##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139507_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9377788
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.51329684569538	33.0	33.0	33.0	33.0	33.0
2	32.54802945001529	33.0	33.0	33.0	33.0	33.0
3	32.56960735303464	33.0	33.0	33.0	33.0	33.0
4	36.475016496427514	37.0	37.0	37.0	37.0	37.0
5	36.49834193308699	37.0	37.0	37.0	37.0	37.0
6	36.48894920635868	37.0	37.0	37.0	37.0	37.0
7	36.46700064023627	37.0	37.0	37.0	37.0	37.0
8	36.45900248544753	37.0	37.0	37.0	37.0	37.0
9	36.495957468861526	37.0	37.0	37.0	37.0	37.0
10-11	36.41688349107487	37.0	37.0	37.0	37.0	37.0
12-13	36.420388582040886	37.0	37.0	37.0	37.0	37.0
14-15	38.40562475927159	40.0	37.0	40.0	37.0	40.0
16-17	38.36026395563645	40.0	37.0	40.0	37.0	40.0
18-19	38.35546063741258	40.0	37.0	40.0	37.0	40.0
20-21	38.3514096143485	40.0	37.0	40.0	37.0	40.0
22-23	38.353140208650984	40.0	37.0	40.0	37.0	40.0
24-25	38.33952677827792	40.0	37.0	40.0	37.0	40.0
26-27	38.23180759607439	40.0	37.0	40.0	37.0	40.0
28-29	38.16741776040999	40.0	37.0	40.0	37.0	40.0
30-31	38.034031156761415	40.0	37.0	40.0	33.0	40.0
32-33	38.043495214781274	40.0	37.0	40.0	33.0	40.0
34-35	38.00699418237783	40.0	37.0	40.0	33.0	40.0
36-37	37.9299131521059	40.0	37.0	40.0	33.0	40.0
38-39	37.88015926654175	40.0	37.0	40.0	33.0	40.0
40-41	37.88368852541828	40.0	37.0	40.0	33.0	40.0
42-43	37.82200715460996	40.0	37.0	40.0	33.0	40.0
44-45	37.760413415802006	40.0	37.0	40.0	33.0	40.0
46-47	37.72358505733651	40.0	37.0	40.0	33.0	40.0
48-49	37.69992996982895	40.0	37.0	40.0	33.0	40.0
50-51	37.61837400840555	40.0	37.0	40.0	33.0	40.0
52-53	37.51143212431989	40.0	37.0	40.0	33.0	40.0
54-55	37.35590424483722	40.0	37.0	40.0	33.0	40.0
56-57	37.251643651898874	38.5	37.0	40.0	33.0	40.0
58-59	37.082983336205416	37.0	37.0	40.0	33.0	40.0
60-61	36.96592414503198	37.0	37.0	40.0	33.0	40.0
62-63	36.7968260196808	37.0	37.0	40.0	33.0	40.0
64-65	36.616446855234514	37.0	37.0	40.0	33.0	40.0
66-67	36.382475768329066	37.0	37.0	40.0	33.0	40.0
68-69	36.15256459705559	37.0	37.0	40.0	33.0	40.0
70-71	35.89663575474265	37.0	35.0	37.0	33.0	40.0
72-73	35.68480853953645	37.0	33.0	37.0	33.0	40.0
74-75	35.46158632682943	37.0	33.0	37.0	33.0	40.0
76-77	33.76469437436504	35.0	33.0	37.0	27.0	37.0
78-79	34.95636427127536	37.0	33.0	37.0	33.0	37.0
80-81	35.024734076096514	37.0	33.0	37.0	33.0	37.0
82-83	34.87258612610668	37.0	33.0	37.0	33.0	37.0
84-85	34.74236888328335	37.0	33.0	37.0	33.0	37.0
86-87	34.624096014880855	37.0	33.0	37.0	33.0	37.0
88-89	34.45768458308421	37.0	33.0	37.0	30.0	37.0
90-91	34.460774185803515	37.0	33.0	37.0	33.0	37.0
92-93	34.390743400191	37.0	33.0	37.0	33.0	37.0
94-95	34.36508895841554	37.0	33.0	37.0	33.0	37.0
96-97	34.372731288829016	37.0	33.0	37.0	33.0	37.0
98-99	34.375659852120336	37.0	33.0	37.0	33.0	37.0
100	34.38918792619895	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	2.0
18	2.0
19	9.0
20	73.0
21	374.0
22	1472.0
23	4079.0
24	9647.0
25	19017.0
26	33251.0
27	52792.0
