##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139504_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	14102676
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.757669182784884	28.0	18.0	32.0	18.0	33.0
2	27.957684626662346	29.0	25.0	32.0	18.0	33.0
3	30.048462575471493	31.0	29.0	33.0	25.0	33.0
4	30.154445510908708	31.0	29.0	33.0	25.0	33.0
5	31.69054468811451	33.0	32.0	33.0	30.0	33.0
6	35.564043944567686	38.0	36.0	38.0	29.0	38.0
7	35.94592090182034	38.0	36.0	38.0	31.0	38.0
8	36.40061999580789	38.0	37.0	38.0	33.0	38.0
9	36.59834488149625	38.0	38.0	38.0	34.0	38.0
10-11	36.74629155487937	38.0	38.0	38.0	34.0	38.0
12-13	36.80559132890808	38.0	38.0	38.0	34.5	38.0
14-15	36.828786820316935	38.0	38.0	38.0	35.0	38.0
16-17	36.852580566978915	38.0	38.0	38.0	35.0	38.0
18-19	36.86274207817013	38.0	38.0	38.0	35.0	38.0
20-21	36.875539905985576	38.0	38.0	38.0	35.0	38.0
22-23	36.892572301920936	38.0	38.0	38.0	35.0	38.0
24-25	36.90443936679418	38.0	38.0	38.0	35.0	38.0
26-27	36.862667931596974	38.0	38.0	38.0	35.0	38.0
28-29	36.84999121896758	38.0	38.0	38.0	35.0	38.0
30-31	36.829894224943914	38.0	38.0	38.0	35.0	38.0
32-33	36.81341317173744	38.0	38.0	38.0	35.0	38.0
34-35	36.797208094505585	38.0	38.0	38.0	35.0	38.0
36-37	36.77787128807363	38.0	38.0	38.0	35.0	38.0
38-39	36.766524972801626	38.0	38.0	38.0	35.0	38.0
40-41	36.7576269425259	38.0	38.0	38.0	35.0	38.0
42-43	36.71475452082872	38.0	38.0	38.0	34.5	38.0
44-45	36.67645123444828	38.0	38.0	38.0	34.0	38.0
46-47	36.64522806022124	38.0	38.0	38.0	34.0	38.0
48-49	36.60790464088177	38.0	38.0	38.0	34.0	38.0
50-51	36.58915881783396	38.0	38.0	38.0	34.0	38.0
52-53	36.5621135255401	38.0	38.0	38.0	34.0	38.0
54-55	36.495695719986315	38.0	38.0	38.0	34.0	38.0
56-57	36.50508625552861	38.0	38.0	38.0	34.0	38.0
58-59	36.501249087691924	38.0	38.0	38.0	34.0	38.0
60-61	36.460750748909945	38.0	38.0	38.0	34.0	38.0
62-63	36.465970577781846	38.0	38.0	38.0	34.0	38.0
64-65	36.48935665350095	38.0	38.0	38.0	34.0	38.0
66-67	36.47095731258711	38.0	38.0	38.0	34.0	38.0
68-69	36.45623316774595	38.0	38.0	38.0	34.0	38.0
70-71	36.456673329990394	38.0	38.0	38.0	34.0	38.0
72-73	36.44640982833227	38.0	38.0	38.0	34.0	38.0
74-75	36.43805487045058	38.0	38.0	38.0	34.0	38.0
76-77	36.40448518638546	38.0	38.0	38.0	34.0	38.0
78-79	36.40010267249432	38.0	38.0	38.0	34.0	38.0
80-81	36.3910346673938	38.0	38.0	38.0	34.0	38.0
82-83	36.36199427152081	38.0	38.0	38.0	33.5	38.0
84-85	36.32080177884136	38.0	38.0	38.0	33.0	38.0
86-87	36.33661876687187	38.0	38.0	38.0	33.0	38.0
88-89	36.33277256468553	38.0	38.0	38.0	33.0	38.0
90-91	36.32278912610364	38.0	38.0	38.0	33.0	38.0
92-93	36.309217800676635	38.0	37.0	38.0	33.0	38.0
94-95	36.29415146826351	38.0	37.0	38.0	33.0	38.0
96-97	36.289978113573184	38.0	37.0	38.0	33.0	38.0
98-99	36.33179885927795	38.0	37.0	38.0	33.5	38.0
100-101	35.819803157559534	38.0	36.0	38.0	31.5	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	2.0
18	36.0
19	87.0
20	134.0
21	592.0
22	2531.0
23	7800.0
24	17979.0
25	35192.0
26	59264.0
27	91641.0
28	130980.0
29	176221.0
30	230106.0
31	295172.0
32	376869.0
33	498558.0
34	697916.0
35	1121520.0
36	2659350.0
37	7700726.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	39.27213530254826	23.400700689713073	13.729139065522034	23.598024942216643
