##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139506_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7923435
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.51063951430156	33.0	33.0	33.0	33.0	33.0
2	32.541344252839835	33.0	33.0	33.0	33.0	33.0
3	32.55690770480227	33.0	33.0	33.0	33.0	33.0
4	36.463248326010124	37.0	37.0	37.0	37.0	37.0
5	36.489838687387476	37.0	37.0	37.0	37.0	37.0
6	36.48074402074353	37.0	37.0	37.0	37.0	37.0
7	36.45697756086849	37.0	37.0	37.0	37.0	37.0
8	36.450141384386946	37.0	37.0	37.0	37.0	37.0
9	36.4893370110312	37.0	37.0	37.0	37.0	37.0
10-11	36.40823948199234	37.0	37.0	37.0	37.0	37.0
12-13	36.409967078672324	37.0	37.0	37.0	37.0	37.0
14-15	38.37226865620782	40.0	37.0	40.0	37.0	40.0
16-17	38.324221376208676	40.0	37.0	40.0	37.0	40.0
18-19	38.329726059467895	40.0	37.0	40.0	37.0	40.0
20-21	38.330310284650864	40.0	37.0	40.0	37.0	40.0
22-23	38.331641912444596	40.0	37.0	40.0	37.0	40.0
24-25	38.31798962272387	40.0	37.0	40.0	37.0	40.0
26-27	38.20452111939094	40.0	37.0	40.0	37.0	40.0
28-29	38.13500166046018	40.0	37.0	40.0	35.0	40.0
30-31	37.99291980161421	40.0	37.0	40.0	33.0	40.0
32-33	37.99616153166025	40.0	37.0	40.0	33.0	40.0
34-35	37.9522779162755	40.0	37.0	40.0	33.0	40.0
36-37	37.867403850102235	40.0	37.0	40.0	33.0	40.0
38-39	37.81231119397702	40.0	37.0	40.0	33.0	40.0
40-41	37.822087421267284	40.0	37.0	40.0	33.0	40.0
42-43	37.756703944901915	40.0	37.0	40.0	33.0	40.0
44-45	37.693800409383044	40.0	37.0	40.0	33.0	40.0
46-47	37.6553440275865	40.0	37.0	40.0	33.0	40.0
48-49	37.633316554470085	40.0	37.0	40.0	33.0	40.0
50-51	37.54219966936196	40.0	37.0	40.0	33.0	40.0
52-53	37.44661178354602	40.0	37.0	40.0	33.0	40.0
54-55	37.29327472065867	40.0	37.0	40.0	33.0	40.0
56-57	37.18338356279223	38.5	37.0	40.0	33.0	40.0
58-59	37.02417127652731	37.0	37.0	40.0	33.0	40.0
60-61	36.908045601257115	37.0	37.0	40.0	33.0	40.0
62-63	36.74624441845994	37.0	37.0	40.0	33.0	40.0
64-65	36.57316953569381	37.0	37.0	40.0	33.0	40.0
66-67	36.348503970817305	37.0	37.0	40.0	33.0	40.0
68-69	36.12042500981988	37.0	37.0	40.0	33.0	40.0
70-71	35.87037233953181	37.0	35.0	37.0	33.0	40.0
72-73	35.66220525317593	37.0	33.0	37.0	33.0	40.0
74-75	35.44059597738623	37.0	33.0	37.0	33.0	40.0
76-77	33.75035776153807	35.0	33.0	37.0	27.0	37.0
78-79	34.94700194985402	37.0	33.0	37.0	33.0	37.0
80-81	35.018042288604796	37.0	33.0	37.0	33.0	37.0
82-83	34.864477509152294	37.0	33.0	37.0	33.0	37.0
84-85	34.731807434960956	37.0	33.0	37.0	33.0	37.0
86-87	34.61483923229095	37.0	33.0	37.0	33.0	37.0
88-89	34.445772389395	37.0	33.0	37.0	30.0	37.0
90-91	34.45316074694665	37.0	33.0	37.0	33.0	37.0
92-93	34.38703517321411	37.0	33.0	37.0	33.0	37.0
94-95	34.36186359113196	37.0	33.0	37.0	33.0	37.0
96-97	34.3652741913238	37.0	33.0	37.0	33.0	37.0
98-99	34.36611959660956	37.0	33.0	37.0	33.0	37.0
100	34.37816926981272	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	17.0
20	83.0
21	347.0
22	1390.0
23	3843.0
24	8694.0
25	16718.0
26	29066.0
27	44902.0
28	67532.0
29	94310.0
30	129150.0
