##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139505_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	11193234
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.188328770755618	28.0	18.0	32.0	18.0	33.0
2	28.166250165055068	29.0	27.0	32.0	18.0	33.0
3	30.156048555761455	31.0	29.0	33.0	25.0	33.0
4	30.239203075715203	32.0	29.0	33.0	27.0	33.0
5	31.707587726657014	33.0	32.0	33.0	30.0	33.0
6	35.54502863068886	38.0	36.0	38.0	29.0	38.0
7	35.91499132422319	38.0	36.0	38.0	31.0	38.0
8	36.37069402819596	38.0	37.0	38.0	33.0	38.0
9	36.57412209911809	38.0	38.0	38.0	34.0	38.0
10-11	36.72114748070129	38.0	38.0	38.0	34.0	38.0
12-13	36.78269962014552	38.0	38.0	38.0	34.5	38.0
14-15	36.80779598639678	38.0	38.0	38.0	35.0	38.0
16-17	36.83053700119197	38.0	38.0	38.0	35.0	38.0
18-19	36.841367249179285	38.0	38.0	38.0	35.0	38.0
20-21	36.85545105623906	38.0	38.0	38.0	35.0	38.0
22-23	36.8753565094751	38.0	38.0	38.0	35.0	38.0
24-25	36.88745193116807	38.0	38.0	38.0	35.0	38.0
26-27	36.845757623518594	38.0	38.0	38.0	35.0	38.0
28-29	36.83218566483874	38.0	38.0	38.0	35.0	38.0
30-31	36.81112816529178	38.0	38.0	38.0	35.0	38.0
32-33	36.78452450075412	38.0	38.0	38.0	35.0	38.0
34-35	36.7579415730343	38.0	38.0	38.0	35.0	38.0
36-37	36.736952523792354	38.0	38.0	38.0	35.0	38.0
38-39	36.70414455275096	38.0	38.0	38.0	34.0	38.0
40-41	36.73554252912553	38.0	38.0	38.0	35.0	38.0
42-43	36.70158153852601	38.0	38.0	38.0	34.0	38.0
44-45	36.66285520995419	38.0	38.0	38.0	34.0	38.0
46-47	36.62905690669838	38.0	38.0	38.0	34.0	38.0
48-49	36.59414754823942	38.0	38.0	38.0	34.0	38.0
50-51	36.57241620187092	38.0	38.0	38.0	34.0	38.0
52-53	36.5422303307061	38.0	38.0	38.0	34.0	38.0
54-55	36.474336794389245	38.0	38.0	38.0	34.0	38.0
56-57	36.481437983813926	38.0	38.0	38.0	34.0	38.0
58-59	36.471461324400366	38.0	38.0	38.0	34.0	38.0
60-61	36.430739329699904	38.0	38.0	38.0	34.0	38.0
62-63	36.43398661805346	38.0	38.0	38.0	34.0	38.0
64-65	36.45849983466735	38.0	38.0	38.0	34.0	38.0
66-67	36.43524080941987	38.0	38.0	38.0	34.0	38.0
68-69	36.41382889316713	38.0	38.0	38.0	34.0	38.0
70-71	36.41079681134175	38.0	38.0	38.0	34.0	38.0
72-73	36.39527215905743	38.0	38.0	38.0	34.0	38.0
74-75	36.385498461178315	38.0	38.0	38.0	34.0	38.0
76-77	36.35302633294137	38.0	38.0	38.0	33.5	38.0
78-79	36.35045968621637	38.0	38.0	38.0	34.0	38.0
80-81	36.34208490598154	38.0	38.0	38.0	33.5	38.0
82-83	36.31229489197294	38.0	38.0	38.0	33.0	38.0
84-85	36.27396965337179	38.0	37.0	38.0	33.0	38.0
86-87	36.29164447471665	38.0	37.0	38.0	33.0	38.0
88-89	36.291406100748716	38.0	37.0	38.0	33.0	38.0
90-91	36.28244160602539	38.0	37.0	38.0	33.0	38.0
92-93	36.26887208273898	38.0	37.0	38.0	33.0	38.0
94-95	36.25567499257835	38.0	37.0	38.0	33.0	38.0
96-97	36.25110314244996	38.0	37.0	38.0	33.0	38.0
98-99	36.294212220062064	38.0	37.0	38.0	33.0	38.0
100-101	35.78110581866245	38.0	36.0	38.0	31.5	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	12.0
18	77.0
19	170.0
20	293.0
21	723.0
22	2280.0
23	6566.0
24	15416.0
25	28880.0
26	49275.0
27	75004.0
28	106373.0
29	144374.0
30	186665.0
31	239129.0
32	305486.0
33	399992.0
34	559939.0
35	899831.0
36	2103608.0
