FastQCFastQC Report
Fri 27 May 2016
SRR2139503_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139503_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10443046
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA151600.051.8027041
GTATCAA262700.035.2334141
TCAACGC317700.029.3938984
ATCAACG319750.029.1786233
TATCAAC326500.028.6954542
CAACGCA328450.028.4451125
AACGCAG341800.027.385366
GTGGTAT71350.025.5175041
TGGTATC69200.025.3661652
ACGCAGA392900.023.8125787
CGCAGAG402900.023.2428288
CGAGACC82600.022.63730888-89
AGACCGA84500.022.23913490-91
ACCGAGG87800.022.02581292-93
GAGACCG86750.021.9045988-89
GACCGAG87200.021.90329790-91
ACGAGAC89350.021.22003786-87
ATCTCCG85550.021.0481174-75
TCTCCGA86650.020.49433976-77
GAGTACT271800.020.46243312-13
Produced by FastQC (version 0.11.5)