31	171441.0
32	226832.0
33	303895.0
34	414848.0
35	595759.0
36	1012014.0
37	2276404.0
38	2472518.0
39	53670.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.51544121962255	20.503960719056824	14.532383997597002	23.448214063723626
2	14.55205980739414	21.056612441447427	41.9284691551076	22.46285859605083
3	17.908634323371114	26.756703374231	30.63498343836985	24.699678864028037
4	12.31475994944112	18.525980209341025	38.98576816746777	30.173491673750085
5	13.240557914591337	38.049116323917595	35.48620011396572	13.224125647525348
6	29.429960185167303	38.08123982684769	19.062352928258715	13.426447059726287
7	26.37811757148257	32.90728074376833	23.03942671328786	17.675174971461242
8	24.52149856722495	36.6856294018945	21.091874925458466	17.700997105422083
9	25.729648820240214	16.91248808124254	21.012868787337815	36.34499431117943
10-11	24.24114777847973	26.79324610936802	28.769608716414076	20.19599739573818
12-13	25.2930453251766	24.348972219491223	28.79873534615614	21.559247109176034
14-15	22.3796503977119	25.640403972581744	26.469702718975352	25.51024291073101
16-17	21.78223838524579	28.893598546589956	27.05132937924019	22.27283368892406
18-19	21.821268174724725	27.87299321569496	28.905411604941545	21.400327004638772
20-21	22.745539531616902	26.980575435602844	28.806040224832973	21.46784480794728
22-23	22.49585631793892	27.053221835473657	28.612463062173944	21.83845878441348
24-25	22.2296251502328	27.27857705553816	28.678803540949165	21.81299425327987
26-27	22.132825329246433	27.435150611071123	28.659357698720772	21.772666360961665
28-29	21.968001739572877	27.26638340486897	28.83021115506161	21.935403700496543
30-31	22.26826375008118	27.39136458711894	28.532498599477684	21.8078730633222
32-33	22.251111946788736	27.34078989780631	28.59445415272087	21.81364400268408
34-35	22.227877188773675	27.343515151761448	28.62381368996918	21.80479396949569
36-37	22.17415298814041	27.405979033168936	28.459086238427368	21.96078174026329
38-39	22.089461107919952	27.334066051262983	28.62042580219522	21.95604703862184
40-41	22.217494664439666	27.25844375434975	28.538637078134126	21.98542450307646
42-43	22.203312398392438	27.314787169925598	28.35045083285332	22.13144959882864
44-45	22.22996034181088	27.473534619701105	28.139808899764013	22.156696138723998
46-47	22.433969084965348	27.400479047621427	27.728977117126103	22.436574750287118
48-49	22.28373839681256	27.538850644981206	27.731571548655374	22.445839409550857
50-51	22.257731894020242	27.641660585943434	27.71830375413898	22.382303765897344
52-53	22.44239648997137	27.62103610174737	27.64214780220811	22.294419606073152
54-55	22.439506436802688	27.61072880513605	27.543622181181792	22.406142576879475
56-57	22.496451035603858	27.754690456228055	27.40462228462739	22.3442362235407
58-59	22.31814131370532	27.87029110338507	27.561101419420535	22.250466163489076
60-61	22.4631291895032	27.93421833563512	27.36298651645237	22.239665958409315
62-63	22.437300813554412	28.01108846803924	27.3401094334854	22.21150128492095
