FastQCFastQC Report
Fri 27 May 2016
SRR2139505_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139505_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11193234
Sequences flagged as poor quality0
Sequence length19-101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA130650.048.389831
GTATCAA221900.032.8088721
TCAACGC261550.027.8014684
CTTATAC145900.027.470931
ATCAACG265550.027.3514563
CAACGCA270100.026.9530835
TATCAAC277550.026.2767832
AACGCAG280550.026.1337836
GTGGTAT67950.023.7953111
TATACAC193800.023.1973293
TGGTATC65150.022.6513292
ACGCAGA327600.022.3021437
CGCAGAG338950.021.6180158
TTATACA196400.021.3438932
GTACATG305250.019.9406011
TACATGG311750.019.3601882
ACATGGG309700.019.073993
GAGTACT230600.018.54805812-13
GCAGAGT399650.018.3346089
TACACAT245600.018.0776735
Produced by FastQC (version 0.11.5)