2	15.543815939613163	20.993398699651046	40.989802219096575	22.47298314163922
3	18.455528503106066	26.827024227010828	29.447899570529067	25.26954769935404
4	13.430642524865494	18.460141890801435	37.93386446657358	30.175351117759497
5	13.982650528765351	37.28287532684472	35.42355747944364	13.31091666494629
6	30.473968202914115	37.97736684867468	18.277970790791763	13.270694157619447
7	26.911538556175596	32.653307237629434	22.67733072461386	17.757823481581106
8	24.309365116237515	37.5406128595736	20.433902048093564	17.716119976095317
9	25.236706849111474	17.355954288391793	21.167039503708374	36.240299358788356
10-11	24.381958586510766	26.915874934511542	28.51946072218708	20.182705756790618
12-13	25.167274110504778	24.246948743874878	28.854521257331633	21.731255888288715
14-15	22.23378149659997	25.74620782882791	26.374294433402802	25.645716241169314
16-17	21.820500591518943	28.905365194520527	27.107752457760498	22.16638175620003
18-19	21.831012780836772	28.047825816887517	28.840944796576196	21.280216605699515
20-21	22.594973252002355	27.104105791490596	28.844557765907116	21.456363190599937
22-23	22.37424789273813	26.966179709243814	28.694679057408063	21.964893340609994
24-25	22.134518398743307	27.151114456149124	28.744527449848807	21.969839695258763
26-27	22.072178960555586	27.27979920045465	28.702266442413315	21.94575539657644
28-29	22.01124088795387	27.279816867717795	28.759253954133555	21.94968829019478
30-31	22.3084382474183	27.130361897304823	28.645938171065062	21.915261684211814
32-33	22.0520448234639	27.305316100058697	28.641557669786028	22.001081406691377
34-35	22.206502472871506	27.272937517809186	28.657742706014094	21.862817303305214
36-37	22.193847546533668	27.20337127414395	28.580056113838914	22.022725065483467
38-39	22.074926507303903	27.32202427558745	28.677269943547266	21.92577927356138
40-41	22.31574278641863	27.18923664182824	28.539506264130498	21.955514307622625
42-43	22.2016464056688	27.260488826280117	28.38406560386819	22.153799164182892
44-45	22.22352042398066	27.34197132465142	28.302280764857734	22.132227486510185
46-47	22.29437172295489	27.338089826289536	28.059379504295773	22.308158946459802
48-49	22.24462971949444	27.413784856387657	28.053549645820443	22.28803577829746
50-51	22.217100983021908	27.539043826133213	27.947564597893283	22.296290592951596
52-53	22.353458148070693	27.524983886129796	27.816697839530825	22.30486012626869
54-55	22.344523779924636	27.43516865936045	27.8473334602998	22.372974100415114
56-57	22.324881298185783	27.47112287763494	27.88047684609274	22.32351897808654
58-59	22.282220410907076	27.489952571440295	27.953014759114897	22.27481225853773
60-61	22.3513866664138	27.481888519377335	27.9123272251724	22.254397589036465
62-63	22.352702074915083	27.56258305826462	27.8847138192922	22.200001047528094
64-65	22.230590844468534	27.535322454151085	27.85696961666002	22.377117084720357
66-67	22.297603305458143	27.61959794584496	27.70303457070813	22.37976417798877
68-69	22.219246580213646	27.575226944780283	27.797008956745845	22.408517518260222
70-71	22.24829838819363	27.371173083843846	27.819547974866417	22.560980553096112