64-65	22.428119687828413	27.875136984362697	27.346640549590777	22.350102778218112
66-67	22.500496575663792	27.838318504515918	27.238823329885925	22.42236158993437
68-69	22.423295850538018	27.796342399736762	27.337714196922924	22.4426475528023
70-71	22.4545783104773	27.598006888488275	27.411093288331617	22.53632151270281
72-73	22.50605957550821	27.665868496579364	27.305066297177344	22.523005630735078
74-75	22.381192732784555	27.75637987050863	27.318322722310178	22.544104674396635
76-77	22.49072727107862	27.706655258689473	27.248586541452173	22.554030928779735
78-79	22.44440363342292	27.700186050196614	27.194365561991923	22.661044754388545
80-81	22.426062462637926	27.76489841265366	27.234743151398845	22.574295973309567
82-83	22.491412819688588	27.69265007588319	27.387346606686556	22.428590497741666
84-85	22.552683960976335	27.7478480854939	27.232987308697503	22.46648064483226
86-87	22.391105295161335	27.722040165598415	27.345759275770433	22.541095263469817
88-89	22.485160734682626	27.704576277609366	27.30466059652442	22.505602391183587
90-91	22.44457922215431	27.83625137563728	27.260281192452346	22.45888820975607
92-93	22.53033352469396	27.696330110769463	27.335968760775977	22.437367603760602
94-95	22.353143175494296	27.774764238248444	27.35558922332457	22.51650336293269
96-97	22.4790022827116	27.869099115733782	27.406188310435958	22.24571029111866
98-99	23.58642264988481	29.756227923550938	27.58958603962497	19.06776338693928
100	22.51487251714327	30.783825713592073	21.431693884900895	25.269607884363758
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1977.0
1	1742.0
2	2192.0
3	3022.0
4	3974.0
5	6479.5
6	8594.0
7	9877.5
8	10525.5
9	10115.5
10	9320.0
11	8527.5
12	9185.5
13	12250.5
14	19913.5
15	31092.0
16	40780.0
17	46868.5
18	48587.0
19	45661.0
20	39810.0
21	33536.0
22	29124.5
23	28647.5
24	32259.0
25	39690.5
26	50562.5
27	63335.5
28	77809.0
29	94918.5
30	113234.0
31	132311.0
32	153419.5
33	174403.0
34	195350.5
35	216046.0
36	237534.0
37	257233.0
38	272461.5
39	284841.0
40	295251.5
41	303818.0
42	309264.5
43	314996.0
44	320428.0
45	325552.5
46	331399.5
47	335354.0
48	334463.0
49	329407.5
50	324460.0
51	316630.5
52	303705.5
53	287514.5
54	268788.0
55	248058.5
56	226652.0
57	204456.5
58	182032.5
59	157117.0
60	129442.5
61	103916.0
62	81835.5
63	62540.5
64	45717.5
65	33272.5
66	24896.5
67	18688.0
68	13887.0
69	9871.5
70	6644.0
71	4467.0
72	2803.5
73	1675.5
74	1029.5
75	660.5
76	453.0
77	305.0
78	199.5
79	135.0
80	92.5
81	61.0
82	46.5
83	36.0
84	27.5
85	22.0
86	15.5
87	10.5
88	7.0
89	3.5
90	1.5
91	1.5
92	2.0
93	1.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	7.572473302298813E-5
7	0.0
8	0.0
9	0.0
10-11	0.003388681802778719
12-13	2.5241577674329376E-5
14-15	0.0022023276520852383
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	136.0
20-21	472.0
22-23	1041.0
24-25	2696.0
26-27	3345.0
28-29	5822.0
30-31	13615.0
32-33	16969.0
34-35	23433.0
36-37	34654.0
38-39	42747.0
40-41	45444.0
42-43	54315.0
44-45	64007.0
46-47	62693.0
48-49	55508.0
50-51	48754.0