28	77332.0
29	110129.0
30	148822.0
31	198855.0
32	263662.0
33	350834.0
34	482263.0
35	694774.0
36	1188585.0
37	2709536.0
38	2971913.0
39	60365.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.63687641477926	20.3051721791962	14.448300601378492	23.609650804646044
2	14.620644015411738	21.074447407000456	41.616786389284975	22.688122188302827
3	18.00773274038611	26.355810133477103	30.460520114124996	25.17593701201179
4	12.43760255616783	18.32005585965475	39.113680113050115	30.128661471127305
5	13.4258526637625	37.93060794293921	35.50743522886207	13.136104164436219
6	29.83833473451903	37.982241410859636	19.020797263998315	13.158626590623019
7	26.71277064484717	32.655504688312426	23.152058886381308	17.479665780459104
8	24.56193294196883	36.683138923592644	21.044067108362867	17.71086102607566
9	25.824832039282615	16.8728702333642	21.06316542877702	36.239132298576166
10-11	24.328040974463157	26.703249459967825	28.885041835198173	20.083667730370852
12-13	25.26083319572976	24.267896111283	28.85290230331055	21.618368389676686
14-15	22.350466872388214	25.62130084443585	26.48248945288288	25.545742830293054
16-17	21.881578043777488	28.733631001255304	27.16730747165536	22.21748348331184
18-19	21.941917432980997	27.947240863197166	28.755261901847216	21.35557980197462
20-21	22.8072964853529	27.069585081656673	28.647901436152445	21.475216996837982
22-23	22.542569902942645	27.021573158805705	28.43399172223745	22.001865216014195
24-25	22.273124586353852	27.1874195039926	28.51312441569565	22.026331493957898
26-27	22.188701139052974	27.345065131570102	28.445101257095747	22.02113247228118
28-29	22.142085162637766	27.332604635644397	28.483847011482062	22.041463190235778
30-31	22.404745913886178	27.227995871492872	28.351734921001576	22.015523293619374
32-33	22.196441078988983	27.382655013803873	28.30434158196197	22.116562325245166
34-35	22.363286394532338	27.370352643638913	28.304680605593973	21.961680356234776
36-37	22.375789061415126	27.29109406914721	28.216482597119636	22.116634272318027
38-39	22.237203600984127	27.430531012749	28.311805867230465	22.02045951903641
40-41	22.419814845304064	27.335192842233386	28.181426425948615	22.06356588651394
42-43	22.316416261628543	27.4176242045464	27.995084176287822	22.27087535753724
44-45	22.4385190776821	27.48798741311568	27.82943977604402	22.244053733158207
46-47	22.46096872890506	27.514826474092025	27.570197502524625	22.45400729447829
48-49	22.446616558923335	27.561195041302998	27.57906046033916	22.413127939434503
50-51	22.45297703851637	27.680155405535533	27.458734540848706	22.40813301509939
52-53	22.60204856030133	27.600419269366043	27.368017300434943	22.429514869897684
54-55	22.580572835560485	27.517269813575247	27.41707749142341	22.485079859440862
56-57	22.57958856488972	27.604149874057565	27.390271609332263	22.425989951720453
58-59	22.510473223324308	27.638244999986817	27.400058498930292	22.451223277758587
60-61	22.58960791987247	27.651531981894284	27.36619646626667	22.39266363196658