72-73	22.306155614919632	27.385311271300505	27.75441601676796	22.554117097011908
74-75	22.235181199025906	27.473137022022097	27.732271843058264	22.55940993589373
76-77	22.420352374231108	27.434787986087755	27.629041354612276	22.51581828506886
78-79	22.360787470133065	27.40639186207479	27.59419953703364	22.638621130758512
80-81	22.349238167699387	27.494620945589276	27.582686581753723	22.573454304957608
82-83	22.413979395893147	27.480655868597008	27.653160115444276	22.45220462006557
84-85	22.54062041918758	27.492211971819785	27.55214072255755	22.415026886435083
86-87	22.41529114791027	27.540064119678675	27.60611678666858	22.43852794574248
88-89	22.44568616506614	27.56289853185044	27.573025686096713	22.41838961698671
90-91	22.48803616716286	27.60497582679412	27.558191323564017	22.348796682479
92-93	22.574983950555847	27.527379261680494	27.571091517660644	22.326545270103015
94-95	22.39030531418324	27.589763666819977	27.60632439213881	22.41360662685797
96-97	22.43026297640047	27.57909374444658	27.607712916294552	22.382930362858396
98-99	22.63946453305565	28.139184880242325	27.700040254640378	21.521310332061642
100-101	23.283477212866792	30.635048622836347	25.021366986214648	21.060107178082216
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1939.0
1	2004.5
2	2895.5
3	3956.0
4	5178.5
5	7559.5
6	9664.5
7	12041.5
8	14389.0
9	15469.0
10	15281.5
11	14771.0
12	16209.5
13	20648.5
14	29894.5
15	41182.0
16	49546.5
17	53941.5
18	54052.0
19	50748.5
20	46145.5
21	42262.5
22	41939.0
23	46949.0
24	57364.5
25	74197.0
26	96428.0
27	121918.0
28	152095.5
29	186510.0
30	220826.0
31	255228.5
32	292147.0
33	328540.5
34	363626.0
35	397645.5
36	433470.0
37	466649.5
38	492155.5
39	512718.5
40	532668.5
41	550892.5
42	560519.5
43	569418.5
44	581668.0
45	593593.5
46	599175.5
47	598280.5
48	595575.5
49	589113.5
50	579223.0
51	566132.5
52	545054.0
53	516716.0
54	486761.0
55	454569.0
56	419263.5
57	379569.5
58	338843.0
59	293974.0
60	242817.5
61	195231.5
62	153942.5
63	118643.0
64	89293.0
65	66105.0
66	49011.0
67	36725.0
68	27374.0
69	20177.5
70	14720.5
71	10491.0
72	7256.5
73	4739.0
74	2982.5
75	1835.0
76	1155.5
77	702.5
78	446.0
79	300.5
80	204.5
81	134.0
82	84.5
83	53.5
84	36.5
85	27.5
86	21.0
87	17.0
88	12.0
89	8.0
90	5.0
91	2.5
92	2.0
93	1.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.003127066097242821
4	0.0
5	0.04903324730710682
6	0.0
7	0.0016096235920048082
8	0.0
9	0.0
10-11	3.545426414107507E-6
12-13	3.545426414107507E-6
14-15	2.127255848464504E-5
16-17	0.0
18-19	0.0
20-21	6.0273146554652214E-5
22-23	3.5456686554913097E-6
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	3.6845838522302067E-6
46-47	6.135053726338677E-4
48-49	1.800766698931654E-4
50-51	6.242811308190568E-4
52-53	0.0012582100589318902
54-55	0.002976288085638446
56-57	0.002871626380818836
58-59	0.006670403132639758
60-61	0.005905558125603392
62-63	0.006875914267466558
64-65	0.003968769941368626
66-67	0.006660921319259654
68-69	0.0068057914432455065
70-71	0.0035472350352256485
72-73	0.0027293879179344093
74-75	0.0037223883044571575
76-77	0.002344068560874339
78-79	0.0
80-81	5.612819749388462E-4
82-83	1.9629484322977777E-4
84-85	0.0
86-87	0.0
88-89	0.0
90-91	1.3641120667170843E-5
92-93	7.312918638720893E-4