52-53	45674.0
54-55	51382.0
56-57	54469.0
58-59	54954.0
60-61	54742.0
62-63	53912.0
64-65	55963.0
66-67	60561.0
68-69	56895.0
70-71	56372.0
72-73	53251.0
74-75	54374.0
76-77	53355.0
78-79	58857.0
80-81	60622.0
82-83	58763.0
84-85	58972.0
86-87	62413.0
88-89	68036.0
90-91	73553.0
92-93	78732.0
94-95	96290.0
96-97	278771.0
98-99	496230.0
100-101	5350641.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.13419444384395
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.67574874507007	74.60662687371175
2	7.962407878158293	13.398215453243296
3	1.948975974917182	4.919265709201876
4	0.6844268819357048	2.3033481746948965
5	0.2999683243825631	1.2618796665298309
6	0.15183416470068395	0.7664667081687582
7	0.08294428951301125	0.4884915687325922
8	0.04925345270845033	0.33151196537627464
9	0.03555579661218693	0.2692312475197952
>10	0.10546204301319008	1.3593073936247864
>50	0.0024229215875481275	0.13786608041528978
>100	9.25703883301789E-4	0.11466360962890279
>500	7.382351776874768E-5	0.043125549151954885
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.262078883716469E-5	0.0	1.262078883716469E-5	0.0
3	0.0	1.262078883716469E-5	0.0	1.262078883716469E-5	0.0
4	0.0	8.834552186015282E-5	0.0	2.524157767432938E-5	0.0
5	0.0	8.834552186015282E-5	0.0	3.7862366511494065E-5	0.0
6	1.6407025488314095E-4	8.834552186015282E-5	0.0	5.048315534865876E-5	0.0
7	1.6407025488314095E-4	8.834552186015282E-5	0.0	5.048315534865876E-5	0.0
8	1.6407025488314095E-4	1.135870995344822E-4	0.0	5.048315534865876E-5	0.0
9	1.6407025488314095E-4	1.8931183255747034E-4	0.0	8.834552186015282E-5	0.0
10-11	1.7038064930172331E-4	1.8931183255747034E-4	0.0	9.465591627873517E-5	0.0
12-13	1.7669104372030565E-4	1.8931183255747034E-4	0.0	1.0096631069731752E-4	0.0
14-15	1.7669104372030565E-4	2.9027814325478785E-4	0.0	1.0096631069731752E-4	1.8931183255747036E-5
16-17	2.6503656558045846E-4	3.028989320919525E-4	0.0	1.135870995344822E-4	2.524157767432938E-5
18-19	2.776573544176232E-4	3.092093265105349E-4	0.0	1.135870995344822E-4	2.524157767432938E-5
20-21	2.776573544176232E-4	3.1551972092911724E-4	0.0	1.262078883716469E-4	2.524157767432938E-5
22-23	2.776573544176232E-4	3.6600287627777596E-4	0.0	1.5144946604597629E-4	3.786236651149407E-5
24-25	2.839677488362055E-4	4.354172148821818E-4	0.0	1.7038064930172331E-4	5.048315534865876E-5
26-27	2.9027814325478785E-4	4.669691869750935E-4	0.0	2.3979498790612912E-4	5.048315534865876E-5
28-29	2.9027814325478785E-4	4.669691869750935E-4	0.0	6.184186530210698E-4	5.048315534865876E-5
30-31	2.9027814325478785E-4	5.553147088352464E-4	0.0	0.0022969835683639738	5.67935497672411E-5
32-33	2.9027814325478785E-4	5.742458920909933E-4	0.0	0.005546836693933881	6.310394418582345E-5
34-35	2.9027814325478785E-4	6.625914139511462E-4	0.0	0.00950345399438501	6.310394418582345E-5
36-37	2.9027814325478785E-4	7.320057525555519E-4	0.0	0.015466776719945327	6.310394418582345E-5
38-39	2.9027814325478785E-4	7.446265413927167E-4	0.0	0.024124637862240304	8.834552186015282E-5