62-63	22.55741261381429	27.71228390870663	27.339008055499075	22.391295421980004
64-65	22.56192249952714	27.619003586724933	27.3273966072355	22.49167730651243
66-67	22.636084281359732	27.66140696817061	27.17860092302506	22.5239078274446
68-69	22.540889527903246	27.653672320805445	27.263380706872876	22.542057444418436
70-71	22.53743329339509	27.4652607162147	27.346276287351305	22.6510297030389
72-73	22.574024877327314	27.520085967169166	27.276889053436758	22.629000102066755
74-75	22.490614524624856	27.657482105091617	27.236799890582613	22.615103479700917
76-77	22.620639613640954	27.584441666573507	27.175665801722936	22.619252918062603
78-79	22.510474021784884	27.569765299293252	27.152004924127716	22.767755754794152
80-81	22.51982461560162	27.642245279888904	27.188319196426352	22.64961090808312
82-83	22.585736006915354	27.588935855912794	27.265631805628544	22.559696331543307
84-85	22.626477603188263	27.583153608833843	27.20050559081832	22.589863197159577
86-87	22.509166494665784	27.564775298451533	27.295224055390165	22.630834151492515
88-89	22.580298333679927	27.599475015493706	27.220625523749124	22.599601127077236
90-91	22.590725121486464	27.64082985652454	27.212467939829455	22.555977082159544
92-93	22.59405149749153	27.596981049076764	27.252216106456245	22.55675134697546
94-95	22.472910059465644	27.582878531417805	27.33862109255044	22.60559031656611
96-97	22.5898784953653	27.711698000329527	27.334430493339273	22.3639930109659
98-99	23.709342736976094	29.60898604800989	27.55555070909348	19.12612050592054
100	22.58004089008123	30.668518390112503	21.35270528857714	25.39873543122913
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1384.0
1	1267.5
2	1849.5
3	2706.0
4	3664.5
5	6028.0
6	7972.0
7	9019.5
8	9552.5
9	9233.0
10	8436.5
11	7717.0
12	8509.5
13	11596.5
14	19068.0
15	29830.5
16	39144.0
17	45401.5
18	47499.5
19	45112.5
20	40016.5
21	34262.5
22	30157.5
23	29622.5
24	33431.0
25	41612.5
26	53764.5
27	68035.5
28	84778.5
29	104937.5
30	126246.5
31	148718.0
32	174351.5
33	201052.0
34	226995.5
35	251333.0
36	277515.5
37	303091.0
38	322642.0
39	338965.5
40	354758.5
41	367816.0
42	375752.0
43	383450.5
44	390692.5
45	397634.5
46	401067.0
47	398561.5
48	395436.5
49	390051.5
50	382341.5
51	369847.5
52	353433.5
53	334637.0
54	311505.0
55	287586.5
56	262413.0
57	235372.5
58	207804.5
59	179525.5
60	150113.0
61	121154.0
62	95325.0
63	72972.5
64	53751.0
65	39185.0
66	29057.5
67	22175.5
68	16820.0
69	12028.0
70	8532.5
71	6053.5
72	4053.5
73	2586.5
74	1705.5
75	1130.5
76	766.0
77	502.0
78	330.0
79	239.0
80	168.0
81	111.5
82	88.5
83	60.5
84	40.5
85	32.5
86	25.5
87	19.0
88	11.0
89	4.5
90	1.5
91	1.5
92	2.0
93	2.0
94	1.5
95	1.0
96	1.0
97	1.0
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	1.7061592776462851E-4
7	0.0
8	0.0
9	0.0
10-11	0.003598929726285133
12-13	3.7322234198512484E-5
14-15	0.002404618231932733
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	141.0
20-21	498.0
22-23	1023.0
24-25	2508.0
26-27	3209.0
28-29	5623.0