37	6069141.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	39.81447184969063	22.816730178248754	13.100682072759312	24.26811589930131
2	15.888616283729975	20.71197653868399	40.65296052954848	22.746446648037555
3	18.7235692153089	26.18643366687174	29.48295680642235	25.607040311397018
4	13.535855678528655	18.04697373431128	36.89651266113082	31.520657926029244
5	13.95545918000235	37.21044020159612	35.50653551685934	13.327565101542183
6	31.24151608016057	37.395805358844456	17.72039251569296	13.64228604530201
7	27.255786511122025	32.29041793390056	22.055676871305618	18.398118683671797
8	24.39430820440277	37.67978941564163	20.31825654676745	17.60764583318816
9	25.192460016470662	16.77597377129791	20.68806030500211	37.343505907229314
10-11	24.583888114189705	26.64955573382794	28.39223804274252	20.374318109239837
12-13	25.164822784907383	23.87269845336924	28.675336368381117	22.287142393342265
14-15	22.21745247485266	25.53615434755574	25.821675097952046	26.424718079639554
16-17	22.12795247557587	28.801372329033768	26.65190864409696	22.4187665512934
18-19	22.180801366253935	27.814445762502597	28.238903073052885	21.765849798190587
20-21	22.7294993592433	26.89863482728873	28.322486986528027	22.049378826939943
22-23	22.484171586537588	27.091902073464425	28.145786470461807	22.27813986953618
24-25	22.457058571632487	27.16542231294541	28.267356115248532	22.110163000173568
26-27	22.46224502343511	27.230249593209965	28.2911118879919	22.016393495363022
28-29	22.449368490748586	27.127786639507516	28.27952194221302	22.14332292753088
30-31	22.45048104941785	27.257224826369388	28.353389329185635	21.938904795027128
32-33	22.351018869265342	27.39931316297655	28.228684929800817	22.020983037957283
34-35	22.614379632106242	27.131303887232573	28.32905973269957	21.92525674796162
36-37	22.2246008825101	27.31636742577585	28.25527348081389	22.20375821090016
38-39	22.112312736755293	27.097480829428562	28.49038345877875	22.299822975037394
40-41	22.343369059386614	26.992125065897234	28.464990948027523	22.199514926688625
42-43	22.436296754105676	26.97929120797263	28.296700366850587	22.28771167107111
44-45	22.232988411380113	27.201553161125712	28.21944675057995	22.346011676914223
46-47	22.546456495669005	27.018273278235156	27.852934673807837	22.582335552288004
48-49	22.39195346347772	27.10432489580008	27.854119591936378	22.649602048785823
50-51	22.22524967694885	27.18671665505549	28.045478257006646	22.542555410989014
52-53	22.38756719085221	27.144128513005022	28.152660488143216	22.315643807999553
54-55	22.340483213088362	27.12672828429535	27.912679973665533	22.62010852895075
56-57	22.52635177075738	27.34801169182611	27.659296922729297	22.466339614687218
58-59	22.215448057933216	27.56272711409468	27.989879339350914	22.23194548862119
60-61	22.461008572128947	27.84058751871148	27.53578854502763	22.162615364131945
62-63	22.179890645900887	28.15616526056394	27.553642328514943	22.110301765020225
64-65	22.17140431008475	28.030311165483173	27.536104957896207	22.262179566535867
66-67	22.237036871722047	28.063154291173447	27.382290676731802	22.3175181603727
68-69	22.193080578767123	27.891763131046936	27.510897177894872	22.404259112291065
70-71	22.2605133561623	27.49681267324308	27.644233742746614	22.598440227848002