40-41	2.9027814325478785E-4	7.951096967413754E-4	0.0	0.031968458124538156	8.834552186015282E-5
42-43	2.9027814325478785E-4	8.203512744157048E-4	0.0	0.03864485541939828	8.834552186015282E-5
44-45	2.9027814325478785E-4	9.465591627873517E-4	0.0	0.0455358061244902	8.834552186015282E-5
46-47	3.028989320919525E-4	0.0010475254734846691	0.0	0.05223744499702465	8.834552186015282E-5
48-49	3.028989320919525E-4	0.0010601462623218338	0.0	0.05856046020444416	8.834552186015282E-5
50-51	3.6600287627777596E-4	0.0011989749395306453	0.0	0.0646941635793062	1.0096631069731752E-4
52-53	3.786236651149407E-4	0.0012242165172049748	0.0	0.06986237660812514	1.135870995344822E-4
54-55	3.786236651149407E-4	0.0012368373060421395	0.0	0.07513786634205998	1.262078883716469E-4
56-57	3.786236651149407E-4	0.001262078883716469	0.0	0.07942262415227738	1.262078883716469E-4
58-59	3.786236651149407E-4	0.001281010066972216	0.0	0.08378310669551779	1.262078883716469E-4
60-61	3.912444539521054E-4	0.0012999412502279631	0.0	0.08812465805550244	1.262078883716469E-4
62-63	4.4172760930076413E-4	0.0013251828279022923	0.0	0.09251669257083575	1.262078883716469E-4
64-65	4.4172760930076413E-4	0.0014513907162739394	0.0	0.09629661882756657	1.262078883716469E-4
66-67	4.4172760930076413E-4	0.0014577011106925216	0.0	0.10049934151034243	1.3882867720881156E-4
68-69	4.4172760930076413E-4	0.001482942688366851	0.0	0.10478409932055983	1.6407025488314095E-4
70-71	4.669691869750935E-4	0.0015144946604597627	0.0	0.10837471374473319	1.7669104372030565E-4
72-73	5.300731311609169E-4	0.00153342584371551	0.0	0.11142263424890847	1.7669104372030565E-4
74-75	5.300731311609169E-4	0.0015902193934827509	0.0	0.11393417122750424	1.7669104372030565E-4
76-77	5.300731311609169E-4	0.0016974960985986507	0.0	0.1160923261186594	1.8300143813888798E-4
78-79	5.300731311609169E-4	0.001760600042784474	0.0	0.11802330681074559	1.8931183255747034E-4
80-81	5.300731311609169E-4	0.0017669104372030565	0.0	0.11874269177446398	2.0193262139463504E-4
82-83	5.300731311609169E-4	0.0017669104372030565	0.0	0.11924121293353199	2.0193262139463504E-4
84-85	5.300731311609169E-4	0.0017669104372030565	0.0	0.11947469752701953	2.0193262139463504E-4
86-87	5.363835255794993E-4	0.0017669104372030565	0.0	0.11962614699306551	2.0193262139463504E-4
88	5.553147088352464E-4	0.00183001438138888	0.0	0.11964507817632125	2.0193262139463504E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11805	0.0	51.41238	1
GTATCAA	21215	0.0	37.54193	1
TCAACGC	25875	0.0	30.281912	4
ATCAACG	26210	0.0	29.943605	3
CAACGCA	26455	0.0	29.603224	5
TATCAAC	26920	0.0	29.297869	2
AACGCAG	27175	0.0	28.977407	6
CTTATAC	5715	0.0	28.339642	1
ACGCAGA	31050	0.0	25.388811	7
CGCAGAG	31890	0.0	24.625965	8
GTGGTAT	5510	0.0	22.67092	1
GTACATG	28700	0.0	22.032658	1
TATACAC	9050	0.0	21.799154	3
TGGTATC	5440	0.0	21.45819	2
GCAGAGT	36750	0.0	21.311771	9
TACATGG	29510	0.0	20.873224	2
ACATGGG	29385	0.0	20.727478	3
GAGTACT	19760	0.0	20.339844	12-13
TTATACA	8845	0.0	19.820713	2
CAGAGTA	35780	0.0	19.120054	10-11
>>END_MODULE