30-31	13143.0
32-33	16594.0
34-35	23064.0
36-37	34686.0
38-39	43000.0
40-41	46191.0
42-43	55190.0
44-45	66432.0
46-47	65024.0
48-49	57347.0
50-51	50734.0
52-53	49609.0
54-55	52513.0
56-57	55761.0
58-59	56509.0
60-61	55857.0
62-63	54759.0
64-65	57601.0
66-67	61212.0
68-69	63232.0
70-71	62797.0
72-73	60910.0
74-75	62787.0
76-77	61595.0
78-79	67079.0
80-81	70207.0
82-83	67688.0
84-85	68806.0
86-87	73995.0
88-89	79697.0
90-91	86476.0
92-93	92131.0
94-95	114554.0
96-97	336776.0
98-99	605421.0
100-101	6475409.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.21607317643388
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.85865494354532	74.83326987085101
2	7.811242821942267	13.156643941831678
3	1.8896037347599686	4.7740501920302485
4	0.6932867805782982	2.3354356098174485
5	0.29358121533555814	1.2362128556962877
6	0.1579076642005403	0.7979018042059469
7	0.09055718577134941	0.5338459408500316
8	0.052977139006396785	0.35692212921926575
9	0.03527705750431891	0.26738057306096885
>10	0.11381777265727185	1.4604909601703286
>50	0.002447852414785255	0.13965693748213626
>100	6.038930581880907E-4	0.07878566030206728
>500	4.193922563402243E-5	0.02940352448257555
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	4.265398194115713E-5	0.0	1.0663495485289282E-5	0.0
3	0.0	4.265398194115713E-5	0.0	1.0663495485289282E-5	0.0
4	0.0	9.597145936760353E-5	0.0	2.1326990970578564E-5	0.0
5	0.0	9.597145936760353E-5	0.0	2.1326990970578564E-5	0.0
6	0.0	9.597145936760353E-5	0.0	2.1326990970578564E-5	0.0
7	0.0	1.0663495485289281E-4	0.0	2.1326990970578564E-5	0.0
8	0.0	1.172984503381821E-4	0.0	2.1326990970578564E-5	0.0
9	0.0	1.599524322793392E-4	0.0	6.398097291173569E-5	0.0
10-11	1.0663495485289282E-5	1.599524322793392E-4	0.0	7.464446839702498E-5	0.0
12-13	1.0663495485289282E-5	1.6528418002198386E-4	0.0	8.530796388231426E-5	0.0
14-15	1.0663495485289282E-5	2.665873871322321E-4	0.0	9.597145936760353E-5	0.0
16-17	1.0663495485289282E-5	2.879143781028106E-4	0.0	1.172984503381821E-4	0.0
18-19	1.0663495485289282E-5	3.145731168160338E-4	0.0	1.2796194582347139E-4	0.0
20-21	1.0663495485289282E-5	3.518953510145463E-4	0.0	1.3329369356611604E-4	0.0
22-23	1.0663495485289282E-5	3.945493329557034E-4	0.0	1.4395718905140529E-4	0.0
24-25	1.0663495485289282E-5	4.425350626395052E-4	0.0	1.599524322793392E-4	0.0
26-27	1.0663495485289282E-5	4.7452554909537307E-4	0.0	1.8661117099256244E-4	0.0
28-29	1.0663495485289282E-5	4.798572968380177E-4	0.0	2.66587387132232E-4	0.0
30-31	1.0663495485289282E-5	5.33174774264464E-4	0.0	6.291462336320676E-4	0.0
32-33	1.0663495485289282E-5	5.33174774264464E-4	0.0	0.0015408750976243011	0.0
34-35	1.0663495485289282E-5	6.984589542864479E-4	0.0	0.002703196105520833	0.0
36-37	1.5995243227933923E-5	8.637431343084317E-4	0.0	0.004393360139939184	5.331747742644641E-6
38-39	2.1326990970578564E-5	9.010653685069443E-4	0.0	0.007437788100989273	1.0663495485289282E-5
40-41	2.1326990970578564E-5	9.277241072201675E-4	0.0	0.010471552566554073	1.0663495485289282E-5