72-73	22.38573497881721	27.363886351939847	27.592434418600902	22.657944250642036
74-75	22.34259714806025	27.391570134597792	27.632059588905683	22.633773128436275
76-77	22.531160705488226	27.369162202481274	27.501504987960097	22.598172104070404
78-79	22.4360545715969	27.35731883993119	27.456304675772408	22.750321912699505
80-81	22.474388804790664	27.453000523158664	27.439238995811145	22.633371676239523
82-83	22.507377636908785	27.43780288666487	27.51151794266267	22.543301533763668
84-85	22.560052969408872	27.45617060113354	27.442314551470215	22.541461877987377
86-87	22.51965370480996	27.442963215436322	27.50056228201973	22.536820797733988
88-89	22.558985385924057	27.43499983381726	27.487292770406636	22.51872200985205
90-91	22.54200239901536	27.522184028778877	27.48588994972165	22.44992362248411
92-93	22.627986137244108	27.43536367961487	27.51908276785533	22.417567415285692
94-95	22.519630196272196	27.501140009882068	27.53878712473434	22.440442669111388
96-97	22.573351544233986	27.513638420810178	27.50284120571958	22.41016882923625
98-99	22.7726709095511	28.063620228739683	27.59903029865295	21.564678563056273
100-101	23.408802024297408	30.56937881840465	24.90069976141812	21.121119395879823
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1863.0
1	1811.5
2	2353.0
3	3078.5
4	3780.0
5	5380.5
6	7047.5
7	9009.0
8	10903.0
9	11995.0
10	11906.0
11	11270.0
12	12127.0
13	15083.5
14	21449.5
15	29147.0
16	34570.0
17	37313.5
18	37864.5
19	36428.0
20	34232.0
21	32917.5
22	33709.5
23	38654.5
24	48222.5
25	62429.5
26	80202.0
27	99480.5
28	120495.5
29	142869.0
30	164487.5
31	187794.5
32	214092.5
33	241699.5
34	268918.5
35	294670.0
36	322944.5
37	348749.0
38	370558.5
39	390123.5
40	407496.5
41	425271.0
42	437896.5
43	453841.0
44	489621.0
45	511415.0
46	502686.0
47	491327.5
48	482242.5
49	476466.0
50	471477.5
51	461132.5
52	445670.0
53	424254.0
54	399537.0
55	374331.5
56	345315.5
57	313744.0
58	281778.5
59	245343.0
60	205398.5
61	167560.5
62	133360.0
63	104219.5
64	79953.0
65	59938.5
66	44415.5
67	33612.5
68	25633.5
69	19137.5
70	14205.0
71	10218.0
72	7055.0
73	4746.0
74	3151.5
75	2024.0
76	1301.0
77	824.5
78	507.5
79	313.0
80	202.5
81	125.5
82	88.5
83	63.0
84	39.5
85	26.5
86	20.5
87	16.0
88	10.0
89	6.5
90	6.0
91	3.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0028588699208825616
4	0.0
5	0.04930657216672143
6	0.0
7	0.001965473070606761
8	0.0
9	0.0
10-11	4.466984251379003E-6
12-13	0.0
14-15	1.786793700551601E-5
16-17	0.0
18-19	0.0
20-21	6.253895842079481E-5
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	6.671195562809307E-4
48-49	1.8490470248691136E-4
50-51	6.551118328498895E-4
52-53	0.001310659502177791
54-55	0.002907521685791752
56-57	0.002925904549752218
58-59	0.006866879957614775
60-61	0.00597736187416623
62-63	0.0067370309894389255
64-65	0.004057141366557658
66-67	0.0066026844365207685
68-69	0.006881055289822903
70-71	0.003532613623582093
72-73	0.0027300761033894135
74-75	0.0039004577500169344
76-77	0.0021715435104394526
78-79	0.0
80-81	5.405300161557803E-4
82-83	2.452517587338053E-4
84-85	0.0
86-87	0.0
88-89	0.0
90-91	3.489814076083647E-5
92-93	7.826352423121092E-4
94-95	1.071548610385997E-4
96-97	0.0
