FastQCFastQC Report
Fri 27 May 2016
SRR2139504_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139504_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14102676
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA182600.047.3672521
GTATCAA319650.035.4587941
TCAACGC385000.029.730084
ATCAACG387750.029.5086573
CAACGCA392900.029.1761115
TATCAAC397750.028.8749522
AACGCAG407050.028.232736
ACGCAGA469550.024.4198237
CGCAGAG484600.023.6611778
GTGGTAT93450.021.6422021
TGGTATC91500.020.9277152
GCAGAGT567750.020.1731439
GAGTACT327150.019.4503612-13
GTACATG403400.018.7421231
CAGAGTA552000.018.38791510-11
TACATGG410300.018.3535232
ACATGGG410000.018.094073
AGAGTAC513700.017.56529610-11
GTACTTT361500.017.47746714-15
AGTACTT359150.015.71748312-13
Produced by FastQC (version 0.11.5)