42-43	2.1326990970578564E-5	9.383876027054568E-4	0.0	0.013745245680537883	1.0663495485289282E-5
44-45	2.1326990970578564E-5	0.0010183638188451262	0.0	0.017322848415852438	1.0663495485289282E-5
46-47	2.6658738713223205E-5	0.0010770130440142173	0.0	0.02123101951121096	1.0663495485289282E-5
48-49	3.1990486455867847E-5	0.0010770130440142173	0.0	0.025027223903973942	1.0663495485289282E-5
50-51	3.1990486455867847E-5	0.0012103067375803336	0.0	0.029047361701928003	1.0663495485289282E-5
52-53	3.1990486455867847E-5	0.0012422972240362013	0.0	0.032832902599205696	1.0663495485289282E-5
54-55	3.1990486455867847E-5	0.0012529607195214906	0.0	0.036634438739711325	1.0663495485289282E-5
56-57	3.1990486455867847E-5	0.0012689559627494244	0.0	0.04087317819511381	1.0663495485289282E-5
58-59	3.1990486455867847E-5	0.0013009464492052922	0.0	0.045399832028619115	1.0663495485289282E-5
60-61	3.1990486455867847E-5	0.0013276051879185155	0.0	0.050033120816977306	1.0663495485289282E-5
62-63	3.1990486455867847E-5	0.0013436004311464494	0.0	0.05433050949754889	1.0663495485289282E-5
64-65	3.1990486455867847E-5	0.0014608988814846316	0.0	0.05823334884516476	1.0663495485289282E-5
66-67	3.1990486455867847E-5	0.0014608988814846316	0.0	0.062450761309596675	1.5995243227933923E-5
68-69	3.1990486455867847E-5	0.00148222587245521	0.0	0.06686544844050643	2.1326990970578564E-5
70-71	3.1990486455867847E-5	0.0014982211156831442	0.0	0.07093890371588694	2.1326990970578564E-5
72-73	4.265398194115713E-5	0.0015035528634257888	0.0	0.07420193333438546	2.1326990970578564E-5
74-75	4.265398194115713E-5	0.0015408750976243011	0.0	0.07631863718821538	2.1326990970578564E-5
76-77	4.265398194115713E-5	0.001620851313763971	0.0	0.07837136006913357	2.1326990970578564E-5
78-79	5.3317477426446404E-5	0.0016421783047345494	0.0	0.07981626370739027	2.1326990970578564E-5
80-81	5.3317477426446404E-5	0.0016528418002198387	0.0	0.08031211624745623	3.1990486455867847E-5
82-83	5.3317477426446404E-5	0.001663505295705128	0.0	0.08081330053526482	4.265398194115713E-5
84-85	5.3317477426446404E-5	0.001663505295705128	0.0	0.08109055141788234	4.265398194115713E-5
86-87	5.3317477426446404E-5	0.0016688370434477726	0.0	0.0812398403546764	4.265398194115713E-5
88	5.3317477426446404E-5	0.0017488132595874421	0.0	0.08124517210241904	4.265398194115713E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	12285	0.0	49.093678	1
GTATCAA	21045	0.0	37.37781	1
TCAACGC	25220	0.0	30.58671	4
ATCAACG	25625	0.0	30.188139	3
CAACGCA	25940	0.0	29.706266	5
TATCAAC	26665	0.0	29.141184	2
AACGCAG	26750	0.0	28.85552	6
ACGCAGA	31085	0.0	24.817444	7
CGCAGAG	32080	0.0	24.089012	8
GTACATG	30785	0.0	21.766455	1
TACATGG	30815	0.0	21.209042	2
GCAGAGT	37305	0.0	20.587494	9
ACATGGG	31270	0.0	20.399826	3
GAGTACT	19205	0.0	19.608904	12-13
GTGGTAT	6135	0.0	18.924347	1
CAGAGTA	35855	0.0	18.715267	10-11
CATGGGG	21690	0.0	18.483984	4
AGAGTAC	33230	0.0	18.394175	10-11
TGGTATC	6060	0.0	18.01048	2
GTACTTT	21470	0.0	17.45941	14-15
>>END_MODULE