94-95	1.1164521458140465E-4
96-97	4.712526016678006E-6
98-99	4.8622585365943795E-6
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	111.0
20-21	591.0
22-23	1220.0
24-25	1500.0
26-27	3744.0
28-29	9521.0
30-31	28392.0
32-33	36772.0
34-35	50390.0
36-37	80465.0
38-39	93418.0
40-41	93268.0
42-43	103628.0
44-45	121300.0
46-47	121559.0
48-49	114821.0
50-51	105314.0
52-53	92591.0
54-55	99229.0
56-57	99735.0
58-59	109379.0
60-61	108234.0
62-63	103180.0
64-65	109952.0
66-67	116169.0
68-69	117691.0
70-71	117447.0
72-73	108516.0
74-75	109367.0
76-77	114197.0
78-79	120177.0
80-81	119461.0
82-83	114249.0
84-85	111841.0
86-87	116620.0
88-89	121120.0
90-91	126116.0
92-93	121643.0
94-95	130140.0
96-97	208299.0
98-99	1169866.0
100-101	9271443.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	82.11289474888717
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.00352622868489	72.26224286746935
2	8.105727283849632	13.31169462643856
3	2.0704053547189503	5.100209309387087
4	0.8045493749502494	2.642555125822912
5	0.38105986875019465	1.5644964447854748
6	0.20908045966462188	1.0300921067094029
7	0.12038638854656278	0.6919692396345782
8	0.07659830031972427	0.5031766537677737
9	0.05182734537902792	0.38301240206001097
>10	0.17194147674901689	2.136213010238704
>50	0.003760624738460967	0.2037246006723079
>100	0.0010889844264219835	0.14056028891714403
>500	4.830922223101414E-5	0.03005332409672356
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	7.090852828215014E-6	0.0
3	0.0	0.0	0.0	7.090852828215014E-6	0.0
4	0.0	3.545426414107507E-5	0.0	2.127255848464504E-5	0.0
5	0.0	4.254511696929008E-5	0.0	5.672682262572011E-5	0.0
6	4.96359697975051E-5	4.254511696929008E-5	0.0	5.672682262572011E-5	0.0
7	4.96359697975051E-5	4.254511696929008E-5	0.0	7.090852828215014E-5	0.0
8	4.96359697975051E-5	4.254511696929008E-5	0.0	7.799938111036516E-5	7.090852828215014E-6
9	4.96359697975051E-5	7.799938111036516E-5	0.0	8.509023393858016E-5	7.090852828215014E-6
10-11	4.96359697975051E-5	9.218108676679518E-5	0.0	1.2054449807965523E-4	7.090852828215014E-6
12-13	4.96359697975051E-5	9.218108676679518E-5	0.0	1.2763535090787025E-4	7.090852828215014E-6
14-15	4.96359697975051E-5	1.8790759994769787E-4	0.0	1.4181705656430028E-4	1.063627924232252E-5
16-17	6.736310186804263E-5	2.056347320182354E-4	0.0	1.453624829784078E-4	2.127255848464504E-5
18-19	7.090852828215014E-5	2.2336186408877294E-4	0.0	1.559987622207303E-4	2.8363411312860056E-5
20-21	7.090852828215014E-5	2.552707018157405E-4	0.0	1.7727132070537535E-4	3.545426414107507E-5
22-23	7.090852828215014E-5	2.8363411312860056E-4	0.0	1.8790759994769787E-4	3.545426414107507E-5
24-25	7.090852828215014E-5	3.119975244414606E-4	0.0	2.3399814333109546E-4	3.545426414107507E-5
26-27	7.090852828215014E-5	3.2972465651199813E-4	0.0	3.1554295085556815E-4	3.545426414107507E-5
28-29	7.090852828215014E-5	3.3681550934021315E-4	0.0	5.885407847418462E-4	3.545426414107507E-5
30-31	7.090852828215014E-5	3.7936062630950326E-4	0.0	0.0017266226636703558	3.545426414107507E-5
32-33	7.090852828215014E-5	3.899969055518258E-4	0.0	0.004722507983591199	4.609054338339759E-5
34-35	7.090852828215014E-5	4.573600074198684E-4	0.0	0.008597659054210704	4.96359697975051E-5
