0936781
GTATCAA210450.037.377811
TCAACGC252200.030.586714
ATCAACG256250.030.1881393
CAACGCA259400.029.7062665
TATCAAC266650.029.1411842
AACGCAG267500.028.855526
ACGCAGA310850.024.8174447
CGCAGAG320800.024.0890128
GTACATG307850.021.7664551
TACATGG308150.021.2090422
GCAGAGT373050.020.5874949
ACATGGG312700.020.3998263
GAGTACT192050.019.60890412-13
GTGGTAT61350.018.9243471
CAGAGTA358550.018.71526710-11
CATGGGG216900.018.4839844
AGAGTAC332300.018.39417510-11
TGGTATC60600.018.010482
GTACTTT214700.017.4594114-15
Produced by FastQC (version 0.11.5)