FastQCFastQC Report
Fri 27 May 2016
SRR2139506_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139506_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7923435
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA118050.051.412381
GTATCAA212150.037.541931
TCAACGC258750.030.2819124
ATCAACG262100.029.9436053
CAACGCA264550.029.6032245
TATCAAC269200.029.2978692
AACGCAG271750.028.9774076
CTTATAC57150.028.3396421
ACGCAGA310500.025.3888117
CGCAGAG318900.024.6259658
GTGGTAT55100.022.670921
GTACATG287000.022.0326581
TATACAC90500.021.7991543
TGGTATC54400.021.458192
GCAGAGT367500.021.3117719
TACATGG295100.020.8732242
ACATGGG293850.020.7274783
GAGTACT197600.020.33984412-13
TTATACA88450.019.8207132
CAGAGTA357800.019.12005410-11
Produced by FastQC (version 0.11.5)