98-99	1.245282170666295E-5
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	122.0
20-21	455.0
22-23	903.0
24-25	1171.0
26-27	3377.0
28-29	8862.0
30-31	25888.0
32-33	32862.0
34-35	46051.0
36-37	70937.0
38-39	77825.0
40-41	75125.0
42-43	83306.0
44-45	98241.0
46-47	98612.0
48-49	92048.0
50-51	82881.0
52-53	74042.0
54-55	80653.0
56-57	81933.0
58-59	89389.0
60-61	87241.0
62-63	86478.0
64-65	101454.0
66-67	110588.0
68-69	106638.0
70-71	122188.0
72-73	87338.0
74-75	87758.0
76-77	92332.0
78-79	97011.0
80-81	96195.0
82-83	90430.0
84-85	89775.0
86-87	93449.0
88-89	98252.0
90-91	100143.0
92-93	97430.0
94-95	102928.0
96-97	165750.0
98-99	921371.0
100-101	7233802.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.54047251228512
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.56340959381963	75.71732966872841
2	7.278394319875277	12.306377898659761
3	1.7093301897884654	4.335227457726926
4	0.6439941459292448	2.177742775680154
5	0.3050048289778907	1.2892626180159796
6	0.1588814908871907	0.8059149787835655
7	0.09084056863880365	0.537579321720639
8	0.06506564639934996	0.44005443927346477
9	0.043152575070974974	0.3283325177959872
>10	0.13824589679941698	1.7827052833954256
>50	0.0028336247972723322	0.15716551039779836
>100	8.10937476657871E-4	0.10104884128556693
>500	3.6181539798275616E-5	0.02125868853624418
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.786793700551601E-5	0.0	0.0	0.0
3	0.0	2.6801905508274018E-5	0.0	8.933968502758005E-6	0.0
4	0.0	3.573587401103202E-5	0.0	8.933968502758005E-6	0.0
5	8.933968502758005E-6	4.4669842513790025E-5	0.0	1.786793700551601E-5	0.0
6	8.933968502758005E-6	4.4669842513790025E-5	0.0	1.786793700551601E-5	0.0
7	8.933968502758005E-6	4.4669842513790025E-5	0.0	2.6801905508274018E-5	0.0
8	8.933968502758005E-6	5.3603811016548036E-5	0.0	2.6801905508274018E-5	2.6801905508274018E-5
9	8.933968502758005E-6	1.2507555903861208E-4	0.0	4.4669842513790025E-5	2.6801905508274018E-5
10-11	2.6801905508274018E-5	1.2507555903861208E-4	0.0	5.3603811016548036E-5	2.6801905508274018E-5
12-13	2.6801905508274018E-5	1.2507555903861208E-4	0.0	5.3603811016548036E-5	2.6801905508274018E-5
14-15	2.6801905508274018E-5	2.0548127556343414E-4	0.0	7.147174802206404E-5	3.573587401103202E-5
16-17	5.3603811016548036E-5	2.1888222831757114E-4	0.0	8.933968502758005E-5	3.573587401103202E-5
18-19	6.253777951930604E-5	2.635520708313612E-4	0.0	1.0274063778171707E-4	5.3603811016548036E-5
20-21	6.253777951930604E-5	3.037549290937722E-4	0.0	1.2060857478723308E-4	5.3603811016548036E-5
22-23	6.253777951930604E-5	3.126888975965302E-4	0.0	1.474104802955071E-4	5.807079526792704E-5
24-25	6.700476377068504E-5	3.752266771158362E-4	0.0	1.742123858037811E-4	7.147174802206404E-5
26-27	7.147174802206404E-5	4.0649556687548925E-4	0.0	2.8588699208825617E-4	7.147174802206404E-5
28-29	7.593873227344304E-5	4.109625511268682E-4	0.0	8.219251022537366E-4	7.147174802206404E-5
30-31	9.827365353033807E-5	4.556323936406583E-4	0.0	0.0025104451492749995	7.147174802206404E-5
32-33	1.1614159053585407E-4	4.556323936406583E-4	0.0	0.006512863038510586	7.147174802206404E-5
34-35	1.3400952754137008E-4	5.092362046572064E-4	0.0	0.011949182872438832	7.147174802206404E-5