36-37	7.090852828215014E-5	5.24723109287911E-4	0.0	0.014465339769558628	4.96359697975051E-5
38-39	7.090852828215014E-5	5.530865206007711E-4	0.0	0.025636978400411382	5.672682262572011E-5
40-41	7.090852828215014E-5	6.346313281252437E-4	0.0	0.05954898205134969	5.672682262572011E-5
42-43	7.090852828215014E-5	6.771764450945337E-4	0.0	0.07030580579175186	6.0272249039827614E-5
44-45	7.799938111036516E-5	7.551758262048991E-4	0.0	0.08540223146302163	6.736310186804263E-5
46-47	7.799938111036516E-5	8.296297809011566E-4	0.0	0.10222882522437586	7.799938111036516E-5
48-49	7.799938111036516E-5	8.402660601434791E-4	0.0	0.11724016066170703	9.218108676679518E-5
50-51	8.509023393858016E-5	9.537197053949193E-4	0.0	0.13265567471024647	9.218108676679518E-5
52-53	8.509023393858016E-5	9.71446837465457E-4	0.0	0.15772538488440066	1.1345364525144022E-4
54-55	8.509023393858016E-5	9.749922638795644E-4	0.0	0.21617528474737702	1.3118077732197776E-4
56-57	8.509023393858016E-5	9.85628543121887E-4	0.0	0.2810069521557469	1.3827163015019277E-4
58-59	8.509023393858016E-5	9.85628543121887E-4	0.0	0.3535463765883865	1.453624829784078E-4
60-61	9.572651318090269E-5	0.001013991954434747	0.0	0.37309585783577526	1.5599876222073032E-4
62-63	1.063627924232252E-4	0.0010388099393334996	0.0	0.3849482183381367	1.630896150489453E-4
64-65	1.063627924232252E-4	0.0011558090109990474	0.0	0.3937018761545681	1.630896150489453E-4
66-67	1.1345364525144022E-4	0.0011699907166554772	0.0	0.3995837385755725	1.6663504146305282E-4
68-69	1.1345364525144022E-4	0.0011912632751401224	0.0	0.4053096022343561	1.7018046787716033E-4
70-71	1.1699907166554773E-4	0.00122317211286709	0.0	0.4105249244895082	1.7727132070537535E-4
72-73	1.3472620373608526E-4	0.0012408992449376276	0.0	0.4147085276581551	1.9145302636180538E-4
74-75	1.3472620373608526E-4	0.0012905352147351327	0.0	0.4181617729854958	1.9145302636180538E-4
76-77	1.3472620373608526E-4	0.0013472620373608526	0.0	0.4211115677620333	1.9145302636180538E-4
78-79	1.3472620373608526E-4	0.0013685345958454978	0.0	0.42370681989715997	1.9145302636180538E-4
80-81	1.3472620373608526E-4	0.0013685345958454978	0.0	0.4251001724779042	1.949984527759129E-4
82-83	1.3472620373608526E-4	0.001372080022259605	0.0	0.42558235047022286	1.985438791900204E-4
84-85	1.3472620373608526E-4	0.0013898071543301427	0.0	0.426050346756885	1.985438791900204E-4
86-87	1.3472620373608526E-4	0.0013898071543301427	0.0	0.4262311635040045	2.056347320182354E-4
88-89	1.453624829784078E-4	0.0014571702561981853	0.0	0.42626661776814556	2.1627101126055792E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	18260	0.0	47.367252	1
GTATCAA	31965	0.0	35.458794	1
TCAACGC	38500	0.0	29.73008	4
ATCAACG	38775	0.0	29.508657	3
CAACGCA	39290	0.0	29.176111	5
TATCAAC	39775	0.0	28.874952	2
AACGCAG	40705	0.0	28.23273	6
ACGCAGA	46955	0.0	24.419823	7
CGCAGAG	48460	0.0	23.661177	8
GTGGTAT	9345	0.0	21.642202	1
TGGTATC	9150	0.0	20.927715	2
GCAGAGT	56775	0.0	20.173143	9
GAGTACT	32715	0.0	19.45036	12-13
GTACATG	40340	0.0	18.742123	1
CAGAGTA	55200	0.0	18.387915	10-11
TACATGG	41030	0.0	18.353523	2
ACATGGG	41000	0.0	18.09407	3
AGAGTAC	51370	0.0	17.565296	10-11
GTACTTT	36150	0.0	17.477467	14-15
AGTACTT	35915	0.0	15.717483	12-13
>>END_MODULE