36-37	1.518774645468861E-4	5.807079526792704E-4	0.0	0.020324778343774462	7.147174802206404E-5
38-39	1.518774645468861E-4	6.343117636958184E-4	0.0	0.034757604459980024	7.593873227344304E-5
40-41	1.518774645468861E-4	6.968495432151244E-4	0.0	0.0723115410613233	8.040571652482205E-5
42-43	1.518774645468861E-4	7.013165274665034E-4	0.0	0.08527026237457377	8.040571652482205E-5
44-45	1.608114330496441E-4	7.951231967454625E-4	0.0	0.1026870339707005	8.040571652482205E-5
46-47	1.697454015524021E-4	8.844628817730425E-4	0.0	0.12191740117288712	1.0720762203309607E-4
48-49	1.697454015524021E-4	8.844628817730425E-4	0.0	0.14006675818624	1.1614159053585407E-4
50-51	1.697454015524021E-4	9.291327242868326E-4	0.0	0.1574299259713502	1.1614159053585407E-4
52-53	1.697454015524021E-4	9.291327242868326E-4	0.0	0.18458025625123176	1.2954254328999108E-4
54-55	1.697454015524021E-4	9.425336770409696E-4	0.0	0.24655519575486406	1.3400952754137008E-4
56-57	1.697454015524021E-4	9.648685982978646E-4	0.0	0.3156013713284293	1.474104802955071E-4
58-59	1.697454015524021E-4	9.916705038061386E-4	0.0	0.3923084248931095	1.518774645468861E-4
60-61	1.831463543065391E-4	9.916705038061386E-4	0.0	0.4130754346777705	1.518774645468861E-4
62-63	1.876133385579181E-4	9.916705038061386E-4	0.0	0.4296702811716435	1.518774645468861E-4
64-65	1.920803228092971E-4	0.0010720762203309607	0.0	0.4384791741153629	1.518774645468861E-4
66-67	2.010142913120551E-4	0.0010810101888337185	0.0	0.4441567110988656	1.518774645468861E-4
68-69	2.054812755634341E-4	0.0011167460628447507	0.0	0.4502809465075063	1.518774645468861E-4
70-71	2.054812755634341E-4	0.0011212130470961298	0.0	0.4560076203177741	1.518774645468861E-4
72-73	2.2334921256895015E-4	0.0011256800313475088	0.0	0.4606666848919624	1.786793700551601E-4
74-75	2.2334921256895015E-4	0.0011301470155988878	0.0	0.4643564138836015	1.786793700551601E-4
76-77	2.2334921256895015E-4	0.0011926847951181938	0.0	0.46729568952100886	1.786793700551601E-4
78-79	2.4121714957446615E-4	0.0012373546376319836	0.0	0.4704047105599687	1.786793700551601E-4
80-81	2.4121714957446615E-4	0.0012418216218833627	0.0	0.47171800392987406	2.099482598148131E-4
82-83	2.4121714957446615E-4	0.0012462886061347417	0.0	0.4722183061660285	2.4121714957446615E-4
84-85	2.4121714957446615E-4	0.0012686235273916368	0.0	0.4726784055439206	2.4121714957446615E-4
86-87	2.456841338258452E-4	0.0012998924171512898	0.0	0.47282581602421603	2.456841338258452E-4
88-89	2.5015111807722416E-4	0.0013668971809219748	0.0	0.4728570849139757	2.5461810232860313E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	13065	0.0	48.38983	1
GTATCAA	22190	0.0	32.808872	1
TCAACGC	26155	0.0	27.801468	4
CTTATAC	14590	0.0	27.47093	1
ATCAACG	26555	0.0	27.351456	3
CAACGCA	27010	0.0	26.953083	5
TATCAAC	27755	0.0	26.276783	2
AACGCAG	28055	0.0	26.133783	6
GTGGTAT	6795	0.0	23.795311	1
TATACAC	19380	0.0	23.197329	3
TGGTATC	6515	0.0	22.651329	2
ACGCAGA	32760	0.0	22.302143	7
CGCAGAG	33895	0.0	21.618015	8
TTATACA	19640	0.0	21.343893	2
GTACATG	30525	0.0	19.940601	1
TACATGG	31175	0.0	19.360188	2
ACATGGG	30970	0.0	19.07399	3
GAGTACT	23060	0.0	18.548058	12-13
GCAGAGT	39965	0.0	18.334608	9
TACACAT	24560	0.0	18.077673	5
>>END_MODULE